Band 3

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Solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
Atomic microscope image of Band 3
Identifiers
Symbol(s)	SLC4A1; WD; AE1; BND3; CD233; DI; EMPB3; EPB3; FR; MGC116750; MGC116753; MGC126619; MGC126623; RTA1A; SW; WD1; WR
External IDs	OMIM: 109270 MGI: 109393 Homologene: 37290
Gene ontology Molecular Function: • inorganic anion exchanger activity • protein binding • anion transmembrane transporter activity Cellular Component: • integral to plasma membrane • membrane • cortical cytoskeleton Biological Process: • anion transport • cell ion homeostasis
RNA expression pattern
More reference expression data
Orthologs
	Human	Mouse
Entrez	6521	20533
Ensembl	ENSG00000004939	ENSMUSG00000006574
Uniprot	P02730	Q3TA19
Refseq	NM_000342 (mRNA) NP_000333 (protein)	NM_011403 (mRNA) NP_035533 (protein)
Location	Chr 17: 39.68 - 39.7 Mb	Chr 11: 102.16 - 102.18 Mb
Pubmed search	[1]	[2]

solute carrier family 4 (anion exchanger), member 1, adaptor protein
Identifiers
Symbol	SLC4A1AP
Entrez	22950
HUGO	13813
OMIM	602655
RefSeq	NM_018158
UniProt	Q9BWU0
Other data
Locus	Chr. 2 p23.3

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Overview

Anion Exchanger 1 (AE1) or Band 3 is a phylogenetically preserved transport protein responsible for catalysing the electroneutral exchange of chloride (Cl-) for bicarbonate (HCO3-) across a plasma membrane.

It is ubiquitous throughout the vertebrates. In humans it is present in two specific sites:

• the erythrocyte (red blood cell) cell membrane and
• the basolateral surface of the alpha-intercalated cell (the acid secreting cell type) in the collecting duct of the kidney.

The erythrocyte and kidney forms are different isoforms of the same protein.

Discovery

AE1 was discovered following SDS-PAGE gel electrophoresis of erythrocyte cell membrane. The large 'third' band on the electrophoresis gel represented AE1, which was thus initially termed 'Band 3'. The chloride-bicarbonate exchanger in the red cell membrane is not a pump, which would use metabolic energy. Nor is it strictly an enzyme. It is protein counter-transporter, known as band III. ^[1]

AE1 in Red Cells

AE1 is an important structural component of the erythrocyte cell membrane, making up to 25% of the cell membrane surface, indeed each red cell contains approximately one million copies of AE1.

Function

Here it performs two functions:

• Electroneutral chloride and bicarbonate exchange across the plasma membrane on a one-for-one basis.This is crucial for CO₂ uptake by the red cell and conversion (by hydration catalysed by carbonic anhydrase) into a proton and a bicarbonate ion. The bicarbonate is then extruded from the cell by the band 3 molecule.

• Physical linkage of the plasma membrane to the underlying membrane skeleton (via binding with ankyrin and protein 4.2). This appears to be to prevent membrane surface loss, rather than being to do with membrane skeleton assembly.

Pathology

Mutations of erythroid AE1 affecting the extracellular domains of the molecule may cause alterations in the individual's blood group, as band 3 determines the Diego blood group.

More importantly erythroid AE1 mutations cause between 15-25% of cases of Hereditary spherocytosis (a disorder associated with progressive red cell membrane loss), and also cause the hereditary conditions of Hereditary stomatocytosis ^[1] and Southeast Asian Ovalocytosis ^[1]

AE1 in Alpha-Intercalated cells

A different isoform of AE1, known as kAE1 (which is 65 amino acids shorter than erythroid AE1) is found in the basolateral surface of the alpha-intercalated cell in the cortical collecting duct of the kidney.

Function

This is the principal acid secreting cell of the kidney, which generates hydrogen ions and bicarbonate ions from carbon dioxide and water-a reaction catalysed by Carbonic anhydrase.

The hydrogen ions are pumped into the collecting duct tubule by vacuolar H+ATPase, the apical proton pump,which thus excretes acid into the urine.

kAE1 exchanges bicarbonate for chloride on the basolateral surface, essentially returning bicarbonate to the blood.

Pathology

Mutations of kidney AE1 cause distal (type1) renal tubular acidosis, which is an inability to acidify the urine, even if the blood is too acid. These mutations are disease causing as they cause mistargetting of the mutant band 3 proteins so that they are retained within the cell or occasionally addressed to the wrong (ie apical) surface.

See also

• Cluster of differentiation

References

External links

• Diego blood group system at BGMUT Blood Group Antigen Gene Mutation Database at NCBI, NIH
• MeSH Band+3+Protein
• MeSH Chloride-Bicarbonate+Antiporters

Further reading