Nuclear factor Y gene transcriptions

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Associate Editor(s)-in-Chief: Henry A. Hoff

The protein encoded by this gene is one subunit of a trimeric complex NF-Y, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes.[1]

Subunit NFYA associates with a tight dimer composed of the NFYB and NFYC subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the NFYA subunit, suggesting a role as the regulatory subunit.

There is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation.

Regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.[2]

Nuclear transcription factor Y subunit beta is a protein that in humans is encoded by the NFYB gene.[3][4]

Nuclear transcription factor Y subunit gamma is a protein that in humans is encoded by the NFYC gene.[5][6][7]

NF-Y complex serves as a pioneer factor by promoting chromatin accessibility to facilitate other co-localizing cell type-specific transcription factors.[1]

NF-Y has also been implicated as a central player in transcription start site (TSS) selection in animals.[8]

It safeguards the integrity of the nucleosome-depleted region and PIC localization at protein-coding gene promoters. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.[9]

Additional regulation, preliminarily supported by the EST database, may be represented by alternative splicing in this subunit C.[7]

Two microRNAs; miR-30c and miR-30e are located within introns of the nfyc gene. These microRNAs are actively transcribed in human insulin-producing beta cells in the Islets of Langerhans that also show high expression of nfyc and CDH1 genes. The expression of these intronic microRNAs is essential for maintaining the differentiated phenotype of human islet beta cells. Inhibition of miR-30 family microRNAs induces epithelial-mesenchymal transition of human pancreatic islet cells.[10]

Human genes

Nuclear transcription factor Y subunit alpha is a protein that in humans is encoded by the NFYA gene.[11][4]

Nuclear transcription factor Y subunit beta is a protein that in humans is encoded by the NFYB gene.[11][4]

Nuclear transcription factor Y subunit gamma is a protein that in humans is encoded by the NFYC gene.[5][6][7]

Interactions

NFYA has been shown to interact with:

NFYB has been shown to interact with:

NFYC has been shown to interact with:

NFYA, NFYB and NFYC form the NFY complex and it has been shown that the NFY complex serves as a pioneer factor by promoting chromatin accessibility to facilitate other co-localizing cell type-specific transcription factors.[1]

Consensus sequences

Consensus sequences for the nuclear factor Y are CAATGGCG, CCAATGGAA and CCAATGGCA.[19]

NFY samplings

Copying an apparent consensus sequence for the NFY CCAATGG(A/C)(A/G) and putting it in "⌘F" finds none located between ZSCAN22 and none between ZNF497 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence CCAATGG(A/C)(A/G) (starting with SuccessablesNFY.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

  1. negative strand, negative direction, looking for CCAATGG(A/C)(A/G), 0.
  2. positive strand, negative direction, looking for CCAATGG(A/C)(A/G), 0.
  3. positive strand, positive direction, looking for CCAATGG(A/C)(A/G), 0.
  4. negative strand, positive direction, looking for CCAATGG(A/C)(A/G), 0.
  5. complement, negative strand, negative direction, looking for GGTTACC(G/T)(C/T), 0.
  6. complement, positive strand, negative direction, looking for GGTTACC(G/T)(C/T), 0.
  7. complement, positive strand, positive direction, looking for GGTTACC(G/T)(C/T), 0.
  8. complement, negative strand, positive direction, looking for GGTTACC(G/T)(C/T), 0.
  9. inverse complement, negative strand, negative direction, looking for (C/T)(G/T)CCATTGG, 0.
  10. inverse complement, positive strand, negative direction, looking for (C/T)(G/T)CCATTGG, 0.
  11. inverse complement, positive strand, positive direction, looking for (C/T)(G/T)CCATTGG, 0.
  12. inverse complement, negative strand, positive direction, looking for (C/T)(G/T)CCATTGG, 0.
  13. inverse negative strand, negative direction, looking for (A/G)(A/C)GGTAACC, 0.
  14. inverse positive strand, negative direction, looking for (A/G)(A/C)GGTAACC, 0.
  15. inverse positive strand, positive direction, looking for (A/G)(A/C)GGTAACC, 0.
  16. inverse negative strand, positive direction, looking for (A/G)(A/C)GGTAACC, 0.

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

See also

References

  1. 1.0 1.1 1.2 Oldfield AJ, Yang P, Conway AE, Cinghu S, Freudenberg JM, Yellaboina S, Jothi R (September 2014). "Histone-fold domain protein NF-Y promotes chromatin accessibility for cell type-specific master transcription factors". Molecular Cell. 55 (5): 708–22. doi:10.1016/j.molcel.2014.07.005. PMID 25132174.
  2. "Entrez Gene: NFYA nuclear transcription factor Y, alpha".
  3. Li XY, Mattei MG, Zaleska-Rutczynska Z, Hooft van Huijsduijnen R, Figueroa F, Nadeau J, Benoist C, Mathis D (March 1992). "One subunit of the transcription factor NF-Y maps close to the major histocompatibility complex in murine and human chromosomes". Genomics. 11 (3): 630–4. doi:10.1016/0888-7543(91)90070-U. PMID 1774067.
  4. 4.0 4.1 4.2 Maity SN, de Crombrugghe B (June 1998). "Role of the CCAAT-binding protein CBF/NF-Y in transcription". Trends Biochemical Sciences. 23 (5): 174–8. doi:10.1016/S0968-0004(98)01201-8. PMID 9612081.
  5. 5.0 5.1 Sinha S, Maity SN, Seldin MF, de Crombrugghe B (February 1997). "Chromosomal assignment and tissue expression of CBF-C/NFY-C, the third subunit of the mammalian CCAAT-binding factor". Genomics. 37 (2): 260–3. doi:10.1006/geno.1996.0555. PMID 8921405.
  6. 6.0 6.1 Bellorini M, Zemzoumi K, Farina A, Berthelsen J, Piaggio G, Mantovani R (August 1997). "Cloning and expression of human NF-YC". Gene. 193 (1): 119–25. doi:10.1016/S0378-1119(97)00109-1. PMID 9249075.
  7. 7.0 7.1 7.2 "Entrez Gene: NFYC nuclear transcription factor Y, gamma".
  8. Oldfield AJ, Henriques T, Burkholder AB, Paulet D, Cinghu S, Yang P, Scruggs BS, Lavender CA, Kumar D, Bennett B, Rivals E (2019-07-11). "NF-Y controls fidelity of transcription initiation at gene promoters through maintenance of the nucleosome-depleted region". Nature Communications. 10 (1): 3072. doi:10.1038/s41467-019-10905-7. PMC 6624317 Check |pmc= value (help). PMID 31296853.
  9. "Entrez Gene: NFYB nuclear transcription factor Y, beta".
  10. Joglekar MV, Patil D, Joglekar VM, Rao GV, Reddy DN, Mitnala S, Shouche Y, Hardikar AA (September–October 2009). "The miR-30 family microRNAs confer epithelial phenotype to human pancreatic cells". Islets. 1 (2): 137–147. doi:10.4161/isl.1.2.9578. PMID 21099261.
  11. 11.0 11.1 Li XY, Mattei MG, Zaleska-Rutczynska Z, Hooft van Huijsduijnen R, Figueroa F, Nadeau J, Benoist C, Mathis D (November 1991). "One subunit of the transcription factor NF-Y maps close to the major histocompatibility complex in murine and human chromosomes". Genomics. 11 (3): 630–4. doi:10.1016/0888-7543(91)90070-U. PMID 1774067.
  12. 12.0 12.1 Yamada K, Osawa H, Granner DK (October 1999). "Identification of proteins that interact with NF-YA". FEBS Letters. 460 (1): 41–5. doi:10.1016/S0014-5793(99)01311-3. PMID 10571058.
  13. Yamada K, Printz RL, Osawa H, Granner DK (August 1999). "Human ZHX1: cloning, chromosomal location, and interaction with transcription factor NF-Y". Biochemical and Biophysical Research Communications. 261 (3): 614–21. doi:10.1006/bbrc.1999.1087. PMID 10441475.
  14. Imbriano C, Bolognese F, Gurtner A, Piaggio G, Mantovani R (July 2001). "HSP-CBF is an NF-Y-dependent coactivator of the heat shock promoters CCAAT boxes". J. Biol. Chem. 276 (28): 26332–9. doi:10.1074/jbc.M101553200. PMID 11306579.
  15. Pise-Masison CA, Dittmer J, Clemens KE, Brady JN (March 1997). "Physical and functional interaction between the human T-cell lymphotropic virus type 1 Tax1 protein and the CCAAT binding protein NF-Y". Mol. Cell. Biol. 17 (3): 1236–43. doi:10.1128/mcb.17.3.1236. PMC 231848. PMID 9032250.
  16. Izumi H, Molander C, Penn LZ, Ishisaki A, Kohno K, Funa K (April 2001). "Mechanism for the transcriptional repression by c-Myc on PDGF beta-receptor". J. Cell Sci. 114 (Pt 8): 1533–44. PMID 11282029.
  17. Bellorini M, Lee DK, Dantonel JC, Zemzoumi K, Roeder RG, Tora L, Mantovani R (June 1997). "CCAAT binding NF-Y-TBP interactions: NF-YB and NF-YC require short domains adjacent to their histone fold motifs for association with TBP basic residues". Nucleic Acids Research. 25 (11): 2174–81. doi:10.1093/nar/25.11.2174. PMC 146709. PMID 9153318.
  18. Taira T, Sawai M, Ikeda M, Tamai K, Iguchi-Ariga SM, Ariga H (August 1999). "Cell cycle-dependent switch of up-and down-regulation of human hsp70 gene expression by interaction between c-Myc and CBF/NF-Y". Journal Biol. Chem. 274 (34): 24270–9. doi:10.1074/jbc.274.34.24270. PMID 10446203.
  19. D. W. Yao, J. Luo, Q. Y. He, J. Li, H. Wang, H. B. Shi, H. F. Xu, M. Wang and J. J. Loor (May 2016). "Characterization of the liver X receptor-dependent regulatory mechanism of goat stearoyl-coenzyme A desaturase 1 gene by linoleic acid". Journal of Dairy Science. 99 (5): 3945–3957. doi:10.3168/jds.2015-10601. PMID 26947306. Retrieved 5 September 2020.

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