DAF-16-associated element gene transcriptions

Associate Editor(s)-in-Chief: Henry A. Hoff

"DAF-16 binding element (DBE), GTAAACA or TGTTTAC, and DAF-16-associated element (DAE), TGATAAG or CTTATCA, enriched in DAF-16 regulated genes [2, 4, 13, 14]. The DBE was recognized by DAF-16, and the DAE by transcription factor PQM-1 [13]."^[1] Note: GTAAACA is the inverse complement (ic) of TGTTTAC and TGATAAG is the ic of CTTATCA.

Consensus sequences

5'-TGATAAG-3'.^[1]

Hypotheses

A1BG has no DAF-16-associated elements in either promoter.
A1BG is not transcribed by a DAF-16-associated element.
DAF-16-associated element does not participate in the transcription of A1BG.

Samplings

Copying the consensus sequence 5'-TGATAAG-3' into ⌘F has found no elements in either A1BG promoter.

For the Basic programs testing consensus sequence 5'-TGATAAG-3' (starting with SuccessablesAAA.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

negative strand, negative direction, looking for 5'-TGATAAG-3', 0.
negative strand, positive direction, looking for 5'-TGATAAG-3', 0.
positive strand, negative direction, looking for 5'-TGATAAG-3', 0.
positive strand, positive direction, looking for 5'-TGATAAG-3', 0.
complement, negative strand, negative direction, looking for 5'-ACTATTC-3', 0.
complement, negative strand, positive direction, looking for 5'-ACTATTC-3', 0.
complement, positive strand, negative direction, looking for 5'-ACTATTC-3', 0.
complement, positive strand, positive direction, looking for 5'-ACTATTC-3', 0.
inverse complement, negative strand, negative direction, looking for 5'-CTTATCA-3', 0.
inverse complement, negative strand, positive direction, looking for 5'-CTTATCA-3', 0.
inverse complement, positive strand, negative direction, looking for 5'-CTTATCA-3', 0.
inverse complement, positive strand, positive direction, looking for 5'-CTTATCA-3', 0.
inverse negative strand, negative direction, looking for 5'-GAATAGT-3', 0.
inverse negative strand, positive direction, looking for 5'-GAATAGT-3', 0.
inverse positive strand, negative direction, looking for 5'-GAATAGT-3', 0.
inverse positive strand, positive direction, looking for 5'-GAATAGT-3', 0.

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

References

↑ ^1.0 ^1.1 Yan-Hui Li and Gai-Gai Zhang (12 April 2016). "Towards understanding the lifespan extension by reduced insulin signaling: bioinformatics analysis of DAF-16/FOXO direct targets in Caenorhabditis elegans". Oncotarget. 7 (15): 19185–19192. doi:10.18632/oncotarget.8313. PMID 2702736. Retrieved 27 August 2020.

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