Gal4p gene transcriptions

Associate Editor(s)-in-Chief: Henry A. Hoff

"P_GAL1 is often used repeatedly for the expression of different enzymes in the construction of metabolic pathways [13], so the gene copy number has to be increased to compensate for the insufficient promoter strength [8], and the utilization of too many galactose-inducible promoters may interfere with the metabolism of galactose due to the depletion of the transcription activator Gal4p [14]."^[1]

Galactose-inducible "promoters have various conserved UAS_GAL sites, a 17-bp consensus sequence 5′-CCGNNNNNNNNNNNCGG-3′ that is recognized by the transcription activator Gal4p to control their expression activities [38]."^[1]

Human genes

Interactions

"During the induction of galactose, the production of Gal4p can improve the PGAL1 activity 1000-fold [37]."^[1]

"We used the yeast Gal4p two-hybrid system (Fields and Sternglanz, 1994) to identify proteins that physically interact with GLD-1. We recovered two identical cDNAs in two-hybrid screens [...]. One (OG2.3) using GLD-1 residues 84-341 and the other (CD13.1) using residues 273-457, both fused to the Gal4p DNA binding domain [...]."^[2]

Consensus sequences

Upstream activating sequence for Gal4p is 5'-CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG-3' [38].^[1]

Hypotheses

A1BG has no Gal4ps in either promoter.
A1BG is not transcribed by a Gal4p.
Gal4p does not participate in the transcription of A1BG.

Samplings

Copying 5'-CGGACCGC-3' in "⌘F" yields none between ZSCAN22 and A1BG and none between ZNF497 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG (starting with SuccessablesGal4.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

negative strand, negative direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
negative strand, positive direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
positive strand, negative direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
positive strand, positive direction, looking for CGG(A/G)NN(A/G)C(C/T)N(C/T)NCNCCG, 0.
complement, negative strand, negative direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
complement, negative strand, positive direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
complement, positive strand, negative direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
complement, positive strand, positive direction, looking for GCC(C/T)NN(C/T)G(A/G)N(A/G)NGNGGC, 0.
inverse complement, negative strand, negative direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
inverse complement, negative strand, positive direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
inverse complement, positive strand, negative direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
inverse complement, positive strand, positive direction, looking for CGGNGN(A/G)N(A/G)G(C/T)NN(C/T)CCG, 0.
inverse negative strand, negative direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.
inverse negative strand, positive direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.
inverse positive strand, negative direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.
inverse positive strand, positive direction, looking for GCCNCN(C/T)N(C/T)C(A/G)NN(A/G)GGC, 0.

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

References

↑ ^1.0 ^1.1 ^1.2 ^1.3 Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check |pmid= value (help). Retrieved 18 September 2020.
↑ Robert Clifford, Min-Ho Lee, Sudhir Nayak, Mitsue Ohmachi, Flav Giorgini and Tim Schedl (December 2000). "FOG-2, a novel F-box containing protein, associates with the GLD-1 RNA binding protein and directs male sex determination in the C. elegans hermaphrodite germline" (PDF). Development. 127 (24): 5265–76. PMID 11076749. Retrieved 10 August 2020.

External links

[Tang-1] 1.0 ^1.1 ^1.2 ^1.3 Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check |pmid= value (help). Retrieved 18 September 2020.

[Clifford-2] Robert Clifford, Min-Ho Lee, Sudhir Nayak, Mitsue Ohmachi, Flav Giorgini and Tim Schedl (December 2000). "FOG-2, a novel F-box containing protein, associates with the GLD-1 RNA binding protein and directs male sex determination in the C. elegans hermaphrodite germline" (PDF). Development. 127 (24): 5265–76. PMID 11076749. Retrieved 10 August 2020.

[1]

[2]

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Hybrid C, T boxes Hypoxia-inducible factor gene transcriptions Hypoxia response elements I box gene transcriptions Initiator element gene transcriptions Initiator-like element gene transcriptions Inositol/choline-responsive elements Interaction gene transcriptions Interferon regulatory factors Inverse copy gene transcriptions Jasmonic acid-responsive element gene transcriptions K-boxes Kozak sequence gene transcriptions Kruppel-associated box gene transcriptions Krüppel-like factor gene transcriptions L box gene transcriptions Leu3 gene transcriptions M35 box gene transcriptions MADS box gene transcriptions Maf recognition element gene transcriptions M box gene transcriptions Mcm1 regulatory factor gene transcriptions Met31p box gene transcriptions Metal responsive element gene transcriptions Middle sporulation element gene transcriptions Mig1p gene transcriptions Model samplings Motif ten element gene transcriptions Msn2,4p gene transcriptions Musashi binding element gene transcriptions MYB recognition element gene transcriptions Myelocytomatosis transcription factor gene transcriptions Myocyte enhancer factor gene transcriptions N-boxes Ndt80p gene transcriptions Nuclear factor 1 Nuclear factor 𝜿B Nuclear factor gene transcriptions Nuclear factor of activated T cell gene transcriptions (NFAT) Nuclear factor Y gene transcriptions Nutrient-sensing response element gene transcriptions Oaf1p gene transcriptions ORE1 binding site gene transcriptions p53 response element gene transcriptions P63 DNA-binding site gene transcriptions P box gene transcriptions Pdr1,3p gene transcriptions Peroxisome proliferator hormone response element gene transcriptions Phosphate starvation-response transcription factor gene transcriptions Pollen1 element gene transcriptions Polycomb response element gene transcriptions Preinitiation complex Preinitiation complex gene transcriptions Pribnow box gene transcriptions Prolamin box gene transcriptions Promoter gene transcriptions Proximal promoter gene transcriptions Promoter occurrence gene transcriptions Pyrimidine box gene transcriptions Q element gene transcriptions Rap1 regulatory factor gene transcriptions Reb1 general regulatory factor gene transcriptions Retinoblastoma control element gene transcriptions Retinoic acid response element gene transcriptions Rgt1p gene transcriptions Rlm1p gene transcriptions RNA polymerase II gene transcriptions RNA polymerase II holoenzyme complex Root specific element gene transcriptions ROR-response element gene transcriptions Rox1p gene transcriptions Rpn4p gene transcriptions R response element gene transcriptions SARE gene transcriptions Seed-specific element gene transcriptions Serum response element gene transcriptions Servenius sequence gene transcriptions Shoot specific element gene transcriptions Sip4p gene transcriptions Smp1p gene transcriptions Sp1 gene transcriptions Spaceflight gene expressions Specificity protein gene transcriptions STAT gene transcriptions Ste12p gene transcriptions Sterol response element gene transcriptions Sucrose box gene transcriptions Synaptic Activity-Responsive Elements TACTAAC box gene transcriptions TAGteam gene transcriptions Tapetum box gene transcriptions TATA binding protein associated factor gene transcriptions TATA binding protein gene transcriptions TATA box gene transcriptions TAT box gene transcriptions TATC box gene transcriptions Tbf1 regulatory factor gene transcriptions T box gene transcriptions TCCACCATA element gene transcriptions TC element gene transcriptions TCT gene transcriptions TEA consensus sequence gene transcriptions Tec1p gene transcriptions Telomeric repeat DNA-binding factor gene transcriptions Tetradecanoylphorbol-13-acetate response element gene transcriptions TGF-β control elements (TCEs) TGF-β inhibitory elements (TIEs) Thyroid hormone response element gene transcriptions Transcriptional regulation Transcription bubble gene transcriptions Transcription factor gene transcriptions Transcription factor 3 gene transcriptions Transcription factory gene transcriptions Transcription start site gene transcriptions Translational control sequence gene transcriptions U box gene transcriptions Unfolded protein response element gene transcriptions Upstream response element gene transcriptions Upstream stimulatory factor gene transcriptions UTR promoter gene transcriptions V and P box gene transcriptions V box gene transcriptions Vhr1p gene transcriptions Vitamin D response element gene transcriptions W box gene transcriptions X box gene transcriptions Xbp1p gene transcriptions X core promoter element gene transcriptions Xenobiotic response element gene transcriptions Xenobiotic responsive element gene transcriptions Yap1p,2p gene transcriptions Y box gene transcriptions YY1 gene transcriptions Zap1p gene transcriptions Z box gene transcriptions Zinc responsive element gene transcriptions