Met31p box gene transcriptions

Associate Editor(s)-in-Chief: Henry A. Hoff

"The transcriptional regulation of the sulfur amino acid pathway in Saccharomyces cerevisiae depends on a single activator, Met4p, whose function requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p. The first description of how these factors cooperate to activate transcription was provided by the identification of the Cbf1–Met4–Met28 complex which is assembled on the regulatory region of the MET16 gene."^[1]

Human genes

Gene expressions

Interactions

Two "independent domains within Met4p are required for its tethering to the AAACTGTG sequence by the Met28p, Met31p and Met32p proteins."^[1]

The "bZIP domain of Met4p [is] necessary for the assembly of all the heteromeric complexes that have so far been demonstrated to allow the recruitment of Met4p to the promoter regions."^[1]

Consensus sequences

"Both Met31p and Met32p bind to the 5′‐AAACTGTG‐3′ core sequence which is, besides the 5′‐TCACGTG‐3′ element, the second regulatory element known to be involved in the regulation of several MET genes (Thomas et al., 1989)."^[1]

Binding site for

"Met28p does bind to the AAACTGTG sequence through its association with the high‐molecular‐weight complex."^[1]

Both "Met4p and Met28p are capable of binding to the AAACTGTG motif found upstream of the MET28 gene when associated with either Met31p or Met32p."^[1]

"The MET3 gene was chosen because its 5′ upstream region, like that of the MET28 gene, contains the AAACTGTG motif together with the TCACGTG sequence, the Cbf1p‐binding site (Thomas and Surdin‐Kerjan, 1997)."^[1]

Complement copies

Inverse copies

"A visual inspection revealed the presence of a AAACTGTG motif at position −145 (numbered relative to the ATG initiation codon) upstream of the MET28 gene. It must be noted that, in this case, the motif is found in the opposite orientation."^[1]

Complement-inverse copies

Enhancer activity

Promoter occurrences

Other "pathways are used to recruit Met4p on the 5′ upstream region of the two genes, MET3 and MET28. In these cases, Met4p is tethered to DNA through two alternative complexes associating Met4p with Met28p and either Met31p or Met32p. These complexes are formed over the AAACTGTG sequence, a cis‐acting element found upstream of several MET genes."^[1]

"The 5′ upstream regions of both MET3 and MET28 genes comprise the AAACTGTG motif together with the Cbf1p DNA‐binding site, TCACGTG."^[1]

Hypotheses

A1BG has no Met regulatory elements in either promoter.
A1BG is not transcribed by a Met regulatory element.
No Met regulatory element participates in the transcription of A1BG.

Samplings

Copying a responsive elements consensus sequence AAACTGTG and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence AAACTGTG (starting with SuccessablesMet.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

negative strand, negative direction, looking for AAAAAAAA, 0.
positive strand, negative direction, looking for AAAAAAAA, 0.
positive strand, positive direction, looking for AAAAAAAA, 0.
negative strand, positive direction, looking for AAAAAAAA, 0.
complement, negative strand, negative direction, looking for TTTTTTTT, 0.
complement, positive strand, negative direction, looking for TTTTTTTT, 0.
complement, positive strand, positive direction, looking for TTTTTTTT, 0.
complement, negative strand, positive direction, looking for TTTTTTTT, 0.
inverse complement, negative strand, negative direction, looking for TTTTTTTT, 0.
inverse complement, positive strand, negative direction, looking for TTTTTTTT, 0.
inverse complement, positive strand, positive direction, looking for TTTTTTTT, 0.
inverse complement, negative strand, positive direction, looking for TTTTTTTT, 0.
inverse negative strand, negative direction, looking for AAAAAAAA, 0.
inverse positive strand, negative direction, looking for AAAAAAAA, 0.
inverse positive strand, positive direction, looking for AAAAAAAA, 0.
inverse negative strand, positive direction, looking for AAAAAAAA, 0.

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

References

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Pierre‐Louis Blaiseau and Dominique Thomas (2 November 1998). "Multiple transcriptional activation complexes tether the yeast activator Met4 to DNA". The EMBO Journal. 17: 6327–6336. doi:10.1093/emboj/17.21.6327. |access-date= requires |url= (help)

External links

[Blaiseau-1] 1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Pierre‐Louis Blaiseau and Dominique Thomas (2 November 1998). "Multiple transcriptional activation complexes tether the yeast activator Met4 to DNA". The EMBO Journal. 17: 6327–6336. doi:10.1093/emboj/17.21.6327. |access-date= requires |url= (help)

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