K-box gene transcriptions

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Associate Editor(s)-in-Chief: Henry A. Hoff

"In fact, the groE genes were greatly induced upon heat shock in the hrcA mutant which was dark-treated prior to the heat shock in order to keep the background initial level of the groE mRNA accumulation as low as possible. These results led us to find a novel regulatory sequence which is involved in a heat and/or light mediated regulation of the groESL1 operon (Kojima and Nakamoto, 2007). This DNA element (GTTCGG-NNAN-CCNNAC) is located upstream of the groE promoters. It is highly conserved among the groESL1 operons from various cyanobacterial genomes. We designated this DNA element to be K-box. Removal of the K-box containing region upstream of the groESL1 promoter abolished heat- and/or light-induced transcription of the operon completely. K-box is also present upstream of dnaK2, one of the three dnaK genes in cyanobacteria (Kojima and Nakamoto, 2007; Sato et al., 2007)."[1]

K-box samplings

Copying a responsive elements consensus sequence GTTCGG-NNAN-CCNNAC and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence GTTCGGNNANCCNNAC (starting with SuccessablesK-box.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

  1. negative strand, negative direction, looking for GTTCGGNNANCCNNAC, 0.
  2. positive strand, negative direction, looking for GTTCGGNNANCCNNAC, 0.
  3. positive strand, positive direction, looking for GTTCGGNNANCCNNAC, 0.
  4. negative strand, positive direction, looking for GTTCGGNNANCCNNAC, 0.
  5. complement, negative strand, negative direction, looking for CAAGCCNNTNGGNNTG, 0.
  6. complement, positive strand, negative direction, looking for CAAGCCNNTNGGNNTG, 0.
  7. complement, positive strand, positive direction, looking for CAAGCCNNTNGGNNTG, 0.
  8. complement, negative strand, positive direction, looking for CAAGCCNNTNGGNNTG, 0.
  9. inverse complement, negative strand, negative direction, looking for GTNNGGNTNNCCGAAC, 0.
  10. inverse complement, positive strand, negative direction, looking for GTNNGGNTNNCCGAAC, 0.
  11. inverse complement, positive strand, positive direction, looking for GTNNGGNTNNCCGAAC, 0.
  12. inverse complement, negative strand, positive direction, looking for GTNNGGNTNNCCGAAC, 0.
  13. inverse negative strand, negative direction, looking for CANNCCNANNGGCTTG, 0.
  14. inverse positive strand, negative direction, looking for CANNCCNANNGGCTTG, 0.
  15. inverse positive strand, positive direction, looking for CANNCCNANNGGCTTG, 0.
  16. inverse negative strand, positive direction, looking for CANNCCNANNGGCTTG, 0.

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

See also

References

  1. Masakazu Saito, Satoru Watanabe, Kaori Nimura-Matsune, Hirofumi Yoshikawa1, 8 Hitoshi Nakamoto (10 April 2020). "Regulation of the groESL1 transcription by the HrcA repressor and a novel transcription factor Orf7.5 in the cyanobacterium Synechococcus elongatus PCC7942". The Journal of General and Applied Microbiology. 66 (2): 85–92. doi:10.2323/jgam.2020.02.001. Retrieved 17 March 2021. line feed character in |author= at position 76 (help)

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