G box gene transcriptions: Difference between revisions

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(G/T)CCACGTG(G/T)C combines the PA G-box (GCCACGTGGC) with the G-box of ''GmAux28'' (TCCACGTGTC) for testing both.
(G/T)CCACGTG(G/T)C combines the PA G-box (GCCACGTGGC) with the G-box of ''GmAux28'' (TCCACGTGTC) for testing both.


==Samplings==
==Oeda samplings==


For the Basic programs (starting with SuccessablesGbox.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), including extending the number of nts from 958 to 4445, the programs are, are looking for, and found:
For the Basic programs (starting with SuccessablesGbox.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), including extending the number of nts from 958 to 4445, the programs are, are looking for, and found:

Revision as of 17:35, 16 July 2021

Editor-In-Chief: Henry A. Hoff

"Two distinct sequence elements, the H-box (consensus CCTACC(N)7CT) and the G-box (CACGTG), are required for stimulation of the chsl5 promoter by [p-coumaric acid] 4-CA."[1]

G boxes

The "perfect palindrome 5'-GCCACGTGGC-3' which is also known as the G-box motif."[2]

"TAF-1 can bind to the G-box and related motifs and that it functions as a transcription activator."[2]

"A G-box-related motif, containing the core sequence CACGTG is also present in the 5' regions of two other classes of light-responsive genes".[2]

Most bZIP proteins show high binding affinity for the ACGT motifs, which include CACGTG (G box) [...].[3][4][5]

"Binding to the palindromic G-box (PA G-box, GCCACGTGGC) was moderate. However, binding activity to the G-box of the light-responsive unit 1 (U1) region of the parsley (Petroselinum crispum) CHS promoter (CHS-U1: TCCACGTGGC; Schulze-Lefert et al., 1989) or the G-box of GmAux28 (TCCACGTGTC) was much weaker than to the PA G-box [...]."[6]

(G/T)CCACGTG(G/T)C combines the PA G-box (GCCACGTGGC) with the G-box of GmAux28 (TCCACGTGTC) for testing both.

Oeda samplings

For the Basic programs (starting with SuccessablesGbox.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), including extending the number of nts from 958 to 4445, the programs are, are looking for, and found:

  1. negative strand in the negative direction (from ZSCAN22 to A1BG) is SuccessablesGbox--.bas, looking for 3'-GCCACGTGGC-5', 0,
  2. negative strand in the positive direction (from ZNF497 to A1BG) is SuccessablesGbox-+.bas, looking for 3'-GCCACGTGGC-5', 0,
  3. positive strand in the negative direction is SuccessablesGbox+-.bas, looking for 3'-GCCACGTGGC-5', 0,
  4. positive strand in the positive direction is SuccessablesGbox++.bas, looking for 3'-GCCACGTGGC-5', 0,
  5. complement, negative strand, negative direction is SuccessablesGboxc--.bas, looking for 3'-CGGTGCACCG-5', 0,
  6. complement, negative strand, positive direction is SuccessablesGboxc-+.bas, looking for 3'-CGGTGCACCG-5', 0,
  7. complement, positive strand, negative direction is SuccessablesGboxc+-.bas, looking for 3'-CGGTGCACCG-5', 0,
  8. complement, positive strand, positive direction is SuccessablesGboxc++.bas, looking for 3'-CGGTGCACCG-5', 0,
  9. inverse complement, negative strand, negative direction is SuccessablesGboxci--.bas, looking for 3'-GCCACGTGGC-5', 0,
  10. inverse complement, negative strand, positive direction is SuccessablesGboxci-+.bas, looking for 3'-GCCACGTGGC-5', 0,
  11. inverse complement, positive strand, negative direction is SuccessablesGboxci+-.bas, looking for 3'-GCCACGTGGC-5', 0,
  12. inverse complement, positive strand, positive direction is SuccessablesGboxci++.bas, looking for 3'-GCCACGTGGC-5', 0,
  13. inverse, negative strand, negative direction, is SuccessablesGboxi--.bas, looking for 3'-CGGTGCACCG-5', 0,
  14. inverse, negative strand, positive direction, is SuccessablesGboxi-+.bas, looking for 3'-CGGTGCACCG-5', 0,
  15. inverse, positive strand, negative direction, is SuccessablesGboxi+-.bas, looking for 3'-CGGTGCACCG-5', 0,
  16. inverse, positive strand, positive direction, is SuccessablesGboxi++.bas, looking for 3'-CGGTGCACCG-5', 0.

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

Initial content for this page in some instances came from Wikiversity.

See also

References

  1. Gary J. Loake, Ouriel Faktor, Christopher J. Lamb, and Richard A. Dixon (October 1992). "Combination of H-box [CCTACC(N)7CT] and G-box (CACGTG) cis elements is necessary for feed-forward stimulation of a chalcone synthase promoter by the phenylpropanoid-pathway intermediate p-coumaricacid" (PDF). Proceedings of the National Academy of Sciences USA. 89: 9230–4. Retrieved 5 May 2020.
  2. 2.0 2.1 2.2 K Oeda, J Salinas, and N H Chua (July 1991). "A tobacco bZip transcription activator (TAF-1) binds to a G-box-like motif conserved in plant genes". The EMBO Journal. 10 (7): 1793–1802. PMID 2050116. Retrieved 2017-02-13.
  3. Landschulz WH, Johnson PF, McKnight SL (June 1988). "The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins". Science. 240 (4860): 1759–64. Bibcode:1988Sci...240.1759L. doi:10.1126/science.3289117. PMID 3289117.
  4. Z G E, Zhang YP, Zhou JH, Wang L (April 2014). "Mini review roles of the bZIP gene family in rice". Genetics and Molecular Research. 13 (2): 3025–36. doi:10.4238/2014.April.16.11. PMID 24782137. Vancouver style error: name (help)
  5. Nijhawan A, Jain M, Tyagi AK, Khurana JP (February 2008). "Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice". Plant Physiology. 146 (2): 333–50. doi:10.1104/pp.107.112821. PMID 18065552.
  6. Young Hun Song, Cheol Min Yoo, An Pio Hong, Seong Hee Kim, Hee Jeong Jeong, Su Young Shin, Hye Jin Kim, Dae-Jin Yun, Chae Oh Lim, Jeong Dong Bahk, Sang Yeol Lee, Ron T. Nagao, Joe L. Key, and Jong Chan Hong (April 2008). "DNA-Binding Study Identifies C-Box and Hybrid C/G-Box or C/A-Box Motifs as High-Affinity Binding Sites for STF1 and LONG HYPOCOTYL5 Proteins" (PDF). Plant Physiology. 146 (4): 1862–1877. doi:10.1104/pp.107.113217. PMID 18287490. Retrieved 26 March 2019.

External links

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