DREB box gene transcriptions: Difference between revisions

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(Created page with "'''Editor-In-Chief:''' Henry A. Hoff It "is likely that the present OsDREBL may preferably bind to other elements such as the ethylene responsive element GCC box (AGCCGCC), r...")
 
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Increasing the number of nts from A1BG to ZNF497 from 958 to 4445 has yielded no DREB boxes.
Increasing the number of nts from A1BG to ZNF497 from 958 to 4445 has yielded no DREB boxes.
==Acknowledgements==
The content on this page was first contributed by: Henry A. Hoff.
Initial content for this page in some instances came from [http://www.wikiversity.org Wikiversity].


==See also==
==See also==

Revision as of 02:40, 16 October 2019

Editor-In-Chief: Henry A. Hoff

It "is likely that the present OsDREBL may preferably bind to other elements such as the ethylene responsive element GCC box (AGCCGCC), rather than the CRT/DREB box (TACCGACAT), since the two boxes were very similar."[1]

"Based on DNA-binding data, this group [one AP2/ERF domain only] has been subdivided into dehydration-responsive element-binding (DREB)-like proteins, which interact with the dehydration-responsive, or cold-repeat, element (consensus sequence TACCGACAT) (Liu et al. 1998; Stockinger et al. 1997) and the EREBP-like (ethylene responsive element binding protein) proteins binding the GCC box (consensus sequence AGCCGCC) (Ohme-Takagi and Shinshi 1995; Sakuma et al. 2002)."[2]

DREB box sampling of A1BG promoters

Consensus sequence for the dehydration-responsive element-binding (DREB) box is 3'-TACCGACAT-5'.

For the Basic programs (starting with SuccessablesDREB.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

  1. negative strand in the negative direction (from ZSCAN22 to A1BG) is SuccessablesDREB--.bas, looking for 3'-TACCGACAT-5', 0,
  2. negative strand in the positive direction (from ZNF497 to A1BG) is SuccessablesDREB-+.bas, looking for 3'-TACCGACAT-5', 0,
  3. positive strand in the negative direction is SuccessablesDREB+-.bas, looking for 3'-TACCGACAT-5', 0,
  4. positive strand in the positive direction is SuccessablesDREB++.bas, looking for 3'-TACCGACAT-5', 0,
  5. complement, negative strand, negative direction is SuccessablesDREBc--.bas, looking for 3'-ATGGCTGTA-5', 0,
  6. complement, negative strand, positive direction is SuccessablesDREBc-+.bas, looking for 3'-ATGGCTGTA-5', 0,
  7. complement, positive strand, negative direction is SuccessablesDREBc+-.bas, looking for 3'-ATGGCTGTA-5', 0,
  8. complement, positive strand, positive direction is SuccessablesDREBc++.bas, looking for 3'-ATGGCTGTA-5', 0,
  9. inverse complement, negative strand, negative direction is SuccessablesDREBci--.bas, looking for 3'-ATGTCGGTA-5', 0,
  10. inverse complement, negative strand, positive direction is SuccessablesDREBci-+.bas, looking for 3'-ATGTCGGTA-5', 0,
  11. inverse complement, positive strand, negative direction is SuccessablesDREBci+-.bas, looking for 3'-ATGTCGGTA-5', 0,
  12. inverse complement, positive strand, positive direction is SuccessablesDREBci++.bas, looking for 3'-ATGTCGGTA-5', 0,
  13. inverse, negative strand, negative direction, is SuccessablesDREBi--.bas, looking for 3'-TACAGCCAT-5', 0,
  14. inverse, negative strand, positive direction, is SuccessablesDREBi-+.bas, looking for 3'-TACAGCCAT-5', 0,
  15. inverse, positive strand, negative direction, is SuccessablesDREBi+-.bas, looking for 3'-TACAGCCAT-5', 0,
  16. inverse, positive strand, positive direction, is SuccessablesDREBi++.bas, looking for 3'-TACAGCCAT-5', 0.

Increasing the number of nts from A1BG to ZNF497 from 958 to 4445 has yielded no DREB boxes.

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

Initial content for this page in some instances came from Wikiversity.

See also

References

  1. Jian-Quan Chen, Yi Dong, Yu-Jun Wang, Qiang Liu, Jin-Song Zhang, Shou-Yi Chen (2003). "An AP2/EREBP-type transcription-factor gene from rice is cold-inducible and encodes a nuclear-localized protein". Theoretical and Applied Genetics. 107 (6): 972–979. doi:10.1007/s00122-003-1346-5. Retrieved 2017-05-30. Unknown parameter |month= ignored (help)
  2. Ute Fischer and Wolfgang Dröge-Laser (2004). "Overexpression of NtERF5, a New Member of the Tobacco Ethylene Response Transcription Factor Family Enhances Resistance to Tobacco mosaic virus". Molecular Plant-Microbe Interactions. 17 (10): 1162–1171. doi:10.1094/MPMI.2004.17.10.1162. Retrieved 2017-05-30.

External links

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