Endosperm expression gene transcriptions: Difference between revisions

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==Response element analysis and results==
==Response element analysis and results==
{{main|Complex locus A1BG and ZNF497#Endosperm expressions}}
{{main|Complex locus A1BG and ZNF497#Endosperm expressions}}
Endosperm expression (TGTGTCA).<ref name=Sharma/>
{|class="wikitable"
|-
! Reals or randoms !! Promoters !! direction !! Numbers !! Strands !! Occurrences !! Averages (± 0.1)
|-
| Reals || UTR || negative || 1 || 2 || 0.5 || 0.5
|-
| Randoms || UTR || arbitrary negative || 1 || 10 || 0.1 || 0.05
|-
| Randoms || UTR || alternate negative || 0 || 10 || 0 || 0.05
|-
| Reals || Core || negative || 0 || 2 || 0 || 0
|-
| Randoms || Core || negative || 0 || 10 || 0 || 0
|-
| Reals || Core || positive || 0 || 2 || 0 || 0
|-
| Randoms || Core || positive || 0 || 10 || 0 || 0
|-
| Reals || Proximal || negative || 0 || 2 || 0 || 0
|-
| Randoms || Proximal || negative || 0 || 10 || 0 || 0
|-
| Reals || Proximal || positive || 0 || 2 || 0 || 0
|-
| Randoms || Proximal || positive || 0 || 10 || 0 || 0
|-
| Reals || Distal || negative || 0 || 2 || 0 || 0
|-
| Randoms || Distal || negative || 0 || 10 || 0 || 0.05
|-
| Reals || Distal || positive || 2 || 2 || 1.0 || 0.5
|-
| Randoms || Distal || positive || 1 || 10 || 0.1 || 0.05
|}
Comparison:
The occurrences of real endosperm expression consensus sequences are larger than the randoms. This suggests that the endosperm expression consensus sequences are likely active or activable.


==Acknowledgements==
==Acknowledgements==

Revision as of 23:21, 10 April 2022

Associate Editor(s)-in-Chief: Henry A. Hoff

Human genes

Gene expressions

Consensus sequences

Endosperm expression (TGTGTCA).[1]

Hypotheses

  1. A1BG has no Endosperm expression in either promoter.
  2. A1BG is not transcribed by Endosperm expression.
  3. Endosperm expression does not participate in the transcription of A1BG.

Samplings

Copying an apparent consensus sequence of TGTGTCA and putting it in "⌘F" finds one located between ZSCAN22 and A1BG and none between ZNF497 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence 5'-TGTGTCA-3' (starting with SuccessablesEE.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

  1. negative strand, negative direction, looking for 5'-TGTGTCA-3', 1, 5'-TGTGTCA-3' at 4198.
  2. negative strand, positive direction, looking for 5'-TGTGTCA-3', 0.
  3. positive strand, negative direction, looking for 5'-TGTGTCA-3', 0.
  4. positive strand, positive direction, looking for 5'-TGTGTCA-3', 1, 5'-TGTGTCA-3' at 3413.
  5. complement, negative strand, negative direction, looking for 5'-ACACAGT-3', 0.
  6. complement, negative strand, positive direction, looking for 5'-ACACAGT-3', 1, 5'-ACACAGT-3' at 3413.
  7. complement, positive strand, negative direction, looking for 5'-ACACAGT-3', 1, 5'-ACACAGT-3' at 4198.
  8. complement, positive strand, positive direction, looking for 5'-ACACAGT-3', 0.
  9. inverse complement, negative strand, negative direction, looking for 5'-TGACACA-3', 0.
  10. inverse complement, negative strand, positive direction, looking for 5'-TGACACA-3', 0.
  11. inverse complement, positive strand, negative direction, looking for 5'-TGACACA-3', 0.
  12. inverse complement, positive strand, positive direction, looking for 5'-TGACACA-3', 1, 5'-TGACACA-3' at 2076.
  13. inverse negative strand, negative direction, looking for 5'-ACTGTGT-3', 0.
  14. inverse negative strand, positive direction, looking for 5'-ACTGTGT-3', 1, 5'-ACTGTGT-3' at 2076.
  15. inverse positive strand, negative direction, looking for 5'-ACTGTGT-3', 0.
  16. inverse positive strand, positive direction, looking for 5'-ACTGTGT-3', 0.

EE UTR

Negative strand, negative direction: TGTGTCA at 4198.

EE distal promoters

Negative strand, positive direction: TGACACA at 2076.

Positive strand, positive direction: TGTGTCA at 3413.

Endosperm expression random dataset samplings

  1. EEr0: 1, TGTGTCA at 3959.
  2. EEr1: 0.
  3. EEr2: 0.
  4. EEr3: 0.
  5. EEr4: 0.
  6. EEr5: 1, TGTGTCA at 2839.
  7. EEr6: 0.
  8. EEr7: 0.
  9. EEr8: 0.
  10. EEr9: 0.
  11. EEr0ci: 0.
  12. EEr1ci: 0.
  13. EEr2ci: 0.
  14. EEr3ci: 0.
  15. EEr4ci: 0.
  16. EEr5ci: 0.
  17. EEr6ci: 0.
  18. EEr7ci: 0.
  19. EEr8ci: 0.
  20. EEr9ci: 0.

EEr UTRs

  1. EEr0: TGTGTCA at 3959.

EEr distal promoters

  1. EEr5: TGTGTCA at 2839.

Response element analysis and results

Endosperm expression (TGTGTCA).[1]

Reals or randoms Promoters direction Numbers Strands Occurrences Averages (± 0.1)
Reals UTR negative 1 2 0.5 0.5
Randoms UTR arbitrary negative 1 10 0.1 0.05
Randoms UTR alternate negative 0 10 0 0.05
Reals Core negative 0 2 0 0
Randoms Core negative 0 10 0 0
Reals Core positive 0 2 0 0
Randoms Core positive 0 10 0 0
Reals Proximal negative 0 2 0 0
Randoms Proximal negative 0 10 0 0
Reals Proximal positive 0 2 0 0
Randoms Proximal positive 0 10 0 0
Reals Distal negative 0 2 0 0
Randoms Distal negative 0 10 0 0.05
Reals Distal positive 2 2 1.0 0.5
Randoms Distal positive 1 10 0.1 0.05

Comparison:

The occurrences of real endosperm expression consensus sequences are larger than the randoms. This suggests that the endosperm expression consensus sequences are likely active or activable.

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

See also

References

  1. 1.0 1.1 Bhaskar Sharma; Joemar Taganna (12 June 2020). "Genome-wide analysis of the U-box E3 ubiquitin ligase enzyme gene family in tomato". Scientific Reports. 10 (9581). doi:10.1038/s41598-020-66553-1. PMID 32533036 Check |pmid= value (help). Retrieved 27 August 2020.

External links