L box gene transcriptions: Difference between revisions

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==Samplings==
==Samplings==


Copying the above consensus L box sequence and putting the sequences or portions of it in "⌘F" should locate these in some nucleotide positions unless cutoff by the Carriage return, line feed of the applied "Return" after so many nucleotides, as should be found by the computer programs. If none matching the consensus sequence is found even including checking the line ends, it is unlikely that even one L box is within either A1BG promoter.
Copying a responsive elements consensus sequence AAATTAACCAA and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.
 
For the Basic programs testing consensus sequence AAATTAACCAA (starting with SuccessablesLbox.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
# negative strand, negative direction, looking for AAATTAACCAA, 0.
# positive strand, negative direction, looking for AAATTAACCAA, 0.
# positive strand, positive direction, looking for AAATTAACCAA, 0.
# negative strand, positive direction, looking for AAATTAACCAA, 0.
# complement, negative strand, negative direction, looking for TTTTTTTT, 0.
# complement, positive strand, negative direction, looking for TTTTTTTT, 0.
# complement, positive strand, positive direction, looking for TTTTTTTT, 0.
# complement, negative strand, positive direction, looking for TTTTTTTT, 0.
# inverse complement, negative strand, negative direction, looking for TTTTTTTT, 0.
# inverse complement, positive strand, negative direction, looking for TTTTTTTT, 0.
# inverse complement, positive strand, positive direction, looking for TTTTTTTT, 0.
# inverse complement, negative strand, positive direction, looking for TTTTTTTT, 0.
# inverse negative strand, negative direction, looking for AACCAATTAAA, 0.
# inverse positive strand, negative direction, looking for AACCAATTAAA, 0.
# inverse positive strand, positive direction, looking for AACCAATTAAA, 0.
# inverse negative strand, positive direction, looking for AACCAATTAAA, 0.


==See also==
==See also==
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{{div col|colwidth=20em}}
* [[A1BG gene transcriptions]]
* [[A1BG gene transcriptions]]
* [[G box gene transcriptions]]
* [[A1BG regulatory elements and regions]]
* [[I box gene transcriptions]]
* [[A1BG response element positive results]]
* [[Complex locus A1BG and ZNF497]]
{{Div col end}}
{{Div col end}}


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==External links==
==External links==
* [http://www.genome.jp/ GenomeNet KEGG database]
* [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene Home - Gene - NCBI]
* [http://www.ncbi.nlm.nih.gov/sites/gquery NCBI All Databases Search]
* [http://www.ncbi.nlm.nih.gov/ncbisearch/ NCBI Site Search]


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Revision as of 03:39, 2 February 2021

File:Lobelia nicotianifolia-2-yercaud-salem-India.jpg
An erect herb is photographed Nicotiana plumbaginifolia which will have white flowers. Credit: Yercaud-elango.{{free media}}

"Sequence comparison of the tomato RbcS-3A and RbcS-1 upstream regions shows three regions of strong homology, named the L [5'-AAATTAACCAAC-3'], I, and G boxes [...], contained within the fragment showing affinity for GBF. These homologies are highly significant, since these two genes diverged a long time ago (3) and do not share, in their upstream sequences, extensive regions of homology. All three boxes are found in tobacco (24) (Nicotiana tabacum) and Nicotiana plumbaginifolia (25) RBCS upstream sequences [...], while soybean upstream sequences lack a bona fide L box sequence (26)."[1]

"In the tomato RbcS-3A promoter, a near-perfect box II homology (CATTTTCACT) is found just downstream of the L box [...]."[1]

Consensus sequences

"The sequence targeted by substitution P4 overlaps a sequence showing homology to the 'L' box (consensus 5'-AAATTAACCAA-3'), which is conserved in RBCS upstream sequences of both tomato and tobacco (Giuliano et al., 1988)."[2]

Hypotheses

  1. A1BG promoters do not contain an L box.

Samplings

Copying a responsive elements consensus sequence AAATTAACCAA and putting the sequence in "⌘F" finds none between ZNF497 and A1BG or none between ZSCAN22 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence AAATTAACCAA (starting with SuccessablesLbox.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

  1. negative strand, negative direction, looking for AAATTAACCAA, 0.
  2. positive strand, negative direction, looking for AAATTAACCAA, 0.
  3. positive strand, positive direction, looking for AAATTAACCAA, 0.
  4. negative strand, positive direction, looking for AAATTAACCAA, 0.
  5. complement, negative strand, negative direction, looking for TTTTTTTT, 0.
  6. complement, positive strand, negative direction, looking for TTTTTTTT, 0.
  7. complement, positive strand, positive direction, looking for TTTTTTTT, 0.
  8. complement, negative strand, positive direction, looking for TTTTTTTT, 0.
  9. inverse complement, negative strand, negative direction, looking for TTTTTTTT, 0.
  10. inverse complement, positive strand, negative direction, looking for TTTTTTTT, 0.
  11. inverse complement, positive strand, positive direction, looking for TTTTTTTT, 0.
  12. inverse complement, negative strand, positive direction, looking for TTTTTTTT, 0.
  13. inverse negative strand, negative direction, looking for AACCAATTAAA, 0.
  14. inverse positive strand, negative direction, looking for AACCAATTAAA, 0.
  15. inverse positive strand, positive direction, looking for AACCAATTAAA, 0.
  16. inverse negative strand, positive direction, looking for AACCAATTAAA, 0.

See also

References

  1. 1.0 1.1 G. Giuliano, E. Pichersky, V. S. Malik, M. P. Timko, P. A. Scolniks, and A. R. Cashmore (1 October 1988). "An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene" (PDF). Proceedings of the National Academy of Sciences USA. 85 (19): 7089–93. doi:10.1073/pnas.85.19.7089. Retrieved 9 November 2018.
  2. Robert G. K. Donald and Anthony R. Cashmore (1990). "Mutation of either G box or I box sequences profoundly affects expression from the Arabidopsis rbcS‐1A promoter". The EMBO Journal. 9 (6): 1717–1726. doi:10.1002/j.1460-2075.1990.tb08295.x. Retrieved 8 November 2018.

External links