Oaf1p gene transcriptions: Difference between revisions

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{{AE}} Henry A. Hoff
{{AE}} Henry A. Hoff
"In ''S. cerevisiae'', peroxisomal proteins are repressed by glucose and induced by oleate (361). It has been shown that derepression of such proteins is dependent on Adr1 and on the Snf1 complex (320–322). Induction by oleate depends on a “peroxisome box” (110), also called the oleate response element (92), which is an imperfect palindrome including the consensus sequence CGGNNNTNA. There is evidence that the transcription factor binding this sequence is a heterodimer containing the proteins Oaf1 and Oaf2/Pip2, which are both C<sub>6</sub> zinc cluster proteins (165, 201, 293)."<ref name=Gancedo>{{ cite journal
|author=Juana M. Gancedo
|title=Yeast Carbon Catabolite Repression
|journal=Microbiology and Molecular Biology Reviews
|date=June 1998
|volume=62
|issue=2
|pages=334–361
|url=https://www.ncbi.nlm.nih.gov/pmc/articles/PMC98918/
|arxiv=
|bibcode=
|doi=
|pmid=9618445
|accessdate=6 February 2021 }}</ref>


==Human genes==
==Human genes==

Revision as of 01:31, 11 February 2021

Associate Editor(s)-in-Chief: Henry A. Hoff

"In S. cerevisiae, peroxisomal proteins are repressed by glucose and induced by oleate (361). It has been shown that derepression of such proteins is dependent on Adr1 and on the Snf1 complex (320–322). Induction by oleate depends on a “peroxisome box” (110), also called the oleate response element (92), which is an imperfect palindrome including the consensus sequence CGGNNNTNA. There is evidence that the transcription factor binding this sequence is a heterodimer containing the proteins Oaf1 and Oaf2/Pip2, which are both C6 zinc cluster proteins (165, 201, 293)."[1]

Human genes

Interactions

Consensus sequences

The upstream activating sequence (UAS) for the Oaf1p transcription factor is 5'-CGGN3TNAN9-12CCG-3' or 5'-CGG(A/C/G/T)(A/C/G/T)(A/C/G/T)T(A/C/G/T)A(A/C/G/T)9-12CCG-3'.[2]

Samplings

Copying CGGN3TNAN9-12CCG in "⌘F" yields none between ZSCAN22 and A1BG and none between ZNF497 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence CGGN3TNAN9-12CCG (starting with SuccessablesOaf1.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

  1. negative strand, negative direction, looking for CGGN3TNAN12CCG, 1, CGGTCATGAAACCCTCCGACTCCG at 2229.
  2. positive strand, negative direction, looking for CGGN3TNAN9-12CCG, 0.
  3. positive strand, positive direction, looking for CGGN3TNAN9CCG, 2, CGGACGTGAGACCGCTCTCCG at 1484, CGGACGTGAGACCGCTCTCCG at 1384.
  4. negative strand, positive direction, looking for CGGN3TNAN9-12CCG, 0.
  5. complement, negative strand, negative direction, looking for GCCN3ANTN9-12GGC, 0.
  6. complement, positive strand, negative direction, looking for GCCN3ANTN12GGC, 1, GCCAGTACTTTGGGAGGCTGAGGC at 2229.
  7. complement, positive strand, positive direction, looking for GCCN3ANTN9-12GGC, 0.
  8. complement, negative strand, positive direction, looking for GCCN3ANTN9GGC, 2, GCCTGCACTCTGGCGAGAGGC at 1484, GCCTGCACTCTGGCGAGAGGC at 1384.
  9. inverse complement, negative strand, negative direction, looking for CGGN9-12TNAN3CCG, 0.
  10. inverse complement, positive strand, negative direction, looking for CGGN9-12TNAN3CCG, 0.
  11. inverse complement, positive strand, positive direction, looking for CGGN9-12TNAN3CCG, 0.
  12. inverse complement, negative strand, positive direction, looking for CGGN9-12TNAN3CCG, 0.
  13. inverse negative strand, negative direction, looking for GCCN9-12ANTN3GGC, 0.
  14. inverse positive strand, negative direction, looking for GCCN9-12ANTN3GGC, 0.
  15. inverse positive strand, positive direction, looking for GCCN9-12ANTN3GGC, 0.
  16. inverse negative strand, positive direction, looking for GCCN9-12ANTN3GGC, 0.

Oaf1 distal promoters

Negative strand, negative direction: CGGTCATGAAACCCTCCGACTCCG at 2229.

Positive strand, positive direction: CGGACGTGAGACCGCTCTCCG at 1484, CGGACGTGAGACCGCTCTCCG at 1384.

See also

References

  1. Juana M. Gancedo (June 1998). "Yeast Carbon Catabolite Repression". Microbiology and Molecular Biology Reviews. 62 (2): 334–361. PMID 9618445. Retrieved 6 February 2021.
  2. Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check |pmid= value (help). Retrieved 18 September 2020.

External links