Hac1p gene transcriptions: Difference between revisions

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{{AE}} Henry A. Hoff
{{AE}} Henry A. Hoff
"The [unfolded protein response] UPR has been shown to play important roles in overexpression and secretion of some recombinant proteins [9]. Manipulating the level of transcription factors and [endoplasmic reticulum] ER chaperones in the UPR pathway improved secretion of recombinant proteins [10, 11]. An important characteristic of the UPR is that it is directly linked to transcription activation processes governed by ER stress conditions. In ''Sachcharomyces cerevisiae'' this process is mediated by a unique mechanism involving cooperative action of Hac1 transcription factor (TF) and a short conserved DNA sequence referred to as unfolded protein response element (UPRE) in the promoter of UPR target genes [12]. The UPRE regulatory ''cis'' acting element lies within 22-bp upstream of the promoter of UPR responsive genes, which is crucial for transcriptional induction under ER stress [13]. The best characterized UPRE core sequence was UPRE-1 (CANCNTG) from ''S. cerevisiae'' (for examples from KAR2, CAGCGTG and ''PDI1'', CACCGTG) [13, 14]. The similar UPRE-1 is also found in the promoter region of the ''P. pastoris'' ''KAR2'' (CAGCGTG), ''INO1'' (CAACTTG) and ''HAC1'' (CAACTTG) genes [15]. The presence of an ''HAC1'' UPRE implies that Hac1p can up-regulate its own transcription. Unconventional splicing of ''HAC1'' mRNA after ER stress signaling generates the active form of basic leucine zipper (bZIP) transcription factor Hac1p, which binds to the UPRE [16]."<ref name=Kullawong>{{ cite journal
|author=Niwed Kullawong, Sutipa Tanapongpipat, Lily Eurwilaichitr and Witoon Tirasophon
|title=Unfolded Protein Response (UPR) Induced Hybrid Promoters for Heterologous Gene Expression in ''Pichia pastoris''
|journal=Chiang Mai Journal of Science
|date=2018
|volume=45
|issue=7
|pages=2554-2565
|url=http://www.thaiscience.info/Journals/Article/CMJS/10990408.pdf
|arxiv=
|bibcode=
|doi=
|pmid=
|accessdate=11 January 2021 }}</ref>


==Human genes==
==Human genes==

Revision as of 02:39, 12 January 2021

Associate Editor(s)-in-Chief: Henry A. Hoff

"The [unfolded protein response] UPR has been shown to play important roles in overexpression and secretion of some recombinant proteins [9]. Manipulating the level of transcription factors and [endoplasmic reticulum] ER chaperones in the UPR pathway improved secretion of recombinant proteins [10, 11]. An important characteristic of the UPR is that it is directly linked to transcription activation processes governed by ER stress conditions. In Sachcharomyces cerevisiae this process is mediated by a unique mechanism involving cooperative action of Hac1 transcription factor (TF) and a short conserved DNA sequence referred to as unfolded protein response element (UPRE) in the promoter of UPR target genes [12]. The UPRE regulatory cis acting element lies within 22-bp upstream of the promoter of UPR responsive genes, which is crucial for transcriptional induction under ER stress [13]. The best characterized UPRE core sequence was UPRE-1 (CANCNTG) from S. cerevisiae (for examples from KAR2, CAGCGTG and PDI1, CACCGTG) [13, 14]. The similar UPRE-1 is also found in the promoter region of the P. pastoris KAR2 (CAGCGTG), INO1 (CAACTTG) and HAC1 (CAACTTG) genes [15]. The presence of an HAC1 UPRE implies that Hac1p can up-regulate its own transcription. Unconventional splicing of HAC1 mRNA after ER stress signaling generates the active form of basic leucine zipper (bZIP) transcription factor Hac1p, which binds to the UPRE [16]."[1]

Human genes

Interactions

Consensus sequences

The upstream activating sequence (UAS) for Hac1p is 5'-CAGCGTG-3'.[2]

Samplings

Copying 5'-CAGCGTG-3' in "⌘F" yields none between ZSCAN22 and A1BG and none between ZNF497 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence CAGCGTG (starting with SuccessablesHac.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

  1. negative strand, negative direction, looking for CAGCGTG, 0.
  2. negative strand, positive direction, looking for CAGCGTG, 0.
  3. positive strand, negative direction, looking for CAGCGTG, 1, CAGCGTG at 740.
  4. positive strand, positive direction, looking for CAGCGTG, 0.
  5. complement, negative strand, negative direction, looking for GTCGCAC, 1, GTCGCAC at 740.
  6. complement, negative strand, positive direction, looking for GTCGCAC, 0.
  7. complement, positive strand, negative direction, looking for GTCGCAC, 0.
  8. complement, positive strand, positive direction, looking for GTCGCAC, 0.
  9. inverse complement, negative strand, negative direction, looking for CACGCTG, 0.
  10. inverse complement, negative strand, positive direction, looking for CACGCTG, 1, CACGCTG at 778.
  11. inverse complement, positive strand, negative direction, looking for CACGCTG, 0.
  12. inverse complement, positive strand, positive direction, looking for CACGCTG, 0.
  13. inverse negative strand, negative direction, looking for GTGCGAC, 0.
  14. inverse negative strand, positive direction, looking for GTGCGAC, 0.
  15. inverse positive strand, negative direction, looking for GTGCGAC, 0.
  16. inverse positive strand, positive direction, looking for GTGCGAC, 1, GTGCGAC at 778.

Hac distal promoters

Positive strand: CAGCGTG at 740, and complement.

Negative strand: CACGCTG at 778, and complement.

See also

References

  1. Niwed Kullawong, Sutipa Tanapongpipat, Lily Eurwilaichitr and Witoon Tirasophon (2018). "Unfolded Protein Response (UPR) Induced Hybrid Promoters for Heterologous Gene Expression in Pichia pastoris" (PDF). Chiang Mai Journal of Science. 45 (7): 2554–2565. Retrieved 11 January 2021.
  2. Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check |pmid= value (help). Retrieved 18 September 2020.

External links