Aft1p gene transcriptions: Difference between revisions
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For the Basic programs testing consensus sequence 5'-(C/T)(A/G)CACCC(A/G)-3' (starting with SuccessablesAFT.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found: | For the Basic programs testing consensus sequence 5'-(C/T)(A/G)CACCC(A/G)-3' (starting with SuccessablesAFT.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found: | ||
# negative strand, negative direction, looking for 5'-(C/T)(A/G)CACCC(A/G)-3', 0. | # negative strand, negative direction, looking for 5'-(C/T)(A/G)CACCC(A/G)-3', 0. | ||
# negative strand, positive direction, looking for 5'-(C/T)(A/G)CACCC(A/G)-3', | # negative strand, positive direction, looking for 5'-(C/T)(A/G)CACCC(A/G)-3', 1, 5'-TGCACCCG-3' at 3324. | ||
# positive strand, negative direction, looking for 5'-(C/T)(A/G)CACCC(A/G)-3', 0. | # positive strand, negative direction, looking for 5'-(C/T)(A/G)CACCC(A/G)-3', 0. | ||
# positive strand, positive direction, looking for 5'-(C/T)(A/G)CACCC(A/G)-3', 0. | # positive strand, positive direction, looking for 5'-(C/T)(A/G)CACCC(A/G)-3', 0. | ||
| Line 36: | Line 36: | ||
# complement, negative strand, positive direction, looking for 5'-(A/G)(C/T)GTGGG(C/T)-3', 0. | # complement, negative strand, positive direction, looking for 5'-(A/G)(C/T)GTGGG(C/T)-3', 0. | ||
# complement, positive strand, negative direction, looking for 5'-(A/G)(C/T)GTGGG(C/T)-3', 0. | # complement, positive strand, negative direction, looking for 5'-(A/G)(C/T)GTGGG(C/T)-3', 0. | ||
# complement, positive strand, positive direction, looking for 5'-(A/G)(C/T)GTGGG(C/T)-3', | # complement, positive strand, positive direction, looking for 5'-(A/G)(C/T)GTGGG(C/T)-3', 1, 5'-ACGTGGGC-3' at 3324. | ||
# inverse complement, negative strand, negative direction, looking for 5'-(C/T)GGGTG(C/T)(A/G)-3', 0. | # inverse complement, negative strand, negative direction, looking for 5'-(C/T)GGGTG(C/T)(A/G)-3', 0. | ||
# inverse complement, negative strand, positive direction, looking for 5'-(C/T)GGGTG(C/T)(A/G)-3', 0. | # inverse complement, negative strand, positive direction, looking for 5'-(C/T)GGGTG(C/T)(A/G)-3', 0. | ||
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===AFT distal promoters=== | ===AFT distal promoters=== | ||
{{main|Distal promoter gene transcriptions}} | {{main|Distal promoter gene transcriptions}} | ||
Negative strand, positive direction: 5'-TGCACCCG-3' at 3324 and complement. | |||
==Acknowledgements== | ==Acknowledgements== | ||
Revision as of 15:54, 31 October 2020
Associate Editor(s)-in-Chief: Henry A. Hoff
Consensus sequences
The upstream activating sequence (UAS) for Aft1p is 5'-PyPuCACCCPu-3' or 5'-(C/T)(A/G)CACCC(A/G).[1]
Hypotheses
- A1BG has no Aft1p in either promoter.
- A1BG is not transcribed by an Aft1p.
- Aft1p does not participate in the transcription of A1BG.
Samplings
Copying 5'-TGCACCC-3' in "⌘F" yields none between ZSCAN22 and A1BG and one 5'-TGCACCCG-3' between ZNF497 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence 5'-(C/T)(A/G)CACCC(A/G)-3' (starting with SuccessablesAFT.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for 5'-(C/T)(A/G)CACCC(A/G)-3', 0.
- negative strand, positive direction, looking for 5'-(C/T)(A/G)CACCC(A/G)-3', 1, 5'-TGCACCCG-3' at 3324.
- positive strand, negative direction, looking for 5'-(C/T)(A/G)CACCC(A/G)-3', 0.
- positive strand, positive direction, looking for 5'-(C/T)(A/G)CACCC(A/G)-3', 0.
- complement, negative strand, negative direction, looking for 5'-(A/G)(C/T)GTGGG(C/T)-3', 0.
- complement, negative strand, positive direction, looking for 5'-(A/G)(C/T)GTGGG(C/T)-3', 0.
- complement, positive strand, negative direction, looking for 5'-(A/G)(C/T)GTGGG(C/T)-3', 0.
- complement, positive strand, positive direction, looking for 5'-(A/G)(C/T)GTGGG(C/T)-3', 1, 5'-ACGTGGGC-3' at 3324.
- inverse complement, negative strand, negative direction, looking for 5'-(C/T)GGGTG(C/T)(A/G)-3', 0.
- inverse complement, negative strand, positive direction, looking for 5'-(C/T)GGGTG(C/T)(A/G)-3', 0.
- inverse complement, positive strand, negative direction, looking for 5'-(C/T)GGGTG(C/T)(A/G)-3', 0.
- inverse complement, positive strand, positive direction, looking for 5'-(C/T)GGGTG(C/T)(A/G)-3', 0.
- inverse negative strand, negative direction, looking for 5'-(A/G)CCCAC(A/G)(C/T)-3', 0.
- inverse negative strand, positive direction, looking for 5'-(A/G)CCCAC(A/G)(C/T)-3', 0.
- inverse positive strand, negative direction, looking for 5'-(A/G)CCCAC(A/G)(C/T)-3', 0.
- inverse positive strand, positive direction, looking for 5'-(A/G)CCCAC(A/G)(C/T)-3', 0.
AFT core promoters
AFT proximal promoters
AFT distal promoters
Negative strand, positive direction: 5'-TGCACCCG-3' at 3324 and complement.
Acknowledgements
The content on this page was first contributed by: Henry A. Hoff.
See also
References
- ↑ Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check
|pmid=value (help). Retrieved 18 September 2020.