Proline

Proline
Systematic (IUPAC) name
(S)-Pyrrolidine-2- carboxylic acid
Identifiers
CAS number	147-85-3
PubChem	614
Chemical data
Formula	C5H9NO2
Molar mass	115.13
SMILES	OC(=O)[C@@H]1CCCN1
Complete data

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Proline (abbreviated as Pro or P) is an α-amino acid, one of the twenty DNA-encoded amino acids. Its codons are CCU, CCC, CCA, and CCG. It is not an essential amino acid, which means that humans can synthesize it. It is the unique proteogenic amino acid where the α-amino group is secondary.

Biosynthesis

Proline is biosynthetically derived from the amino acid L-glutamate and its immediate precursor is the imino acid (S)-Δ¹-pyrroline-5-carboxylate (P5C). Enzymes involved in a typical biosynthesis include:

1. glutamate kinase (ATP-dependent)
2. glutamate dehydrogenase (requires NADH or NADPH)
3. pyrroline-5-carboxylate reductase (requires NADH or NADPH)

Structural properties

The distinctive cyclic structure of proline's side chain locks its <math>\phi</math> backbone dihedral angle at approximately -75°, giving proline an exceptional conformational rigidity compared to other amino acids. Hence, proline loses less conformational entropy upon folding, which may account for its higher prevalence in the proteins of thermophilic organisms. Proline acts as a structural disruptor in the middle of regular secondary structure elements such as alpha helices and beta sheets; however, proline is commonly found as the first residue of an alpha helix and also in the edge strands of beta sheets. Proline is also commonly found in turns, which may account for the curious fact that proline is usually solvent-exposed, despite having a completely aliphatic side chain. Because proline lacks a hydrogen on the amide group, it cannot act as a hydrogen bond donor, only as a hydrogen bond acceptor.

Multiple prolines and/or hydroxyprolines in a row can create a polyproline helix, the predominant secondary structure in collagen. The hydroxylation of proline by prolyl hydroxylase (or other additions of electron-withdrawing substituents such as fluorine) increases the conformational stability of collagen significantly. Hence, the hydroxylation of proline is a critical biochemical process for maintaining the connective tissue of higher organisms. Severe diseases such as scurvy can result from defects in this hydroxylation, e.g., mutations in the enzyme prolyl hydroxylase or lack of the necessary ascorbate (vitamin C) cofactor.

Sequences of proline and 2-aminoisobutyric acid (Aib) also form a helical turn structure.

In 2006, scientists at ASU discovered that solutions of TiO2 illuminated with ultraviolet radiation can serve as an extremely cost-effective and accurate protein cleavage catalyst. The TiO2 catalyst preferentially and rapidly cleaves protein at sites where proline is present, while taking much longer to degrade the protein from its endpoints.

Cis-trans isomerization

Peptide bonds to proline and other N-substituted amino acids (such as sarcosine) are able to populate both the cis and trans isomers. Most peptide bonds prefer overwhelmingly to adopt the trans isomer (typically 99.9% under unstrained conditions), chiefly because the amide hydrogen (trans isomer) offers less steric repulsion to the preceding <math>\mathrm{C}^{\alpha}</math> atom than does the following <math>\mathrm{C}^{\alpha}</math> atom (cis isomer). By contrast, the cis and trans isomers of the X-Pro peptide bond both experience steric clashes with the neighboring subtitution and are nearly equally energetically disfavorable. Hence, the fraction of X-Pro peptide bonds in the cis isomer under unstrained conditions ranges from 10-40%; the fraction depends slightly on the preceding amino acid X, with aromatic residues favoring the cis isomer slightly.

From a kinetic standpoint, Cis-trans proline isomerization is a very slow process that can impede the progress of protein folding by trapping one or more prolines crucial for folding in the nonnative isomer, especially when the native protein requires the cis isomer. This is because proline residues are exclusively synthesized in the ribosome as the trans isomer form. All organisms possess prolyl isomerase enzymes to catalyze this isomerization, and some bacteria have specialized prolyl isomerases associated with the ribosome. However, not all prolines are essential for folding, and protein folding may proceed at a normal rate despite having non-native conformers of many X-Pro peptide bonds.

Usage

Proline and its derivatives are often used as asymmetric catalysts in organic reactions. The CBS reduction and proline catalysed aldol condensation are prominent examples.

L-proline is an ingredient in energy drinks such as "Sobe power fruit punch". Proline has a sweet flavor with a distinct aftertaste.

See also

• Collagen
• Polyproline helix
• Peptide bond (for more discussion of cis-trans isomerization)
• Hyperprolinemia

External links

• Proline biosynthesis
• Computational Chemistry Wiki
• Proline biosynthesis

References

• Balbach J, Schmid FX. (2000). Proline isomerization and its catalysis in protein folding. In Mechanisms of Protein Folding 2nd ed. Editor RH Pain. Oxford University Press.
• For a thorough scientific overview of disorders of proline and hydroxyproline metabolism, one can consult chapter 81 of OMMBID Charles Scriver, Beaudet, A.L., Valle, D., Sly, W.S., Vogelstein, B., Childs, B., Kinzler, K.W. (Accessed 2007). The Online Metabolic and Molecular Bases of Inherited Disease. New York: McGraw-Hill. - Summaries of 255 chapters, full text through many universities. There is also the OMMBID blog.
• For more online resources and references, see inborn errors of metabolism.

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| group5 = Clinical Trials Involving Proline | list5 = Ongoing Trials on Proline at Clinical Trials.gov • Trial results on Proline • Clinical Trials on Proline at Google

| group6 = Guidelines / Policies / Government Resources (FDA/CDC) Regarding Proline | list6 = US National Guidelines Clearinghouse on Proline • NICE Guidance on Proline • NHS PRODIGY Guidance • FDA on Proline • CDC on Proline

| group7 = Textbook Information on Proline | list7 = Books and Textbook Information on Proline

| group8 = Pharmacology Resources on Proline | list8 = AND (Dose)}} Dosing of Proline • AND (drug interactions)}} Drug interactions with Proline • AND (side effects)}} Side effects of Proline • AND (Allergy)}} Allergic reactions to Proline • AND (overdose)}} Overdose information on Proline • AND (carcinogenicity)}} Carcinogenicity information on Proline • AND (pregnancy)}} Proline in pregnancy • AND (pharmacokinetics)}} Pharmacokinetics of Proline •

| group9 = Genetics, Pharmacogenomics, and Proteinomics of Proline | list9 = AND (pharmacogenomics)}} Genetics of Proline • AND (pharmacogenomics)}} Pharmacogenomics of Proline • AND (proteomics)}} Proteomics of Proline

| group10 = Newstories on Proline | list10 = Proline in the news • Be alerted to news on Proline • News trends on Proline</small>

| group11 = Commentary on Proline | list11 = Blogs on Proline

| group12 = Patient Resources on Proline | list12 = Patient resources on Proline • Discussion groups on Proline • Patient Handouts on Proline • Directions to Hospitals Treating Proline • Risk calculators and risk factors for Proline

| group13 = Healthcare Provider Resources on Proline | list13 = Symptoms of Proline • Causes & Risk Factors for Proline • Diagnostic studies for Proline • Treatment of Proline

| group14 = Continuing Medical Education (CME) Programs on Proline | list14 = CME Programs on Proline

| group15 = International Resources on Proline | list15 = Proline en Espanol • Proline en Francais

| group16 = Business Resources on Proline | list16 = Proline in the Marketplace • Patents on Proline

| group17 = Informatics Resources on Proline | list17 = List of terms related to Proline

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