Amoebic liver abscess other diagnostic studies: Difference between revisions

Jump to navigation Jump to search
VisualWikitext
Line 23: Line 23:
* Less reliable method than culture or antigen detection test<ref name="pmid14557296">{{cite journal| author=Tanyuksel M, Petri WA| title=Laboratory diagnosis of amebiasis. | journal=Clin Microbiol Rev | year= 2003 | volume= 16 | issue= 4 | pages= 713-29 | pmid=14557296 | doi= | pmc=207118 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=14557296  }} </ref><ref name="pmid619763">{{cite journal| author=Krogstad DJ, Spencer HC, Healy GR, Gleason NN, Sexton DJ, Herron CA| title=Amebiasis: epidemiologic studies in the United States, 1971-1974. | journal=Ann Intern Med | year= 1978 | volume= 88 | issue= 1 | pages= 89-97 | pmid=619763 | doi= | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=619763  }} </ref>
* Less reliable method than culture or antigen detection test<ref name="pmid14557296">{{cite journal| author=Tanyuksel M, Petri WA| title=Laboratory diagnosis of amebiasis. | journal=Clin Microbiol Rev | year= 2003 | volume= 16 | issue= 4 | pages= 713-29 | pmid=14557296 | doi= | pmc=207118 | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=14557296  }} </ref><ref name="pmid619763">{{cite journal| author=Krogstad DJ, Spencer HC, Healy GR, Gleason NN, Sexton DJ, Herron CA| title=Amebiasis: epidemiologic studies in the United States, 1971-1974. | journal=Ann Intern Med | year= 1978 | volume= 88 | issue= 1 | pages= 89-97 | pmid=619763 | doi= | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=619763  }} </ref>
* MtermiMtenMlyMinimum three stool samples are examined for ova and parasites within 10days of sample collection as  organisms  are excreted intermittently<ref name="pmid8863036">{{cite journal| author=Li E, Stanley SL| title=Protozoa. Amebiasis. | journal=Gastroenterol Clin North Am | year= 1996 | volume= 25 | issue= 3 | pages= 471-92 | pmid=8863036 | doi= | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=8863036  }} </ref>
* MtermiMtenMlyMinimum three stool samples are examined for ova and parasites within 10days of sample collection as  organisms  are excreted intermittently<ref name="pmid8863036">{{cite journal| author=Li E, Stanley SL| title=Protozoa. Amebiasis. | journal=Gastroenterol Clin North Am | year= 1996 | volume= 25 | issue= 3 | pages= 471-92 | pmid=8863036 | doi= | pmc= | url=https://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=8863036  }} </ref>
* Presence of RBCs in trophozoites is diagnostic of Entamoeba hystolytica
* Presence of RBCs in trophozoites is diagnostic of Entamoeba histolytica
*
|-
|-
|'''Wet (Saline) preparation'''
|'''Wet (Saline) preparation'''

Revision as of 01:07, 10 February 2017

Liver abscess Main Page

Amoebic liver abscess Microchapters

Home

Patient Information

Overview

Historical Perspective

Classification

Pathophysiology

Causes

Differentiating amoebic liver abscess from other Diseases

Epidemiology and Demographics

Risk Factors

Screening

Natural History, Complications and Prognosis

Diagnosis

History and Symptoms

Physical Examination

Diagnostic Approach

Laboratory Findings

Electrocardiogram

Chest X Ray

CT

MRI

Ultrasound

Other Imaging Studies

Other Diagnostic Studies

Treatment

Medical Therapy

Surgery

Primary Prevention

Secondary Prevention

Cost-Effectiveness of Therapy

Future or Investigational Therapies

Case Studies

Case #1

Amoebic liver abscess other diagnostic studies On the Web

Most recent articles

Most cited articles

Review articles

CME Programs

Powerpoint slides

Images

American Roentgen Ray Society Images of Amoebic liver abscess other diagnostic studies

All Images
X-rays
Echo & Ultrasound
CT Images
MRI

Ongoing Trials at Clinical Trials.gov

US National Guidelines Clearinghouse

NICE Guidance

FDA on Amoebic liver abscess other diagnostic studies

CDC on Amoebic liver abscess other diagnostic studies

Amoebic liver abscess other diagnostic studies in the news

Blogs on Amoebic liver abscess other diagnostic studies

Directions to Hospitals Treating Type chapter name here

Risk calculators and risk factors for Amoebic liver abscess other diagnostic studies

Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1];Associate Editor(s)-in-Chief: Yamuna Kondapally, M.B.B.S[2]

Overview

Other Diagnostic Tests

Needle aspiration

  • Needle aspiration is used to differentiate between amoebic and pyogenic liver abscess
  • The ultrasound and CT are used to guide percutaneous aspiration and drainage.
  • The aspirated fluid is odorless unless secondarily infected
  • The pathognomonic of the aspirate is the reddish-brown anchovy paste appearance, which indicates that the abscess has been present for weeks.
  • The trophozoites are present only in the wall of the abscess. Rest of abscess is composed of lysed leukocytes.
Laboratory Method Findings
Microscopy
  • Microscopic techniques include 1. Wet preparation 2. Concentration 3. Permanently stained smears[1]
  • Less reliable method than culture or antigen detection test[2][3]
  • MtermiMtenMlyMinimum three stool samples are examined for ova and parasites within 10days of sample collection as organisms are excreted intermittently[4]
  • Presence of RBCs in trophozoites is diagnostic of Entamoeba histolytica
Wet (Saline) preparation
  • Very insensitive method (<10%) Procedure
  • The sample is a fresh specimen that should be examined within 1 hr of collection
  • The test is positive when RBCs in trophozoites are detected
  • It is not used in patients without acute dysentery as trophozoites will not contain RBCs
 Concentration Technique
  • It is helpful in detecting cysts in the stool sample in asymptomatic carriers
 Permanently stained smears
  • This is an important method for identification and recovery of Entamoeba species
Culture Methods
Isoenzyme analysis
Antibody Detection Tests
Antigen Detection Tests
Immunochromatographic Assays

DNA-Based Diagnostic Tests

Laboratory Methods Findings
Manual Methods
Automated Methods
Conventional PCR
Real-Time PCR
Microarray Development
Typing Methods

References

  1. ↑ Huston CD, Haque R, Petri WA (1999). "Molecular-based diagnosis of Entamoeba histolytica infection". Expert Rev Mol Med. 1999: 1–11. doi:doi:10.1017/S1462399499000599 Check |doi= value (help). PMID 14987356.
  2. ↑ Tanyuksel M, Petri WA (2003). "Laboratory diagnosis of amebiasis". Clin Microbiol Rev. 16 (4): 713–29. PMC 207118. PMID 14557296.
  3. ↑ Krogstad DJ, Spencer HC, Healy GR, Gleason NN, Sexton DJ, Herron CA (1978). "Amebiasis: epidemiologic studies in the United States, 1971-1974". Ann Intern Med. 88 (1): 89–97. PMID 619763.
  4. ↑ Li E, Stanley SL (1996). "Protozoa. Amebiasis". Gastroenterol Clin North Am. 25 (3): 471–92. PMID 8863036.
Retrieved from "https://www.wikidoc.org/index.php?title=Amoebic_liver_abscess_other_diagnostic_studies&oldid=1292562"