Pertussis other diagnostic studies: Difference between revisions
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__NOTOC__ | __NOTOC__ | ||
{{CMG}}; {{AE}} {{ADI}} | {{CMG}}; {{AE}} {{ADI}} {{LRO}} | ||
{{Pertussis}} | {{Pertussis}} | ||
==Overview | |||
Pertussis can be diagnosed through the Polymerase Chain Reaction (PCR) test, as well as the Direct Flourescent Antibody (DFA) and Pulsed-field Gel Eletrophoresis tests. | |||
==Other Diagnostic Studies== | ==Other Diagnostic Studies== | ||
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** DFA's drawbacks include poor sensitivity and specificity.<ref name = PMC85400>{{cite journal |vauthors=Miagkaia IP |title=[Antireticular cytotoxic serum as an agent for stimulating the immunity system] |language=Russian |journal=Zh. Mikrobiol. Epidemiol. Immunobiol. |volume= |issue=12 |pages=82–6 |year=1978 |pmid=85400 |doi= |url=}}</ref> | ** DFA's drawbacks include poor sensitivity and specificity.<ref name = PMC85400>{{cite journal |vauthors=Miagkaia IP |title=[Antireticular cytotoxic serum as an agent for stimulating the immunity system] |language=Russian |journal=Zh. Mikrobiol. Epidemiol. Immunobiol. |volume= |issue=12 |pages=82–6 |year=1978 |pmid=85400 |doi= |url=}}</ref> | ||
* Pulsed-field gel electrophoresis (PFGE) | * Pulsed-field gel electrophoresis (PFGE) | ||
** PFGE is a[[DNA fingerprinting]] technique that has the following advantages:<ref name = "PMC446263">{{cite journal |vauthors=Brown DD, Juhl RP, Warner SL |title=Effects of hypocholesterolemic interventions on digoxin bioavailability |journal=Drug Metab. Rev. |volume=9 |issue=1 |pages=107–17 |year=1979 |pmid=446263 |doi= |url=}}</ref> | ** PFGE is a [[DNA fingerprinting]] technique that has the following advantages:<ref name = "PMC446263">{{cite journal |vauthors=Brown DD, Juhl RP, Warner SL |title=Effects of hypocholesterolemic interventions on digoxin bioavailability |journal=Drug Metab. Rev. |volume=9 |issue=1 |pages=107–17 |year=1979 |pmid=446263 |doi= |url=}}</ref> | ||
***Highly discriminating in its results.<ref name = "CDCTest">{{cite web |url=http://www.cdc.gov/pulsenet/pathogens/pfge.html |title=Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC |format= |work= |accessdate=}}</ref> | ***Highly discriminating in its results.<ref name = "CDCTest">{{cite web |url=http://www.cdc.gov/pulsenet/pathogens/pfge.html |title=Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC |format= |work= |accessdate=}}</ref> | ||
***Easily applicable to [[Bordetella pertussis]].<ref name = "CDCTest">{{cite web |url=http://www.cdc.gov/pulsenet/pathogens/pfge.html |title=Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC |format= |work= |accessdate=}}</ref> | ***Easily applicable to [[Bordetella pertussis]].<ref name = "CDCTest">{{cite web |url=http://www.cdc.gov/pulsenet/pathogens/pfge.html |title=Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC |format= |work= |accessdate=}}</ref> | ||
***Stable and easily reproduced.<ref name = "CDCTest">{{cite web |url=http://www.cdc.gov/pulsenet/pathogens/pfge.html |title=Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC |format= |work= |accessdate=}}</ref> | ***Stable and easily reproduced.<ref name = "CDCTest">{{cite web |url=http://www.cdc.gov/pulsenet/pathogens/pfge.html |title=Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC |format= |work= |accessdate=}}</ref> | ||
** PFGE's drawbacks include duration of time to perform as well as required a specifically trained and skilled technician.<ref name = "CDCTest">{{cite web |url=http://www.cdc.gov/pulsenet/pathogens/pfge.html |title=Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC |format= |work= |accessdate=}}</ref> | |||
==References== | ==References== |
Revision as of 15:23, 7 January 2016
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Aditya Govindavarjhulla, M.B.B.S. [2] Luke Rusowicz-Orazem, B.S.
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==Overview Pertussis can be diagnosed through the Polymerase Chain Reaction (PCR) test, as well as the Direct Flourescent Antibody (DFA) and Pulsed-field Gel Eletrophoresis tests.
Other Diagnostic Studies
- Polymerase chain reaction (PCR)
- Direct fluorescent antibody (DFA) testing
- Pulsed-field gel electrophoresis (PFGE)
- PFGE is a DNA fingerprinting technique that has the following advantages:[3]
- Highly discriminating in its results.[4]
- Easily applicable to Bordetella pertussis.[4]
- Stable and easily reproduced.[4]
- PFGE's drawbacks include duration of time to perform as well as required a specifically trained and skilled technician.[4]
- PFGE is a DNA fingerprinting technique that has the following advantages:[3]
References
- ↑ 1.0 1.1 "Pertussis | Whooping Cough | Diagnosis Confirmation | CDC".
- ↑ 2.0 2.1 Miagkaia IP (1978). "[Antireticular cytotoxic serum as an agent for stimulating the immunity system]". Zh. Mikrobiol. Epidemiol. Immunobiol. (in Russian) (12): 82–6. PMID 85400.
- ↑ Brown DD, Juhl RP, Warner SL (1979). "Effects of hypocholesterolemic interventions on digoxin bioavailability". Drug Metab. Rev. 9 (1): 107–17. PMID 446263.
- ↑ 4.0 4.1 4.2 4.3 "Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC".