Pertussis other diagnostic studies

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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Serge Korjian M.D.; Aditya Govindavarjhulla, M.B.B.S. [2]; Luke Rusowicz-Orazem, B.S.; Yazan Daaboul, M.D.


Pertussis can also be diagnosed using direct flourescent antibody (DFA) and pulsed-field gel eletrophoresis tests.

Other Diagnostic Studies

  • Direct fluorescent antibody (DFA)
    • DFA is inexpensive and may provide rapid results.[1]
    • It's drawbacks, however, include poor sensitivity and specificity.[1]
  • Pulsed-field gel electrophoresis (PFGE)
    • PFGE is a DNA fingerprinting technique that has the following advantages:[2]
      • Highly discriminating in its results[3]
      • Easily applicable to Bordetella pertussis[3]
      • Stable and easily reproduced[3]
    • PFGE's drawbacks include duration of time to perform as well as required a specifically trained and skilled technician.[3]


  1. 1.0 1.1 Miagkaia IP (1978). "[Antireticular cytotoxic serum as an agent for stimulating the immunity system]". Zh. Mikrobiol. Epidemiol. Immunobiol. (in Russian) (12): 82–6. PMID 85400.
  2. Brown DD, Juhl RP, Warner SL (1979). "Effects of hypocholesterolemic interventions on digoxin bioavailability". Drug Metab. Rev. 9 (1): 107–17. PMID 446263.
  3. 3.0 3.1 3.2 3.3 "Pulsed-field Gel Electrophoresis (PFGE) | Pathogens and Protocols | PulseNet | CDC".

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