Protein disulfide isomerase
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| protein disulfide isomerase family A, member 2
| |
| Identifiers | |
| Symbol | PDIA2 |
| Alt. Symbols | PDIP |
| Entrez | 64714 |
| HUGO | 14180 |
| OMIM | 608012 |
| RefSeq | NM_006849 |
| UniProt | Q13087 |
| Other data | |
| Locus | Chr. 16 p13.3 |
| Identifiers | |
| Symbol | PDIA3 |
| Alt. Symbols | GRP58 |
| Entrez | 2923 |
| HUGO | 4606 |
| OMIM | 602046 |
| RefSeq | NM_005313 |
| UniProt | P30101 |
| Other data | |
| Locus | Chr. 15 q15 |
| protein disulfide isomerase family A, member 4
| |
| Identifiers | |
| Symbol | PDIA4 |
| Entrez | 9601 |
| HUGO | 30167 |
| RefSeq | NM_004911 |
| UniProt | P13667 |
| Other data | |
| Locus | Chr. 7 q35 |
| protein disulfide isomerase family A, member 5
| |
| Identifiers | |
| Symbol | PDIA5 |
| Entrez | 10954 |
| HUGO | 24811 |
| RefSeq | NM_006810 |
| UniProt | Q14554 |
| Other data | |
| EC number | 5.3.4.1 |
| Locus | Chr. 3 q21.1 |
| protein disulfide isomerase family A, member 6
| |
| Identifiers | |
| Symbol | PDIA6 |
| Alt. Symbols | TXNDC7 |
| Entrez | 10130 |
| HUGO | 30168 |
| RefSeq | NM_005742 |
| UniProt | Q15084 |
| Other data | |
| Locus | Chr. 2 p25.1 |
Protein disulfide isomerase or PDI (EC 5.3.4.1) is an enzyme in the endoplasmic reticulum in eukaryotes or periplasmic space of prokaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. This allows proteins to quickly find the correct arrangement of disulfide bonds in their fully-folded state, and therefore the enzyme acts to catalyze protein folding. In contrast, reduced (dithiol) form of PDI is able to catalyse a reduction of mispaired thiol residues of a particular substrate, acting as an isomerase. Therefore, PDI is capable of catalyzing the post-translational disulfide exchange. Such exchange reactions can occur intramolecularly, leading to the rearrangement of disulphide bonds in a single protein.
Another major function of PDI relates to its activity as a chaperone, i.e., it aids wrongly-folded proteins to reach a correctly-folded state without the aid of enzymatic disulfide shuffling.
PDI has been found to be involved in the breaking of bonds on the HIV gp120 protein during HIV infection of CD4 positive cells, and is required for HIV infection of lymphocytes and monoctyes. Some studies have shown it to be available for HIV infection on the surface of the cell clustered around the CD4 protein. Yet conflicting studies have shown that it is not available on the cell surface, but instead is found in significant amounts in the blood plasma.
Oxidized PDI can catalyze the formation of a disulfide bridge. This reduces PDI and a protein called Ero1 oxidizes it again.
Other functions
PDI helps load antigenic peptides into MHC class I molecules. These molecules (MHC I) are related to the peptide presentation by APC in the immunity response.
Assays used for PDI activity
Insulin Turbidity Assay: PDI breaks the two disulfide bonds between two insulin (a and b) chains that results in precipitation of b chain. This precipitation can be monitored at 620 nm, which is indirectly used monitor PDI activity[1]. Sensitivity of this assay is in micromolar range.
ScRNase assay: PDI converts scrambled (inactive) RNase into native (active) RNase that further acts on its substrate[1]. The sensitivty is in micromolar range.
Di-E-GSSG assay: This is the fluorometric assay that can detect picomolar quantities of PDI and therefore is the most sensitive assay to date for detecting PDI activity[1]. Di-E-GSSG has two eosin molecules attached to oxidized glutathione (GSSG). The proximity of eosin molecules leads to the quenching of its fluorescence. However, upon breakage of disulfide bond by PDI, fluorescence increases 70-fold.
References
Intramolecular oxidoreductases isomerases (EC 5.3) | |
|---|---|
| 5.3.1 - Aldoses/Ketoses | Triosephosphate isomerase - Phosphopentose isomerase - Mannose phosphate isomerase - Glucose isomerase |
| 5.3.3 - C=C | Isopentenyl-diphosphate delta isomerase - Enoyl CoA isomerase |
| 5.3.4 - S-S | Protein disulfide isomerase (PDIA3) |
| 5.3.99 - other | Prostaglandin D2 synthase - Prostaglandin E synthase - Prostacyclin synthase - Thromboxane-A synthase |
fr:Protein disulfide isomerase
Acknowledgement and Attribution Regarding Sources of Content
Some of the initial content on this page may be incorporated in part from copyleft sources in the public domain including wikis such as Wikipedia and AskDrWiki. Drug information for patients came from the The National Library of Medicine. Infectious disease information may have come from the Centers for Disease Control (CDC). Differential Diagnoses are drawn from clinicians as well as an amalgamation of 3 sources: 1.The Disease Database; 2. Kahan, Scott, Smith, Ellen G. In A Page: Signs and Symptoms. Malden, Massachusetts: Blackwell Publishing, 2004:3; 3. Sailer, Christian, Wasner, Susanne. Differential Diagnosis Pocket. Hermosa Beach, CA: Borm Bruckmeir Publishing LLC, 2002:7 .
