Leptospira

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For the brachiopod genus, see Leptospira (brachiopod).
Leptospira
Scanning electron micrograph of Leptospira interrogans.
Scanning electron micrograph of Leptospira interrogans.
Scientific classification
Kingdom: Monera
Phylum: Spirochaetes
Class: Spirochaetes
Order: Spirochaetales
Family: Leptospiraceae
Genus: Leptospira
Noguchi 1917
Species

L. alexanderi
L. biflexa
L. broomii
L. borgpetersenii
L. fainei
L. inadai
L. interrogans
L. kirschneri
L. meyeri
L. noguchii
L. santarosai
L. weilii

L. genomospecies 1
L. genomospecies 3
L. genomospecies 4
L. genomospecies 5

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This page is about microbiologic aspects of the organism(s).  For clinical aspects of the disease, see Leptospirosis.

Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]

Overview

Leptospira (from the Greek leptos, meaning fine or thin, and the Latin spira, meaning coil)[1] is a genus of spirochaete bacteria, including a small number of pathogenic and saprophytic species.[2] Leptospira was first observed in 1907 in kidney tissue slices of a leptospirosis victim who was described as having died of "yellow fever."[3]

Taxonomy

Leptospira, together with the genera Leptonema and Turneria, is a member of the family Leptospiraceae. The genus Leptospira is divided into 17 genomospecies based on DNA hybridization studies.[4][5][6] However, at least one additional species which is yet to be named has been identified.[7]

Known pathogenic Leptospira

Leptospira interrogans
Leptospira kirschneri
Leptospira noguchii
Leptospira alexanderi
Leptospira weilii
Leptospira genomospecies 1,
Leptospira borgpetersenii
Leptospira santarosai

Intermediates or opportunistic Leptospira

Leptospira inadai
Leptospira fainei
Leptospira broomii

Non-pathogenic Leptospira

Leptospira biflexa
Leptospira meyeri
Leptospira wolbachii
Leptospira genomospecies 3
Leptospira genomospecies 4
Leptospira genomospecies 5

Members of Leptospira are also grouped into serovars according to their antigenic relatedness. There are currently over 200 recognized serovars. A few serovars are found in more than one species of Leptospira.

At its 2002 meeting, the Committee on the Taxonomy of Leptospira of the International Union of Microbiological Societies approved the following nomenclature for serovars of Leptospira. Genus and species must of course be italicized, with the serovar name not italicized and with an upper case first letter.

Genus species serovar Serovar_name

For example:

  • Leptospira interrogans serovar Australis
  • Leptospira biflexa serovar Patoc

Morphology

Although over 200 serovars of Leptospira have been described, all members of the genus have similar morphology. Leptospira are spiral-shaped bacteria that are 6-20 μm long and 0.1 μm in diameter with a wavelength of about 0.5 μm.[8] One or both ends of the spirochete are usually hooked. Because they are so thin, live Leptospira are best observed by darkfield microscopy.

The bacteria have a number of freedom degrees; when ready to proliferate via binary fission, the bacterium noticeably bends in the place of the future split.

Cellular structure

Leptospira have a Gram-negative-like cell envelope consisting of a cytoplasmic and outer membrane. However, the peptidoglycan layer is associated with the cytoplasmic rather than the outer membrane, an arrangement that is unique to spirochetes. The two flagella of Leptospira extend from the cytoplasmic membrane at the ends of the bacteria into the periplasmic space and are necessary for the motility of Leptospira.[9]

The outer membrane contains a variety of lipoproteins and transmembrane outer membrane proteins.[10] As expected, the protein composition of the outer membrane differs when comparing Leptospira growing in artificial medium with Leptospira present in an infected animal.[11][12][13] Several leptospiral outer membrane proteins have been shown to attach to the host extracellular matrix and to factor H. These proteins may be important for adhesion of Leptospira to host tissues and in resisting complement, respectively.[14][15][16]

The outer membrane of Leptospira, like those of most other Gram-negative bacteria, contains lipopolysaccharide (LPS). Differences in the highly immunogenic LPS structure account for the numerous serovars of Leptospira.[8]. Consequently, immunity is serovar specific; current leptospiral vaccines, which consist of one or several serovars of Leptospira endemic in the population to be immunized, protect only against the serovars contained in the vaccine preparation. Leptospiral LPS has low endotoxin activity.[8]. An unusual feature of leptospiral LPS is that it activates host cells via TLR2 rather than TLR4.[17] The unique structure of the lipid A portion of the LPS molecule may acccount for this observation.[18] Finally, the LPS O antigen content of L. interrogans differs in an acutely infected versus a chronically infected animal.[19] The role of O antigen changes in the establishment or maintenance of acute or chronic infection, if any, is unknown.

Habitat

Leptospira, both pathogenic and saprotrophic, can occupy diverse environments, habitats and life cycles; it generally recognized these bacteria are virtually ubiquitous in terms of geographic distribution (present everywhere except Antarctica).[20]

Most of Leptospira, however, are hydrophilic - high humidity and neutral (6.9-7.4) pH are essential for their survival in the environment, with stagnant water reservoirs - bogs, shallow lakes, ponds, puddles, etc - being natural placeholder for the bacteria.

Nutrition

Leptospira are typically cultivated at 30 °C in Ellinghausen-McCullough-Johnson-Harris (EMJH) medium, which can be supplemented with 0.2-1% rabbit serum to enhance growth of fastidious strains.[21] Growth of pathogenic Leptospira in an artificial nutrient environment such as EMJH becomes noticeable in 4-7 days; growth of saprophytic strains occur within 2-3 days. The minimal growth temperature of pathogenic species is 13-15 °C. Because the minimal growth temperature of the saprophytes is 5-10 °C, the ability of Leptospira to grow at 13 °C can be used to distinguish saprophytic from pathogenic Leptospira species.[21] The optimal pH for growth of Leptospira is 7.2-7.6.

Leptospira are aerobes whose major carbon and energy source during in vitro growth is long-chain fatty acids, which are metabolized by beta-oxidation.[22][23] Fatty acids are provided in EMJH in the form of Tween.[21] Fatty acid molecules are bound by albumin in EMJH and are released slowly into the medium to prevent its toxic accumulation.

Like most bacteria, Leptospira require iron for growth.[24] L. interrogans and L. biflexa have the ability to acquire iron in different forms.[25] A TonB-dependent receptor required for utilization of the ferrous form of the iron has been identified in L. biflexa, and an ortholog of the receptor is encoded in the genome of L. interrogans. L. interrogans can also obtain iron from heme, which is bound to most of the iron in the human body. The HbpA hemin-binding protein, which may be involved in the uptake of hemin, has been identified on the surface of L. interrogans[26] Although other pathogenic species of Leptospira and L. biflexa lack HbpA, yet another hemin-binding protein, LipL41, may account for their ability to use hemin as a source of iron.[26] Although they do not secrete siderophores, L. biflexa and L. interrogans may be capable of obtaining iron from siderophores secreted by other microorganisms.[25]

Genome

The genome of pathogenic Leptospira consists of two chromosomes. The size of the genomes of L. interrogans serovars Copenhageni and Lai is approximately 4.6 Mb.[27][28] However, the genome of L. borgpetersenii serovar Hardjo is only 3.9 Mb in size with a large number of pseudogenes, gene fragments, and insertion sequences relative to the genomes of L. interrogans.[29] L. borgpetersenii serovar Hardjo is usually transmitted by direct exposure to infected tissues, whereas L. interrogans is often acquired from water or soil contaminated by the urine of carrier animals harboring Leptospira in their kidneys. The high number of defective genes and insertion sequences in L. borgpetersenii Hardjo together with the poor survival outside of the host and difference in transmission patterns compared to L. interrogans suggest that L. borgpetersenii is undergoing insertion-sequence mediated genomic decay, with ongoing loss of genes necessary for survival outside of the host animal.[29]

See also

References

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  2. Ryan KJ; Ray CG (editors) (2004). Sherris Medical Microbiology (4th ed. ed.). McGraw Hill. ISBN 0-8385-8529-9.
  3. Stimson AM (1907). "Note on an organism found in yellow-fever tissue." Public Health Reports 22:541.
  4. Brenner DJ, Kaufmann AF, Sulzer KR, Steigerwalt AG, Rogers FC, Weyant RS (1999). "Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies". Int. J. Syst. Bacteriol. 49 Pt 2: 839–58. PMID 10319510.
  5. Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, Levett PN, Gilman RH, Willig MR, Gotuzzo E, Vinetz JM (2003). "Leptospirosis: a zoonotic disease of global importance". The Lancet infectious diseases. 3 (12): 757–71. PMID 14652202.
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  9. Picardeau M, Brenot A, Saint Girons I (2001). "First evidence for gene replacement in Leptospira spp. Inactivation of L. biflexa flaB results in non-motile mutants deficient in endoflagella". Mol. Microbiol. 40 (1): 189–99. PMID 11298286.
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  11. Haake DA, Martinich C, Summers TA, Shang ES, Pruetz JD, McCoy AM, Mazel MK, Bolin CA (1998). "Characterization of leptospiral outer membrane lipoprotein LipL36: downregulation associated with late-log-phase growth and mammalian infection". Infect. Immun. 66 (4): 1579–87. PMID 9529084.
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  16. Choy HA, Kelley MM, Chen TL, Møller AK, Matsunaga J, Haake DA (2007). "Physiological osmotic induction of Leptospira interrogans adhesion: LigA and LigB bind extracellular matrix proteins and fibrinogen". Infect. Immun. 75 (5): 2441–50. doi:10.1128/IAI.01635-06. PMID 17296754.
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  20. Madigan M; Martinko J (editors). (2005). Brock Biology of Microorganisms (11th ed. ed.). Prentice Hall. ISBN 0-13-144329-1.
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  27. Ren SX, Fu G, Jiang XG, Zeng R, Miao YG, Xu H, Zhang YX, Xiong H, Lu G, Lu LF, Jiang HQ, Jia J, Tu YF, Jiang JX, Gu WY, Zhang YQ, Cai Z, Sheng HH, Yin HF, Zhang Y, Zhu GF, Wan M, Huang HL, Qian Z, Wang SY, Ma W, Yao ZJ, Shen Y, Qiang BQ, Xia QC, Guo XK, Danchin A, Saint Girons I, Somerville RL, Wen YM, Shi MH, Chen Z, Xu JG, Zhao GP (2003). "Unique physiological and pathogenic features of Leptospira interrogans revealed by whole-genome sequencing". Nature. 422 (6934): 888–93. doi:10.1038/nature01597. PMID 12712204.
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