Bacterial conjugation

Jump to navigation Jump to search

WikiDoc Resources for Bacterial conjugation

Articles

Most recent articles on Bacterial conjugation

Most cited articles on Bacterial conjugation

Review articles on Bacterial conjugation

Articles on Bacterial conjugation in N Eng J Med, Lancet, BMJ

Media

Powerpoint slides on Bacterial conjugation

Images of Bacterial conjugation

Photos of Bacterial conjugation

Podcasts & MP3s on Bacterial conjugation

Videos on Bacterial conjugation

Evidence Based Medicine

Cochrane Collaboration on Bacterial conjugation

Bandolier on Bacterial conjugation

TRIP on Bacterial conjugation

Clinical Trials

Ongoing Trials on Bacterial conjugation at Clinical Trials.gov

Trial results on Bacterial conjugation

Clinical Trials on Bacterial conjugation at Google

Guidelines / Policies / Govt

US National Guidelines Clearinghouse on Bacterial conjugation

NICE Guidance on Bacterial conjugation

NHS PRODIGY Guidance

FDA on Bacterial conjugation

CDC on Bacterial conjugation

Books

Books on Bacterial conjugation

News

Bacterial conjugation in the news

Be alerted to news on Bacterial conjugation

News trends on Bacterial conjugation

Commentary

Blogs on Bacterial conjugation

Definitions

Definitions of Bacterial conjugation

Patient Resources / Community

Patient resources on Bacterial conjugation

Discussion groups on Bacterial conjugation

Patient Handouts on Bacterial conjugation

Directions to Hospitals Treating Bacterial conjugation

Risk calculators and risk factors for Bacterial conjugation

Healthcare Provider Resources

Symptoms of Bacterial conjugation

Causes & Risk Factors for Bacterial conjugation

Diagnostic studies for Bacterial conjugation

Treatment of Bacterial conjugation

Continuing Medical Education (CME)

CME Programs on Bacterial conjugation

International

Bacterial conjugation en Espanol

Bacterial conjugation en Francais

Business

Bacterial conjugation in the Marketplace

Patents on Bacterial conjugation

Experimental / Informatics

List of terms related to Bacterial conjugation


Overview

Bacterial conjugation is the transfer of genetic material between bacteria through direct cell-to-cell contact.[1] Discovered in 1946 by Joshua Lederberg and Edward Tatum,[2] conjugation is a mechanism of horizontal gene transfer—as are transformation and transduction—although these mechanisms do not involve cell-to-cell contact.[3]

Bacterial conjugation is often incorrectly regarded as the bacterial equivalent of sexual reproduction or mating. It is not actually sexual, as it does not involve the fusing of gametes and the creation of a zygote, nor is there equal exchange of genetic material. It is merely the transfer of genetic information from a donor cell to a recipient. In order to perform conjugation, one of the bacteria, the donor, must play host to a conjugative or mobilizable genetic element, most often a conjugative or mobilizable plasmid or transposon.[4] Most conjugative plasmids have systems ensuring that the recipient cell does not already contain a similar element.

The genetic information transferred is often beneficial to the recipient cell. Benefits may include antibiotic resistance, other xenobiotic tolerance, or the ability to utilize a new metabolite.[5] Such beneficial plasmids may be considered bacterial endosymbionts. Some conjugative elements may also be viewed as genetic parasites on the bacterium, and conjugation as a mechanism was evolved by the mobile element to spread itself into new hosts.

Mechanism

The prototype for conjugative plasmids is the F-plasmid, also called the F-factor.[1] The F-plasmid is an episome (a plasmid that can integrate itself into the bacterial chromosome by genetic recombination) of about 100 kb length. (One kb is one thousand base pairs) It carries its own origin of replication, called oriT.[4] There can only be one copy of the F-plasmid in a given bacterium, either free or integrated (two immediately before cell division). The host bacterium is called F-positive or F-plus (denoted F+). Strains that lack F plasmids are called F-negative or F-minus (F-).

Among other genetic information, the F-plasmid carries a tra and a trb locus, which together are about 33 kb long and consist of about 40 genes. The tra locus includes the pilin gene and regulatory genes, which together form pili on the cell surface, polymeric proteins that can attach themselves to the surface of F- bacteria and initiate the conjugation. Though there is some debate on the exact mechanism, the pili themselves do not seem to be the structures through which the actual exchange of DNA takes place; rather, some proteins coded in the tra or trb loci seem to open a channel between the bacteria.

When conjugation is initiated, via a mating signal, a relaxase enzyme creates a nick in one plasmid DNA strand at the origin of transfer, or oriT. The relaxase may work alone or in a complex of over a dozen proteins, known collectively as a relaxosome. In the F-plasmid system, the relaxase enzyme is called TraI and the relaxosome consists of TraI, TraY, TraM, and the integrated host factor, IHF. The transferred, or T-strand, is unwound from the duplex plasmid and transferred into the recipient bacterium in a 5'-terminus to 3'-terminus direction. The remaining strand is replicated, either independent of conjugative action (vegetative replication, beginning at the oriV) or in concert with conjugation (conjugative replication similar to the rolling circle replication of lambda phage). Conjugative replication may necessitate a second nick before successful transfer can occur. A recent report claims to have inhibited conjugation with chemicals that mimic an intermediate step of this second nicking event.[6]

If the F-plasmid becomes integrated into the host genome, donor chromosomal DNA may be transferred along with plasmid DNA.[3] The certain amount of chromosomal DNA that is transferred depends on how long the bacteria remain in contact; for common laboratory strains of E. coli the transfer of the entire bacterial chromosome takes about 100 minutes. The transferred DNA can be integrated into the recipient genome via recombination.

A culture of cells containing non-integrated F plasmids usually contains a few that have accidentally become integrated, and these are responsible for those low-frequency chromosomal gene transfers which do occur in such cultures. Some strains of bacteria with an integrated F-plasmid can be isolated and grown in pure culture. Because such strains transfer chromosomal genes very efficiently, they are called Hfr (high frequency of recombination). The E. coli genome was originally mapped by interrupted mating experiments, in which various Hfr cells in the process of conjugation were sheared from recipients after less than 100 minutes (initially using a Waring blender) and investigating which genes were transferred.

Inter-Kingdom transfer

The nitrogen fixing Rhizobia are an interesting case, wherein conjugative elements naturally engage in inter-kingdom conjugation. Such elements as the Agrobacterium Ti or Ri plasmids contain elements can transfer to plant cells. Transferred genes enter the plant cell nucleus and effectively transform the plant cells into factories for the production of opines, which the bacteria use as carbon and energy sources. Infected plant cells form crown gall or root tumors. The Ti and Ri plasmids are thus endosymbionts of the bacteria, which are in turn endosymbionts (or parasites) of the infected plant.

The Ti and Ri plasmids are themselves conjugative. Ti and Ri transfer between bacteria uses an independent system (the tra, or transfer, operon) from that for inter-kingdom transfer (the vir, or virulence, operon). Such transfer creates virulent strains from previously avirulent Agrobacteria.

See also

References

  1. 1.0 1.1 Holmes RK, Jobling MG (1996). Genetics: Conjugation. in: Baron's Medical Microbiology (Baron S et al, eds.) (4th ed. ed.). Univ of Texas Medical Branch. ISBN 0-9631172-1-1.
  2. Lederberg J, Tatum EL (1946). "Gene recombination in E. coli". Nature. 158: 558.
  3. 3.0 3.1 Griffiths AJF; et al. (1999). An Introduction to genetic analysis (7th ed. ed.). San Francisco: W.H. Freeman. ISBN 0-7167-3520-2.
  4. 4.0 4.1 Ryan KJ, Ray CG (editors) (2004). Sherris Medical Microbiology (4th ed. ed.). McGraw Hill. pp. p. 60–4. ISBN 0838585299.
  5. Holmes RK, Jobling MG (1996). Genetics: Exchange of Genetic Information. in: Baron's Medical Microbiology (Baron S et al, eds.) (4th ed. ed.). Univ of Texas Medical Branch. ISBN 0-9631172-1-1.
  6. Lujan SA, Guogas LM, Ragonese H, Matson SW, Redinbo MR (2007). "Disrupting antibiotic resistance propagation by inhibiting the conjugative DNA relaxase". PNAS. 104: 12282–7.

External links

Template:Genetic recombination


Template:WikiDoc Sources