alpha-Mannosidase

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File:Tonge redo alpha mannosidase.png
Cartoon depiction of the protein Streptococcus pyogenes family GH38 α-Mannosidase created using PyMol.[1][2]

alpha-mannosidase
270px
alpha-mannosidase 1, tetramer, Saccharomyces cerevisiae
Identifiers
EC number3.2.1.24
CAS number9025-42-7
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

alpha-Mannosidase (EC 3.2.1.24, alpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, 1,2-alpha-mannosidase, 1,2-alpha-D-mannosidase, exo-alpha-mannosidase) is an enzyme involved in the cleavage of the alpha form of mannose. Its systematic name is alpha-D-mannoside mannohydrolase.[3][4]

Isozymes

Humans express the following three alpha-mannosidase isozymes:

mannosidase, alpha, class 2B, member 1
Identifiers
SymbolMAN2B1
Alt. symbolsMANB
Entrez4125
HUGO6826
OMIM609458
RefSeqNM_000528
UniProtO00754
Other data
EC number3.2.1.24
LocusChr. 19 cen-q13.1
mannosidase, alpha, class 2B, member 2
Identifiers
SymbolMAN2B2
Alt. symbolsKIAA0935
Entrez23324
HUGO29623
RefSeqNM_015274
UniProtQ9Y2E5
Other data
EC number3.2.1.24
LocusChr. 4 p16.2
mannosidase, alpha, class 2C, member 1
Identifiers
SymbolMAN2C1
Alt. symbolsMANA1, MANA
Entrez4123
HUGO6827
OMIM154580
RefSeqNM_006715
UniProtQ9NTJ4
Other data
EC number3.2.1.24
LocusChr. 15 q11-qter

Applications

It can be utilized in experiments that determine the effects of the presence or absence of mannose on specific molecules, such as recombinant proteins that are used in vaccine development.[5]

Pathology

A deficiency can lead to alpha-mannosidosis.[6]

References

  1. "PyMol". Schrodinger. Retrieved 2011-09-14.
  2. Suits, MDL; Yanping Zhu; Edward J. Taylor; Julia Walton; David L. Zechel; Harry J. Gilbert; Gideon J. Davies (3 February 2010). "Structure and Kinetic Investigation of Streptococcus pyogenes Family GH38 α-Mannosidase". PLoS ONE. 5 (2). doi:10.1371/journal.pone.0009006. Retrieved 2011-09-14.
  3. Li, Y.-T. (1966). "Presence of α-D-mannosidic linkage in glycoproteins. Liberation of D-mannose from various glycoproteins by α-mannosidase isolated from jack bean meal". J. Biol. Chem. 241 (4): 1010–1012. PMID 5905120.
  4. Winchester, B. (1984). "Role of α-D-mannosidases in the biosynthesis and catabolism of glycoproteins". Biochem. Soc. Trans. 12 (3): 522–524. PMID 6428944.
  5. Vlahopoulos S, Gritzapis AD, Perez SA, Cacoullos N, Papamichail M, Baxevanis CN (2009). "Mannose addition by yeast Pichia Pastoris on recombinant HER-2 protein inhibits recognition by the monoclonal antibody herceptin". Vaccine. 27 (34): 4704–8. doi:10.1016/j.vaccine.2009.05.063. PMID 19520203.
  6. Malm D, Nilssen Ø (2008). "Alpha-mannosidosis". Orphanet J Rare Dis. 3: 21. doi:10.1186/1750-1172-3-21. PMC 2515294. PMID 18651971.

External links


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