Aft1p gene transcriptions

Associate Editor(s)-in-Chief: Henry A. Hoff

Aft1 is Affinity Capture-Western; Two-hybrid.^[1]

Consensus sequences

The upstream activating sequence (UAS) for Aft1p is PyPuCACCCPu or (C/T)(A/G)CACCC(A/G).^[2]

Hypotheses

A1BG has no Aft1p in either promoter.
A1BG is not transcribed by an Aft1p.
Aft1p does not participate in the transcription of A1BG.

AFT1 samplings

Copying TGCACCC in "⌘F" yields none between ZSCAN22 and A1BG and one TGCACCCG between ZNF497 and A1BG as can be found by the computer programs.

For the Basic programs testing consensus sequence (C/T)(A/G)CACCC(A/G) (starting with SuccessablesAFT.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:

negative strand, negative direction, looking for (C/T)(A/G)CACCC(A/G), 0.
negative strand, positive direction, looking for (C/T)(A/G)CACCC(A/G), 1, TGCACCCG at 3324.
positive strand, negative direction, looking for (C/T)(A/G)CACCC(A/G), 0.
positive strand, positive direction, looking for (C/T)(A/G)CACCC(A/G), 0.
inverse complement, negative strand, negative direction, looking for (C/T)GGGTG(C/T)(A/G), 1, TGGGTGTG at 1804.
inverse complement, negative strand, positive direction, looking for (C/T)GGGTG(C/T)(A/G), 0.
inverse complement, positive strand, negative direction, looking for (C/T)GGGTG(C/T)(A/G), 3, TGGGTGTG at 3185, CGGGTGCG at 2196, CGGGTGCG at 379.
inverse complement, positive strand, positive direction, looking for (C/T)GGGTG(C/T)(A/G), 0.

AFT UTRs

Positive strand, negative direction: TGGGTGTG at 3185.

AFT distal promoters

Negative strand, negative direction: TGGGTGTG at 1804.
Positive strand, negative direction: CGGGTGCG at 2196, CGGGTGCG at 379.

Negative strand, positive direction: TGCACCCG at 3324.

AFT random dataset samplings

AFTr0: 0.
AFTr1: 1, CGCACCCA at 237.
AFTr2: 0.
AFTr3: 0.
AFTr4: 0.
AFTr5: 0.
AFTr6: 0.
AFTr7: 0.
AFTr8: 1, CACACCCG at 1053.
AFTr9: 0.
AFTr0ci: 1, TGGGTGCG at 643.
AFTr1ci: 2, TGGGTGTA at 4170, TGGGTGTA at 2489.
AFTr2ci: 1, TGGGTGCA at 2005.
AFTr3ci: 0.
AFTr4ci: 0.
AFTr5ci: 1, TGGGTGTA at 2219.
AFTr6ci: 0.
AFTr7ci: 0.
AFTr8ci: 0.
AFTr9ci: 1, TGGGTGCG at 3727.

AFTr proximal promoters

AFTr1ci: TGGGTGTA at 4170.

AFTr distal promoters

AFTr8: CACACCCG at 1053.
AFTr0ci: TGGGTGCG at 643.
AFTr2ci: TGGGTGCA at 2005.

AFTr1: CGCACCCA at 237.
AFTr1ci: TGGGTGTA at 2489.
AFTr5ci: TGGGTGTA at 2219.
AFTr9ci: 1, TGGGTGCG at 3727.

Acknowledgements

The content on this page was first contributed by: Henry A. Hoff.

References

↑ RefSeq (18 September 2021). "AFT1 DNA-binding transcription factor AFT1 [ Saccharomyces cerevisiae S288C ]". 8600 Rockville Pike, Bethesda MD, 20894 USA: National Center for Biotechnology Information, U.S. National Library of Medicine. Retrieved 19 October 2021.
↑ Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check |pmid= value (help). Retrieved 18 September 2020.

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