Ndt80p gene transcriptions: Difference between revisions
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# inverse negative strand, positive direction, looking for (A/T)AAAC(A/G)CN(A/G/T), 0. | # inverse negative strand, positive direction, looking for (A/T)AAAC(A/G)CN(A/G/T), 0. | ||
===NDT | ===NDT (4560-2846) UTRs=== | ||
Positive strand, negative direction: ACCACAAAA at 3767. | # Negative strand, negative direction: TTTTGTGTT at 3514. | ||
# Positive strand, negative direction: ACCACAAAA at 3767. | |||
==See also== | ==See also== | ||
Revision as of 14:56, 14 August 2022
Associate Editor(s)-in-Chief: Henry A. Hoff
"The Saccharomyces cerevisiae Ndt80 protein is the founding member of a class of p53-like transcription factors that is known as the NDT80/PhoG-like DNA-binding family."[1]
Ndt80 [Non-DiTyrosine 80] is a meiosis-specific transcription factor required for successful completion of meiosis and spore formation.[2] The DNA-binding domain of Ndt80 has been isolated, and the structure reveals that this protein is a member of the Ig-fold family of transcription factors.[3] Ndt80 also competes with the repressor SUM1 for binding to promoters containing MSEs.[4]
Direct "binding of Ndt80 to the [mid sporulation element CRCAAAA/T (Ozsarac et al. 1997)] MSE is necessary for activating transcription of the middle sporulation genes."[5]
"These genomic sequences were analysed for the presence of [...] middle sporulation elements (MSE) motif (5'-ACACAAA-3') using the NCBI BLAST tool."[6]
Human genes
Interactions
Consensus sequences
The upstream activating sequence (UAS) for the Ndt80p is 5'-DNCRCAAAW-3'.[7]
Ndt80 samplings
Copying DNCRCAAAW, (A/G/T)(A/C/G/T)C(A/G)CAAA(A/T), or TCCGCA in "⌘F" yields none between ZSCAN22 and A1BG and none between ZNF497 and A1BG as can be found by the computer programs.
For the Basic programs testing consensus sequence (A/G/T)(A/C/G/T)C(A/G)CAAA(A/T) (starting with SuccessablesNDT.bas) written to compare nucleotide sequences with the sequences on either the template strand (-), or coding strand (+), of the DNA, in the negative direction (-), or the positive direction (+), the programs are, are looking for, and found:
- negative strand, negative direction, looking for (A/G/T)NC(A/G)CAAA(A/T), 0.
- positive strand, negative direction, looking for (A/G/T)NC(A/G)CAAA(A/T), 1, ACCACAAAA at 3767.
- positive strand, positive direction, looking for (A/G/T)NC(A/G)CAAA(A/T), 0.
- negative strand, positive direction, looking for (A/G/T)NC(A/G)CAAA(A/T), 0.
- complement, negative strand, negative direction, looking for (A/C/T)NG(C/T)GTTT(A/T), 1, TGGTGTTTT at 3767.
- complement, positive strand, negative direction, looking for (A/C/T)NG(C/T)GTTT(A/T), 0.
- complement, positive strand, positive direction, looking for (A/C/T)NG(C/T)GTTT(A/T), 0.
- complement, negative strand, positive direction, looking for (A/C/T)NG(C/T)GTTT(A/T), 0.
- inverse complement, negative strand, negative direction, looking for (A/T)TTTG(C/T)GN(A/C/T), 1, TTTTGTGTT at 3514.
- inverse complement, positive strand, negative direction, looking for (A/T)TTTG(C/T)GN(A/C/T), 0.
- inverse complement, positive strand, positive direction, looking for (A/T)TTTG(C/T)GN(A/C/T), 0.
- inverse complement, negative strand, positive direction, looking for (A/T)TTTG(C/T)GN(A/C/T), 0.
- inverse negative strand, negative direction, looking for (A/T)AAAC(A/G)CN(A/G/T), 0.
- inverse positive strand, negative direction, looking for (A/T)AAAC(A/G)CN(A/G/T), 1, AAAACACAA at 3514.
- inverse positive strand, positive direction, looking for (A/T)AAAC(A/G)CN(A/G/T), 0.
- inverse negative strand, positive direction, looking for (A/T)AAAC(A/G)CN(A/G/T), 0.
NDT (4560-2846) UTRs
- Negative strand, negative direction: TTTTGTGTT at 3514.
- Positive strand, negative direction: ACCACAAAA at 3767.
See also
References
- ↑ Margaret E Katz and Sarah Cooper (1 December 2015). "Extreme Diversity in the Regulation of Ndt80-Like Transcription Factors in Fungi". G3 Genes. 5 (12): 2783–2792. doi:10.1534/g3.115.021378. Retrieved 8 February 2021. Text "Genomes" ignored (help); Text "Genetics " ignored (help)
- ↑ Xu L, Ajimura M, Padmore R, Klein C, Kleckner N (December 1995). "NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae". Molecular and Cellular Biology. 15 (12): 6572–81. doi:10.1128/MCB.15.12.6572. PMC 230910. PMID 8524222.
- ↑ Lamoureux JS, Stuart D, Tsang R, Wu C, Glover JN (November 2002). "Structure of the sporulation-specific transcription factor Ndt80 bound to DNA". The EMBO Journal. 21 (21): 5721–32. doi:10.1093/emboj/cdf572. PMC 131069. PMID 12411490.
- ↑ Hendrickson WA, Ward KB (October 1975). "Atomic models for the polypeptide backbones of myohemerythrin and hemerythrin". Biochemical and Biophysical Research Communications. 66 (4): 1349–56. doi:10.1016/0006-291x(75)90508-2. PMID 5.
- ↑ Shelley Chu and Ira Herskowitz (April 1, 1998). "Gametogenesis in Yeast Is Regulated by a Transcriptional Cascade Dependent on Ndt80". Molecular Cell. 1 (5): 685–96. doi:10.1016/S1097-2765(00)80068-4. PMID 9660952.
- ↑ J. Branco, M. Ola, R. M. Silva, E. Fonseca, N. C. Gomes, C. Martins-Cruz, A. P. Silva, A. Silva-Dias, C. Pina-Vaz, C. Erraught, L. Brennan, A. G. Rodrigues, G. Butler and I. M. Miranda (August 2017). "Impact of ERG3 mutations and expression of ergosterol genes controlled by UPC2 and NDT80 in Candida parapsilosis azole resistance". Clinical Microbiology and Infection. 23 (8): 575.e1–575.e8. doi:10.1016/j.cmi.2017.02.002. PMID 28196695. Retrieved 5 September 2020.
- ↑ Hongting Tang, Yanling Wu, Jiliang Deng, Nanzhu Chen, Zhaohui Zheng, Yongjun Wei, Xiaozhou Luo, and Jay D. Keasling (6 August 2020). "Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae". Metabolites. 10 (8): 320–39. doi:10.3390/metabo10080320. PMID 32781665 Check
|pmid=value (help). Retrieved 18 September 2020.