Anthrax pathophysiology: Difference between revisions

	Anthrax Microchapters
Home
Patient Information
Overview
Historical Perspective
Pathophysiology
Causes
Differentiating Anthrax from other Diseases
Epidemiology and Demographics
Risk Factors
Natural History, Complications and Prognosis
Diagnosis
History and Symptoms
Physical Examination
Laboratory Findings
Chest X Ray
CT
Other Diagnostic Studies
Treatment
Medical Therapy
Surgery
Prevention
Cost-Effectiveness of Therapy
Future or Investigational Therapies
Case Studies
Case #1
Anthrax pathophysiology On the Web
Most recent articles
cited articles
Review articles
CME Programs
Powerpoint slides
Images
American Roentgen Ray Society Images of Anthrax pathophysiology All Images X-rays Echo & Ultrasound CT Images MRI
Ongoing Trials at Clinical Trials.gov
US National Guidelines Clearinghouse
NICE Guidance
FDA on Anthrax pathophysiology
CDC on Anthrax pathophysiology
Anthrax pathophysiology in the news
Blogs on Anthrax pathophysiology
Directions to Hospitals Treating Anthrax
Risk calculators and risk factors for Anthrax pathophysiology

VisualWikitext

Latest revision as of 20:25, 29 July 2020

Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: João André Alves Silva, M.D. [2]

Overview

The genetic material of Bacillus anthracis is coded within 1 chromosome and 2 plasmids, which are fundamental for its toxicity. The spores of B. anthracis are the infectious form and can remain dormant in the environment for decades. The disease may be transmitted through the skin, gastrointestinal or respiratory systems. The bacterium causes disease through 2 mechanisms: toxemia and bacterial infection.^[1] B. anthracis begins to produce toxins within hours of germination.^[2] Protective antigen (PA) and edema factor (EF) combine to form edema toxin (ET), and PA and lethal factor (LF) combine to form lethal toxin (LT), the active toxins. Bacterial toxins have a direct cytotoxic effect by interfering with cellular pathways, being also responsible for weakening the immune system, so the initial systemic infection may occur. Anthrax lesions at any site are characterized by lymphadenopathy, extensive edema, necrosis and confluent exudate containing macrophages and neutrophils. If not stopped, the infection may affect different organs, causing septicemia and potentially death.

Genetics

The genetic component of Bacillus anthracis includes 1 chromosome and 2 plasmids. These plasmids (pXO1 and pXO2) are fundamental for its toxicity:^[1]

pXO1 - encodes 3 components of the anthrax exotoxins:

Protective Antigen (PA)
Lethal Factor (LF)
Edema Factor (EF)

pXO2 - encodes proteins involved in the glutamic acid capsule structure which is responsible for conferring resistance against phagocytosis.

Transmission

The route of transmission of anthrax allows for its classification into the following:^[3]

Cutaneous anthrax - commonly requires a prior skin lesion as a prerequisite for infection
Gastrointestinal anthrax - contracted following ingestion of contaminated food, primarily meat from an animal that died of the disease, or conceivably from ingestion of contaminated water
Inhalational anthrax - from breathing in airborne anthrax spores
Injection anthrax - from the injection of a drug containing or contaminated with Bacillus anthracis

Pathogenesis

B. anthracis, the causative agent of anthrax, is a spore-forming bacterium. The spores of B. anthracis, which can remain dormant in the environment for decades, are the infectious form, but this vegetative form of B. anthracis rarely causes disease.^[4] The bacterium causes disease through 2 mechanisms: toxemia and bacterial infection.^[1] Spores introduced through the skin lead to cutaneous or injection anthrax; those introduced through the gastrointestinal tract lead to gastrointestinal anthrax; and those introduced through the lungs lead to inhalation anthrax. After entering a human or animal, B. anthracis spores are believed to germinate locally or be phagocytosed by dendritic cells and macrophages. These will then carry the spores to the lymph nodes, where they germinate.^[5]^[1] B. anthracis begins to produce toxins within hours of germination.^[2] Protective antigen (PA) and edema factor (EF) combine to form edema toxin (ET), and PA and lethal factor (LF) combine to form lethal toxin (LT). After binding to surface receptors, the PA portion of the complexes facilitates translocation of the toxins to the cytosol, in which EF and LF exert their toxic effects.^[6] Bacillus anthracis disseminate to multiple organs including spleen, liver, intestines, kidneys, adrenal glands, and meninges, affecting their normal functions and leading to systemic infection with a potentially fatal outcome.^[7]^[8]^[3]

The virulence factors of Bacillus anthracis are:

PA
LF
EF

Bacterial Toxins

In order to infect the body, Bacillus anthracis must produce toxins. These toxins have 3 main toxic effects: edema, hemorrhage, and necrosis. Besides their direct toxic effects responsible for tissue damage, anthrax toxins are also responsible for interfering with cellular pathways, in such way that defense functions of the host's immune system are affected. This will ultimately allow initial systemic infection by interfering with the immune system.^[1]

When isolated, the 3 structural elements of the anthrax exotoxins are non-toxic. However, when combined, they form virulent exotoxins:^[1]

LF + PA = LT (Lethal Toxin)
EF + PA = ET (Edema Toxin)

The PA is responsible for attaching the toxin to the cell, while the LF and the EF are responsible for the toxicity.^[1]

After germinating, B. anthracis produces and releases into the blood stream PA, LF, and EF toxins separately. However, PA is secreted in its inactivated form (PA). In order to form the exotoxin complexes with LF and EF, it must first be activated by host-cellular receptors:^[1]

CMG2 - Capillary Morphogenesis Protein 2 (predominant toxin receptorin vivo)
TEM8 - Tumor Endothelium Marker 8 (minor role)

CMG2 and TEM8 cleave PA into PA20 and PA63. PA63 (a C-terminal fragment) is the activated form of PA, responsible for combining with EF and LF, thereby creating the toxin oligomer PA63 oligomer receptor complex. This complex will be internalized via receptor mediated endocytosis within an endosome.^[1]

The acidic environment within the endosomes leads to the formation of a channel called PA63 oligomer channel, on the endosomal membrane. LF and EF are then released in the cytosol of the host cell, to then exert their toxic effects.^[1]

After experiments in mice, edema toxin was noted to be the major virulence factor since it caused death of mice in much lesser dosages than lethal toxin.

Edema toxin is a calmodulin-dependent adenylyl cyclase, known to increase intracellular cAMP through the conversion of ATP into cAMP, thus affecting several intracellular pathways.
Lethal toxin is a zinc-dependent metaloproteinase known to interfere with the mitogen-activated protein kinase (MEK), thereby hampering multiple intracellular mechanisms.^[1]

Cutaneous or Injection Anthrax

According to animal studies, spores that enter the skin of susceptible animals (either through a lesion or by injection) germinate and give rise, in about 2 - 4 hours, to a small edematous area containing capsulated bacilli. The following stages are noticed:^[3]

The lesion increases in size and becomes defined by a zone of altered capillaries through dilatation, congestion, and swelling of the endothelial cells and through diapedesis of neutrophils.
Macrophages and fibrin deposits appear, and the efferent lymphatics become dilated.
Continued multiplication of the bacilli in the lesion leads to fragmentation of the connective tissue, as the edema spreads extensively away from the infection site.
Necrosis and hemorrhage occur. If initial inoculation has happened with minimal number of spores, the primary lesion may be insignificant compared with that of the draining lymph nodes.
The bacilli migrate from the primary lesion, by means of the lymph vessels, to the local lymph nodes where multiplication occurs, releasing a continuous stream of organisms and toxins into the efferent lymph vessels and to the spleen and other lymphoid tissues where multiplication continues.
In animals who died following subcutaneous challenge, the local lymph nodes became haemorrhagic and edematous. Necrosis of lymphatic elements and of blood vessel walls occur and phagocytosis of the bacilli is apparent.
Septicemia often courses with splenic enlargement

Injection anthrax will have similar pathogenesis to cutaneous anthrax, but since it is injected, it can spread throughout the body faster and it becomes harder to recognize and treat than the cutaneous form.^[9]

Inhalation Anthrax

In inhalation anthrax, the inhaled spores will be deposited in the alveoli first. From there, they will be transported, within phagocytic cells, through the lymphatic vessels to the mediastinal lymph nodes, where they will grow and cause hemorrhagic lymphadenitis. Bacteria escape from the damaged lymph nodes and invade the blood stream via the thoracic duct. Vegetative Bacillus then travel through the bloodstream and lymph vessels, potentially causing septicemia. At the same time toxins are released, causing tissue damage and hampering the immune system to facilitate bacterial spread.^[10]^[11]^[12]

Once the bacteremia and associated toxemia reach a critical level, the severe symptoms that are characteristic of the acute phase of illness are manifested. During the acute phase, damage of the lung tissue becomes apparent on the X-ray. This damage results from the action of anthrax toxin on the endothelium of the lung’s capillary bed. Primary damage of the lung is not normally a feature of the initial phase of illness and primary pulmonary infection is an uncommon presentation.^[13]^[11]^[12]

Studies in rhesus monkeys revealed that after spore inhalation, its germination might take up to 60 days. This is the reason why antibiotic prophylaxis is recommended for 60 days.^[11]

Gastrointestinal Anthrax

In animal studies, the intestinal lesions caused by ingested anthrax spores range from focal to diffuse hemorrhagic necrotic enteritis of the small intestine. The tendency for localized lesions to develop in Peyer's patches suggests a possible role of the M cell in the uptake of the anthrax bacillus.^[3]

Gross Pathology

Cutaneous and Injection Anthrax

Cutaneous infection typically produces ulcerated lesions which are covered by a scab and often contain numerous microorganisms. Anthrax eschars are generally seen on exposed unprotected regions of the body, mostly on the face, neck, hands and wrists. Generally cutaneous lesions are single, but sometimes two or more lesions are present.^[14]^[15]

The lesions produced by injection anthrax will be similar to the ones of the cutaneous form. The difference will reside on the fact that injection anthrax can spread throughout the body faster and be harder to recognize and treat than cutaneous anthrax.^[9]

Inhalational Anthrax

Gross pathologic lesions observed in non-human primates used in aerosol challenge models of inhalation anthrax include edema, congestion, hemorrhage, and necrosis in the lungs and mediastinum. Splenitis and necrotizing or hemorrhagic lymphadenitis involving the mediastinal, tracheobronchial, and other lymph nodes are common.^[16] Primary pulmonary lesions, including those of pneumonia, are occasionally observed. Meningeal involvement ranging from edema, congestion, hemorrhage, and necrosis to suppurative or hemorrhagic meningitis, usually secondary to hematogenous spread from other types of anthrax, occurs in ≤77% of animals studied.^[17] Autopsy findings from persons who died from inhalation anthrax in Sverdlovsk and in the United States^[18] are consistent with findings from the non-human primates studies. Persons who died had extensive amounts of serosanguinous fluid in pleural cavities, edema, and hemorrhage of the mediastinum and surrounding soft tissues. 48% had cerebral edema, 21% had ascites, 17% had pericardial effusions, and 14% had petechial rash. Mediastinal lymph nodes and spleen also showed hemorrhage and necrosis.^[16]^[19]

Gastrointestinal Anthrax

On gastrointestinal infection the typical eschar may occur on different locations, including:^[11]

According to the location of the eschar, gastrointestinal anthrax may be divided in 2 categories: oropharyngeal and abdominal.^[11]

As the eschar progresses, symptoms will appear as a result of the necrosis of the lesion, coupled with severe intestinal and mesenteric edema and lymph node enlargement in the mesentery.^[11]

Microscopic Pathology

Anthrax lesions at any site are characterized by extensive necrosis and confluent exudate, containing macrophages and neutrophils. In histopathological specimens or culture media, the presence of large boxcar-shaped Gram-positive bacilli in chains suggests the diagnosis.

Cutaneous or Injection Anthrax

Histologic examination of skin lesions caused by cutaneous anthrax reveals:^[20]

Tissue necrosis
Edema
Lymphocytic infiltrates
No liquefaction
No abscess formation (lesion is not suppurative)
Focal points of hemorrhage
Thrombosis
Bacilli in subcutaneous tissue, revealed by Gram staining

Inhalation Anthrax

Histologic evaluation of affected tissues reveals:

Numerous necrotic foci of hemorrhage in the mediastinum or hilar and peribronchial lymph nodes
Pulmonary infection may also result in interstitial pneumonia associated with:

Vasculitis
Edema
Infiltrating macrophages and neutrophils

Bacilli are most likely to be observed in the alveolar capillaries and venules and, to a lesser degree, within the alveolar space and draining lymph nodes

Gastrointestinal Anthrax

Histologic evaluation of affected tissues revealed:^[20]

Edema
Mucosal necrosis
Inflammatory infiltrates
Peritoneal fluid may reveal gram-positive bacilli
Occasionally mediastinal widening

Gallery

Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Sterne strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on sheep blood agar (SBA) medium, for a 48 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Sterne strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on sheep blood agar (SBA) medium, for a 24 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 5X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Sterne strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on sheep blood agar (SBA) medium, for a 24 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Sterne strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on polymyxin B - lysozyme - EDTA – thallous acetate agar (PLET) medium, for a 24 hour time period, at a temperature of 37°C”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Sterne strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on phenylethyl alcohol agar (PEA) medium, for a 48 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Sterne strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on phenylethyl alcohol agar (PEA) medium, for a 24 hour time period, at a temperature of 37°.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 5X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Sterne strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on phenylethyl alcohol agar (PEA) medium, for a 24 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Sterne strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on mannitol, egg yolk, polymyxin agar (MEP) medium, for a 24 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 5X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Sterne strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on mannitol, egg yolk, polymyxin agar (MEP) medium, for a 24 hour time period, at a temperature of 37°C. ”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Sterne strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on colistin-naladixic acid agar (CNA) medium, for a 48 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Sterne strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on colistin-naladixic acid agar (CNA) medium, for a 24 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Sterne strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on mannitol, egg yolk, polymyxin agar (MEP) medium, for a 48 hour time period, at a temperature of 37°C. ”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Pasteur strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on polymyxin B - lysozyme - EDTA – thallous acetate agar (PLET) medium, for a 48 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Pasteur strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on sheep blood agar (SBA) medium, for a 48 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Pasteur strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on sheep blood agar (SBA) medium, for a 24 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 5X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Pasteur strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on sheep blood agar (SBA) medium, for a 24 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Pasteur strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on polymyxin B - lysozyme - EDTA – thallous acetate agar (PLET) medium, for a 48 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Pasteur strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on polymyxin B - lysozyme - EDTA – thallous acetate agar (PLET) medium, for a 24 hour time period, at a temperature of 37°C. ”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Pasteur strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on phenylethyl alcohol agar (PEA) medium, for a 48 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Pasteur strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on phenylethyl alcohol agar (PEA) medium, for a 48 hour time period, at a temperature of 37°C. ”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Pasteur strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on phenylethyl alcohol agar (PEA) medium, for a 24 hour time period, at a temperature of 37°C. ”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Pasteur strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on phenylethyl alcohol agar (PEA) medium, for a 24 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Pasteur strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on mannitol, egg yolk, polymyxin agar (MEP) medium, for a 48 hour time period, at a temperature of 37°C. ”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Pasteur strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on colistin-naladixic acid agar (CNA) medium, for a 48 hour time period, at a temperature of 37°C. ”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Pasteur strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on colistin-naladixic acid agar (CNA) medium, for a 24 hour time period, at a temperature of 37°C. ”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Pasteur strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on colistin-naladixic acid agar (CNA) medium, for a 24 hour time period, at a temperature of 37°C. ”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Magnification of 10X, this image depicts a number of Gram-positive Bacillus anthracis bacterial colonies of the Sterne-strain, which had been cultivated on a blood agar plate (BAP), and incubated for a 24 hour time period at a temperature of 35 °C. Of particular note was the classic “ground glass” texture exhibited by the colonies, resembling a pile of glass shards. As they continued to grow, and their edges touched, you can see that these colonies had coalesced, destroying their originally well-demarcated borders.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Magnification of 10X, this image depicts a number of Gram-positive Bacillus anthracis bacterial colonies of the Sterne-strain, which had been cultivated on a blood agar plate (BAP), and incubated for a 24 hour time period at a temperature of 35 °C. Of particular note was the classic “ground glass” texture exhibited by the colonies, resembling a pile of glass shards. As they continued to grow, and their edges touched, you can see that these colonies had coalesced, destroying their originally well-demarcated borders.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Magnification of 30X, this image depicts a single Gram-positive Bacillus anthracis bacterial colony of the Sterne-strain, which had been cultivated on a blood agar plate (BAP), and incubated for a 24 hour time period at a temperature of 35 °C. Of particular note was the classic “ground glass” texture exhibited by the colony, resembling a pile of glass shards. In the background, you’ll note that as these colonies continued to grow, and their edges touched, they coalesced, destroying their originally well-demarcated borders.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Magnification of 20X, this image depicts a number of Gram-positive Bacillus anthracis bacterial colonies of the Sterne-strain, which had been cultivated on a blood agar plate (BAP), and incubated for a 24 hour time period at a temperature of 35 °C. Of particular note was the classic “ground glass” texture exhibited by the colonies, resembling a pile of glass shards. As they continued to grow, and their edges touched, you can see that these colonies had coalesced, destroying their originally well-demarcated borders.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Hematoxylin-eosin-stained (H&E) photomicrograph of a tissue specimen harvested from the bronchus of a deceased 60 year-old man, revealed cytoarchitectural changes associated with a case of inhalation anthrax, due to Gram-positive Bacillus anthracis bacteria.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Colonial morphology displayed by Gram-positive, Pasteur strain, Bacillus anthracis bacteria, which was grown on a medium of phenylethyl alcohol agar (PEA), for a 24 hour time period, at a temperature of 37°C”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Colonial morphology displayed by Gram-positive, Pasteur strain, Bacillus anthracis bacteria, which was grown on a medium of phenylethyl alcohol agar (PEA), for a 24 hour time period, at a temperature of 37°C. ”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Light colonial growth displayed by Gram-positive, Pasteur strain, Bacillus anthracis bacteria, which was grown on a medium of MacConkey agar, for a 24 hour time period, at a temperature of 37°C. ”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Light colonial growth displayed by Gram-positive, Pasteur strain, Bacillus anthracis bacteria, which was grown on a medium of MacConkey agar, for a 24 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Light colonial growth displayed by Gram-positive, Pasteur strain, Bacillus anthracis bacteria, which was grown on a medium of CNA agar, for a 24 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Light colonial growth displayed by Gram-positive, Pasteur strain, Bacillus anthracis bacteria, which was grown on a medium of CNA agar, for a 24 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Colonial morphology displayed by Gram-positive, Pasteur strain, Bacillus anthracis bacteria, which was grown on a medium of chocolate agar, for a 24 hour time period, at a temperature of 37°C.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Bacillus anthracis bacterial colonies, which had been allowed to grow on sheep’s blood agar (SBA) for a 24 hour period. In this particular view you’ll note the appearance of what is termed a "plaque" (arrowhead), which represents an area where the bacteria had been lysed, or destroyed by the application of a localized amount of gamma phage-containing solution. Highly specific toB. anthracis, these gamma phage viruses, i.e., bacteriophages, attacked the B. anthracis bacteria, subsequently leaving this circular plaque devoid of bacterial organisms. The specificity of these gamma phages to B. anthracis makes this a positive test for the presence of this bacterium.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Bacillus anthracis bacterial colonies, which had been allowed to grow on sheep’s blood agar (SBA) for a 24 hour period. Note the classical appearance exhibited in the colonial morphology including a ground-glass, non-pigmented texture with accompanying “comma” projections from some of the individual rough-edged colonies. In this particular view, you’ll note that a “tenacity test” had been performed using an iinoculating loop, which proved positive for B. anthracis, causing the “colony to ‘stand up’ like beaten egg white”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Bacillus anthracis bacterial colonies, which have been allowed to grow on sheep’s blood agar (SBA) for a 24 hour period. Note the classical appearance exhibited in the colonial morphology including a ground-glass, non-pigmented texture with accompanying “comma” projections from some of the individual rough-edged colonies. In this particular view, you’ll note that a “tenacity test” had been performed using an iinoculating loop, which proved positive for B. anthracis, causing the “colony to ‘stand up’ like beaten egg white”.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Bacillus anthracis bacterial colonies, which have been allowed to grow on sheep’s blood agar (SBA) for a 24 hour period. Note the classical appearance exhibited in the colonial morphology including a ground-glass, non-pigmented texture with accompanying “comma” projections from some of the individual rough-edged colonies. In this particular view, you’ll note that a “tenacity test” had been performed using an iinoculating loop, which proved positive for B. anthracis, causing the “colony to ‘stand up’ like beaten egg white”.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Bacillus anthracis bacterial colonies, which have been allowed to grow on sheep’s blood agar (SBA) for a 24 hour period. Note the classical appearance exhibited in the colonial morphology including a ground-glass, non-pigmented texture with accompanying “comma” projections from some of the individual rough-edged colonies.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Bacillus anthracis bacterial colonies, which have been allowed to grow on sheep’s blood agar (SBA) for a 24 hour period. Note the classical appearance exhibited in the colonial morphology including a ground-glass, non-pigmented texture with accompanying “comma” projections from some of the individual rough-edged colonies. See PHIL 11748 for a higher magnification of these colonies.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Magnification of 6,408X, this scanning electron micrograph (SEM) depicted spores from the Aimes strain of Bacillus anthracis bacteria. ”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Magnification of 31,207X, this scanning electron micrograph (SEM) depicted spores from the Sterne strain of Bacillus anthracis bacteria. ”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Magnification of 12,483X, this scanning electron micrograph (SEM) depicted spores from the Sterne strain of Bacillus anthracis bacteria.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Gram-positive, endospore-forming Bacillus anthracisbacteria. B. anthracis is the pathologic microorganism responsible for the disease “anthrax”, an acute infectious disease, which most commonly occurs in wild and domestic vertebrates (cattle, sheep, goats, camels, antelopes, and other herbivores), but it can also occur in humans when they are exposed to infected animals, or tissue from infected animals.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Gross pathologic posterior oblique view of a chimpanzee's lungs that had contracted fatal inhalation anthrax.” Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Micrograph revealing submucosal hemorrhage in the small intestine, in a case of fatal human anthrax; H&E stain; Mg. 240X.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Micrograph revealing submucosal hemorrhage in the small intestine, in a case of fatal human anthrax; H&E stain; Mg. 240X.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Meningeal infection due to Bacillus anthracis bacteria using an H&E stain” Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Blood agar culture plate growing B. anthracis and other soil flora.” Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Brain section through the ventricles revealing an inter ventricular hemorrhage as consequence of virulence factors of Bacillus anthracis (edema toxin, lethal toxin, and antiphagocytic capsular antigen). The toxins are responsible for the primary clinical manifestations of hemorrhage, edema, and necrosis”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Agar culture plate is growing Bacillus anthracis colonies.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Blood agar culture plate growing B. anthracis and other soil flora.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
Cutaneous anthrax lesion on the arm of a 50 year old female.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Magnification of 12,483X, this scanning electron micrograph (SEM) depicted spores from the Sterne strain of Bacillus anthracis bacteria. ”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Magnification of 12,483X, this scanning electron micrograph (SEM) depicted spores from the Sterne strain of Bacillus anthracis bacteria.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Magnification of 6,408X, this scanning electron micrograph (SEM) depicted spores from the Aimes strain of Bacillus anthracis bacteria.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Photomicrograph of Bacillus anthracis bacteria using Gram-stain technique”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis taken from the peritoneum using a Hiss capsule stain.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Photomicrograph of Bacillus anthracis bacteria taken from heart blood, and using Carbol Fuchsin stain.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis positive encapsulation test is demonstrated using two different agar media”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Photomicrograph demonstrating a positive Gram stain with Bacillus anthracis”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis tenacity positive on sheep blood agar.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis tenacity positive on sheep blood agar”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis tenacity positive on sheep blood agar”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis Avirulent Pasteur Strain, Non-hemolytic on sheep blood agar” Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis spores seen under phase contrast microscopy”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis spores seen under phase contrast microscopy”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis spores seen under phase contrast microscopy”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis spores seen under phase contrast microscopy”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"B. anthracis Direct Fluorescent Antibody (DFA) capsule stain”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"B. anthracis Direct Fluorescent Antibody (DFA) capsule stain.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"B. anthracis Direct Fluorescent Antibody (DFA) capsule stain.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"B. anthracis Direct Fluorescent Antibody (DFA) cell wall stain.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"B. anthracis Direct Fluorescent Antibody (DFA) cell wall stain.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis gamma phage lysis on sheep blood agar.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis Indian Ink capsule stain.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis M'Fadyean capsule stain”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis capsule production on bicarbonate agar medium”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Hemorrhagic lymph node due to inhalation anthrax”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Transmission electron micrograph of Bacillus anthracis.” Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Transmission electron micrograph of Bacillus anthracis”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Transmission electron micrograph of Bacillus anthracis”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Transmission electron micrograph of Bacillus anthracis.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Transmission electron micrographic image of Bacillus anthracis”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Transmission electron micrograph of Bacillus anthracis”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Confocal micrograph of Bacillus anthracis”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Numerous rod-shaped Bacillus anthracis bacteria, some that had linked together to form chains, while others remained solitary”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Necrosis of lymph node due to anthrax”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis from agar culture Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Photomicrograph of meninges demonstrating the presence of Bacillus anthracis in a case of fatal inhalation anthrax.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Photomicrograph of lung tissue demonstrating pneumonia in a case of fatal human inhalation anthraxAdapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Bacillus anthracis in lung Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Meningeal hemorrhage due to anthrax”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Photomicrograph of meninges demonstrating hemorrhage due to fatal inhalation anthrax”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Severe hemorrhagic necrosis of lymph node due to anthrax”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Mild meningitis with hemorrhage due to Bacillus anthracis”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Photomicrograph of serohemorrhagic meningitis in a case of fatal human anthrax with the presence ofBacillus anthracis.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Blood agar plate culture of Bacillus anthracis, with a positive gamma phage test”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Sheep blood agar plate culture of Bacillus anthracis and Bacillus cereus”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Gross pathology of fixed, cut brain showing hemorrhagic meningitis due to inhalation anthrax.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Gross pathology of fixed, cut brain showing hemorrhagic meningitis due to inhalation anthrax.”Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Gram-stained photomicrograph revealing numerous rod-shaped Bacillus anthracis bacteria that had linked together to form long filamentous chains.” Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Gram-stained photomicrograph revealing numerous rod-shaped Bacillus anthracis bacteria that had linked together to form long filamentous chains.” Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Hematoxylin-eosin (H&E)-stained photomicrograph of a small intestinal tissue sample revealed the presence of histopathologic changes indicative of marked mucosal and submucosal hemorrhage, with accompanying arteriolar degeneration in a case of fatal human anthrax.” Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Hematoxylin-eosin (H&E)-stained photomicrograph of a meningeal tissue sample revealed the presence of histopathologic changes indicative of hemorrhagic meningitis in a case of fatal human anthrax.” Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Histopathology of mediastinal lymph node in fatal human anthrax” Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Histopathology of mediastinal lymph node in fatal human anthrax” Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Hematoxylin-eosin (H&E)-stained photomicrograph of a mediastinal lymph node tissue sample revealed the presence of histopathologic changes indicative of an occluded blood vessel in a case of fatal human anthrax.” Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Hematoxylin-eosin (H&E)-stained photomicrograph of a mediastinal lymph node tissue sample revealed the presence of histopathologic changes indicative of medullary hemorrhage and necrosis in a case of fatal human anthrax.Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Hematoxylin-eosin (H&E)-stained photomicrograph of a mediastinal lymph node tissue sample revealed the presence of histopathologic changes indicative of medullary necrosis in a case of fatal human anthrax.” Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]
"Under a relatively-low magnification of 96X, this hematoxylin-eosin (H&E)-stained photomicrograph of a mediastinal lymph node tissue sample revealed the presence of histopathologic changes indicative of medullary necrosis in a case of fatal human anthrax.” Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.^[21]

References

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Liu, Shihui; Moayeri, Mahtab; Leppla, Stephen H. (2014). "Anthrax lethal and edema toxins in anthrax pathogenesis". Trends in Microbiology. 22 (6): 317–325. doi:10.1016/j.tim.2014.02.012. ISSN 0966-842X.
↑ ^2.0 ^2.1 Hanna, Philip C.; Ireland, John A.W. (1999). "Understanding Bacillus anthracis pathogenesis". Trends in Microbiology. 7 (5): 180–182. doi:10.1016/S0966-842X(99)01507-3. ISSN 0966-842X.
↑ ^3.0 ^3.1 ^3.2 ^3.3 "Anthrax in Humans and Animals" (PDF).
↑ Shadomy, Sean V.; Smith, Theresa L. (2008). "Anthrax". Journal of the American Veterinary Medical Association. 233 (1): 63–72. doi:10.2460/javma.233.1.63. ISSN 0003-1488.
↑ Ross, Joan M. (1957). "The pathogenesis of anthrax following the administration of spores by the respiratory route". The Journal of Pathology and Bacteriology. 73 (2): 485–494. doi:10.1002/path.1700730219. ISSN 0368-3494.
↑ Moayeri, M (2004). "The roles of anthrax toxin in pathogenesis". Current Opinion in Microbiology. 7 (1): 19–24. doi:10.1016/j.mib.2003.12.001. ISSN 1369-5274.
↑ Rubin, Raphael (2012). Rubin's pathology : clinicopathologic foundations of medicine. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins. ISBN 1605479683.
↑ Kumar, Vinay (2014). Robbins and Cotran pathologic basis of disease. Philadelphia, PA: Elsevier/Saunders. ISBN 0323266169.
↑ ^9.0 ^9.1 "Anthrax Symptoms".
↑ Turnbull, Peter (2008). Anthrax in humans and animals. Geneva, Switzerland: World Health Organization. ISBN 9789241547536.
↑ ^11.0 ^11.1 ^11.2 ^11.3 ^11.4 ^11.5 Spencer RC (2003). "Bacillus anthracis". J Clin Pathol. 56 (3): 182–7. PMC 1769905. PMID 12610093.CS1 maint: PMC format (link)
↑ ^12.0 ^12.1 Friedlander AM, Welkos SL, Pitt ML, Ezzell JW, Worsham PL, Rose KJ; et al. (1993). "Postexposure prophylaxis against experimental inhalation anthrax". J Infect Dis. 167 (5): 1239–43. PMID 8486963.
↑ Turnbull, Peter (2008). Anthrax in humans and animals. Geneva, Switzerland: World Health Organization. ISBN 9789241547536.
↑ Rubin, Raphael (2012). Rubin's pathology : clinicopathologic foundations of medicine. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins. ISBN 1605479683.
↑ Kumar, Vinay (2014). Robbins and Cotran pathologic basis of disease. Philadelphia, PA: Elsevier/Saunders. ISBN 0323266169.
↑ ^16.0 ^16.1 Guarner, Jeannette; Jernigan, John A.; Shieh, Wun-Ju; Tatti, Kathleen; Flannagan, Lisa M.; Stephens, David S.; Popovic, Tanja; Ashford, David A.; Perkins, Bradley A.; Zaki, Sherif R. (2003). "Pathology and Pathogenesis of Bioterrorism-Related Inhalational Anthrax". The American Journal of Pathology. 163 (2): 701–709. doi:10.1016/S0002-9440(10)63697-8. ISSN 0002-9440.
↑ Twenhafel, N. A. (2010). "Pathology of Inhalational Anthrax Animal Models". Veterinary Pathology. 47 (5): 819–830. doi:10.1177/0300985810378112. ISSN 0300-9858.
↑ A. A. Abramova & L. M. Grinberg (1993). "[Pathology of anthrax sepsis according to materials of the infectious outbreak in 1979 in Sverdlovsk (macroscopic changes)]". Arkhiv patologii. 55 (1): 12–17. PMID 7980032. Unknown parameter |month= ignored (help)
↑ A. A. Abramova & L. M. Grinberg (1993). "[Pathology of anthrax sepsis according to materials of the infectious outbreak in 1979 in Sverdlovsk (macroscopic changes)]". Arkhiv patologii. 55 (1): 12–17. PMID 7980032. Unknown parameter |month= ignored (help)
↑ ^20.0 ^20.1 Dixon, Terry C.; Meselson, Matthew; Guillemin, Jeanne; Hanna, Philip C. (1999). "Anthrax". New England Journal of Medicine. 341 (11): 815–826. doi:10.1056/NEJM199909093411107. ISSN 0028-4793.
↑ ^21.000 ^21.001 ^21.002 ^21.003 ^21.004 ^21.005 ^21.006 ^21.007 ^21.008 ^21.009 ^21.010 ^21.011 ^21.012 ^21.013 ^21.014 ^21.015 ^21.016 ^21.017 ^21.018 ^21.019 ^21.020 ^21.021 ^21.022 ^21.023 ^21.024 ^21.025 ^21.026 ^21.027 ^21.028 ^21.029 ^21.030 ^21.031 ^21.032 ^21.033 ^21.034 ^21.035 ^21.036 ^21.037 ^21.038 ^21.039 ^21.040 ^21.041 ^21.042 ^21.043 ^21.044 ^21.045 ^21.046 ^21.047 ^21.048 ^21.049 ^21.050 ^21.051 ^21.052 ^21.053 ^21.054 ^21.055 ^21.056 ^21.057 ^21.058 ^21.059 ^21.060 ^21.061 ^21.062 ^21.063 ^21.064 ^21.065 ^21.066 ^21.067 ^21.068 ^21.069 ^21.070 ^21.071 ^21.072 ^21.073 ^21.074 ^21.075 ^21.076 ^21.077 ^21.078 ^21.079 ^21.080 ^21.081 ^21.082 ^21.083 ^21.084 ^21.085 ^21.086 ^21.087 ^21.088 ^21.089 ^21.090 ^21.091 ^21.092 ^21.093 ^21.094 ^21.095 ^21.096 ^21.097 ^21.098 ^21.099 ^21.100 ^21.101 ^21.102 ^21.103 ^21.104 ^21.105 ^21.106 ^21.107 ^21.108 ^21.109 ^21.110 ^21.111 ^21.112 ^21.113 ^21.114 "Public Health Image Library (PHIL), Centers for Disease Control and Prevention".

Template:WikiDoc Sources

[LiuMoayeri2014-1] 1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Liu, Shihui; Moayeri, Mahtab; Leppla, Stephen H. (2014). "Anthrax lethal and edema toxins in anthrax pathogenesis". Trends in Microbiology. 22 (6): 317–325. doi:10.1016/j.tim.2014.02.012. ISSN 0966-842X.

[HannaIreland1999-2] 2.0 ^2.1 Hanna, Philip C.; Ireland, John A.W. (1999). "Understanding Bacillus anthracis pathogenesis". Trends in Microbiology. 7 (5): 180–182. doi:10.1016/S0966-842X(99)01507-3. ISSN 0966-842X.

[WHO-3] 3.0 ^3.1 ^3.2 ^3.3 "Anthrax in Humans and Animals" (PDF).

[ShadomySmith2008-4] Shadomy, Sean V.; Smith, Theresa L. (2008). "Anthrax". Journal of the American Veterinary Medical Association. 233 (1): 63–72. doi:10.2460/javma.233.1.63. ISSN 0003-1488.

[Ross1957-5] Ross, Joan M. (1957). "The pathogenesis of anthrax following the administration of spores by the respiratory route". The Journal of Pathology and Bacteriology. 73 (2): 485–494. doi:10.1002/path.1700730219. ISSN 0368-3494.

[Moayeri2004-6] Moayeri, M (2004). "The roles of anthrax toxin in pathogenesis". Current Opinion in Microbiology. 7 (1): 19–24. doi:10.1016/j.mib.2003.12.001. ISSN 1369-5274.

[7] Rubin, Raphael (2012). Rubin's pathology : clinicopathologic foundations of medicine. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins. ISBN 1605479683.

[8] Kumar, Vinay (2014). Robbins and Cotran pathologic basis of disease. Philadelphia, PA: Elsevier/Saunders. ISBN 0323266169.

[CDC-9] 9.0 ^9.1 "Anthrax Symptoms".

[10] Turnbull, Peter (2008). Anthrax in humans and animals. Geneva, Switzerland: World Health Organization. ISBN 9789241547536.

[pmid12610093-11] 11.0 ^11.1 ^11.2 ^11.3 ^11.4 ^11.5 Spencer RC (2003). "Bacillus anthracis". J Clin Pathol. 56 (3): 182–7. PMC 1769905. PMID 12610093.CS1 maint: PMC format (link)

[pmid8486963-12] 12.0 ^12.1 Friedlander AM, Welkos SL, Pitt ML, Ezzell JW, Worsham PL, Rose KJ; et al. (1993). "Postexposure prophylaxis against experimental inhalation anthrax". J Infect Dis. 167 (5): 1239–43. PMID 8486963.

[13] Turnbull, Peter (2008). Anthrax in humans and animals. Geneva, Switzerland: World Health Organization. ISBN 9789241547536.

[14] Rubin, Raphael (2012). Rubin's pathology : clinicopathologic foundations of medicine. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins. ISBN 1605479683.

[15] Kumar, Vinay (2014). Robbins and Cotran pathologic basis of disease. Philadelphia, PA: Elsevier/Saunders. ISBN 0323266169.

[GuarnerJernigan2003-16] 16.0 ^16.1 Guarner, Jeannette; Jernigan, John A.; Shieh, Wun-Ju; Tatti, Kathleen; Flannagan, Lisa M.; Stephens, David S.; Popovic, Tanja; Ashford, David A.; Perkins, Bradley A.; Zaki, Sherif R. (2003). "Pathology and Pathogenesis of Bioterrorism-Related Inhalational Anthrax". The American Journal of Pathology. 163 (2): 701–709. doi:10.1016/S0002-9440(10)63697-8. ISSN 0002-9440.

[Twenhafel2010-17] Twenhafel, N. A. (2010). "Pathology of Inhalational Anthrax Animal Models". Veterinary Pathology. 47 (5): 819–830. doi:10.1177/0300985810378112. ISSN 0300-9858.

[18] A. A. Abramova & L. M. Grinberg (1993). "[Pathology of anthrax sepsis according to materials of the infectious outbreak in 1979 in Sverdlovsk (macroscopic changes)]". Arkhiv patologii. 55 (1): 12–17. PMID 7980032. Unknown parameter |month= ignored (help)

[19] A. A. Abramova & L. M. Grinberg (1993). "[Pathology of anthrax sepsis according to materials of the infectious outbreak in 1979 in Sverdlovsk (macroscopic changes)]". Arkhiv patologii. 55 (1): 12–17. PMID 7980032. Unknown parameter |month= ignored (help)

[DixonMeselson1999-20] 20.0 ^20.1 Dixon, Terry C.; Meselson, Matthew; Guillemin, Jeanne; Hanna, Philip C. (1999). "Anthrax". New England Journal of Medicine. 341 (11): 815–826. doi:10.1056/NEJM199909093411107. ISSN 0028-4793.

[PHIL-21] 21.000 ^21.001 ^21.002 ^21.003 ^21.004 ^21.005 ^21.006 ^21.007 ^21.008 ^21.009 ^21.010 ^21.011 ^21.012 ^21.013 ^21.014 ^21.015 ^21.016 ^21.017 ^21.018 ^21.019 ^21.020 ^21.021 ^21.022 ^21.023 ^21.024 ^21.025 ^21.026 ^21.027 ^21.028 ^21.029 ^21.030 ^21.031 ^21.032 ^21.033 ^21.034 ^21.035 ^21.036 ^21.037 ^21.038 ^21.039 ^21.040 ^21.041 ^21.042 ^21.043 ^21.044 ^21.045 ^21.046 ^21.047 ^21.048 ^21.049 ^21.050 ^21.051 ^21.052 ^21.053 ^21.054 ^21.055 ^21.056 ^21.057 ^21.058 ^21.059 ^21.060 ^21.061 ^21.062 ^21.063 ^21.064 ^21.065 ^21.066 ^21.067 ^21.068 ^21.069 ^21.070 ^21.071 ^21.072 ^21.073 ^21.074 ^21.075 ^21.076 ^21.077 ^21.078 ^21.079 ^21.080 ^21.081 ^21.082 ^21.083 ^21.084 ^21.085 ^21.086 ^21.087 ^21.088 ^21.089 ^21.090 ^21.091 ^21.092 ^21.093 ^21.094 ^21.095 ^21.096 ^21.097 ^21.098 ^21.099 ^21.100 ^21.101 ^21.102 ^21.103 ^21.104 ^21.105 ^21.106 ^21.107 ^21.108 ^21.109 ^21.110 ^21.111 ^21.112 ^21.113 ^21.114 "Public Health Image Library (PHIL), Centers for Disease Control and Prevention".

[1]

[2]

[3]

[4]

[5]

[6]

[7]

[8]

[9]

[10]

[11]

[12]

[13]

[14]

[15]

[16]

[17]

[18]

[19]

[20]

[21]

@@ Line 3: / Line 3: @@
 {{CMG}}; {{AE}} {{JS}}
 ==Overview==
-The [[genetic]] material of [[Bacillus anthracis]] is coded within 1 [[chromosome]] and 2 [[plasmids]] that are fundamental for its [[toxicity]].  The [[spores]] of [[B. anthracis]] are the [[infectious]] form and can remain dormant in the environment for decades.  The bacterium causes disease through 2 mechanisms: [[toxemia]] and [[bacterial infection]].<ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>  [[B. anthracis]] begins to produce toxins within hours of germination.<ref name="HannaIreland1999">{{cite journal|last1=Hanna|first1=Philip C.|last2=Ireland|first2=John A.W.|title=Understanding Bacillus anthracis pathogenesis|journal=Trends in Microbiology|volume=7|issue=5|year=1999|pages=180–182|issn=0966842X|doi=10.1016/S0966-842X(99)01507-3}}</ref>  Protective antigen (PA) and edema factor (EF) combine to form edema toxin (ET), and PA and lethal factor (LF) combine to form lethal toxin (LT).  [[Bacterial]] [[toxins]] have a direct [[cytotoxic]] effect by interfering with cellular pathways and they are also responsible for weakening the [[immune system]] so the initial systemic [[infection]] may occur.  Anthrax lesions at any site are featured by extensive [[necrosis]] and confluent [[exudate]] containing [[macrophages]] and [[neutrophils]].
+The [[genetic]] material of [[Bacillus anthracis]] is coded within 1 [[chromosome]] and 2 [[plasmids]], which are fundamental for its [[toxicity]]. The [[spores]] of [[B. anthracis]] are the [[infectious]] form and can remain dormant in the environment for decades. The disease may be transmitted through the [[skin]], [[gastrointestinal]] or [[respiratory]] systems. The [[bacterium]] causes disease through 2 mechanisms: [[toxemia]] and [[bacterial infection]].<ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>  [[B. anthracis]] begins to produce [[toxins]] within hours of germination.<ref name="HannaIreland1999">{{cite journal|last1=Hanna|first1=Philip C.|last2=Ireland|first2=John A.W.|title=Understanding Bacillus anthracis pathogenesis|journal=Trends in Microbiology|volume=7|issue=5|year=1999|pages=180–182|issn=0966842X|doi=10.1016/S0966-842X(99)01507-3}}</ref>  Protective antigen (PA) and edema factor (EF) combine to form edema toxin (ET), and PA and lethal factor (LF) combine to form lethal toxin (LT), the active [[toxins]]. [[Bacterial]] [[toxins]] have a direct [[cytotoxic]] effect by interfering with [[cellular]] pathways, being also responsible for weakening the [[immune system]], so the initial systemic [[infection]] may occur. Anthrax lesions at any site are characterized by [[lymphadenopathy]], extensive [[edema]], [[necrosis]] and confluent [[exudate]] containing [[macrophages]] and [[neutrophils]]. If not stopped, the [[infection]] may affect different [[organs]], causing [[septicemia]] and potentially death.
 ==Genetics==
-[[Bacillus anthracis]] [[genetic]] component includes 1 [[chromosome]] and 2 [[plasmids]]. These [[plasmids]] (pXO1 and pXO2) are fundamental to its [[toxicity]]:<ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>
+The [[genetic]] component of [[Bacillus anthracis]] includes 1 [[chromosome]] and 2 [[plasmids]]. These [[plasmids]] (pXO1 and pXO2) are fundamental for its [[toxicity]]:<ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>
-* pXO1 - encodes 3 components of the anthrax [[exotoxin]]:
+* pXO1 - encodes 3 components of the anthrax [[exotoxin]]s:
 :* Protective Antigen (PA)
 :* Lethal Factor (LF)
 :* Edema Factor (EF)
-* pXO2 - encodes proteins involved in the [[glutamic acid]] [[capsule]] structure, which is responsible for conferring [[resistance]] against [[phagocytosis]].
+* pXO2 - encodes proteins involved in the [[glutamic acid]] [[capsule]] structure which is responsible for conferring [[resistance]] against [[phagocytosis]].
 ==Transmission==
-The route by which anthrax is transmitted allows for its classification, it includes:<ref name=WHO>{{cite web | title = Anthrax in Humans and Animals | url = http://www.who.int/csr/resources/publications/anthrax_web.pdf }}</ref>
+The route of transmission of anthrax allows for its classification into the following:<ref name=WHO>{{cite web | title = Anthrax in Humans and Animals | url = http://www.who.int/csr/resources/publications/anthrax_web.pdf }}</ref>
 * Cutaneous anthrax - commonly requires a prior [[skin lesion]] as a prerequisite for [[infection]]
-* Gastrointestinal anthrax - contracted following ingestion of contaminated food, primarily meat from an animal that died of the disease, or conceivably from ingestion of contaminated water
+* Gastrointestinal anthrax - contracted following [[ingestion]] of contaminated food, primarily meat from an animal that died of the disease, or conceivably from [[ingestion]] of contaminated water
 * Inhalational anthrax - from breathing in airborne anthrax [[spores]]
-* Injection anthrax - from injection of a drug containing, or contaminated with [[Bacillus anthracis]]
+* Injection anthrax - from the injection of a [[drug]] containing or contaminated with [[Bacillus anthracis]]
 ==Pathogenesis==
 [[B. anthracis]], the causative agent of anthrax, is a [[spore]]-forming [[bacterium]]. The [[spores]] of [[B. anthracis]], which can remain dormant in the environment for decades, are the [[infectious]] form, but this vegetative form of [[B. anthracis]] rarely causes disease.<ref name="ShadomySmith2008">{{cite journal|last1=Shadomy|first1=Sean V.|last2=Smith|first2=Theresa L.|title=Anthrax|journal=Journal of the American Veterinary Medical Association|volume=233|issue=1|year=2008|pages=63–72|issn=0003-1488|doi=10.2460/javma.233.1.63}}</ref> The [[bacterium]] causes disease through 2 mechanisms: [[toxemia]] and [[bacterial infection]].<ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>
-[[Spores]] introduced through the [[skin]] lead to [[cutaneous]] or injection anthrax; those introduced through the [[gastrointestinal]] tract lead to gastrointestinal anthrax; and those introduced through the [[lungs]] lead to inhalation anthrax. After entering a human or animal, [[B. anthracis]] [[spores]] are believed to germinate locally or be phagocytosed by [[dendritic cells]] and [[macrophages]]. These will then carry the [[spores]] to the [[lymph nodes]], where they germinate.<ref name="Ross1957">{{cite journal|last1=Ross|first1=Joan M.|title=The pathogenesis of anthrax following the administration of spores by the respiratory route|journal=The Journal of Pathology and Bacteriology|volume=73|issue=2|year=1957|pages=485–494|issn=0368-3494|doi=10.1002/path.1700730219}}</ref><ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>
+[[Spores]] introduced through the [[skin]] lead to [[cutaneous]] or injection anthrax; those introduced through the [[gastrointestinal]] tract lead to gastrointestinal anthrax; and those introduced through the [[lungs]] lead to inhalation anthrax. After entering a human or animal, [[B. anthracis]] [[spores]] are believed to germinate locally or be [[phagocytosed]] by [[dendritic cells]] and [[macrophages]]. These will then carry the [[spores]] to the [[lymph nodes]], where they germinate.<ref name="Ross1957">{{cite journal|last1=Ross|first1=Joan M.|title=The pathogenesis of anthrax following the administration of spores by the respiratory route|journal=The Journal of Pathology and Bacteriology|volume=73|issue=2|year=1957|pages=485–494|issn=0368-3494|doi=10.1002/path.1700730219}}</ref><ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>
-[[B. anthracis]] begins to produce toxins within hours of germination.<ref name="HannaIreland1999">{{cite journal|last1=Hanna|first1=Philip C.|last2=Ireland|first2=John A.W.|title=Understanding Bacillus anthracis pathogenesis|journal=Trends in Microbiology|volume=7|issue=5|year=1999|pages=180–182|issn=0966842X|doi=10.1016/S0966-842X(99)01507-3}}</ref>
+[[B. anthracis]] begins to produce [[toxins]] within hours of germination.<ref name="HannaIreland1999">{{cite journal|last1=Hanna|first1=Philip C.|last2=Ireland|first2=John A.W.|title=Understanding Bacillus anthracis pathogenesis|journal=Trends in Microbiology|volume=7|issue=5|year=1999|pages=180–182|issn=0966842X|doi=10.1016/S0966-842X(99)01507-3}}</ref>
-Protective antigen (PA) and edema factor (EF) combine to form edema toxin (ET), and PA and lethal factor (LF) combine to form lethal toxin (LT). After binding to surface receptors, the PA portion of the complexes facilitates translocation of the toxins to the [[cytosol]], in which EF and LF exert their toxic effects.<ref name="Moayeri2004">{{cite journal|last1=Moayeri|first1=M|title=The roles of anthrax toxin in pathogenesis|journal=Current Opinion in Microbiology|volume=7|issue=1|year=2004|pages=19–24|issn=13695274|doi=10.1016/j.mib.2003.12.001}}</ref>
+Protective antigen (PA) and edema factor (EF) combine to form edema toxin (ET), and PA and lethal factor (LF) combine to form lethal toxin (LT). After binding to surface receptors, the PA portion of the complexes facilitates translocation of the [[toxins]] to the [[cytosol]], in which EF and LF exert their toxic effects.<ref name="Moayeri2004">{{cite journal|last1=Moayeri|first1=M|title=The roles of anthrax toxin in pathogenesis|journal=Current Opinion in Microbiology|volume=7|issue=1|year=2004|pages=19–24|issn=13695274|doi=10.1016/j.mib.2003.12.001}}</ref>
-Bacillus anthracis disseminate to multiple organs including [[spleen]], [[liver]], [[intestines]], [[kidneys]], [[adrenal glands]], and [[meninges]], affecting their functioning, leading to systemic infection and a potentially fatal outcome.<ref>{{cite book | last = Rubin | first = Raphael | title = Rubin's pathology : clinicopathologic foundations of medicine | publisher = Wolters Kluwer Health/Lippincott Williams & Wilkins | location = Philadelphia | year = 2012 | isbn = 1605479683 }}</ref><ref>{{cite book | last = Kumar | first = Vinay | title = Robbins and Cotran pathologic basis of disease | publisher = Elsevier/Saunders | location = Philadelphia, PA | year = 2014 | isbn = 0323266169 }}</ref><ref name=WHO>{{cite web | title = Anthrax in Humans and Animals | url = http://www.who.int/csr/resources/publications/anthrax_web.pdf }}</ref>
+[[Bacillus anthracis]] disseminate to multiple organs including [[spleen]], [[liver]], [[intestines]], [[kidneys]], [[adrenal glands]], and [[meninges]], affecting their normal functions and leading to systemic [[infection]] with a potentially fatal outcome.<ref>{{cite book | last = Rubin | first = Raphael | title = Rubin's pathology : clinicopathologic foundations of medicine | publisher = Wolters Kluwer Health/Lippincott Williams & Wilkins | location = Philadelphia | year = 2012 | isbn = 1605479683 }}</ref><ref>{{cite book | last = Kumar | first = Vinay | title = Robbins and Cotran pathologic basis of disease | publisher = Elsevier/Saunders | location = Philadelphia, PA | year = 2014 | isbn = 0323266169 }}</ref><ref name=WHO>{{cite web | title = Anthrax in Humans and Animals | url = http://www.who.int/csr/resources/publications/anthrax_web.pdf }}</ref>
 The [[virulence factors]] of [[Bacillus anthracis]] are:
@@ Line 37: / Line 37: @@
 ===Bacterial Toxins===
-In order to infect the body, [[Bacillus anthracis]] must produce [[toxins]]. Toxins have 3 main toxic effects: [[edema]], [[hemorrhage]] and [[necrosis]]. Besides their direct [[toxic]] effects, causing tissue damage, [[anthrax]] [[toxins]] are also responsible for interfering with [[cellular]] pathways in such way, that defense functions of the host's [[immune system]] are affected. This will ultimately allow initial systemic [[infection]], by interfering with the [[immune system]].<ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>
+In order to [[infect]] the body, [[Bacillus anthracis]] must produce [[toxins]]. These toxins have 3 main toxic effects: [[edema]], [[hemorrhage]], and [[necrosis]]. Besides their direct [[toxic]] effects responsible for tissue damage, [[anthrax]] [[toxins]] are also responsible for interfering with [[cellular]] pathways, in such way that defense functions of the host's [[immune system]] are affected. This will ultimately allow initial systemic [[infection]] by interfering with the [[immune system]].<ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>
 When isolated, the 3 structural elements of the anthrax [[exotoxins]] are non-toxic. However, when combined, they form [[virulent]] [[exotoxins]]:<ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>
@@ Line 46: / Line 46: @@
 The PA is responsible for attaching the [[toxin]] to the [[cell]], while the LF and the EF are responsible for the [[toxicity]].<ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>
-After germinating, [[B. anthracis]] produces and releases into the [[blood stream]] PA, LF and EF [[toxins]] separately. However, PA is secreted in its inactivated form (PA). In order to form [[exotoxin]] complexes with LF and EF, it must first be activated by host-cellular receptors:<ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>
+After germinating, [[B. anthracis]] produces and releases into the [[blood stream]] PA, LF, and EF [[toxins]] separately. However, PA is secreted in its inactivated form (PA). In order to form the [[exotoxin]] complexes with LF and EF, it must first be activated by host-cellular receptors:<ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>
-* CMG2 - ''Capillary Morphogenesis Protein 2'' (''in vivo'' predominant toxin receptor)
+* CMG2 - ''Capillary Morphogenesis Protein 2'' (predominant toxin receptor''in vivo'')
 * TEM8 - ''Tumor Endothelium Marker 8'' (minor role)
@@ Line 54: / Line 54: @@
 The acidic environment within the [[endosomes]] leads to the formation of a channel called ''PA63 oligomer channel'', on the endosomal membrane. LF and EF are then released in the [[cytosol]] of the host [[cell]], to then exert their [[toxic]] effects.<ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>
-After experiments in mice, edema toxin was noted to be the major [[virulence factor]] since it caused death of mice, in much lesser dosages than lethal toxin. edema toxin is a calmodulin-dependent [[adenylyl cyclase]]  known to increase [[intracellular]] [[cAMP]], through the conversion of [[ATP]] into [[cAMP]], thus affecting several [[intracellular]] pathways. Lethal toxin is a zinc-dependent metaloproteinase, known to interfere with the mitogen-activated protein kinase (MEK), thereby hampering with multiple [[intracellular]] mechanisms.<ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>
+After experiments in mice, edema toxin was noted to be the major [[virulence factor]] since it caused death of mice in much lesser dosages than lethal toxin.
+* Edema toxin is a calmodulin-dependent [[adenylyl cyclase]], known to increase [[intracellular]] [[cAMP]] through the conversion of [[ATP]] into [[cAMP]], thus affecting several [[intracellular]] pathways.
+* Lethal toxin is a zinc-dependent metaloproteinase known to interfere with the mitogen-activated protein kinase (MEK), thereby hampering multiple [[intracellular]] mechanisms.<ref name="LiuMoayeri2014">{{cite journal|last1=Liu|first1=Shihui|last2=Moayeri|first2=Mahtab|last3=Leppla|first3=Stephen H.|title=Anthrax lethal and edema toxins in anthrax pathogenesis|journal=Trends in Microbiology|volume=22|issue=6|year=2014|pages=317–325|issn=0966842X|doi=10.1016/j.tim.2014.02.012}}</ref>
 ===Cutaneous or Injection Anthrax===
-According to animal studies, injected [[spores]] in the [[skin]] of susceptible animals (either through a lesion or by injection), germinated and gave rise, in about 2 - 4 hours, to a small [[edema|edematous]] area containing capsulated [[bacilli]]. The following stages were noticed:<ref name=WHO>{{cite web | title = Anthrax in Humans and Animals | url = http://www.who.int/csr/resources/publications/anthrax_web.pdf }}</ref>
+According to animal studies, [[spores]] that enter the [[skin]] of susceptible animals (either through a lesion or by injection) germinate and give rise, in about 2 - 4 hours, to a small [[edema|edematous]] area containing capsulated [[bacilli]]. The following stages are noticed:<ref name=WHO>{{cite web | title = Anthrax in Humans and Animals | url = http://www.who.int/csr/resources/publications/anthrax_web.pdf }}</ref>
-* The lesion increases in size and becomes defined by a zone of altered [[capillaries]] through [[dilatation]], [[congestion]] and [[swelling]] of the [[endothelial cells]], and [[diapedesis]] of [[neutrophils]]
+* The lesion increases in size and becomes defined by a zone of altered [[capillaries]] through [[dilatation]], [[congestion]], and [[swelling]] of the [[endothelial cells]] and through [[diapedesis]] of [[neutrophils]].
-* [[Macrophages]] and [[fibrin]] deposits appear, and the efferent [[lymphatics]] become dilated
+* [[Macrophages]] and [[fibrin]] deposits appear, and the efferent [[lymphatics]] become dilated.
-* Continued multiplication of the [[bacilli]] in the lesion leads to fragmentation of the [[connective tissue]], as the [[edema]] spreads extensively away from the [[infection]] site
+* Continued multiplication of the [[bacilli]] in the lesion leads to fragmentation of the [[connective tissue]], as the [[edema]] spreads extensively away from the [[infection]] site.
-* [[Necrosis]] and [[hemorrhage]] occur. If initial [[inoculation]] has happened with minimal number of [[spores]], the primary lesion may be insignificant compared with that of the draining [[lymph node]]s
+* [[Necrosis]] and [[hemorrhage]] occur. If initial [[inoculation]] has happened with minimal number of [[spores]], the primary lesion may be insignificant compared with that of the draining [[lymph node]]s.
-* The [[bacilli]] migrate from the primary lesion, by means of the [[lymph vessel]]s, to the local [[lymph nodes]] where multiplication occurs, releasing a continuous stream of organisms and [[toxins]] into the efferent [[lymph vessel]]s and to the [[spleen]] and other lymphoid tissues where multiplication continues
+* The [[bacilli]] migrate from the primary lesion, by means of the [[lymph vessel]]s, to the local [[lymph nodes]] where multiplication occurs, releasing a continuous stream of organisms and [[toxins]] into the efferent [[lymph vessel]]s and to the [[spleen]] and other lymphoid tissues where multiplication continues.
-* In animals who died following [[subcutaneous]] challenge, the local [[lymph node]]s became [[hemorrhage|haemorrhagic]] and [[edema|edematous]]. [[Necrosis]] of lymphatic elements and of [[blood vessel]] walls occurs and [[phagocytosis]] of the [[bacilli]] was apparent there
+* In animals who died following [[subcutaneous]] challenge, the local [[lymph node]]s became [[hemorrhage|haemorrhagic]] and [[edema|edematous]]. [[Necrosis]] of lymphatic elements and of [[blood vessel]] walls occur and [[phagocytosis]] of the [[bacilli]] is apparent.
-* [[Splenic]] enlargement is characteristic of [[septicemia]]
+*  [[Septicemia]] often courses with [[splenic]] enlargement
-Injection anthrax will have similar [[pathogenesis]] to cutaneous anthrax, but since it is injected, it can spread throughout the body faster and be harder to recognize and treat than the cutaneous form.<ref name=CDC>{{cite web | title = Anthrax Symptoms | url = http://www.cdc.gov/anthrax/basics/symptoms.html }}</ref>
+Injection anthrax will have similar [[pathogenesis]] to cutaneous anthrax, but since it is injected, it can spread throughout the body faster and it becomes harder to recognize and treat than the [[cutaneous]] form.<ref name=CDC>{{cite web | title = Anthrax Symptoms | url = http://www.cdc.gov/anthrax/basics/symptoms.html }}</ref>
 ===Inhalation Anthrax===
-In inhalation anthrax, the inhaled spores will be deposited in the alveoli first. From there, they will be transported, within [[phagocytic cells]], through the [[lymphatic vessel]]s to the [[mediastinal]] [[lymph node]]s, where they will grow and cause [[hemorrhage|hemorrhagic]] [[lymphadenitis]]. [[Bacillus anthracis|Bacteria]] escape from the damaged [[lymph node]]s and invade the [[blood stream]] via the [[thoracic duct]]. Vegetative [[Bacillus]] then travel through the [[bloodstream]] and [[lymph vessel]]s, potentially causing [[septicemia]]. At the same time [[toxins]] are released, causing tissue damage and hampering the [[immune system]], in order to facilitate [[bacterial]] spread. Once the [[bacteremia]] and associated [[toxemia]] reach a critical level, the severe [[symptoms]] characteristic of the acute phase of illness are manifested. During the acute phase, damage of the [[lung]] tissue becomes apparent on the [[X-ray]]. This damage results from the action of [[anthrax toxin]] on the [[endothelium]] of the lung’s [[capillary bed]]. Primary damage of the [[lung]] is not normally a feature of the initial phase of illness and primary [[pulmonary]] [[infection]] is an uncommon presentation.<ref>{{cite book | last = Turnbull | first = Peter | title = Anthrax in humans and animals | publisher = World Health Organization | location = Geneva, Switzerland | year = 2008 | isbn = 9789241547536 }}</ref><ref name="pmid12610093">{{cite journal| author=Spencer RC| title=Bacillus anthracis. | journal=J Clin Pathol | year= 2003 | volume= 56 | issue= 3 | pages= 182-7 | pmid=12610093 | doi= | pmc=PMC1769905 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=12610093  }} </ref><ref name="pmid8486963">{{cite journal| author=Friedlander AM, Welkos SL, Pitt ML, Ezzell JW, Worsham PL, Rose KJ et al.| title=Postexposure prophylaxis against experimental inhalation anthrax. | journal=J Infect Dis | year= 1993 | volume= 167 | issue= 5 | pages= 1239-43 | pmid=8486963 | doi= | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=8486963  }} </ref>
+In inhalation anthrax, the inhaled [[spores]] will be deposited in the [[alveoli]] first. From there, they will be transported, within [[phagocytic cells]], through the [[lymphatic vessel]]s to the [[mediastinal]] [[lymph node]]s, where they will grow and cause [[hemorrhage|hemorrhagic]] [[lymphadenitis]]. [[Bacillus anthracis|Bacteria]] escape from the damaged [[lymph node]]s and invade the [[blood stream]] via the [[thoracic duct]]. Vegetative [[Bacillus]] then travel through the [[bloodstream]] and [[lymph vessel]]s, potentially causing [[septicemia]]. At the same time [[toxins]] are released, causing tissue damage and hampering the [[immune system]] to facilitate [[bacterial]] spread.<ref>{{cite book | last = Turnbull | first = Peter | title = Anthrax in humans and animals | publisher = World Health Organization | location = Geneva, Switzerland | year = 2008 | isbn = 9789241547536 }}</ref><ref name="pmid12610093">{{cite journal| author=Spencer RC| title=Bacillus anthracis. | journal=J Clin Pathol | year= 2003 | volume= 56 | issue= 3 | pages= 182-7 | pmid=12610093 | doi= | pmc=PMC1769905 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=12610093  }} </ref><ref name="pmid8486963">{{cite journal| author=Friedlander AM, Welkos SL, Pitt ML, Ezzell JW, Worsham PL, Rose KJ et al.| title=Postexposure prophylaxis against experimental inhalation anthrax. | journal=J Infect Dis | year= 1993 | volume= 167 | issue= 5 | pages= 1239-43 | pmid=8486963 | doi= | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=8486963  }} </ref>
-Studies in rhesus monkeys revealed that after spore inhalation, its germination might take up to 60 days. These is reason for the recommended antibiotic prophylaxis for 60 days.<ref name="pmid12610093">{{cite journal| author=Spencer RC| title=Bacillus anthracis. | journal=J Clin Pathol | year= 2003 | volume= 56 | issue= 3 | pages= 182-7 | pmid=12610093 | doi= | pmc=PMC1769905 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=12610093  }} </ref>
+Once the [[bacteremia]] and associated [[toxemia]] reach a critical level, the severe [[symptoms]] that are characteristic of the acute phase of illness are manifested. During the acute phase, damage of the [[lung]] tissue becomes apparent on the [[X-ray]]. This damage results from the action of [[anthrax toxin]] on the [[endothelium]] of the lung’s [[capillary bed]]. Primary damage of the [[lung]] is not normally a feature of the initial phase of illness and primary [[pulmonary]] [[infection]] is an uncommon presentation.<ref>{{cite book | last = Turnbull | first = Peter | title = Anthrax in humans and animals | publisher = World Health Organization | location = Geneva, Switzerland | year = 2008 | isbn = 9789241547536 }}</ref><ref name="pmid12610093">{{cite journal| author=Spencer RC| title=Bacillus anthracis. | journal=J Clin Pathol | year= 2003 | volume= 56 | issue= 3 | pages= 182-7 | pmid=12610093 | doi= | pmc=PMC1769905 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=12610093  }} </ref><ref name="pmid8486963">{{cite journal| author=Friedlander AM, Welkos SL, Pitt ML, Ezzell JW, Worsham PL, Rose KJ et al.| title=Postexposure prophylaxis against experimental inhalation anthrax. | journal=J Infect Dis | year= 1993 | volume= 167 | issue= 5 | pages= 1239-43 | pmid=8486963 | doi= | pmc= | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=8486963  }} </ref>
+Studies in rhesus monkeys revealed that after [[spore]] [[inhalation]], its germination might take up to 60 days. This is the reason why [[antibiotic]] [[prophylaxis]] is recommended for 60 days.<ref name="pmid12610093">{{cite journal| author=Spencer RC| title=Bacillus anthracis. | journal=J Clin Pathol | year= 2003 | volume= 56 | issue= 3 | pages= 182-7 | pmid=12610093 | doi= | pmc=PMC1769905 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=12610093  }} </ref>
 ===Gastrointestinal Anthrax===
-In animal studies the [[intestinal]] lesions are focal to diffuse [[hemorrhage|hemorrhagic]] [[necrotic]] [[enteritis]] of the [[small intestine]]. The tendency for localized lesions to develop in [[Peyer's patches]] suggests a possible role of the [[M cell]] in the uptake of the anthrax [[bacillus]].<ref name=WHO>{{cite web | title = Anthrax in Humans and Animals | url = http://www.who.int/csr/resources/publications/anthrax_web.pdf }}</ref>
+In animal studies, the [[intestinal]] lesions caused by ingested anthrax [[spores]] range from focal to diffuse [[hemorrhage|hemorrhagic]] [[necrotic]] [[enteritis]] of the [[small intestine]]. The tendency for localized lesions to develop in [[Peyer's patches]] suggests a possible role of the [[M cell]] in the uptake of the anthrax [[bacillus]].<ref name=WHO>{{cite web | title = Anthrax in Humans and Animals | url = http://www.who.int/csr/resources/publications/anthrax_web.pdf }}</ref>
 ==Gross Pathology==
 ===Cutaneous and Injection Anthrax===
-[[Cutaneous]] [[infection]] typically produces ulcerated lesions which are covered by a back scab and often contain numerous [[microorganisms]]. Anthrax eschars are generally seen on exposed unprotected regions of the body, mostly on the face, neck, hands and wrists. Generally [[cutaneous]] lesions are single, but sometimes two or more lesions are present.<ref>{{cite book | last = Rubin | first = Raphael | title = Rubin's pathology : clinicopathologic foundations of medicine | publisher = Wolters Kluwer Health/Lippincott Williams & Wilkins | location = Philadelphia | year = 2012 | isbn = 1605479683 }}</ref><ref>{{cite book | last = Kumar | first = Vinay | title = Robbins and Cotran pathologic basis of disease | publisher = Elsevier/Saunders | location = Philadelphia, PA | year = 2014 | isbn = 0323266169 }}</ref>
+[[Cutaneous]] [[infection]] typically produces [[ulcer|ulcerated]] lesions which are covered by a scab and often contain numerous [[microorganisms]]. Anthrax [[eschar]]s are generally seen on exposed unprotected regions of the body, mostly on the [[face]], [[neck]], [[hands]] and [[wrist]]s. Generally [[cutaneous]] lesions are single, but sometimes two or more lesions are present.<ref>{{cite book | last = Rubin | first = Raphael | title = Rubin's pathology : clinicopathologic foundations of medicine | publisher = Wolters Kluwer Health/Lippincott Williams & Wilkins | location = Philadelphia | year = 2012 | isbn = 1605479683 }}</ref><ref>{{cite book | last = Kumar | first = Vinay | title = Robbins and Cotran pathologic basis of disease | publisher = Elsevier/Saunders | location = Philadelphia, PA | year = 2014 | isbn = 0323266169 }}</ref>
-The lesions produced by injection contrast will be similar to the ones of the cutaneous form. The difference will reside on the fact that injection anthrax can spread throughout the body faster and be harder to recognize and treat than cutaneous anthrax.<ref name=CDC>{{cite web | title = Anthrax Symptoms | url = http://www.cdc.gov/anthrax/basics/symptoms.html }}</ref>
+The lesions produced by injection anthrax will be similar to the ones of the [[cutaneous]] form. The difference will reside on the fact that injection anthrax can spread throughout the body faster and be harder to recognize and treat than cutaneous anthrax.<ref name=CDC>{{cite web | title = Anthrax Symptoms | url = http://www.cdc.gov/anthrax/basics/symptoms.html }}</ref>
 ===Inhalational Anthrax===
-Gross pathologic lesions observed in non-human primates used in aerosol challenge models of inhalation anthrax include [[edema]], [[congestion]], [[hemorrhage]]  and [[necrosis]] in the [[lungs]] and [[mediastinum]]. [[Splenitis]] and necrotizing or hemorrhagic [[lymphadenitis]] involving the mediastinal, tracheobronchial, and other [[lymph nodes]] are common.<ref name="GuarnerJernigan2003">{{cite journal|last1=Guarner|first1=Jeannette|last2=Jernigan|first2=John A.|last3=Shieh|first3=Wun-Ju|last4=Tatti|first4=Kathleen|last5=Flannagan|first5=Lisa M.|last6=Stephens|first6=David S.|last7=Popovic|first7=Tanja|last8=Ashford|first8=David A.|last9=Perkins|first9=Bradley A.|last10=Zaki|first10=Sherif R.|title=Pathology and Pathogenesis of Bioterrorism-Related Inhalational Anthrax|journal=The American Journal of Pathology|volume=163|issue=2|year=2003|pages=701–709|issn=00029440|doi=10.1016/S0002-9440(10)63697-8}}</ref> Primary [[pulmonary]] lesions, including those of [[pneumonia]], are occasionally observed. [[Meningeal]] involvement ranging from [[edema]], [[congestion]], [[hemorrhage]], and [[necrosis]] to [[suppurative]] or hemorrhagic [[meningitis]], usually secondary to hematogenous spread from other types of [[anthrax]], occurs in ≤77% of animals studied.<ref name="Twenhafel2010">{{cite journal|last1=Twenhafel|first1=N. A.|title=Pathology of Inhalational Anthrax Animal Models|journal=Veterinary Pathology|volume=47|issue=5|year=2010|pages=819–830|issn=0300-9858|doi=10.1177/0300985810378112}}</ref> Autopsy findings for persons who died from inhalation anthrax in Sverdlovsk and in the United States<ref>{{Cite journal| author = [[A. A. Abramova]] & [[L. M. Grinberg]] | title = &#91;Pathology of anthrax sepsis according to materials of the infectious outbreak in 1979 in Sverdlovsk (macroscopic changes)&#93; | journal = [[Arkhiv patologii]] | volume = 55 | issue = 1 | pages = 12–17 | year = 1993 | month = January-February | pmid = 7980032}}</ref> are consistent with findings from the non-human primates studies. Persons who died had extensive amounts of serosanguinous fluid in [[pleural]] cavities and [[edema]] and [[hemorrhage]] of the [[mediastinum]] and surrounding soft tissues, and 48% had cerebral [[edema]], 21% had [[ascites]], 17% had [[pericardial effusion]]s, and 14% had [[petechial rash]]. [[Mediastinal]] [[lymph nodes]] and [[spleen]] also showed [[hemorrhage]] and [[necrosis]].<ref name="GuarnerJernigan2003">{{cite journal|last1=Guarner|first1=Jeannette|last2=Jernigan|first2=John A.|last3=Shieh|first3=Wun-Ju|last4=Tatti|first4=Kathleen|last5=Flannagan|first5=Lisa M.|last6=Stephens|first6=David S.|last7=Popovic|first7=Tanja|last8=Ashford|first8=David A.|last9=Perkins|first9=Bradley A.|last10=Zaki|first10=Sherif R.|title=Pathology and Pathogenesis of Bioterrorism-Related Inhalational Anthrax|journal=The American Journal of Pathology|volume=163|issue=2|year=2003|pages=701–709|issn=00029440|doi=10.1016/S0002-9440(10)63697-8}}</ref><ref>{{Cite journal| author = [[A. A. Abramova]] & [[L. M. Grinberg]] | title = &#91;Pathology of anthrax sepsis according to materials of the infectious outbreak in 1979 in Sverdlovsk (macroscopic changes)&#93; | journal = [[Arkhiv patologii]] | volume = 55 | issue = 1 | pages = 12–17 | year = 1993 | month = January-February | pmid = 7980032}}</ref>
+Gross pathologic lesions observed in non-human primates used in aerosol challenge models of inhalation anthrax include [[edema]], [[congestion]], [[hemorrhage]], and [[necrosis]] in the [[lungs]] and [[mediastinum]]. [[Splenitis]] and [[necrotizing]] or [[hemorrhage|hemorrhagic]] [[lymphadenitis]] involving the [[mediastinal]], [[tracheobronchial]], and other [[lymph nodes]] are common.<ref name="GuarnerJernigan2003">{{cite journal|last1=Guarner|first1=Jeannette|last2=Jernigan|first2=John A.|last3=Shieh|first3=Wun-Ju|last4=Tatti|first4=Kathleen|last5=Flannagan|first5=Lisa M.|last6=Stephens|first6=David S.|last7=Popovic|first7=Tanja|last8=Ashford|first8=David A.|last9=Perkins|first9=Bradley A.|last10=Zaki|first10=Sherif R.|title=Pathology and Pathogenesis of Bioterrorism-Related Inhalational Anthrax|journal=The American Journal of Pathology|volume=163|issue=2|year=2003|pages=701–709|issn=00029440|doi=10.1016/S0002-9440(10)63697-8}}</ref> Primary [[pulmonary]] lesions, including those of [[pneumonia]], are occasionally observed. [[Meningeal]] involvement ranging from [[edema]], [[congestion]], [[hemorrhage]], and [[necrosis]] to [[suppurative]] or [[hemorrhage|hemorrhagic]] [[meningitis]], usually secondary to hematogenous spread from other types of [[anthrax]], occurs in ≤77% of animals studied.<ref name="Twenhafel2010">{{cite journal|last1=Twenhafel|first1=N. A.|title=Pathology of Inhalational Anthrax Animal Models|journal=Veterinary Pathology|volume=47|issue=5|year=2010|pages=819–830|issn=0300-9858|doi=10.1177/0300985810378112}}</ref> Autopsy findings from persons who died from inhalation anthrax in Sverdlovsk and in the United States<ref>{{Cite journal| author = [[A. A. Abramova]] & [[L. M. Grinberg]] | title = &#91;Pathology of anthrax sepsis according to materials of the infectious outbreak in 1979 in Sverdlovsk (macroscopic changes)&#93; | journal = [[Arkhiv patologii]] | volume = 55 | issue = 1 | pages = 12–17 | year = 1993 | month = January-February | pmid = 7980032}}</ref> are consistent with findings from the non-human primates studies. Persons who died had extensive amounts of serosanguinous fluid in [[pleural]] cavities, [[edema]], and [[hemorrhage]] of the [[mediastinum]] and surrounding soft tissues. 48% had cerebral [[edema]], 21% had [[ascites]], 17% had [[pericardial effusion]]s, and 14% had [[petechial rash]]. [[Mediastinal]] [[lymph nodes]] and [[spleen]] also showed [[hemorrhage]] and [[necrosis]].<ref name="GuarnerJernigan2003">{{cite journal|last1=Guarner|first1=Jeannette|last2=Jernigan|first2=John A.|last3=Shieh|first3=Wun-Ju|last4=Tatti|first4=Kathleen|last5=Flannagan|first5=Lisa M.|last6=Stephens|first6=David S.|last7=Popovic|first7=Tanja|last8=Ashford|first8=David A.|last9=Perkins|first9=Bradley A.|last10=Zaki|first10=Sherif R.|title=Pathology and Pathogenesis of Bioterrorism-Related Inhalational Anthrax|journal=The American Journal of Pathology|volume=163|issue=2|year=2003|pages=701–709|issn=00029440|doi=10.1016/S0002-9440(10)63697-8}}</ref><ref>{{Cite journal| author = [[A. A. Abramova]] & [[L. M. Grinberg]] | title = &#91;Pathology of anthrax sepsis according to materials of the infectious outbreak in 1979 in Sverdlovsk (macroscopic changes)&#93; | journal = [[Arkhiv patologii]] | volume = 55 | issue = 1 | pages = 12–17 | year = 1993 | month = January-February | pmid = 7980032}}</ref>
 ===Gastrointestinal Anthrax===
-On [[gastrointestinal]] [[infection]] the typical [[eschar]] may occur on different locations:<ref name="pmid12610093">{{cite journal| author=Spencer RC| title=Bacillus anthracis. | journal=J Clin Pathol | year= 2003 | volume= 56 | issue= 3 | pages= 182-7 | pmid=12610093 | doi= | pmc=PMC1769905 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=12610093  }} </ref>
+On [[gastrointestinal]] [[infection]] the typical [[eschar]] may occur on different locations, including:<ref name="pmid12610093">{{cite journal| author=Spencer RC| title=Bacillus anthracis. | journal=J Clin Pathol | year= 2003 | volume= 56 | issue= 3 | pages= 182-7 | pmid=12610093 | doi= | pmc=PMC1769905 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=12610093  }} </ref>
-* Oropharynx
+* [[Oropharynx]]
-* Stomach
+* [[Stomach]]
-* Duodenum
+* [[Duodenum]]
-* Ileum
+* [[Ileum]]
-* Caecum
+* [[Cecum]]
 According to the location of the [[eschar]], gastrointestinal anthrax may be divided in 2 categories: [[oropharyngeal]] and [[abdominal]].<ref name="pmid12610093">{{cite journal| author=Spencer RC| title=Bacillus anthracis. | journal=J Clin Pathol | year= 2003 | volume= 56 | issue= 3 | pages= 182-7 | pmid=12610093 | doi= | pmc=PMC1769905 | url=http://www.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&tool=sumsearch.org/cite&retmode=ref&cmd=prlinks&id=12610093  }} </ref>
@@ Line 98: / Line 101: @@
 ==Microscopic Pathology==
-Anthrax lesions at any site are characterized by extensive [[necrosis]] and confluent [[exudate]], containing [[macrophages]] and [[neutrophils]]. In [[histopathological]] specimens or culture media, the presence of large boxcar-shaped [[Gram-positive]] [[bacilli]] in chains, suggests the [[diagnosis]].
+Anthrax lesions at any site are characterized by extensive [[necrosis]] and confluent [[exudate]], containing [[macrophages]] and [[neutrophils]]. In [[histopathological]] specimens or culture media, the presence of large boxcar-shaped [[Gram-positive]] [[bacilli]] in chains suggests the [[diagnosis]].
 ===Cutaneous or Injection Anthrax===
-[[Histologic]] examination of [[skin lesions]] caused by cutaneous anthrax revealed:<ref name="DixonMeselson1999">{{cite journal|last1=Dixon|first1=Terry C.|last2=Meselson|first2=Matthew|last3=Guillemin|first3=Jeanne|last4=Hanna|first4=Philip C.|title=Anthrax|journal=New England Journal of Medicine|volume=341|issue=11|year=1999|pages=815–826|issn=0028-4793|doi=10.1056/NEJM199909093411107}}</ref>
+[[Histologic]] examination of [[skin lesions]] caused by cutaneous anthrax reveals:<ref name="DixonMeselson1999">{{cite journal|last1=Dixon|first1=Terry C.|last2=Meselson|first2=Matthew|last3=Guillemin|first3=Jeanne|last4=Hanna|first4=Philip C.|title=Anthrax|journal=New England Journal of Medicine|volume=341|issue=11|year=1999|pages=815–826|issn=0028-4793|doi=10.1056/NEJM199909093411107}}</ref>
 * Tissue [[necrosis]]
 * [[Edema]]
@@ Line 112: / Line 115: @@
 ===Inhalation Anthrax===
-[[Histologic]] evaluation of affected tissues revealed:
+Histologic evaluation of affected tissues reveals:
-* Numerous [[necrotic]] foci of [[hemorrhage]] in the [[mediastinum]] or hilar and peribronchial [[lymph nodes]]
+* Numerous [[necrotic]] foci of [[hemorrhage]] in the [[mediastinum]] or [[hilar]] and peribronchial [[lymph nodes]]
 * [[Pulmonary]] [[infection]] may also result in interstitial [[pneumonia]] associated with:
 :* [[Vasculitis]]
@@ Line 128: / Line 131: @@
 * Occasionally [[mediastinal]] widening
-==Image Gallery==
+==Gallery==
 <gallery>
 Image:CutaneousA6.jpg| Low-power magnification of 10X of a digital Keyence scope, this photograph depicts the colonial growth displayed by Sterne strain members of the Gram-positive bacterium, Bacillus anthracis, which were cultured on sheep blood agar (SBA) medium, for a 48 hour time period, at a temperature of 37°C.”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
@@ Line 232: / Line 235: @@
 Image:CutaneousA56.jpg| Micrograph revealing submucosal hemorrhage in the small intestine, in a case of fatal human anthrax; H&E stain; Mg. 240X.”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA57.jpg| Female patient shown here on the 24th day of a Bacillus anthracis infection involving her left eye.” <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA57.jpg| "Meningeal infection due to Bacillus anthracis bacteria using an H&E stain” <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaenousA58.jpg| Female patient shown here on the 5th day of a Bacillus anthracis infection involving her left eye.”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA59.jpg| Female patient is shown here on the 5th day of a Bacillus anthracis infection involving her left eye.” <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA60.jpg| Symptoms of cutaneous anthrax due to B. anthracis”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA63.jpg| "Blood agar culture plate growing B. anthracis and other soil flora.” <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA61.jpg| Symptoms of cutaneous anthrax due to B. anthracis.” <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA62.jpg| Blood agar culture plate growing B. anthracis and other soil flora.”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA63.jpg| Hematoxylin-eosin stained (H&E) stained photomicrograph revealing some of the histopathologic changes associated with this Bacillus anthracis bacterial infection.” <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
 Image:CutaneousA65.jpg| Brain section through the ventricles revealing an inter ventricular hemorrhage as consequence of virulence factors of Bacillus anthracis (edema toxin, lethal toxin, and antiphagocytic capsular antigen). The toxins are responsible for the primary clinical manifestations of hemorrhage, edema, and necrosis”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
@@ Line 253: / Line 246: @@
 Image:CutaneousA68.jpg| Cutaneous anthrax lesion on the arm of a 50 year old female.”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA69.jpg| High magnification of 12,483X, this scanning electron micrograph (SEM) depicted spores from the Sterne strain of Bacillus anthracis bacteria.”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
 Image:CutaneousA70.jpg| "Magnification of 12,483X, this scanning electron micrograph (SEM) depicted spores from the Sterne strain of Bacillus anthracis bacteria. ”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
@@ Line 271: / Line 262: @@
 Image:CutaneousA77.jpg| "Photomicrograph demonstrating a positive Gram stain with Bacillus anthracis”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:Milzbrand.jpg| "Anthrax lesion on the skin of the forearm caused by the bacterium Bacillus anthracis”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA79.jpg| "Anthrax lesion on the neck”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:Anthrax-6.jpg| "Anthrax lesion on volar surface of right forearm”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
 Image:CutaneousA80.jpg| "Bacillus anthracis tenacity positive on sheep blood agar.”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
@@ Line 330: / Line 315: @@
 Image:CutaneousA105.jpg| "Numerous rod-shaped Bacillus anthracis bacteria, some that had linked together to form chains, while others remained solitary”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA106.jpg| "Anthrax, skin of left forearm”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA108.jpg| "Necrosis of lymph node due to anthrax”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA120.jpg| "Bacillus anthracis from agar culture <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA121.jpg| "Photomicrograph of meninges demonstrating the presence of Bacillus anthracis in a case of fatal inhalation anthrax.”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA122.jpg| "Photomicrograph of lung tissue demonstrating pneumonia in a case of fatal human inhalation anthrax<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA123.jpg| "Bacillus anthracis in lung <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA124.jpg| "Meningeal hemorrhage due to anthrax”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA107.jpg| "Anthrax, skin of right forearm, 4th day”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA125.jpg| "Photomicrograph of meninges demonstrating hemorrhage due to fatal inhalation anthrax”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA108.jpg| "Necrosis of lymph node due to anthrax”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA126.jpg| "Severe hemorrhagic necrosis of lymph node due to anthrax”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA127.jpg| "Mild meningitis with hemorrhage due to Bacillus anthracis”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA128.jpg| "Photomicrograph of serohemorrhagic meningitis in a case of fatal human anthrax with the presence ofBacillus anthracis.”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA129.jpg| "Blood agar plate culture of Bacillus anthracis, with a positive gamma phage test”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA109.jpg| "Anthrax, skin of face, 13th day”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA130.jpg| "Sheep blood agar plate culture of Bacillus anthracis and Bacillus cereus”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA110.jpg| "Anthrax, skin of face, 11th day”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA131.jpg| "Gross pathology of fixed, cut brain showing hemorrhagic meningitis due to inhalation anthrax.”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA111.jpg| "Anthrax, skin of face, 8th day”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA132.jpg| "Gross pathology of fixed, cut brain showing hemorrhagic meningitis due to inhalation anthrax.”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA112.jpg| "Anthrax, skin of face, 6th day”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA135.jpg| "Gram-stained photomicrograph revealing numerous rod-shaped Bacillus anthracis bacteria that had linked together to form long filamentous chains.” <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA113.jpg| "Anthrax, skin of face, 4th day”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA136.jpg| "Gram-stained photomicrograph revealing numerous rod-shaped Bacillus anthracis bacteria that had linked together to form long filamentous chains.” <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA114.jpg| "Anthrax, skin of right forearm, 7th day.”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA137.jpg| "Hematoxylin-eosin (H&E)-stained photomicrograph of a small intestinal tissue sample revealed the presence of histopathologic changes indicative of marked mucosal and submucosal hemorrhage, with accompanying arteriolar degeneration in a case of fatal human anthrax.” <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA115.jpg| "Anthrax, skin of right forearm, 5th day”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA138.jpg| "Hematoxylin-eosin (H&E)-stained photomicrograph of a meningeal tissue sample revealed the presence of histopathologic changes indicative of hemorrhagic meningitis in a case of fatal human anthrax.”  <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA116.jpg| "”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA139.jpg| "Histopathology of mediastinal lymph node in fatal human anthrax” <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA117.jpg| "”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA140.jpg| "Histopathology of mediastinal lymph node in fatal human anthrax” <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA118.jpg| "”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA141.jpg| "Hematoxylin-eosin (H&E)-stained photomicrograph of a mediastinal lymph node tissue sample revealed the presence of histopathologic changes indicative of an occluded blood vessel in a case of fatal human anthrax.” <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA119.jpg| "”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA142.jpg| "Hematoxylin-eosin (H&E)-stained photomicrograph of a mediastinal lymph node tissue sample revealed the presence of histopathologic changes indicative of medullary hemorrhage and necrosis in a case of fatal human anthrax.<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA119.jpg| "”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA144.jpg| "Hematoxylin-eosin (H&E)-stained photomicrograph of a mediastinal lymph node tissue sample revealed the presence of histopathologic changes indicative of medullary necrosis in a case of fatal human anthrax.” <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
-Image:CutaneousA120.jpg| "”<SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
+Image:CutaneousA145.jpg| "Under a relatively-low magnification of 96X, this hematoxylin-eosin (H&E)-stained photomicrograph of a mediastinal lymph node tissue sample revealed the presence of histopathologic changes indicative of medullary necrosis in a case of fatal human anthrax.” <SMALL><SMALL>''[http://phil.cdc.gov/phil/  Adapted from Public Health Image Library (PHIL), Centers for Disease Control and Prevention.]''<ref name="PHIL">{{Cite web | title = Public Health Image Library (PHIL), Centers for Disease Control and Prevention | url = http://phil.cdc.gov/phil/}}</ref></SMALL></SMALL>
 </gallery>
@@ Line 375: / Line 376: @@
 [[Category:Zoonoses]]
 [[Category:Medical disasters]]
+[[Category:Emergency mdicine]]
+[[Category:Up-To-Date]]
 [[Category:Infectious disease]]
+[[Category:Dermatology]]
+[[Category:Pulmonology]]
+[[Category:Gastroenterology]]

Anthrax pathophysiology: Difference between revisions

Latest revision as of 20:25, 29 July 2020

Overview

Genetics

Transmission

Pathogenesis

Bacterial Toxins

Cutaneous or Injection Anthrax

Inhalation Anthrax

Gastrointestinal Anthrax

Gross Pathology

Cutaneous and Injection Anthrax

Inhalational Anthrax

Gastrointestinal Anthrax

Microscopic Pathology

Cutaneous or Injection Anthrax

Inhalation Anthrax

Gastrointestinal Anthrax

Gallery

References

Navigation menu