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m (→‎Clinical significance: task, replaced: Journal of rheumatology → Journal of Rheumatology)
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The proteasomes form a pivotal component for the [[proteasome|Ubiquitin-Proteasome System (UPS)]] <ref>{{cite journal | vauthors = Kleiger G, Mayor T | title = Perilous journey: a tour of the ubiquitin-proteasome system | journal = Trends in Cell Biology | volume = 24 | issue = 6 | pages = 352–9 | date = Jun 2014 | pmid = 24457024 | pmc = 4037451 | doi = 10.1016/j.tcb.2013.12.003 }}</ref> and corresponding cellular Protein Quality Control (PQC). Protein [[ubiquitination]] and subsequent [[proteolysis]] and degradation by the proteasome are important mechanisms in the regulation of the [[cell cycle]], [[cell growth]] and differentiation, gene transcription, signal transduction and [[apoptosis]].<ref>{{cite journal|last1=Goldberg|first1=AL|last2=Stein|first2=R|last3=Adams|first3=J|title=New insights into proteasome function: from archaebacteria to drug development.|journal=Chemistry & Biology|date=August 1995|volume=2|issue=8|pages=503–8|pmid=9383453|doi=10.1016/1074-5521(95)90182-5}}</ref> Subsequently, a compromised proteasome complex assembly and function lead to reduced proteolytic activities and the accumulation of damaged or misfolded protein species. Such protein accumulation may contribute to the pathogenesis and phenotypic characteristics in neurodegenerative diseases,<ref>{{cite journal | vauthors = Sulistio YA, Heese K | title = The Ubiquitin-Proteasome System and Molecular Chaperone Deregulation in Alzheimer's Disease | journal = Molecular Neurobiology | date = Jan 2015 | pmid = 25561438 | doi = 10.1007/s12035-014-9063-4 | volume=53 | pages=905–31}}</ref><ref>{{cite journal | vauthors = Ortega Z, Lucas JJ | title = Ubiquitin-proteasome system involvement in Huntington's disease | journal = Frontiers in Molecular Neuroscience | volume = 7 | pages = 77 | date = 2014 | pmid = 25324717 | pmc = 4179678 | doi = 10.3389/fnmol.2014.00077 }}</ref> cardiovascular diseases,<ref>{{cite journal | vauthors = Sandri M, Robbins J | title = Proteotoxicity: an underappreciated pathology in cardiac disease | journal = Journal of Molecular and Cellular Cardiology | volume = 71 | pages = 3–10 | date = Jun 2014 | pmid = 24380730 | pmc = 4011959 | doi = 10.1016/j.yjmcc.2013.12.015 }}</ref><ref>{{cite journal | vauthors = Drews O, Taegtmeyer H | title = Targeting the ubiquitin-proteasome system in heart disease: the basis for new therapeutic strategies | journal = Antioxidants & Redox Signaling | volume = 21 | issue = 17 | pages = 2322–43 | date = Dec 2014 | pmid = 25133688 | pmc = 4241867 | doi = 10.1089/ars.2013.5823 }}</ref><ref>{{cite journal | vauthors = Wang ZV, Hill JA | title = Protein quality control and metabolism: bidirectional control in the heart | journal = Cell Metabolism | volume = 21 | issue = 2 | pages = 215–26 | date = Feb 2015 | pmid = 25651176 | pmc = 4317573 | doi = 10.1016/j.cmet.2015.01.016 }}</ref> inflammatory responses and autoimmune diseases,<ref>{{cite journal|pmid=10723801|year=2000|author1=Karin|first1=M|title=The I kappa B kinase (IKK) and NF-kappa B: Key elements of proinflammatory signalling|journal=Seminars in Immunology|volume=12|issue=1|pages=85–98|last2=Delhase|first2=M|doi=10.1006/smim.2000.0210}}</ref>  and systemic DNA damage responses leading to [[malignancies]].<ref>{{cite journal | vauthors = Ermolaeva MA, Dakhovnik A, Schumacher B | title = Quality control mechanisms in cellular and systemic DNA damage responses | journal = Ageing Research Reviews | volume = 23 | issue = Pt A | pages = 3–11 | date = Jan 2015 | pmid = 25560147 | doi = 10.1016/j.arr.2014.12.009 | pmc=4886828}}</ref>
The proteasomes form a pivotal component for the [[proteasome|Ubiquitin-Proteasome System (UPS)]] <ref>{{cite journal | vauthors = Kleiger G, Mayor T | title = Perilous journey: a tour of the ubiquitin-proteasome system | journal = Trends in Cell Biology | volume = 24 | issue = 6 | pages = 352–9 | date = Jun 2014 | pmid = 24457024 | pmc = 4037451 | doi = 10.1016/j.tcb.2013.12.003 }}</ref> and corresponding cellular Protein Quality Control (PQC). Protein [[ubiquitination]] and subsequent [[proteolysis]] and degradation by the proteasome are important mechanisms in the regulation of the [[cell cycle]], [[cell growth]] and differentiation, gene transcription, signal transduction and [[apoptosis]].<ref>{{cite journal|last1=Goldberg|first1=AL|last2=Stein|first2=R|last3=Adams|first3=J|title=New insights into proteasome function: from archaebacteria to drug development.|journal=Chemistry & Biology|date=August 1995|volume=2|issue=8|pages=503–8|pmid=9383453|doi=10.1016/1074-5521(95)90182-5}}</ref> Subsequently, a compromised proteasome complex assembly and function lead to reduced proteolytic activities and the accumulation of damaged or misfolded protein species. Such protein accumulation may contribute to the pathogenesis and phenotypic characteristics in neurodegenerative diseases,<ref>{{cite journal | vauthors = Sulistio YA, Heese K | title = The Ubiquitin-Proteasome System and Molecular Chaperone Deregulation in Alzheimer's Disease | journal = Molecular Neurobiology | date = Jan 2015 | pmid = 25561438 | doi = 10.1007/s12035-014-9063-4 | volume=53 | pages=905–31}}</ref><ref>{{cite journal | vauthors = Ortega Z, Lucas JJ | title = Ubiquitin-proteasome system involvement in Huntington's disease | journal = Frontiers in Molecular Neuroscience | volume = 7 | pages = 77 | date = 2014 | pmid = 25324717 | pmc = 4179678 | doi = 10.3389/fnmol.2014.00077 }}</ref> cardiovascular diseases,<ref>{{cite journal | vauthors = Sandri M, Robbins J | title = Proteotoxicity: an underappreciated pathology in cardiac disease | journal = Journal of Molecular and Cellular Cardiology | volume = 71 | pages = 3–10 | date = Jun 2014 | pmid = 24380730 | pmc = 4011959 | doi = 10.1016/j.yjmcc.2013.12.015 }}</ref><ref>{{cite journal | vauthors = Drews O, Taegtmeyer H | title = Targeting the ubiquitin-proteasome system in heart disease: the basis for new therapeutic strategies | journal = Antioxidants & Redox Signaling | volume = 21 | issue = 17 | pages = 2322–43 | date = Dec 2014 | pmid = 25133688 | pmc = 4241867 | doi = 10.1089/ars.2013.5823 }}</ref><ref>{{cite journal | vauthors = Wang ZV, Hill JA | title = Protein quality control and metabolism: bidirectional control in the heart | journal = Cell Metabolism | volume = 21 | issue = 2 | pages = 215–26 | date = Feb 2015 | pmid = 25651176 | pmc = 4317573 | doi = 10.1016/j.cmet.2015.01.016 }}</ref> inflammatory responses and autoimmune diseases,<ref>{{cite journal|pmid=10723801|year=2000|author1=Karin|first1=M|title=The I kappa B kinase (IKK) and NF-kappa B: Key elements of proinflammatory signalling|journal=Seminars in Immunology|volume=12|issue=1|pages=85–98|last2=Delhase|first2=M|doi=10.1006/smim.2000.0210}}</ref>  and systemic DNA damage responses leading to [[malignancies]].<ref>{{cite journal | vauthors = Ermolaeva MA, Dakhovnik A, Schumacher B | title = Quality control mechanisms in cellular and systemic DNA damage responses | journal = Ageing Research Reviews | volume = 23 | issue = Pt A | pages = 3–11 | date = Jan 2015 | pmid = 25560147 | doi = 10.1016/j.arr.2014.12.009 | pmc=4886828}}</ref>


Several experimental and clinical studies have indicated that aberrations and deregulations of the UPS contribute to the pathogenesis of several neurodegenerative and myodegenerative disorders, including [[Alzheimer's disease]],<ref>{{cite journal|last1=Checler|first1=F|last2=da Costa|first2=CA|last3=Ancolio|first3=K|last4=Chevallier|first4=N|last5=Lopez-Perez|first5=E|last6=Marambaud|first6=P|title=Role of the proteasome in Alzheimer's disease.|journal=Biochimica et Biophysica Acta|date=26 July 2000|volume=1502|issue=1|pages=133–8|pmid=10899438|doi=10.1016/s0925-4439(00)00039-9}}</ref> [[Parkinson's disease]]<ref name="ReferenceC">{{cite journal|last1=Chung|first1=KK|last2=Dawson|first2=VL|last3=Dawson|first3=TM|title=The role of the ubiquitin-proteasomal pathway in Parkinson's disease and other neurodegenerative disorders.|journal=Trends in Neurosciences|date=November 2001|volume=24|issue=11 Suppl|pages=S7–14|pmid=11881748|doi=10.1016/s0166-2236(00)01998-6}}</ref> and [[Pick's disease]],<ref name="IkedaAkiyama2002">{{cite journal|last1=Ikeda|first1=K|last2=Akiyama|first2=H|last3=Arai|first3=T|last4=Ueno|first4=H|last5=Tsuchiya|first5=K|last6=Kosaka|first6=K|title=Morphometrical reappraisal of motor neuron system of Pick's disease and amyotrophic lateral sclerosis with dementia.|journal=Acta Neuropathologica|date=July 2002|volume=104|issue=1|pages=21–8|pmid=12070660|doi=10.1007/s00401-001-0513-5}}</ref> [[Amyotrophic lateral sclerosis]] ([[ALS]]),<ref name="IkedaAkiyama2002"/> [[Huntington's disease]],<ref name="ReferenceC"/> [[Creutzfeldt–Jakob disease]],<ref>{{cite journal|last1=Manaka|first1=H|last2=Kato|first2=T|last3=Kurita|first3=K|last4=Katagiri|first4=T|last5=Shikama|first5=Y|last6=Kujirai|first6=K|last7=Kawanami|first7=T|last8=Suzuki|first8=Y|last9=Nihei|first9=K|last10=Sasaki|first10=H|title=Marked increase in cerebrospinal fluid ubiquitin in Creutzfeldt–Jakob disease.|journal=Neuroscience Letters|date=11 May 1992|volume=139|issue=1|pages=47–9|pmid=1328965|doi=10.1016/0304-3940(92)90854-z}}</ref> and motor neuron diseases, polyglutamine (PolyQ) diseases, [[Muscular dystrophies]]<ref>{{cite journal|last1=Mathews|first1=KD|last2=Moore|first2=SA|title=Limb-girdle muscular dystrophy.|journal=Current Neurology and Neuroscience Reports|date=January 2003|volume=3|issue=1|pages=78–85|pmid=12507416|doi=10.1007/s11910-003-0042-9}}</ref> and several rare forms of neurodegenerative diseases associated with [[dementia]].<ref>{{cite journal|last1=Mayer|first1=RJ|title=From neurodegeneration to neurohomeostasis: the role of ubiquitin.|journal=Drug news & perspectives|date=March 2003|volume=16|issue=2|pages=103–8|pmid=12792671|doi=10.1358/dnp.2003.16.2.829327}}</ref> As part of the [[proteasome|Ubiquitin-Proteasome System (UPS)]], the proteasome maintains cardiac protein homeostasis and thus plays a significant role in cardiac [[Ischemic]] injury,<ref>{{cite journal|pmid=23220331|pmc=3774499|year=2013|author1=Calise|first1=J|title=The ubiquitin proteasome system and myocardial ischemia|journal=AJP: Heart and Circulatory Physiology|volume=304|issue=3|pages=H337–49|last2=Powell|first2=S. R.|doi=10.1152/ajpheart.00604.2012}}</ref> [[ventricular hypertrophy]]<ref>{{cite journal|last1=Predmore|first1=JM|last2=Wang|first2=P|last3=Davis|first3=F|last4=Bartolone|first4=S|last5=Westfall|first5=MV|last6=Dyke|first6=DB|last7=Pagani|first7=F|last8=Powell|first8=SR|last9=Day|first9=SM|title=Ubiquitin proteasome dysfunction in human hypertrophic and dilated cardiomyopathies.|journal=Circulation|date=2 March 2010|volume=121|issue=8|pages=997–1004|pmid=20159828|doi=10.1161/circulationaha.109.904557|pmc=2857348}}</ref> and [[Heart failure]].<ref>{{cite journal|last1=Powell|first1=SR|title=The ubiquitin-proteasome system in cardiac physiology and pathology|journal=American Journal of Physiology. Heart and Circulatory Physiology|date=July 2006|volume=291|issue=1|pages=H1–H19|pmid=16501026|doi=10.1152/ajpheart.00062.2006}}</ref> Additionally, evidence is accumulating that the UPS plays an essential role in malignant transformation. UPS proteolysis plays a major role in responses of cancer cells to stimulatory signals that are critical for the development of cancer. Accordingly, gene expression by degradation of [[transcription factors]], such as [[p53]], [[c-Jun]], [[c-Fos]], [[NF-κB]], [[c-Myc]], HIF-1α, MATα2, [[STAT3]], sterol-regulated element-binding proteins and [[androgen receptors]] are all controlled by the UPS and thus involved in the development of various malignancies.<ref>{{cite journal|last1=Adams|first1=J|title=Potential for proteasome inhibition in the treatment of cancer.|journal=Drug Discovery Today|date=1 April 2003|volume=8|issue=7|pages=307–15|pmid=12654543|doi=10.1016/s1359-6446(03)02647-3}}</ref> Moreover, the UPS regulates the degradation of tumor suppressor gene products such as [[adenomatous polyposis coli]] ([[adenomatous polyposis coli|APC]]) in colorectal cancer, [[retinoblastoma]] (Rb). and [[von Hippel-Lindau tumor suppressor]] (VHL), as well as a number of [[proto-oncogenes]] ([[Raf kinase|Raf]], [[Myc]], [[MYB (gene)|Myb]], [[NF-κB|Rel]], [[Src (gene)|Src]], [[MOS (gene)|Mos]], [[Abl (gene)|Abl]]). The UPS is also involved in the regulation of inflammatory responses. This activity is usually attributed to the role of proteasomes in the activation of NF-κB which further regulates the expression of pro inflammatory [[cytokines]] such as [[TNF-α]], IL-β, [[Interleukin 8|IL-8]], [[adhesion molecules]] ([[ICAM-1]], [[VCAM-1]], P selectine) and [[prostaglandins]] and [[nitric oxide]] (NO).<ref>{{cite journal|last1=Karin|first1=M|last2=Delhase|first2=M|title=The I kappa B kinase (IKK) and NF-kappa B: key elements of proinflammatory signalling.|journal=Seminars in immunology|date=February 2000|volume=12|issue=1|pages=85–98|pmid=10723801|doi=10.1006/smim.2000.0210}}</ref> Additionally, the UPS also plays a role in inflammatory responses as regulators of leukocyte proliferation, mainly through proteolysis of cyclines and the degradation of [[Cyclin-dependent kinase|CDK]] inhibitors.<ref>{{cite journal|last1=Ben-Neriah|first1=Y|title=Regulatory functions of ubiquitination in the immune system|journal=Nature Immunology|date=January 2002|volume=3|issue=1|pages=20–6|pmid=11753406|doi=10.1038/ni0102-20}}</ref> Lastly, [[autoimmune disease]] patients with [[Systemic lupus erythematosus|SLE]], [[Sjogren's syndrome]] and [[rheumatoid arthritis]] (RA) predominantly exhibit circulating proteasomes which can be applied as clinical biomarkers.<ref>{{cite journal|last1=Egerer|first1=K|last2=Kuckelkorn|first2=U|last3=Rudolph|first3=PE|last4=Rückert|first4=JC|last5=Dörner|first5=T|last6=Burmester|first6=GR|last7=Kloetzel|first7=PM|last8=Feist|first8=E|title=Circulating proteasomes are markers of cell damage and immunologic activity in autoimmune diseases.|journal=The Journal of rheumatology|date=October 2002|volume=29|issue=10|pages=2045–52|pmid=12375310}}</ref>
Several experimental and clinical studies have indicated that aberrations and deregulations of the UPS contribute to the pathogenesis of several neurodegenerative and myodegenerative disorders, including [[Alzheimer's disease]],<ref>{{cite journal|last1=Checler|first1=F|last2=da Costa|first2=CA|last3=Ancolio|first3=K|last4=Chevallier|first4=N|last5=Lopez-Perez|first5=E|last6=Marambaud|first6=P|title=Role of the proteasome in Alzheimer's disease.|journal=Biochimica et Biophysica Acta|date=26 July 2000|volume=1502|issue=1|pages=133–8|pmid=10899438|doi=10.1016/s0925-4439(00)00039-9}}</ref> [[Parkinson's disease]]<ref name="ReferenceC">{{cite journal|last1=Chung|first1=KK|last2=Dawson|first2=VL|last3=Dawson|first3=TM|title=The role of the ubiquitin-proteasomal pathway in Parkinson's disease and other neurodegenerative disorders.|journal=Trends in Neurosciences|date=November 2001|volume=24|issue=11 Suppl|pages=S7–14|pmid=11881748|doi=10.1016/s0166-2236(00)01998-6}}</ref> and [[Pick's disease]],<ref name="IkedaAkiyama2002">{{cite journal|last1=Ikeda|first1=K|last2=Akiyama|first2=H|last3=Arai|first3=T|last4=Ueno|first4=H|last5=Tsuchiya|first5=K|last6=Kosaka|first6=K|title=Morphometrical reappraisal of motor neuron system of Pick's disease and amyotrophic lateral sclerosis with dementia.|journal=Acta Neuropathologica|date=July 2002|volume=104|issue=1|pages=21–8|pmid=12070660|doi=10.1007/s00401-001-0513-5}}</ref> [[Amyotrophic lateral sclerosis]] ([[ALS]]),<ref name="IkedaAkiyama2002"/> [[Huntington's disease]],<ref name="ReferenceC"/> [[Creutzfeldt–Jakob disease]],<ref>{{cite journal|last1=Manaka|first1=H|last2=Kato|first2=T|last3=Kurita|first3=K|last4=Katagiri|first4=T|last5=Shikama|first5=Y|last6=Kujirai|first6=K|last7=Kawanami|first7=T|last8=Suzuki|first8=Y|last9=Nihei|first9=K|last10=Sasaki|first10=H|title=Marked increase in cerebrospinal fluid ubiquitin in Creutzfeldt–Jakob disease.|journal=Neuroscience Letters|date=11 May 1992|volume=139|issue=1|pages=47–9|pmid=1328965|doi=10.1016/0304-3940(92)90854-z}}</ref> and motor neuron diseases, polyglutamine (PolyQ) diseases, [[Muscular dystrophies]]<ref>{{cite journal|last1=Mathews|first1=KD|last2=Moore|first2=SA|title=Limb-girdle muscular dystrophy.|journal=Current Neurology and Neuroscience Reports|date=January 2003|volume=3|issue=1|pages=78–85|pmid=12507416|doi=10.1007/s11910-003-0042-9}}</ref> and several rare forms of neurodegenerative diseases associated with [[dementia]].<ref>{{cite journal|last1=Mayer|first1=RJ|title=From neurodegeneration to neurohomeostasis: the role of ubiquitin.|journal=Drug news & perspectives|date=March 2003|volume=16|issue=2|pages=103–8|pmid=12792671|doi=10.1358/dnp.2003.16.2.829327}}</ref> As part of the [[proteasome|Ubiquitin-Proteasome System (UPS)]], the proteasome maintains cardiac protein homeostasis and thus plays a significant role in cardiac [[Ischemic]] injury,<ref>{{cite journal|pmid=23220331|pmc=3774499|year=2013|author1=Calise|first1=J|title=The ubiquitin proteasome system and myocardial ischemia|journal=AJP: Heart and Circulatory Physiology|volume=304|issue=3|pages=H337–49|last2=Powell|first2=S. R.|doi=10.1152/ajpheart.00604.2012}}</ref> [[ventricular hypertrophy]]<ref>{{cite journal|last1=Predmore|first1=JM|last2=Wang|first2=P|last3=Davis|first3=F|last4=Bartolone|first4=S|last5=Westfall|first5=MV|last6=Dyke|first6=DB|last7=Pagani|first7=F|last8=Powell|first8=SR|last9=Day|first9=SM|title=Ubiquitin proteasome dysfunction in human hypertrophic and dilated cardiomyopathies.|journal=Circulation|date=2 March 2010|volume=121|issue=8|pages=997–1004|pmid=20159828|doi=10.1161/circulationaha.109.904557|pmc=2857348}}</ref> and [[Heart failure]].<ref>{{cite journal|last1=Powell|first1=SR|title=The ubiquitin-proteasome system in cardiac physiology and pathology|journal=American Journal of Physiology. Heart and Circulatory Physiology|date=July 2006|volume=291|issue=1|pages=H1–H19|pmid=16501026|doi=10.1152/ajpheart.00062.2006}}</ref> Additionally, evidence is accumulating that the UPS plays an essential role in malignant transformation. UPS proteolysis plays a major role in responses of cancer cells to stimulatory signals that are critical for the development of cancer. Accordingly, gene expression by degradation of [[transcription factors]], such as [[p53]], [[c-Jun]], [[c-Fos]], [[NF-κB]], [[c-Myc]], HIF-1α, MATα2, [[STAT3]], sterol-regulated element-binding proteins and [[androgen receptors]] are all controlled by the UPS and thus involved in the development of various malignancies.<ref>{{cite journal|last1=Adams|first1=J|title=Potential for proteasome inhibition in the treatment of cancer.|journal=Drug Discovery Today|date=1 April 2003|volume=8|issue=7|pages=307–15|pmid=12654543|doi=10.1016/s1359-6446(03)02647-3}}</ref> Moreover, the UPS regulates the degradation of tumor suppressor gene products such as [[adenomatous polyposis coli]] ([[adenomatous polyposis coli|APC]]) in colorectal cancer, [[retinoblastoma]] (Rb). and [[von Hippel-Lindau tumor suppressor]] (VHL), as well as a number of [[proto-oncogenes]] ([[Raf kinase|Raf]], [[Myc]], [[MYB (gene)|Myb]], [[NF-κB|Rel]], [[Src (gene)|Src]], [[MOS (gene)|Mos]], [[Abl (gene)|Abl]]). The UPS is also involved in the regulation of inflammatory responses. This activity is usually attributed to the role of proteasomes in the activation of NF-κB which further regulates the expression of pro inflammatory [[cytokines]] such as [[TNF-α]], IL-β, [[Interleukin 8|IL-8]], [[adhesion molecules]] ([[ICAM-1]], [[VCAM-1]], [[P-selectin]]) and [[prostaglandins]] and [[nitric oxide]] (NO).<ref>{{cite journal|last1=Karin|first1=M|last2=Delhase|first2=M|title=The I kappa B kinase (IKK) and NF-kappa B: key elements of proinflammatory signalling.|journal=Seminars in immunology|date=February 2000|volume=12|issue=1|pages=85–98|pmid=10723801|doi=10.1006/smim.2000.0210}}</ref> Additionally, the UPS also plays a role in inflammatory responses as regulators of leukocyte proliferation, mainly through proteolysis of cyclines and the degradation of [[Cyclin-dependent kinase|CDK]] inhibitors.<ref>{{cite journal|last1=Ben-Neriah|first1=Y|title=Regulatory functions of ubiquitination in the immune system|journal=Nature Immunology|date=January 2002|volume=3|issue=1|pages=20–6|pmid=11753406|doi=10.1038/ni0102-20}}</ref> Lastly, [[autoimmune disease]] patients with [[Systemic lupus erythematosus|SLE]], [[Sjogren's syndrome]] and [[rheumatoid arthritis]] (RA) predominantly exhibit circulating proteasomes which can be applied as clinical biomarkers.<ref>{{cite journal|last1=Egerer|first1=K|last2=Kuckelkorn|first2=U|last3=Rudolph|first3=PE|last4=Rückert|first4=JC|last5=Dörner|first5=T|last6=Burmester|first6=GR|last7=Kloetzel|first7=PM|last8=Feist|first8=E|title=Circulating proteasomes are markers of cell damage and immunologic activity in autoimmune diseases.|journal=The Journal of Rheumatology|date=October 2002|volume=29|issue=10|pages=2045–52|pmid=12375310}}</ref>


Radiation therapy is a critical modality in the treatment of cancer. Accordingly, the proteasome subunit alpha type-1 was examined as a strategy in radio sensitizing for the treatment of [[non-small cell lung carcinomas]]. Proteasome inhibition through the knockdown of [[PSMA1]] resulting in loss of protein expression of the proteasome subunit alpha type-1 and the proteasome chymotrypsin-like activity and also in a loss of expression of PSMB5 protein (proteasome subunit beta type-5). A combination of PSMA1 knockdown in parallel with [[radiation therapy]] to treat non-small cell lung carcinoma resulted in an increased sensitivity of the tumor to radiation and improved tumor control.<ref>{{cite journal|last1=Cron|first1=KR|last2=Zhu|first2=K|last3=Kushwaha|first3=DS|last4=Hsieh|first4=G|last5=Merzon|first5=D|last6=Rameseder|first6=J|last7=Chen|first7=CC|last8=D'Andrea|first8=AD|last9=Kozono|first9=D|title=Proteasome inhibitors block DNA repair and radiosensitize non-small cell lung cancer|journal=PLOS ONE|date=2013|volume=8|issue=9|pages=e73710|pmid=24040035|bibcode=2013PLoSO...873710C|doi=10.1371/journal.pone.0073710|pmc=3764058}}</ref> The study suggests that proteasome inhibition through PSMA1 knockdown is a promising strategy for non-small cell lung carcinomas radiosensitization via inhibition of NF-κB-mediated expression of [[Fanconi Anemia]]/HR DNA repair genes, and that the proteasome subunit beta type-5 may play a significant role in this process.<ref>{{cite journal|last1=Cron|first1=KR|last2=Zhu|first2=K|last3=Kushwaha|first3=DS|last4=Hsieh|first4=G|last5=Merzon|first5=D|last6=Rameseder|first6=J|last7=Chen|first7=CC|last8=D'Andrea|first8=AD|last9=Kozono|first9=D|title=Proteasome inhibitors block DNA repair and radiosensitize non-small cell lung cancer|journal=PLOS ONE|date=2013|volume=8|issue=9|pages=e73710|pmid=24040035|bibcode=2013PLoSO...873710C|doi=10.1371/journal.pone.0073710|pmc=3764058}}</ref>
Radiation therapy is a critical modality in the treatment of cancer. Accordingly, the proteasome subunit alpha type-1 was examined as a strategy in radio sensitizing for the treatment of [[non-small cell lung carcinomas]]. Proteasome inhibition through the knockdown of [[PSMA1]] resulting in loss of protein expression of the proteasome subunit alpha type-1 and the proteasome chymotrypsin-like activity and also in a loss of expression of PSMB5 protein (proteasome subunit beta type-5). A combination of PSMA1 knockdown in parallel with [[radiation therapy]] to treat non-small cell lung carcinoma resulted in an increased sensitivity of the tumor to radiation and improved tumor control.<ref name="ReferenceD">{{cite journal|last1=Cron|first1=KR|last2=Zhu|first2=K|last3=Kushwaha|first3=DS|last4=Hsieh|first4=G|last5=Merzon|first5=D|last6=Rameseder|first6=J|last7=Chen|first7=CC|last8=D'Andrea|first8=AD|last9=Kozono|first9=D|title=Proteasome inhibitors block DNA repair and radiosensitize non-small cell lung cancer|journal=PLOS ONE|date=2013|volume=8|issue=9|pages=e73710|pmid=24040035|bibcode=2013PLoSO...873710C|doi=10.1371/journal.pone.0073710|pmc=3764058}}</ref> The study suggests that proteasome inhibition through PSMA1 knockdown is a promising strategy for non-small cell lung carcinomas radiosensitization via inhibition of NF-κB-mediated expression of [[Fanconi Anemia]]/HR DNA repair genes, and that the proteasome subunit beta type-5 may play a significant role in this process.<ref name="ReferenceD"/>


==References==
==References==

Revision as of 00:11, 16 October 2018

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Identifiers
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External IDsGeneCards: [1]
Orthologs
SpeciesHumanMouse
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RefSeq (protein)

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Proteasome subunit beta type-5 as known as 20S proteasome subunit beta-5 is a protein that in humans is encoded by the PSMB5 gene.[1][2][3] This protein is one of the 17 essential subunits (alpha subunits 1–7, constitutive beta subunits 1–7, and inducible subunits including beta1i, beta2i, beta5i) that contributes to the complete assembly of 20S proteasome complex. In particular, proteasome subunit beta type-5, along with other beta subunits, assemble into two heptameric rings and subsequently a proteolytic chamber for substrate degradation. This protein contains "chymotrypsin-like" activity and is capable of cleaving after large hydrophobic residues of peptide.[2] The eukaryotic proteasome recognized degradable proteins, including damaged proteins for protein quality control purpose or key regulatory protein components for dynamic biological processes. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.

Structure

Protein expression

The gene PSMB5 encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit beta5i (proteasome beta 8 subunit).[3] The gene has 5 exons and locates at chromosome band 14q11.2. The human protein proteasome subunit beta type-5 is 22 kDa in size and composed of 204 amino acids. The calculated theoretical pI of this protein is 8.66.

Complex assembly

The proteasome is a multicatalytic proteinase complex with a highly ordered 20S core structure. This barrel-shaped core structure is composed of 4 axially stacked rings of 28 non-identical subunits: the two end rings are each formed by 7 alpha subunits, and the two central rings are each formed by 7 beta subunits. Three beta subunits (beta1, beta2, and beta5) each contains a proteolytic active site and has distinct substrate preferences. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.[4][5]

Function

Protein functions are supported by its tertiary structure and its interaction with associating partners. As one of 28 subunits of 20S proteasome, protein proteasome subunit beta type-2 contributes to form a proteolytic environment for substrate degradation. Evidences of the crystal structures of isolated 20S proteasome complex demonstrate that the two rings of beta subunits form a proteolytic chamber and maintain all their active sites of proteolysis within the chamber.[5] Concomitantly, the rings of alpha subunits form the entrance for substrates entering the proteolytic chamber. In an inactivated 20S proteasome complex, the gate into the internal proteolytic chamber are guarded by the N-terminal tails of specific alpha-subunit. This unique structure design prevents random encounter between proteolytic active sites and protein substrate, which makes protein degradation a well-regulated process.[6][7] 20S proteasome complex, by itself, is usually functionally inactive. The proteolytic capacity of 20S core particle (CP) can be activated when CP associates with one or two regulatory particles (RP) on one or both side of alpha rings. These regulatory particles include 19S proteasome complexes, 11S proteasome complex, etc. Following the CP-RP association, the confirmation of certain alpha subunits will change and consequently cause the opening of substrate entrance gate. Besides RPs, the 20S proteasomes can also be effectively activated by other mild chemical treatments, such as exposure to low levels of sodium dodecylsulfate (SDS) or NP-14.[7][8]

The 20S proteasome subunit beta-5 (systematic nomenclature) is originally expressed as a precursor with 263 amino acids. The fragment of 59 amino acids at peptide N-terminal is essential for proper protein folding and subsequent complex assembly. At the end-stage of complex assembly, the N-terminal fragment of beta5 subunit is cleaved, forming the mature beta5 subunit of 20S complex.[9]

Clinical significance

The Proteasome and its subunits are of clinical significance for at least two reasons: (1) a compromised complex assembly or a dysfunctional proteasome can be associated with the underlying pathophysiology of specific diseases, and (2) they can be exploited as drug targets for therapeutic interventions. More recently, more effort has been made to consider the proteasome for the development of novel diagnostic markers and strategies. An improved and comprehensive understanding of the pathophysiology of the proteasome should lead to clinical applications in the future.

The proteasomes form a pivotal component for the Ubiquitin-Proteasome System (UPS) [10] and corresponding cellular Protein Quality Control (PQC). Protein ubiquitination and subsequent proteolysis and degradation by the proteasome are important mechanisms in the regulation of the cell cycle, cell growth and differentiation, gene transcription, signal transduction and apoptosis.[11] Subsequently, a compromised proteasome complex assembly and function lead to reduced proteolytic activities and the accumulation of damaged or misfolded protein species. Such protein accumulation may contribute to the pathogenesis and phenotypic characteristics in neurodegenerative diseases,[12][13] cardiovascular diseases,[14][15][16] inflammatory responses and autoimmune diseases,[17] and systemic DNA damage responses leading to malignancies.[18]

Several experimental and clinical studies have indicated that aberrations and deregulations of the UPS contribute to the pathogenesis of several neurodegenerative and myodegenerative disorders, including Alzheimer's disease,[19] Parkinson's disease[20] and Pick's disease,[21] Amyotrophic lateral sclerosis (ALS),[21] Huntington's disease,[20] Creutzfeldt–Jakob disease,[22] and motor neuron diseases, polyglutamine (PolyQ) diseases, Muscular dystrophies[23] and several rare forms of neurodegenerative diseases associated with dementia.[24] As part of the Ubiquitin-Proteasome System (UPS), the proteasome maintains cardiac protein homeostasis and thus plays a significant role in cardiac Ischemic injury,[25] ventricular hypertrophy[26] and Heart failure.[27] Additionally, evidence is accumulating that the UPS plays an essential role in malignant transformation. UPS proteolysis plays a major role in responses of cancer cells to stimulatory signals that are critical for the development of cancer. Accordingly, gene expression by degradation of transcription factors, such as p53, c-Jun, c-Fos, NF-κB, c-Myc, HIF-1α, MATα2, STAT3, sterol-regulated element-binding proteins and androgen receptors are all controlled by the UPS and thus involved in the development of various malignancies.[28] Moreover, the UPS regulates the degradation of tumor suppressor gene products such as adenomatous polyposis coli (APC) in colorectal cancer, retinoblastoma (Rb). and von Hippel-Lindau tumor suppressor (VHL), as well as a number of proto-oncogenes (Raf, Myc, Myb, Rel, Src, Mos, Abl). The UPS is also involved in the regulation of inflammatory responses. This activity is usually attributed to the role of proteasomes in the activation of NF-κB which further regulates the expression of pro inflammatory cytokines such as TNF-α, IL-β, IL-8, adhesion molecules (ICAM-1, VCAM-1, P-selectin) and prostaglandins and nitric oxide (NO).[29] Additionally, the UPS also plays a role in inflammatory responses as regulators of leukocyte proliferation, mainly through proteolysis of cyclines and the degradation of CDK inhibitors.[30] Lastly, autoimmune disease patients with SLE, Sjogren's syndrome and rheumatoid arthritis (RA) predominantly exhibit circulating proteasomes which can be applied as clinical biomarkers.[31]

Radiation therapy is a critical modality in the treatment of cancer. Accordingly, the proteasome subunit alpha type-1 was examined as a strategy in radio sensitizing for the treatment of non-small cell lung carcinomas. Proteasome inhibition through the knockdown of PSMA1 resulting in loss of protein expression of the proteasome subunit alpha type-1 and the proteasome chymotrypsin-like activity and also in a loss of expression of PSMB5 protein (proteasome subunit beta type-5). A combination of PSMA1 knockdown in parallel with radiation therapy to treat non-small cell lung carcinoma resulted in an increased sensitivity of the tumor to radiation and improved tumor control.[32] The study suggests that proteasome inhibition through PSMA1 knockdown is a promising strategy for non-small cell lung carcinomas radiosensitization via inhibition of NF-κB-mediated expression of Fanconi Anemia/HR DNA repair genes, and that the proteasome subunit beta type-5 may play a significant role in this process.[32]

References

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Further reading