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{{Clostridium difficile infection}}
{{Taxobox
{{Taxobox
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| binomial_authority = Hall & O'Toole, 1935
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{{Siren|Clostridium difficile infection}}
{{Siren|Clostridium difficile infection}}  
<span style="color: red;">'''For more details about the conditions that are associated with the development of ''C. difficile'' infection (e.g. list of antibiotics), click [[Clostridium difficile risk factors|here]].'''</span><br>
{{Clostridium difficile infection}}
{{About0|Clostridium difficile infection}}
 
{{CMG}}
<span style="color:red">'''For more details about the conditions that are associated with the development of ''C. difficile'' infection (e.g. list of antibiotics), click [[Clostridium difficile risk factors|here]]'''</span><br>
 
{{CMG}}; {{AE}} {{YD}}
==Overview==
==Overview==
''C. difficile'' infection is caused by ''Clostridium difficile'', a spore-forming, toxin-producing, oligate anaerobic, gram-positive bacillus.
''C. difficile'' infection is caused by ''Clostridium difficile'', a spore-forming, toxin-producing, obligate anaerobic, gram-positive bacillus.
 
'''''Clostridium difficile''''' ([[#Etymology and pronunciation|etymology and pronunciation]]), also known as '''''C. difficile''', '''C. diff''' ({{IPAc-en|s|iː}} {{IPAc-en|d|ɪ|f}}), or sometimes '''CDF/cdf''', is a [[species]] of [[Gram-positive]] [[Bacterial spore|spore]]-forming [[bacteria]].<ref name="Moreno - June 2013">{{cite journal | author = Moreno MA, Furtner F, Rivara FP | title = Clostridium difficile: A Cause of Diarrhea in Children | journal = JAMA Pediatrics | volume = 167 | issue = 6 | page = 592 | date = June 2013 | pmid = 23733223 | doi = 10.1001/.jamapediatrics.2013.2551 | url = http://archpedi.jamanetwork.com/article.aspx?articleid=1695335 | doi_brokendate = 2015-01-11 | last2 = Furtner | last3 = Rivara }}</ref>
 
Clostridia (members of the genus ''[[Clostridium]]'') are [[Anaerobic organism|anaerobic]], [[Motility|motile]] [[bacteria]], ubiquitous in nature, and especially prevalent in soil. Under the microscope, they appear as long, irregular (often drumstick- or spindle-shaped) cells with a bulge at their terminal ends. Under [[Gram stain]]ing, ''C. difficile'' cells are [[Gram-positive]] and show optimum growth on [[blood agar]] at human body temperatures in the absence of [[oxygen]]. When stressed, the bacteria produce [[Bacterial spore|spore]]s that are able to tolerate extreme conditions that the active bacteria cannot tolerate.<ref name="Sherris">{{cite book | author = Ryan KJ, Ray CG (editors) | title = Sherris Medical Microbiology | edition = 4th | pages = 322–4 | publisher = McGraw Hill | year = 2004 | isbn= 0-8385-8529-9 }}</ref>
 
''C. difficile'' may become established in the human [[large intestine|colon]]; it is present in 2–5% of the adult population.<ref name=Sherris/>


==Organism==
* Clostridium difficile ([[#Etymology and pronunciation|etymology and pronunciation]]), also known as '''''C. difficile''', '''C. diff''', or sometimes '''CDF/cdf''', is a [[species]] of [[Gram-positive]] [[Bacterial spore|spore]]-forming [[bacteria]].<ref name="Moreno - June 2013">{{cite journal | author = Moreno MA, Furtner F, Rivara FP | title = Clostridium difficile: A Cause of Diarrhea in Children | journal = JAMA Pediatrics | volume = 167 | issue = 6 | page = 592 | date = June 2013 | pmid = 23733223 | doi = 10.1001/.jamapediatrics.2013.2551 | url = http://archpedi.jamanetwork.com/article.aspx?articleid=1695335 | doi_brokendate = 2015-01-11 | last2 = Furtner | last3 = Rivara }}</ref>
* Clostridia (members of the genus ''[[Clostridium]]'') are [[Anaerobic organism|anaerobic]], [[Motility|motile]] [[bacteria]], ubiquitous in nature, and especially prevalent in soil. Under the microscope, they appear as long, irregular (often drumstick- or spindle-shaped) cells with a bulge at their terminal ends. Under [[Gram stain]]ing, ''C. difficile'' cells are [[Gram-positive]] and show optimum growth on [[blood agar]] at human body temperatures in the absence of [[oxygen]]. When stressed, the bacteria produce [[Bacterial spore|spore]]s that are able to tolerate extreme conditions that the active bacteria cannot tolerate.<ref name="Sherris">{{cite book | author = Ryan KJ, Ray CG (editors) | title = Sherris Medical Microbiology | edition = 4th | pages = 322–4 | publisher = McGraw Hill | year = 2004 | isbn= 0-8385-8529-9 }}</ref>
* C. difficile may become established in the human [[large intestine|colon]]; it is present in 2–5% of the adult population.<ref name=Sherris/>
== Human pathogen ==
== Human pathogen ==
* Pathogenic ''C. difficile'' strains produce multiple [[toxin]]s. The most well-characterized are [[enterotoxin]] ([[Clostridium difficile toxin A|''Clostridium difficile'' toxin A]]) and [[cytotoxin]] ([[Clostridium difficile toxin B|''Clostridium difficile'' toxin B]]), both of which may produce [[diarrhea]] and [[inflammation]] in infected patients ([[Clostridium difficile colitis|''Clostridium difficile'' colitis]]), although their relative contributions have been debated.<ref name=Sherris /> Toxins A and B are glucosyltransferases that target and inactivate the [[Rho family of GTPases]]. Toxin B (cytotoxin) induces [[actin]] depolymerization by a mechanism correlated with a decrease in the [[ADP-ribosylation]] of the low molecular mass GTP-binding Rho proteins.<ref>{{cite journal | author = Just I, Selzer J, von Eichel-Streiber C, Aktories K | title = The low molecular mass GTP-binding protein Rh is affected by toxin a from ''Clostridium difficile'' | journal = The Journal of Clinical Investigation | volume = 95 | issue = 3 | pages = 1026–31 | year = 1995 | pmid = 7883950 | pmc = 441436 | doi = 10.1172/JCI117747 | last2 = Selzer | last3 = von Eichel-Streiber | last4 = Aktories }}</ref>
* Another toxin, [[Pore-forming toxin#Binary toxins|binary toxin]], also has been described, but its role in disease is not fully understood.<ref>{{cite journal | author = Barth H, Aktories K, Popoff MR, Stiles BG | title = Binary Bacterial Toxins: Biochemistry, Biology, and Applications of Common Clostridium and Bacillus Proteins | journal = Microbiology and Molecular Biology Reviews : MMBR | volume = 68 | issue = 3 | pages = 373–402, table of contents | year = 2004 | pmid = 15353562 | pmc = 515256 | doi = 10.1128/MMBR.68.3.373-402.2004 | last2 = Aktories | last3 = Popoff | last4 = Stiles }}</ref>
* [[Antibiotic treatment]] of ''C. diff'' infections may be difficult, due both to [[antibiotic resistance]] and physiological factors of the bacteria (spore formation, protective effects of the pseudomembrane).<ref name=Sherris /> The emergence of a new, highly toxic strain of ''C. difficile'', resistant to [[fluoroquinolone]] antibiotics, such as [[ciprofloxacin]] and [[levofloxacin]], said to be causing geographically dispersed outbreaks in North America, was reported in 2005.<ref name=Loo_2005>{{cite journal | author = Loo VG, Poirier L, Miller MA, Oughton M, Libman MD, Michaud S, Bourgault AM, Nguyen T, Frenette C, Kelly M, Vibien A, Brassard P, Fenn S, Dewar K, Hudson TJ, Horn R, René P, Monczak Y, Dascal A | title = A predominantly clonal multi-institutional outbreak of Clostridium difficile-associated diarrhea with high morbidity and mortality | journal = The New England Journal of Medicine | volume = 353 | issue = 23 | pages = 2442–9 | year = 2005 month = December | pmid = 16322602 | doi = 10.1056/NEJMoa051639 | last2 = Poirier | last3 = Miller | last4 = Oughton | last5 = Libman | last6 = Michaud | last7 = Bourgault | last8 = Nguyen | last9 = Frenette | last10 = Kelly | last11 = Vibien | last12 = Brassard | last13 = Fenn | last14 = Dewar | last15 = Hudson | last16 = Horn | last17 = René | last18 = Monczak | last19 = Dascal }}</ref>
* C. difficile is transmitted from person to person by the [[fecal-oral route]]. However, the organism forms heat-resistant spores that are not killed by alcohol-based hand cleansers or routine surface cleaning. Thus, these spores survive in clinical environments for long periods. Because of this, the bacteria may be cultured from almost any surface. Once spores are ingested, their acid-resistance allows them to pass through the stomach unscathed. They germinate and multiply into vegetative cells in the colon upon exposure to bile acids.
== Strains ==
* In 2005, molecular analysis led to the identification of the ''C. difficile'' strain type characterized as group BI by [[Restriction enzyme|restriction endonuclease]] analysis<!-- (REA) -->, as North American pulse-field-type NAP1 by [[pulsed-field gel electrophoresis]] and as [[ribotyping|ribotype]] 027; the differing terminology reflects the predominant techniques used for epidemiological typing. This strain is referred to as ''C. difficile'' BI/NAP1/027.<ref name=Rupnik_2009>{{cite journal | author = Rupnik M, Wilcox MH, Gerding DN | title = Clostridium difficile infection: New developments in epidemiology and pathogenesis | journal = Nature Reviews. Microbiology | volume = 7 | issue = 7 | pages = 526–36 | date = July 2009 | pmid = 19528959 | doi = 10.1038/nrmicro2164 | last2 = Wilcox | last3 = Gerding }}</ref>
==Genome==
* The first complete genome sequence of a ''C. difficile'' strain was first published in 2005 by [[Sanger Institute]] in the UK. This was of the strain 630, a virulent and multiple drug-resistant strain isolated in Switzerland in 1982. Scientists at Sanger Institute have sequenced genomes of about 30 ''C. difficile'' isolates using next-generation [[sequencing]] technologies from [[454 Life Sciences]] and [[Illumina (company)|Illumina]].<ref name="pmid20368420">{{cite journal | author = He M, Sebaihia M, Lawley TD, Stabler RA, Dawson LF, Martin MJ, Holt KE, Seth-Smith HM, Quail MA, Rance R, Brooks K, Churcher C, Harris D, Bentley SD, Burrows C, Clark L, Corton C, Murray V, Rose G, Thurston S, van Tonder A, Walker D, Wren BW, Dougan G, Parkhill J | title = Evolutionary dynamics of'' Clostridium difficile ''over short and long time scales | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 107 | issue = 16 | pages = 7527–32 | date = April 2010 | pmid = 20368420 | pmc = 2867753 | doi = 10.1073/pnas.0914322107 | url = http://www.pnas.org/content/107/16/7527.full.pdf | last2 = Sebaihia | last3 = Lawley | last4 = Stabler | last5 = Dawson | last6 = Martin | last7 = Holt | last8 = Seth-Smith | last9 = Quail | last10 = Rance | last11 = Brooks | last12 = Churcher | last13 = Harris | last14 = Bentley | last15 = Burrows | last16 = Clark | last17 = Corton | last18 = Murray | last19 = Rose | last20 = Thurston | last21 = Van Tonder | last22 = Walker | last23 = Wren | last24 = Dougan | last25 = Parkhill | bibcode = 2010PNAS..107.7527H }}</ref>
* Researchers at [[McGill University]] in Montreal sequenced the [[genome]] of the highly virulent Quebec strain of ''C. difficile'' in 2005 using ultra-high-throughput sequencing technology. The tests involved doing 400,000 DNA parallel-sequencing reactions of the bacterium's genome, which had been fragmented for sequencing. These sequences were assembled computationally to form a complete genome sequence.<ref name=Loo_2005 /><ref>[http://www.iapm.ca/newsmanager/anmviewer.asp?a=144&z=14 Scientists map C. difficile strain] - Institute of Public Affairs, Montreal</ref>
* In 2012, scientists at University of Oxford sequenced ''C. difficile'' genomes from 486 cases arising over four years in Oxfordshire using next-generation sequencing technologies from Illumina.<ref name="pmid23259504">{{cite journal | author = Didelot X, Eyre DW, Cule M, Ip CL, Ansari MA, Griffiths D, Vaughan A, O'Connor L, Golubchik T, Batty EM, Piazza P, Wilson DJ, Bowden R, Donnelly PJ, Dingle KE, Wilcox M, Walker AS, Crook DW, A Peto TE, Harding RM | title = Microevolutionary analysis of Clostridium difficile genomes to investigate transmission | journal = Genome Biology | volume = 13 | issue = 12 | pages = R118 | date = December 2012 | pmid = 23259504 | pmc = 4056369 | doi = 10.1186/gb-2012-13-12-r118 | url = http://genomebiology.com/content/pdf/gb-2012-13-12-r118.pdf | last2 = Eyre | last3 = Cule | last4 = Ip | last5 = Ansari | last6 = Griffiths | last7 = Vaughan | last8 = O'Connor | last9 = Golubchik | last10 = Batty | last11 = Piazza | last12 = Wilson | last13 = Bowden | last14 = Donnelly | last15 = Dingle | last16 = Wilcox | last17 = Walker | last18 = Crook | last19 = Peto | last20 = Harding }}</ref>


Pathogenic ''C. difficile'' strains produce multiple [[toxin]]s. The most well-characterized are [[enterotoxin]] ([[Clostridium difficile toxin A|''Clostridium difficile'' toxin A]]) and [[cytotoxin]] ([[Clostridium difficile toxin B|''Clostridium difficile'' toxin B]]), both of which may produce [[diarrhea]] and [[inflammation]] in infected patients ([[Clostridium difficile colitis|''Clostridium difficile'' colitis]]), although their relative contributions have been debated.<ref name=Sherris /> Toxins A and B are glucosyltransferases that target and inactivate the [[Rho family of GTPases]]. Toxin B (cytotoxin) induces [[actin]] depolymerization by a mechanism correlated with a decrease in the [[ADP-ribosylation]] of the low molecular mass GTP-binding Rho proteins.<ref>{{cite journal | author = Just I, Selzer J, von Eichel-Streiber C, Aktories K | title = The low molecular mass GTP-binding protein Rh is affected by toxin a from ''Clostridium difficile'' | journal = The Journal of Clinical Investigation | volume = 95 | issue = 3 | pages = 1026–31 | year = 1995 | pmid = 7883950 | pmc = 441436 | doi = 10.1172/JCI117747 | last2 = Selzer | last3 = von Eichel-Streiber | last4 = Aktories }}</ref> Another toxin, [[Pore-forming toxin#Binary toxins|binary toxin]], also has been described, but its role in disease is not fully understood.<ref>{{cite journal | author = Barth H, Aktories K, Popoff MR, Stiles BG | title = Binary Bacterial Toxins: Biochemistry, Biology, and Applications of Common Clostridium and Bacillus Proteins | journal = Microbiology and Molecular Biology Reviews : MMBR | volume = 68 | issue = 3 | pages = 373–402, table of contents | year = 2004 | pmid = 15353562 | pmc = 515256 | doi = 10.1128/MMBR.68.3.373-402.2004 | last2 = Aktories | last3 = Popoff | last4 = Stiles }}</ref>
== Bacteriophage ==
 
* At least eight mainly temperate [[bacteriophage]]s have been isolated from ''C. difficile'', ranging in genome size from about 30 to about 60 kb.<ref name="Hargreaves">{{cite journal | author = Hargreaves KR, Clokie MR | title = ''Clostridium difficile'' phages: Still difficult? | journal = Frontiers in Microbiology | volume = 5 | pages = 184 | year = 2014 | pmid = 24808893 | pmc = 4009436 | doi = 10.3389/fmicb.2014.00184 | last2 = Clokie }}</ref> Both environmentally and clinically derived&nbsp;''C. difficile''&nbsp;strains carry a diverse and prevalent set of [[prophage]]s.<ref name="Hargreaves"/>
[[Antibiotic treatment]] of ''C. diff'' infections may be difficult, due both to [[antibiotic resistance]] and physiological factors of the bacteria (spore formation, protective effects of the pseudomembrane).<ref name=Sherris /> The emergence of a new, highly toxic strain of ''C. difficile'', resistant to [[fluoroquinolone]] antibiotics, such as [[ciprofloxacin]] and [[levofloxacin]], said to be causing geographically dispersed outbreaks in North America, was reported in 2005.<ref name=Loo_2005>{{cite journal | author = Loo VG, Poirier L, Miller MA, Oughton M, Libman MD, Michaud S, Bourgault AM, Nguyen T, Frenette C, Kelly M, Vibien A, Brassard P, Fenn S, Dewar K, Hudson TJ, Horn R, René P, Monczak Y, Dascal A | title = A predominantly clonal multi-institutional outbreak of Clostridium difficile-associated diarrhea with high morbidity and mortality | journal = The New England Journal of Medicine | volume = 353 | issue = 23 | pages = 2442–9 | year = 2005 month = December | pmid = 16322602 | doi = 10.1056/NEJMoa051639 | last2 = Poirier | last3 = Miller | last4 = Oughton | last5 = Libman | last6 = Michaud | last7 = Bourgault | last8 = Nguyen | last9 = Frenette | last10 = Kelly | last11 = Vibien | last12 = Brassard | last13 = Fenn | last14 = Dewar | last15 = Hudson | last16 = Horn | last17 = René | last18 = Monczak | last19 = Dascal }}</ref> The U.S. [[Centers for Disease Control]] <!-- (CDC) --> in Atlanta warned of the emergence of an epidemic strain with increased virulence, antibiotic resistance, or both.<ref name=McDonald_2005>{{cite journal | author = McDonald LC | title = Clostridium difficile: responding to a new threat from an old enemy | journal = Infection Control and Hospital Epidemiology : The Official Journal of the Society of Hospital Epidemiologists of America | volume = 26 | issue = 8 | pages = 672–5 | date = August 2005 | pmid = 16156321 | doi = 10.1086/502600 | url = http://www.cdc.gov/ncidod/dhqp/pdf/infDis/Cdiff_ICHE08_05.pdf }}</ref>
==Etymology and pronunciation==
 
* The species name is [[New Latin]], from the [[Greek (language)|Greek]] and [[Latin]] ''difficile'', "difficult, obstinate".<ref>{{Cite journal| last=Cawley |first=Kevin |title=Difficilis |work=Latin Dictionary and Grammar Aid |publisher=[[University of Notre Dame]] |url=http://www.archives.nd.edu/cgi-bin/lookup.pl?stem=Difficilis&ending= |accessdate=2013-03-16 |postscript=&#123;&#123;inconsistent citations&#125;&#125; }}</ref>.
''C. difficile'' is transmitted from person to person by the [[fecal-oral route]]. However, the organism forms heat-resistant spores that are not killed by alcohol-based hand cleansers or routine surface cleaning. Thus, these spores survive in clinical environments for long periods. Because of this, the bacteria may be cultured from almost any surface. Once spores are ingested, their acid-resistance allows them to pass through the stomach unscathed. They germinate and multiply into vegetative cells in the colon upon exposure to bile acids.
 
A 2015 CDC study estimated that ''C. diff'' afflicted almost half a million Americans and caused 29,000 deaths in 2011. The study estimated that 40 percent of cases began in nursing homes or community health care settings, while 24 percent occurred in hospitals.<ref>{{cite news |title=Death Toll From Bacteria Is Raised |first= PAM |last=BELLUCKFEB |date= February 25, 2015 |accessdate=February 2015 |publisher=[[The New York Times]]}}</ref>


== Strains ==
==Cause of Clostridium difficile infection==
* C. difficile infection is caused by Clostridium difficile. C. difficile has the following characteristic features:
* Bacillus
* Gram-positive
* Obligate anaerobe
* Spore-forming (spores are acid-, heat-, and antibiotic-resistant)
* Toxin-producing (exotoxins A and B)
* Motile<br>
* C. difficile is difficult to isolate and grow in cultures. Because the organism is very sensitive to oxygen (obligate anaerobe), spore formation is essential for the organism to be transmitted from one host to another.
==Gallery==
<gallery>
Image: Clostridium difficile09.jpeg| Clostridium difficile enterotoxin, CPE, is the principal toxin involved in C. difficile foodborne illness. <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL>
Image: Clostridium difficile07.jpeg| Gram-positive C. difficile bacteria from a stool sample culture obtained using a .1µm filter. <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL>


In 2005, molecular analysis led to the identification of the ''C. difficile'' strain type characterized as group BI by [[Restriction enzyme|restriction endonuclease]] analysis<!-- (REA) -->, as North American pulse-field-type NAP1 by [[pulsed-field gel electrophoresis]] <!-- (PFGE) --> and as [[ribotyping|ribotype]] 027; the differing terminology reflects the predominant techniques used for epidemiological typing. This strain is referred to as ''C. difficile'' BI/NAP1/027.<ref name=Rupnik_2009>{{cite journal | author = Rupnik M, Wilcox MH, Gerding DN | title = Clostridium difficile infection: New developments in epidemiology and pathogenesis | journal = Nature Reviews. Microbiology | volume = 7 | issue = 7 | pages = 526–36 | date = July 2009 | pmid = 19528959 | doi = 10.1038/nrmicro2164 | last2 = Wilcox | last3 = Gerding }}</ref>
Image: Clostridium difficile06.jpeg| Gram-positive C. difficile bacteria from a stool sample culture obtained using a .1µm filter. <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL>


==Genome==
Image: Clostridium difficile05.jpeg| Gram-positive C. difficile bacteria from a stool sample culture obtained using a .1µm filter. <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL>
{{Infobox genome
| image =
| caption =
| taxId = 535?
| ploidy = haploid
| chromosomes = 1
| size = 4.3 Mb
| year = 2005
}}
The first complete genome sequence of a ''C. difficile'' strain was first published in 2005 by [[Sanger Institute]] in the UK. This was of the strain 630, a virulent and multiple drug-resistant strain isolated in Switzerland in 1982. Scientists at Sanger Institute have sequenced genomes of about 30 ''C. difficile'' isolates using next-generation [[sequencing]] technologies from [[454 Life Sciences]] and [[Illumina (company)|Illumina]].<ref name="pmid20368420">{{cite journal | author = He M, Sebaihia M, Lawley TD, Stabler RA, Dawson LF, Martin MJ, Holt KE, Seth-Smith HM, Quail MA, Rance R, Brooks K, Churcher C, Harris D, Bentley SD, Burrows C, Clark L, Corton C, Murray V, Rose G, Thurston S, van Tonder A, Walker D, Wren BW, Dougan G, Parkhill J | title = Evolutionary dynamics of'' Clostridium difficile ''over short and long time scales | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 107 | issue = 16 | pages = 7527–32 | date = April 2010 | pmid = 20368420 | pmc = 2867753 | doi = 10.1073/pnas.0914322107 | url = http://www.pnas.org/content/107/16/7527.full.pdf | last2 = Sebaihia | last3 = Lawley | last4 = Stabler | last5 = Dawson | last6 = Martin | last7 = Holt | last8 = Seth-Smith | last9 = Quail | last10 = Rance | last11 = Brooks | last12 = Churcher | last13 = Harris | last14 = Bentley | last15 = Burrows | last16 = Clark | last17 = Corton | last18 = Murray | last19 = Rose | last20 = Thurston | last21 = Van Tonder | last22 = Walker | last23 = Wren | last24 = Dougan | last25 = Parkhill | bibcode = 2010PNAS..107.7527H }}</ref>


Researchers at [[McGill University]] in Montreal sequenced the [[genome]] of the highly virulent Quebec strain of ''C. difficile'' in 2005 using ultra-high-throughput sequencing technology. The tests involved doing 400,000 DNA parallel-sequencing reactions of the bacterium's genome, which had been fragmented for sequencing. These sequences were assembled computationally to form a complete genome sequence.<ref name=Loo_2005 /><ref>[http://www.iapm.ca/newsmanager/anmviewer.asp?a=144&z=14 Scientists map C. difficile strain] - Institute of Public Affairs, Montreal</ref>


In 2012, scientists at University of Oxford sequenced ''C. difficile'' genomes from 486 cases arising over four years in Oxfordshire using next-generation sequencing technologies from Illumina.<ref name="pmid23259504">{{cite journal | author = Didelot X, Eyre DW, Cule M, Ip CL, Ansari MA, Griffiths D, Vaughan A, O'Connor L, Golubchik T, Batty EM, Piazza P, Wilson DJ, Bowden R, Donnelly PJ, Dingle KE, Wilcox M, Walker AS, Crook DW, A Peto TE, Harding RM | title = Microevolutionary analysis of Clostridium difficile genomes to investigate transmission | journal = Genome Biology | volume = 13 | issue = 12 | pages = R118 | date = December 2012 | pmid = 23259504 | pmc = 4056369 | doi = 10.1186/gb-2012-13-12-r118 | url = http://genomebiology.com/content/pdf/gb-2012-13-12-r118.pdf | last2 = Eyre | last3 = Cule | last4 = Ip | last5 = Ansari | last6 = Griffiths | last7 = Vaughan | last8 = O'Connor | last9 = Golubchik | last10 = Batty | last11 = Piazza | last12 = Wilson | last13 = Bowden | last14 = Donnelly | last15 = Dingle | last16 = Wilcox | last17 = Walker | last18 = Crook | last19 = Peto | last20 = Harding }}</ref>
Image: Clostridium difficile04.jpeg| Gram-positive C. difficile bacteria from a stool sample culture obtained using a .1µm filter. <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL>


== Bacteriophage ==
Image: Clostridium difficile03.jpeg| Gram-positive C. difficile bacteria from a stool sample culture obtained using a .1µm filter. <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL>
At least eight mainly temperate [[bacteriophage]]s have been isolated from ''C. difficile'', ranging in genome size from about 30 to about 60 kb.<ref name="Hargreaves">{{cite journal | author = Hargreaves KR, Clokie MR | title = ''Clostridium difficile'' phages: Still difficult? | journal = Frontiers in Microbiology | volume = 5 | pages = 184 | year = 2014 | pmid = 24808893 | pmc = 4009436 | doi = 10.3389/fmicb.2014.00184 | last2 = Clokie }}</ref> Both environmentally and clinically derived&nbsp;''C. difficile''&nbsp;strains carry a diverse and prevalent set of [[prophage]]s.<ref name="Hargreaves"/>


==Etymology and pronunciation==
Image: Clostridium difficile02.jpeg| Illustration depicts the ultrastructural morphology exhibited by a single Gram-positive Clostridium difficile bacillus. <SMALL><SMALL>''[http://phil.cdc.gov/phil/home.asp From Public Health Image Library (PHIL).] ''<ref name=PHIL> {{Cite web | title = Public Health Image Library (PHIL) | url = http://phil.cdc.gov/phil/home.asp}}</ref></SMALL></SMALL>
The species name is [[New Latin]], from the [[Greek (language)|Greek]] ''kloster'' (κλωστήρ), "spindle",<ref>{{Cite journal|last=Liddell-Scott |title= κλωστήρ |work=Greek-English Lexicon |publisher=Oxford|postscript= &#123;&#123;inconsistent citations&#125;&#125; }}</ref> and [[Latin]] ''difficile'', "difficult, obstinate".<ref>{{Cite journal| last=Cawley |first=Kevin |title=Difficilis |work=Latin Dictionary and Grammar Aid |publisher=[[University of Notre Dame]] |url=http://www.archives.nd.edu/cgi-bin/lookup.pl?stem=Difficilis&ending= |accessdate=2013-03-16 |postscript=&#123;&#123;inconsistent citations&#125;&#125; }}</ref> It is pronounced {{IPAc-en|k|l|ɔː|ˈ|s|t|r|ɪ|d|i|əm}} {{IPAc-en|d|ɨ|ˈ|f|ɪ|s|ɨ|l|i}} in medical English (compare [[wikt:difficile#Latin|classical Latin /difˈfi.ki.leː/]]), although [[spelling pronunciation]]s such as {{IPAc-en|ˈ|d|ɪ|f|ɨ|s|i|l}} are commonly heard.


</gallery>
==References==
==References==
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Latest revision as of 17:25, 18 September 2017

C. difficile Infection Microchapters

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Clostridium difficile
C. difficile colonies on a blood agar plate
C. difficile colonies on a blood agar plate
 Micrograph of Clostridium difficile
Micrograph of Clostridium difficile
Scientific classification
Kingdom: Bacteria
Phylum: Firmicutes
Class: Clostridia
Order: Clostridiales
Family: Clostridiaceae
Genus: Clostridium
Species: C. difficile
Binomial name
Clostridium difficile
Hall & O'Toole, 1935
Resident
Survival
Guide

For more details about the conditions that are associated with the development of C. difficile infection (e.g. list of antibiotics), click here.

This page is about microbiologic aspects of the organism(s).  For clinical aspects of the disease, see Clostridium difficile infection.

Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]

Overview

C. difficile infection is caused by Clostridium difficile, a spore-forming, toxin-producing, obligate anaerobic, gram-positive bacillus.

Organism

  • Clostridium difficile (etymology and pronunciation), also known as C. difficile, C. diff, or sometimes CDF/cdf, is a species of Gram-positive spore-forming bacteria.[1]
  • Clostridia (members of the genus Clostridium) are anaerobic, motile bacteria, ubiquitous in nature, and especially prevalent in soil. Under the microscope, they appear as long, irregular (often drumstick- or spindle-shaped) cells with a bulge at their terminal ends. Under Gram staining, C. difficile cells are Gram-positive and show optimum growth on blood agar at human body temperatures in the absence of oxygen. When stressed, the bacteria produce spores that are able to tolerate extreme conditions that the active bacteria cannot tolerate.[2]
  • C. difficile may become established in the human colon; it is present in 2–5% of the adult population.[2]

Human pathogen

  • Pathogenic C. difficile strains produce multiple toxins. The most well-characterized are enterotoxin (Clostridium difficile toxin A) and cytotoxin (Clostridium difficile toxin B), both of which may produce diarrhea and inflammation in infected patients (Clostridium difficile colitis), although their relative contributions have been debated.[2] Toxins A and B are glucosyltransferases that target and inactivate the Rho family of GTPases. Toxin B (cytotoxin) induces actin depolymerization by a mechanism correlated with a decrease in the ADP-ribosylation of the low molecular mass GTP-binding Rho proteins.[3]
  • Another toxin, binary toxin, also has been described, but its role in disease is not fully understood.[4]
  • Antibiotic treatment of C. diff infections may be difficult, due both to antibiotic resistance and physiological factors of the bacteria (spore formation, protective effects of the pseudomembrane).[2] The emergence of a new, highly toxic strain of C. difficile, resistant to fluoroquinolone antibiotics, such as ciprofloxacin and levofloxacin, said to be causing geographically dispersed outbreaks in North America, was reported in 2005.[5]
  • C. difficile is transmitted from person to person by the fecal-oral route. However, the organism forms heat-resistant spores that are not killed by alcohol-based hand cleansers or routine surface cleaning. Thus, these spores survive in clinical environments for long periods. Because of this, the bacteria may be cultured from almost any surface. Once spores are ingested, their acid-resistance allows them to pass through the stomach unscathed. They germinate and multiply into vegetative cells in the colon upon exposure to bile acids.

Strains

  • In 2005, molecular analysis led to the identification of the C. difficile strain type characterized as group BI by restriction endonuclease analysis, as North American pulse-field-type NAP1 by pulsed-field gel electrophoresis and as ribotype 027; the differing terminology reflects the predominant techniques used for epidemiological typing. This strain is referred to as C. difficile BI/NAP1/027.[6]

Genome

  • The first complete genome sequence of a C. difficile strain was first published in 2005 by Sanger Institute in the UK. This was of the strain 630, a virulent and multiple drug-resistant strain isolated in Switzerland in 1982. Scientists at Sanger Institute have sequenced genomes of about 30 C. difficile isolates using next-generation sequencing technologies from 454 Life Sciences and Illumina.[7]
  • Researchers at McGill University in Montreal sequenced the genome of the highly virulent Quebec strain of C. difficile in 2005 using ultra-high-throughput sequencing technology. The tests involved doing 400,000 DNA parallel-sequencing reactions of the bacterium's genome, which had been fragmented for sequencing. These sequences were assembled computationally to form a complete genome sequence.[5][8]
  • In 2012, scientists at University of Oxford sequenced C. difficile genomes from 486 cases arising over four years in Oxfordshire using next-generation sequencing technologies from Illumina.[9]

Bacteriophage

  • At least eight mainly temperate bacteriophages have been isolated from C. difficile, ranging in genome size from about 30 to about 60 kb.[10] Both environmentally and clinically derived C. difficile strains carry a diverse and prevalent set of prophages.[10]

Etymology and pronunciation

Cause of Clostridium difficile infection

  • C. difficile infection is caused by Clostridium difficile. C. difficile has the following characteristic features:
  • Bacillus
  • Gram-positive
  • Obligate anaerobe
  • Spore-forming (spores are acid-, heat-, and antibiotic-resistant)
  • Toxin-producing (exotoxins A and B)
  • Motile
  • C. difficile is difficult to isolate and grow in cultures. Because the organism is very sensitive to oxygen (obligate anaerobe), spore formation is essential for the organism to be transmitted from one host to another.

Gallery

References

  1. ↑ Moreno MA, Furtner F, Rivara FP; Furtner; Rivara (June 2013). "Clostridium difficile: A Cause of Diarrhea in Children". JAMA Pediatrics. 167 (6): 592. doi:10.1001/.jamapediatrics.2013.2551. PMID 23733223. Unknown parameter |doi_brokendate= ignored (help)
  2. ↑ 2.0 2.1 2.2 2.3 Ryan KJ, Ray CG (editors) (2004). Sherris Medical Microbiology (4th ed.). McGraw Hill. pp. 322–4. ISBN 0-8385-8529-9.
  3. ↑ Just I, Selzer J, von Eichel-Streiber C, Aktories K; Selzer; von Eichel-Streiber; Aktories (1995). "The low molecular mass GTP-binding protein Rh is affected by toxin a from Clostridium difficile". The Journal of Clinical Investigation. 95 (3): 1026–31. doi:10.1172/JCI117747. PMC 441436. PMID 7883950.
  4. ↑ Barth H, Aktories K, Popoff MR, Stiles BG; Aktories; Popoff; Stiles (2004). "Binary Bacterial Toxins: Biochemistry, Biology, and Applications of Common Clostridium and Bacillus Proteins". Microbiology and Molecular Biology Reviews : MMBR. 68 (3): 373–402, table of contents. doi:10.1128/MMBR.68.3.373-402.2004. PMC 515256. PMID 15353562.
  5. ↑ 5.0 5.1 Loo VG, Poirier L, Miller MA, Oughton M, Libman MD, Michaud S, Bourgault AM, Nguyen T, Frenette C, Kelly M, Vibien A, Brassard P, Fenn S, Dewar K, Hudson TJ, Horn R, René P, Monczak Y, Dascal A; Poirier; Miller; Oughton; Libman; Michaud; Bourgault; Nguyen; Frenette; Kelly; Vibien; Brassard; Fenn; Dewar; Hudson; Horn; René; Monczak; Dascal (2005 month = December). "A predominantly clonal multi-institutional outbreak of Clostridium difficile-associated diarrhea with high morbidity and mortality". The New England Journal of Medicine. 353 (23): 2442–9. doi:10.1056/NEJMoa051639. PMID 16322602. Check date values in: |year= (help)
  6. ↑ Rupnik M, Wilcox MH, Gerding DN; Wilcox; Gerding (July 2009). "Clostridium difficile infection: New developments in epidemiology and pathogenesis". Nature Reviews. Microbiology. 7 (7): 526–36. doi:10.1038/nrmicro2164. PMID 19528959.
  7. ↑ He M, Sebaihia M, Lawley TD, Stabler RA, Dawson LF, Martin MJ, Holt KE, Seth-Smith HM, Quail MA, Rance R, Brooks K, Churcher C, Harris D, Bentley SD, Burrows C, Clark L, Corton C, Murray V, Rose G, Thurston S, van Tonder A, Walker D, Wren BW, Dougan G, Parkhill J; Sebaihia; Lawley; Stabler; Dawson; Martin; Holt; Seth-Smith; Quail; Rance; Brooks; Churcher; Harris; Bentley; Burrows; Clark; Corton; Murray; Rose; Thurston; Van Tonder; Walker; Wren; Dougan; Parkhill (April 2010). "Evolutionary dynamics of Clostridium difficile over short and long time scales" (PDF). Proceedings of the National Academy of Sciences of the United States of America. 107 (16): 7527–32. Bibcode:2010PNAS..107.7527H. doi:10.1073/pnas.0914322107. PMC 2867753. PMID 20368420.
  8. ↑ Scientists map C. difficile strain - Institute of Public Affairs, Montreal
  9. ↑ Didelot X, Eyre DW, Cule M, Ip CL, Ansari MA, Griffiths D, Vaughan A, O'Connor L, Golubchik T, Batty EM, Piazza P, Wilson DJ, Bowden R, Donnelly PJ, Dingle KE, Wilcox M, Walker AS, Crook DW, A Peto TE, Harding RM; Eyre; Cule; Ip; Ansari; Griffiths; Vaughan; O'Connor; Golubchik; Batty; Piazza; Wilson; Bowden; Donnelly; Dingle; Wilcox; Walker; Crook; Peto; Harding (December 2012). "Microevolutionary analysis of Clostridium difficile genomes to investigate transmission" (PDF). Genome Biology. 13 (12): R118. doi:10.1186/gb-2012-13-12-r118. PMC 4056369. PMID 23259504.
  10. ↑ 10.0 10.1 Hargreaves KR, Clokie MR; Clokie (2014). "Clostridium difficile phages: Still difficult?". Frontiers in Microbiology. 5: 184. doi:10.3389/fmicb.2014.00184. PMC 4009436. PMID 24808893.
  11. ↑ Cawley, Kevin. "Difficilis". Latin Dictionary and Grammar Aid. University of Notre Dame. Retrieved 2013-03-16{{inconsistent citations}}
  12. ↑ 12.0 12.1 12.2 12.3 12.4 12.5 12.6 "Public Health Image Library (PHIL)".

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