Whipple's disease other diagnostic studies: Difference between revisions
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==Other Diagnostic Studies== | ==Other Diagnostic Studies== | ||
*Small bowel biopsy may be helpful in the diagnosis of Whipple's disease. Findings diagnostic of | *[[Small bowel]] [[biopsy]] may be helpful in the diagnosis of Whipple's disease. Findings diagnostic of Whipple's disease include:<ref name="DutlyAltwegg2001">{{cite journal|last1=Dutly|first1=F.|last2=Altwegg|first2=M.|title=Whipple's Disease and "Tropheryma whippelii"|journal=Clinical Microbiology Reviews|volume=14|issue=3|year=2001|pages=561–583|issn=0893-8512|doi=10.1128/CMR.14.3.561-583.2001}}</ref> | ||
**PAS positive inclusions in macrophages of lamina propria | **[[PAS stain|PAS]] positive [[inclusions]] in [[macrophages]] of [[lamina propria]] | ||
**All the organs affected by disease may | **All the organs affected by disease may demonstrate [[Periodic acid-Schiff stain|PAS]] positive [[macrophages]] like [[heart]], [[lungs]], [[CNS]], [[eyes]], [[liver]], [[spleen]], [[joints]], and [[bone marrow]]. | ||
* Other diagnostic studies for [[Whipple's disease]] which confirm the diagnosis include: | * Other diagnostic studies for [[Whipple's disease]] which confirm the diagnosis include: | ||
*[[Electron | **[[Electron microscopy]], which demonstrates: | ||
** | ***Typical trilamellar [[membrane]] of [[Tropheryma whipplei]]. | ||
** | ***Gram positive cell wall structure | ||
**[[PCR]] of affected tissue, which demonstrates: | |||
***16S [[rRNA]] gene of [[Tropheryma whipplei]] | |||
* [[ | ** Serum [[immunofluorescent]] assay, which demonstrates: | ||
** | ***[[Antibodies]] against [[Tropheryma whipplei]] | ||
* [[ | |||
==References== | ==References== | ||
Revision as of 21:18, 9 November 2017
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Bhagyashree Deshmankar M.B.B.S.[2]
Overview
Small bowel biopsy may be helpful in the diagnosis of Whipple's disease. Diagnostic findings of Whipple's disease include, PAS positive inclusions in macrophages of lamina propria. Other diagnostic studies for Whipple's disease include electron microscopy which demonstrate bacteria, immunofluorescent assay to detect antibodies against Tropheryma whipplei and PCR, which demonstrates 16S rRNA gene of Tropheryma whipplei.
Other Diagnostic Studies
- Small bowel biopsy may be helpful in the diagnosis of Whipple's disease. Findings diagnostic of Whipple's disease include:[1]
- PAS positive inclusions in macrophages of lamina propria
- All the organs affected by disease may demonstrate PAS positive macrophages like heart, lungs, CNS, eyes, liver, spleen, joints, and bone marrow.
- Other diagnostic studies for Whipple's disease which confirm the diagnosis include:
- Electron microscopy, which demonstrates:
- Typical trilamellar membrane of Tropheryma whipplei.
- Gram positive cell wall structure
- PCR of affected tissue, which demonstrates:
- 16S rRNA gene of Tropheryma whipplei
- Electron microscopy, which demonstrates:
- Serum immunofluorescent assay, which demonstrates:
- Antibodies against Tropheryma whipplei
- Serum immunofluorescent assay, which demonstrates:
References
- ↑ Dutly, F.; Altwegg, M. (2001). "Whipple's Disease and "Tropheryma whippelii"". Clinical Microbiology Reviews. 14 (3): 561–583. doi:10.1128/CMR.14.3.561-583.2001. ISSN 0893-8512.