WBR0886

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Author [[PageAuthor::Serge Korjian M.D. (Reviewed by Serge Korjian)]]
Exam Type USMLE Step 1
Main Category Pharmacology, Physiology
Sub Category Endocrine
Prompt A 53-year-old man presents to the endocrinology clinic after his primary care physician documented new-onset diabetes mellitus poorly responding to oral antidiabetics with an HbA1C of 9.3%. The patient reports adhering to a strict diet but despite this feels like he has gained much weight in the past 3 months. He also reports significant lower extremity weakness and fatigue recently. His physical exam is notable for a blood pressure of 174/89 mmHg, significant abdominal obesity, proximal muscle weakness, and fat collection at the base of neck. After a series of tests, the physician diagnoses Cushing's disease and decides to start the patient on metyrapone therapy. What would you expect to increase after the initiation of therapy?
Answer A 11-deoxycortisol
Answer A Explanation 11-deoxycortisol the substrate of 11β-hydroxylase inhibited by metyrapone accumulates after initiation of therapy.
Answer B Corticosterone
Answer B Explanation Corticosterone is one of the products of 11β-hydroxylase and would decrease rather than increase with metyrapone therapy.
Answer C Aldosterone
Answer C Explanation Aldosterone is one of the products of 11β-hydroxylase and would decrease rather than increase with metyrapone therapy.
Answer D 17-hydroxypregnenolone
Answer D Explanation 17-hydroxypregnenolone is unaffected by inhibition of 11β-hydroxylase.
Answer E Pregnenolone
Answer E Explanation Pregnenolone is unaffected by inhibition of 11β-hydroxylase.
Right Answer A
Explanation Metyrapone is an agent used mainly for diagnosing adrenal insufficiency via blocking steroid synthesis and monitoring the hypothalamic-pituitary-adrenal axis for increase in steroidogenesis. Failure of detecting an increase in ACTH or 11-deoxycortisol levels support the diagnosis of adrenal insufficiency. Metyrapone acts by inhibiting the last step in cortisol synthesis by blocking the enzyme 11β-hydroxylase. It is also used in the treatment of Cushing's disease (not very common practice) to decrease the corticoid load. Typically, if 11β-hydroxylase is inhibited, it's substrate would accumulate. After initiation of therapy, serum levels of 11-deoxycortisol would be expected to increase. Urine levels of 17-hydroxysteroids, the breakdown products of 11β-hydroxylase are also expected to increase.

Educational Objective: Metyrapone inhibits the last step in cortisol synthesis by blocking the enzyme 11β-hydroxylase.
References: Avgerinos PC, Yanovski JA, Oldfield EH, Nieman LK, Cutler GB. The metyrapone and dexamethasone suppression tests for the differential diagnosis of the adrenocorticotropin-dependent Cushing syndrome: a comparison. Ann Intern Med. 1994;121(5):318-27.
First Aid 2014 page 317

Approved Yes
Keyword Metyrapone, Cushing's disease, Cortisol, Diabetes, Adrenal insufficiency
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