Hantavirus infection classification

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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Basir Gill, M.B.B.S, M.D.[2] Usama Talib, BSc, MD [3]

Overview

Hantavirus infection can be classified based on taxonomic classification of the virus, clinical syndrome, and phylogenetic host association. The family Hantaviridae (class Bunyaviricetes, order Elliovirales) encompasses seven genera and 53 species, with all human-pathogenic hantaviruses belonging to the genus Orthohantavirus within the subfamily Mammantavirinae.[1][2] The two major clinical syndromes are hemorrhagic fever with renal syndrome (HFRS), caused by Old World hantaviruses in Europe and Asia, and hantavirus cardiopulmonary syndrome (HCPS), also called hantavirus pulmonary syndrome (HPS), caused by New World hantaviruses in the Americas.[3] Although HFRS and HCPS are recognized as distinct clinical entities, there are overlapping symptoms, signs, and pathogenic alterations.[3] Nephropathia epidemica (NE) is a mild form of HFRS caused primarily by Puumala virus.[3]

Classification

Taxonomic Classification

Hantaviruses belong to the family Hantaviridae, which was reclassified in 2024 under the order Elliovirales, class Bunyaviricetes (previously order Bunyavirales).[2] The family encompasses seven genera and 53 species.[4] All human-pathogenic hantaviruses belong to the genus Orthohantavirus within the subfamily Mammantavirinae.[1] The family also includes subfamilies Actantavirinae (reptile-associated) and Repantavirinae (fish-associated), as well as genera Loanvirus, Mobatvirus, and Thottimvirus (associated with shrews, moles, and bats).[1][5]

Hantavirids produce enveloped virions (80–120 nm in diameter) containing three single-stranded RNA segments with open reading frames that encode a nucleoprotein (N), a glycoprotein precursor (GPC), and a large (L) protein containing an RNA-dependent RNA polymerase (RdRP) domain.[1] The total genome size is approximately 10.5–14.6 kb.[1]

Taxonomic Rank Classification
Realm Riboviria
Kingdom Orthornavirae
Phylum Negarnaviricota
Subphylum Polyploviricotina
Class Bunyaviricetes
Order Elliovirales
Family Hantaviridae
Subfamily (human-pathogenic) Mammantavirinae
Genus (human-pathogenic) Orthohantavirus

Adapted from ICTV Virus Taxonomy Profile: Hantaviridae 2024.[1][2]

Clinical Syndrome-Based Classification

Hantavirus infection can be classified on the basis of the clinical manifestations and the type of hantavirus responsible for the manifestation. The clinical manifestations include hantavirus cardiopulmonary syndrome (HCPS), hemorrhagic fever with renal syndrome (HFRS), and nephropathia epidemica (NE).[3][6]

HFRS is endemic in Europe and Asia and is characterized by increased vascular permeability, coagulopathy, and acute kidney injury. The disease course may include five phases: febrile, hypotensive, oliguric, diuretic, and convalescent.[3] HFRS-causing hantaviruses include Hantaan virus (HTNV), Dobrava-Belgrade virus (DOBV), Puumala virus (PUUV), Seoul virus (SEOV), and Tula virus (TULV, rare).[3]

HCPS (also called hantavirus pulmonary syndrome, HPS) is endemic in the Americas and is characterized by respiratory failure and cardiogenic shock. HCPS begins with a febrile prodrome followed by a cardiopulmonary phase with sudden onset of cough, dyspnea, tachycardia, and hypotension, leading to non-cardiogenic pulmonary edema.[3] The most common causes are Sin Nombre virus (SNV) in North America and Andes virus (ANDV) in South America.[3]

NE (nephropathia epidemica) is a mild form of HFRS caused primarily by Puumala virus (PUUV), with a case fatality rate (CFR) 1%.[3][7]

Although HFRS and HCPS are recognized as distinct clinical entities, there are overlapping symptoms, signs, and pathogenic alterations. Both syndromes can lead to renal failure, and virtually all patients with HCPS and more than half of patients with HFRS have respiratory symptoms.[3]

Classification by Virus, Host, Geography, and Clinical Syndrome

Hantavirus Cardiopulmonary Syndrome (HCPS)

The most severe forms of HCPS are associated with Sin Nombre virus, Andes virus, Araraquara virus, and Juquitiba virus, all with CFRs between 30% and 45%. Choclo virus (CHOV) and Laguna Negra virus (LANV) have a CFR between 12% and 15%.[3] Andes virus is unique among hantaviruses for documented person-to-person transmission.[3]

Virus Abbreviation Primary Rodent Host Geographic Distribution CFR
Sin Nombre virus SNV Peromyscus maniculatus (deer mouse) North America (western USA, Canada) ~36%
Andes virus ANDV Oligoryzomys longicaudatus (long-tailed colilargo) Argentina, Chile 30–45%
Araraquara virus ARAV Necromys lasiurus Brazil 30–45%
Juquitiba virus JUQV Oligoryzomys nigripes Brazil, Argentina 30–45%
New York virus NYV Peromyscus leucopus (white-footed mouse) North America (eastern USA)
Monongahela virus MGLV Peromyscus leucopus North America (eastern USA)
Bayou virus BAYV Oryzomys palustris (marsh rice rat) North America (southeastern USA)
Black Creek Canal virus BCCV Sigmodon hispidus (hispid cotton rat) North America (southeastern USA)
Muleshoe virus MULEV Sigmodon hispidus North America
Choclo virus CHOV Oligoryzomys fulvescens Panama 12–15%
Laguna Negra virus LANV Calomys callosus Argentina, Paraguay, Bolivia 12–15%
Bermejo virus BMJV Oligoryzomys chacoensis, O. flavescens Bolivia, Argentina
Lechiguanas virus LECV Oligoryzomys flavescens Argentina
Oran virus ORNV Oligoryzomys chacoensis Argentina
Maciel virus MCLV Bolomys obscurus Argentina
Castelo Dos Sonhos virus CASV Oligoryzomys spp. Brazil

Adapted from Vial et al. 2023,[3] Jiang et al. 2017,[6] and Avšič-Županc et al. 2019.[7] CFR = case fatality rate. "—" indicates insufficient data for reliable CFR estimate.

Hemorrhagic Fever with Renal Syndrome (HFRS)

HFRS caused by Hantaan virus, Amur virus, and Dobrava-Belgrade virus (genotype Dobrava) are more severe, with mortality rates from 5% to 15%, whereas Seoul virus causes moderate disease and Puumala virus and Saaremaa virus (DOBV-Aa genotype) cause mild forms of disease with mortality rates 1%.[7][3]

Virus Abbreviation Primary Rodent Host Geographic Distribution Severity / CFR
Hantaan virus HTNV Apodemus agrarius (striped field mouse) China, Russia, Korea Severe; CFR 5–15% (historically), ~1–1.3% (modern)
Amur virus AMRV Apodemus peninsulae (Korean field mouse) China, Russia, Korea Severe; CFR 5–15%
Dobrava-Belgrade virus (genotype Dobrava, DOBV-Af) DOBV Apodemus flavicollis (yellow-necked mouse) Balkans, southeastern Europe Severe; CFR 10–12%
Dobrava-Belgrade virus (genotype Sochi, DOBV-Ap) DOBV-Ap Apodemus ponticus (Black Sea field mouse) Southern Russia (Sochi district) Moderate to severe
Dobrava-Belgrade virus (genotype Kurkino) DOBV-Kurkino Apodemus agrarius (striped field mouse) Central Europe (Germany, Poland, Lithuania) Mild; no lethal outcomes reported
Saaremaa virus (DOBV-Aa genotype) SAAV Apodemus agrarius (striped field mouse) Estonia, Russia, Finland, Germany, Denmark, Slovenia Mild; CFR 1%
Seoul virus SEOV Rattus norvegicus (brown rat), R. rattus Global (via international shipping) Moderate; CFR 1–2%
Puumala virus PUUV Myodes glareolus (bank vole) Northern/western Europe, Russia Mild (NE); CFR 1% (0.1–0.4%)
Thailand hantavirus THAIV Bandicota indica Thailand Rare; limited data
Tula virus TULV Microtus arvalis (common vole) Europe Very rare; only a few human cases reported

Adapted from Vial et al. 2023,[3] Avšič-Županc et al. 2019,[7] Vaheri et al. 2013,[8] and Klempa et al. 2013.[9] CFR = case fatality rate.

Nephropathia Epidemica (NE)

Nephropathia epidemica is a mild form of HFRS caused primarily by Puumala virus (PUUV) and, to a lesser extent, by the Saaremaa virus (DOBV-Aa genotype). NE is the most common hantavirus disease in Europe, with Finland reporting the highest number of cases.[3][8]

Virus Primary Rodent Host Geographic Distribution
Puumala virus (PUUV) Myodes glareolus (bank vole) Northern/western Europe, Russia, Finland
Saaremaa virus (DOBV-Aa) Apodemus agrarius (striped field mouse) Estonia, central/eastern Europe

Dobrava-Belgrade Virus Genotype Classification

Dobrava-Belgrade virus (DOBV) has a complex taxonomy with four recognized genotypes that differ in phylogeny, host reservoir, geographic distribution, and pathogenicity for humans:[9][8]

Genotype Alternate Name Rodent Host Geography Pathogenicity
Dobrava (DOBV-Af) DOBV Apodemus flavicollis (yellow-necked mouse) Balkans, southeastern Europe Severe; CFR up to 12%
Kurkino DOBV-Kurkino Apodemus agrarius (striped field mouse) Central Europe (Germany, Poland, Lithuania, Czech Republic) Mild; no lethal outcomes reported
Sochi (DOBV-Ap) DOBV-Ap Apodemus ponticus (Black Sea field mouse) Southern Russia (Sochi district) Moderate to severe
Saaremaa (DOBV-Aa) SAAV Apodemus agrarius (striped field mouse) Estonia, Russia, Finland, Germany, Denmark, Slovenia, Croatia, Slovakia Mild

Adapted from Klempa et al. 2013[9] and Vaheri et al. 2013.[8]

Phylogenetic Host-Based Classification

A newer framework proposes classifying orthohantaviruses into three phylogenetically based rodent host groups rather than the traditional Old World versus New World geographic dichotomy. This framework better accounts for the fact that related arvicoline rodents and their orthohantaviruses are found in both hemispheres, making the geographic dichotomy imprecise.[10]

Host Group Rodent Subfamily/Family Representative Viruses Geography Primary Syndrome
Murinae-associated Family Muridae HTNV, SEOV, DOBV Asia, Europe HFRS
Arvicolinae-associated Subfamily of Cricetidae PUUV, Tula virus Europe, parts of North America Mild HFRS / NE
Sigmodontinae/Neotominae-associated Subfamily of Cricetidae SNV, ANDV, and other New World viruses Americas HCPS

Adapted from Mull et al. 2023.[10]

There are currently 58 distinct orthohantaviruses recognized, with over 24 recognized as pathogenic to humans. Case fatality of pathogenic orthohantaviruses ranges from 0.1% to 50%.[10]

Epidemiological Summary

Approximately 200,000 cases of hantavirus infection are reported worldwide per year.[10] Key epidemiological data by region include:

China: A mean of 12,800 HFRS cases per year (2004–2016), with a CFR of 1.3%.[3]

European Union: A mean of 3,100 HFRS cases per year; Finland reports 43%, Germany 30%, and Sweden 6% of all cases. CFR ranges from 0.03% (Germany) to 0.4% (Sweden) for PUUV, and 10–12% for DOBV in the Balkans.[3]

Russia: Approximately 7,300 HFRS cases per year, with an overall CFR of 0.4%.[3]

Americas: Approximately 300 HCPS cases per year, mainly in Argentina, Brazil, and Chile.[3]

South Korea: 300–600 cases per year; CFR has decreased from 5–7% (1950s) to 1% (2011–2016).[3]

From serosurveillance studies in Finland, only around 15% of infected people are diagnosed and reported.[3] The incubation period ranges from 2 to 6 weeks.[3] Transmission mainly occurs via inhalation of aerosolized rodent excreta (urine, feces, saliva). Person-to-person transmission has been documented only for Andes virus.[3]

References

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Bradfute SB, Calisher CH, Klempa B, Klingström J, Kuhn JH, Laenen L, Maes P, Papa A, Schmaljohn CS, Tischler ND, Plyusnin A (2024). "ICTV Virus Taxonomy Profile: Hantaviridae 2024". J Gen Virol. 105 (4). doi:10.1099/jgv.0.001975. PMID 38587456 Check |pmid= value (help).
  2. 2.0 2.1 2.2 Kuhn JH, Brown K, Adkins S, de la Torre JC, Digiaro M, Ergünay K, Forber PJ, Goldbach RW, Grybchuk D, Hughes HR, Junglen S, Klempa B, Krupovic M, Lambert AJ, Maes P, Marklewitz M, Mielke-Ehret N, Mirazimi A, Mühlbach HP, Palacios G, Pawęska JT, Peters CJ, Plyusnin A, Rubbenstroth D, Shi M, Siddell SG, Simmonds P, Sironi M, Smagghe G, Tesh RB, Turina M, Wahl V, Walker PJ, Wang L, Whitfield AE, Yeh SD, Zerbini FM, Zhang YZ (2024). "Promotion of Order Bunyavirales to Class Bunyaviricetes to Accommodate a Rapidly Increasing Number of Related Polyploviricotine Viruses". J Virol. 98 (10): e0106924. doi:10.1128/jvi.01069-24. PMID 39303014 Check |pmid= value (help).
  3. 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3.20 3.21 3.22 3.23 Vial PA, Ferrés M, Vial C, Klingström J, Ahlm C, López R, Le Corre N, Mertz GJ (2023). "Hantavirus in Humans: A Review of Clinical Aspects and Management". Lancet Infect Dis. 23 (9): e371–e382. doi:10.1016/S1473-3099(23)00128-7. PMID 37105214 Check |pmid= value (help).
  4. Chen RX, Gong HY, Wang X, Zhang L, Bao DL (2023). "Zoonotic Hantaviridae With Global Public Health Significance". Viruses. 15 (8): 1705. doi:10.3390/v15081705. PMID 37632047 Check |pmid= value (help).
  5. Laenen L, Vergote V, Calisher CH, Klempa B, Klingström J, Kuhn JH, Maes P (2019). "Hantaviridae: Current Classification and Future Perspectives". Viruses. 11 (9): E788. doi:10.3390/v11090788. PMID 31461937.
  6. 6.0 6.1 Jiang H, Zheng X, Wang L, Du H, Wang P, Bai X (2017). "Hantavirus infection: a global zoonotic challenge". Virol Sin. 32 (1): 32–43. doi:10.1007/s12250-016-3899-x. PMID 28120221.
  7. 7.0 7.1 7.2 7.3 Avšič-Županc T, Saksida A, Korva M (2019). "Hantavirus Infections". Clin Microbiol Infect. 21S: e6–e16. doi:10.1111/1469-0691.12291. PMID 24750436.
  8. 8.0 8.1 8.2 8.3 Vaheri A, Henttonen H, Voutilainen L, Mustonen J, Sironen T, Vapalahti O (2013). "Hantavirus infections in Europe and their impact on public health". Rev Med Virol. 23 (1): 35–49. doi:10.1002/rmv.1722. PMID 23280975.
  9. 9.0 9.1 9.2 Klempa B, Avsic-Zupanc T, Clement J, Dzagurova TK, Henttonen H, Heyman P, Jakab F, Kruger DH, Maes P, Papa A, Tkachenko EA, Ulrich RG, Vapalahti O, Vaheri A (2013). "Complex Evolution and Epidemiology of Dobrava-Belgrade Hantavirus: Definition of Genotypes and Their Characteristics". Arch Virol. 158 (3): 521–9. doi:10.1007/s00705-012-1514-5. PMID 23090188.
  10. 10.0 10.1 10.2 10.3 Mull N, Seifert SN, Forbes KM (2023). "A Framework for Understanding and Predicting Orthohantavirus Functional Traits". Trends Microbiol. 31 (11): 1102–1110. doi:10.1016/j.tim.2023.05.004. PMID 37277284 Check |pmid= value (help).

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