Candidiasis laboratory tests

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Candidiasis Main page

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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]

Overview

Candidiasis, commonly called yeast infection or thrush, is a fungal infection (mycosis) of any of the Candida species, of which Candida albicans is the most common.[1][2] Candidiasis thereby encompasses infections that range from superficial, such as oral thrush and vaginitis, to systemic and potentially life-threatening diseases. Medical professionals use two primary methods to diagnose yeast infections: microscopic examination, and culturing.

For the microscope method, a scraping or swab of the affected area is placed on a microscope slide. A single drop of 10% potassium hydroxide (KOH) solution is then also placed on the slide. The KOH dissolves the skin cells but leaves the Candida untouched, so that when the slide is viewed under a microscope, the hyphae and pseudo spores of Candida are visible. Their presence in large numbers strongly suggests a yeast infection.

For the culturing method, a sterile swab is rubbed on the infected skin surface. The swab is then rubbed across a culture medium. The medium is incubated for several days, during which time colonies of yeast and/or bacteria develop. The characteristics of the colonies provide a presumptive diagnosis of the organism causing symptoms.

References

  1. Walsh TJ, Dixon DM (1996). "Deep Mycoses". In Baron S et al eds. Baron's Medical Microbiology (via NCBI Bookshelf) (4th ed. ed.). Univ of Texas Medical Branch. ISBN 0-9631172-1-1.
  2. MedlinePlus Encyclopedia Vaginal yeast infection

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