Botulism/Lab Tests
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Overview
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Seyedmahdi Pahlavani, M.D. [2]
Overview
Toxin assay is the prefered method of laboratory work up for diagnosis botulism. Serum, stool, gastric secretions, and suspected food sources should be collected and toxin assay must be performed. However, role of clinical suspicion must not be undertaken.[1][2][3][4][5][6]
Laboratory Findings
Toxin assay is the prefered method of laboratory work up for diagnosis botulism. Serum, stool, gastric secretions, and suspected food sources should be collected and toxin assay must be performed. Urine toxicology screen must be performed to rule out other possible diseases, such as substance abuse, medications, and environmental exposure.[1][2][3][4][5][6]
- In order to diagnose wound botulism, wound exudates and swab sampling must be done in addition to toxin assay. Wound culture should be performed in anaerobic media.
- Confirmatory diagnosis of infant botulism is based on serum and stool screening for botulism's toxins or isolation of toxigenic C botulinum in stool.
- Cerebrospinal fluid analysis should be performed to rule out other diseases mimicking botulism. However, minimal protein elevation is a common finding in botulism.
- Laboratory testing may take hours or days. Initial diagnosis and appropriate treatment depend on clinical diagnosis through a thorough history and physical examination.
- The most traditional way for laboratory diagnosis is, injecting the serum sample of suspected person to mouse and following mice up for symptom development. To detect the toxin type, the affected mice must be injected by type-specific anti toxin. Botulism symptoms are absent in mice that received the appropriate anti toxin.
- Novel assays use mass spectroscopy instead of mouse bioassay.
- Toxin excretion and positive stool culture may be remain for one month after infection.
- ELISA and PCR of suspected food source for toxin assay are another helpful method of diagnosis.
The following gallery shows microscopic features of C. botulinum.
Gallery
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![Clostridium botulinum growing on egg yolk agar showing the lipase reaction 72hrs. From Public Health Image Library (PHIL). [7]](/images/d/d5/Botulism20.jpeg)
Clostridium botulinum growing on egg yolk agar showing the lipase reaction 72hrs. From Public Health Image Library (PHIL). [7]
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![Clostridium botulinum spores stained with malachite green stain. From Public Health Image Library (PHIL). [7]](/images/6/6b/Botulism19.jpeg)
Clostridium botulinum spores stained with malachite green stain. From Public Health Image Library (PHIL). [7]
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![Clostridium botulinum spores stained with malachite green stain. From Public Health Image Library (PHIL). [7]](/images/d/d2/Botulism18.jpeg)
Clostridium botulinum spores stained with malachite green stain. From Public Health Image Library (PHIL). [7]
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![Clostridium botulinum Type A colonies blood agar plate 24hrs. From Public Health Image Library (PHIL). [7]](/images/3/35/Botulism12.jpeg)
Clostridium botulinum Type A colonies blood agar plate 24hrs. From Public Health Image Library (PHIL). [7]
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![Clostridium botulinum Type A colonies blood agar plate 72hrs (5x). From Public Health Image Library (PHIL). [7]](/images/f/f7/Botulism11.jpeg)
Clostridium botulinum Type A colonies blood agar plate 72hrs (5x). From Public Health Image Library (PHIL). [7]
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![Clostridium botulinum Type-A in thioglycollate broth was incubated for 48hrs. From Public Health Image Library (PHIL). [7]](/images/c/c7/Botulism10.jpeg)
Clostridium botulinum Type-A in thioglycollate broth was incubated for 48hrs. From Public Health Image Library (PHIL). [7]
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![Clostridium botulinum Type E colonies displaying an opaque zone grown on a 48hr egg yolk agar plate (1.9x mag). From Public Health Image Library (PHIL). [7]](/images/4/48/Botulism09.jpeg)
Clostridium botulinum Type E colonies displaying an opaque zone grown on a 48hr egg yolk agar plate (1.9x mag). From Public Health Image Library (PHIL). [7]
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![Contaminated Jalapeño peppers involved in an outbreak of botulism. From Public Health Image Library (PHIL). [7]](/images/9/9c/Botulism08.jpeg)
Contaminated Jalapeño peppers involved in an outbreak of botulism. From Public Health Image Library (PHIL). [7]
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![Clostridium sp. Gram-positive bacteria, which had been grown on a 4% blood agar plate (BAP) 48hrs. From Public Health Image Library (PHIL). [7]](/images/1/10/Botulism07.jpeg)
Clostridium sp. Gram-positive bacteria, which had been grown on a 4% blood agar plate (BAP) 48hrs. From Public Health Image Library (PHIL). [7]
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![Clostridium innocuum bacteria cultivated in a thioglycollate fluid medium 24hrs (956x mag). From Public Health Image Library (PHIL). [7]](/images/4/43/Botulism05.jpeg)
Clostridium innocuum bacteria cultivated in a thioglycollate fluid medium 24hrs (956x mag). From Public Health Image Library (PHIL). [7]
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![Gram-positive Clostridium subterminale bacteria on BAP medium 48hrs (956x mag). From Public Health Image Library (PHIL). [7]](/images/3/3f/Botulism04.jpeg)
Gram-positive Clostridium subterminale bacteria on BAP medium 48hrs (956x mag). From Public Health Image Library (PHIL). [7]
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![Clostridium sp. Gram-positive bacteria grown on a chopped meat medium 48hrs (956x mag). From Public Health Image Library (PHIL). [7]](/images/7/7c/Botulism03.jpeg)
Clostridium sp. Gram-positive bacteria grown on a chopped meat medium 48hrs (956x mag). From Public Health Image Library (PHIL). [7]
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![Clostridium sp. Gram-positive bacteria grown on a chopped meat medium 48hrs (956x mag). From Public Health Image Library (PHIL). [7]](/images/f/f9/Botulism01.jpeg)
Clostridium sp. Gram-positive bacteria grown on a chopped meat medium 48hrs (956x mag). From Public Health Image Library (PHIL). [7]
![Clostridium botulinum growing on egg yolk agar showing the lipase reaction 72hrs. From Public Health Image Library (PHIL). [7]](/index.php/File:Botulism20.jpeg)
![Clostridium botulinum spores stained with malachite green stain. From Public Health Image Library (PHIL). [7]](/index.php/File:Botulism19.jpeg)
![Clostridium botulinum spores stained with malachite green stain. From Public Health Image Library (PHIL). [7]](/index.php/File:Botulism18.jpeg)
![Clostridium botulinum Type A colonies blood agar plate 24hrs. From Public Health Image Library (PHIL). [7]](/index.php/File:Botulism12.jpeg)
![Clostridium botulinum Type A colonies blood agar plate 72hrs (5x). From Public Health Image Library (PHIL). [7]](/index.php/File:Botulism11.jpeg)
![Clostridium botulinum Type-A in thioglycollate broth was incubated for 48hrs. From Public Health Image Library (PHIL). [7]](/index.php/File:Botulism10.jpeg)
![Clostridium botulinum Type E colonies displaying an opaque zone grown on a 48hr egg yolk agar plate (1.9x mag). From Public Health Image Library (PHIL). [7]](/index.php/File:Botulism09.jpeg)
![Contaminated Jalapeño peppers involved in an outbreak of botulism. From Public Health Image Library (PHIL). [7]](/index.php/File:Botulism08.jpeg)
![Clostridium sp. Gram-positive bacteria, which had been grown on a 4% blood agar plate (BAP) 48hrs. From Public Health Image Library (PHIL). [7]](/index.php/File:Botulism07.jpeg)
![Clostridium innocuum bacteria cultivated in a thioglycollate fluid medium 24hrs (956x mag). From Public Health Image Library (PHIL). [7]](/index.php/File:Botulism05.jpeg)
![Gram-positive Clostridium subterminale bacteria on BAP medium 48hrs (956x mag). From Public Health Image Library (PHIL). [7]](/index.php/File:Botulism04.jpeg)
![Clostridium sp. Gram-positive bacteria grown on a chopped meat medium 48hrs (956x mag). From Public Health Image Library (PHIL). [7]](/index.php/File:Botulism03.jpeg)
![Clostridium sp. Gram-positive bacteria grown on a chopped meat medium 48hrs (956x mag). From Public Health Image Library (PHIL). [7]](/index.php/File:Botulism01.jpeg)
References
- ↑ 1.0 1.1 Sharma SK, Ferreira JL, Eblen BS, Whiting RC (2006). "Detection of type A, B, E, and F Clostridium botulinum neurotoxins in foods by using an amplified enzyme-linked immunosorbent assay with digoxigenin-labeled antibodies". Appl. Environ. Microbiol. 72 (2): 1231–8. doi:10.1128/AEM.72.2.1231-1238.2006. PMC 1392902. PMID 16461671.
- ↑ 2.0 2.1 Dowell VR, McCroskey LM, Hatheway CL, Lombard GL, Hughes JM, Merson MH (1977). "Coproexamination for botulinal toxin and clostridium botulinum. A new procedure for laboratory diagnosis of botulism". JAMA. 238 (17): 1829–32. PMID 333132.
- ↑ 3.0 3.1 Zhang Y, Lou J, Jenko KL, Marks JD, Varnum SM (2012). "Simultaneous and sensitive detection of six serotypes of botulinum neurotoxin using enzyme-linked immunosorbent assay-based protein antibody microarrays". Anal. Biochem. 430 (2): 185–92. doi:10.1016/j.ab.2012.08.021. PMC 3589981. PMID 22935296.
- ↑ 4.0 4.1 Lindström M, Korkeala H (2006). "Laboratory diagnostics of botulism". Clin. Microbiol. Rev. 19 (2): 298–314. doi:10.1128/CMR.19.2.298-314.2006. PMC 1471988. PMID 16614251.
- ↑ 5.0 5.1 Satterfield BA, Stewart AF, Lew CS, Pickett DO, Cohen MN, Moore EA, Luedtke PF, O'Neill KL, Robison RA (2010). "A quadruplex real-time PCR assay for rapid detection and differentiation of the Clostridium botulinum toxin genes A, B, E and F". J. Med. Microbiol. 59 (Pt 1): 55–64. doi:10.1099/jmm.0.012567-0. PMID 19779029.
- ↑ 6.0 6.1 Mazuet C, Ezan E, Volland H, Popoff MR, Becher F (2012). "Toxin detection in patients' sera by mass spectrometry during two outbreaks of type A Botulism in France". J. Clin. Microbiol. 50 (12): 4091–4. doi:10.1128/JCM.02392-12. PMC 3502950. PMID 22993181.
- ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 "Public Health Image Library (PHIL)".
Related Pages
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- Used To Diagnose:Used To Diagnose::Botulism
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