2-hydroxymuconate-semialdehyde hydrolase

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In enzymology, a 2-hydroxymuconate-semialdehyde hydrolase (EC is an enzyme that catalyzes the chemical reaction

2-hydroxymuconate semialdehyde + H2O formate + 2-oxopent-4-enoate

Thus, the two substrates of this enzyme are 2-hydroxymuconate semialdehyde and H2O, whereas its two products are formate and 2-oxopent-4-enoate.

This enzyme belongs to the family of hydrolases, specifically those acting on carbon-carbon bonds in ketonic substances. The systematic name of this enzyme class is 2-hydroxymuconate-semialdehyde formylhydrolase. Other names in common use include 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase, 2-hydroxymuconic semialdehyde hydrolase, HMSH, and HOD hydrolase. This enzyme participates in 5 metabolic pathways: benzoate degradation via hydroxylation, toluene and xylene degradation, 1,4-dichlorobenzene degradation, carbazole degradation, and styrene degradation.

Structural studies

As of late 2007, 10 structures have been solved for this class of enzymes, with PDB accession codes 1IUN, 1IUO, 1IUP, 1UK6, 1UK7, 1UK8, 1UK9, 1UKA, 1UKB, and 2D0D.


  • IUBMB entry for
  • BRENDA references for (Recommended.)
  • PubMed references for
  • PubMed Central references for
  • Google Scholar references for
  • Harayama S, Rekik M, Wasserfallen A and Bairoch A (1987). "Evolutionary relationships between catabolic pathways for aromatics: conservtion of gene order and nucleotide sequences of catechol oxidation genes of pWW0 and NAH7 plasmids". MGG Mol. Gen. Genet. 210: 241&ndash, 247.
  • Sala-Trepat JM, Evans WC (1971). "The meta cleavage of catechol by Azotobacter species 4-Oxalocrotonate pathway". Eur. J. Biochem. 20: 400&ndash, 13. PMID 4325686.

External links

The CAS registry number for this enzyme class is 54004-61-4.

Gene Ontology (GO) codes