mRNA display is a display technique used to perform in vitro protein, and/or peptide evolution to create molecules that can bind to a desired ligand. The process results in translated peptides or proteins that are associated with their mRNA progenitor, via a puromycin linkage, which is used, as a complex, to bind to an immobilized ligand in a selection step (affinity chromatography). The mRNA-protein fusions that bind well are then reverse transcribed to cDNA and their sequence amplified via a polymerase chain reaction. The end result is a nucleotide sequence that encodes peptides which tightly bind molecules of interest.
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