Counterimmunoelectrophoresis
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Overview
A laboratory technique used to evaluate the binding of an antibody to its antigen. Counterimmunoelectrophoresis is similar to immunodiffusion, but with the addition of an applied electrical field across the diffusion medium, usually an agar or polyacrylamide gel. The effect is rapid migration of the antibody and antigen out of their respective wells towards one another to form a line of precipitation, or a precipitin line, indicating binding.
References
- Sherris, John C.; Ryan, Kenneth J.; Ray, C. L. (2004). Sherris medical microbiology: an introduction to infectious diseases. New York: McGraw-Hill, Chp 15 Principles of Laboratory Diagnosis. ISBN 0-8385-8529-9.
External links
Immunologic techniques and tests - diagnostic immunology | |
|---|---|
| Immunoprecipitation | Chromatin immunoprecipitation - Immunodiffusion (Ouchterlony double immuno diffusion, Radial immunodiffusion, Immunoelectrophoresis, Counterimmunoelectrophoresis) |
| Immunoassay | ELISA - Enzyme Multiplied Immunoassay Technique - RAST test - Radioimmunoassay - Immunofluorescence |
| Other | Nephelometry - Agglutination (Hemagglutination) - Complement fixation test - Immunohistochemistry |
Acknowledgement and Attribution Regarding Sources of Content
Some of the initial content on this page may be incorporated in part from copyleft sources in the public domain including wikis such as Wikipedia and AskDrWiki. Drug information for patients came from the The National Library of Medicine. Infectious disease information may have come from the Centers for Disease Control (CDC). Differential Diagnoses are drawn from clinicians as well as an amalgamation of 3 sources: 1.The Disease Database; 2. Kahan, Scott, Smith, Ellen G. In A Page: Signs and Symptoms. Malden, Massachusetts: Blackwell Publishing, 2004:3; 3. Sailer, Christian, Wasner, Susanne. Differential Diagnosis Pocket. Hermosa Beach, CA: Borm Bruckmeir Publishing LLC, 2002:7 .
