View source for SUPT5H

{{Infobox_gene}}
'''Transcription elongation factor SPT5''' is a [[protein]] that in humans is encoded by the ''SUPT5H'' [[gene]].<ref name="pmid8975720">{{cite journal | vauthors = Chiang PW, Fogel E, Jackson CL, Lieuallen K, Lennon G, Qu X, Wang SQ, Kurnit DM | title = Isolation, sequencing, and mapping of the human homologue of the yeast transcription factor, SPT5 | journal = Genomics | volume = 38 | issue = 3 | pages = 421–4 | date = Dec 1996 | pmid = 8975720 | pmc =  | doi = 10.1006/geno.1996.0646 }}</ref><ref>{{cite web | title = Entrez Gene: SUPT5H Suppressor of Ty 5 homolog (S. cerevisiae)| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=6829| accessdate = }}</ref>

== Interactions ==

SUPT5H has been shown to [[Protein-protein interaction|interact]] with:
{{div col|colwidth=20em}}
* [[CDK9]],<ref name = pmid10958691/> 
* [[Cyclin-dependent kinase 7]],<ref name = pmid10958691>{{cite journal | vauthors = Garber ME, Mayall TP, Suess EM, Meisenhelder J, Thompson NE, Jones KA | title = CDK9 autophosphorylation regulates high-affinity binding of the human immunodeficiency virus type 1 tat-P-TEFb complex to TAR RNA | journal = Molecular and Cellular Biology | volume = 20 | issue = 18 | pages = 6958–69 | date = Sep 2000 | pmid = 10958691 | pmc = 88771 | doi =  10.1128/mcb.20.18.6958-6969.2000}}</ref> 
* [[HTATSF1]],<ref name = pmid10454543>{{cite journal | vauthors = Kim JB, Yamaguchi Y, Wada T, Handa H, Sharp PA | title = Tat-SF1 protein associates with RAP30 and human SPT5 proteins | journal = Molecular and Cellular Biology | volume = 19 | issue = 9 | pages = 5960–8 | date = Sep 1999 | pmid = 10454543 | pmc = 84462 | doi =  10.1128/mcb.19.9.5960}}</ref> 
* [[PIN1]],<ref name = pmid11575923>{{cite journal | vauthors = Lavoie SB, Albert AL, Handa H, Vincent M, Bensaude O | title = The peptidyl-prolyl isomerase Pin1 interacts with hSpt5 phosphorylated by Cdk9 | journal = Journal of Molecular Biology | volume = 312 | issue = 4 | pages = 675–85 | date = Sep 2001 | pmid = 11575923 | doi = 10.1006/jmbi.2001.4991 }}</ref> 
* [[POLR2A]],<ref name = pmid10454543/><ref name = pmid9450929>{{cite journal | vauthors = Wada T, Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog GA, Winston F, Buratowski S, Handa H | title = DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs | journal = Genes & Development | volume = 12 | issue = 3 | pages = 343–56 | date = Feb 1998 | pmid = 9450929 | pmc = 316480 | doi =  10.1101/gad.12.3.343}}</ref> 
* [[PRMT1]]<ref name = pmid12718890>{{cite journal | vauthors = Kwak YT, Guo J, Prajapati S, Park KJ, Surabhi RM, Miller B, Gehrig P, Gaynor RB | title = Methylation of SPT5 regulates its interaction with RNA polymerase II and transcriptional elongation properties | journal = Molecular Cell | volume = 11 | issue = 4 | pages = 1055–66 | date = Apr 2003 | pmid = 12718890 | doi =  10.1016/s1097-2765(03)00101-1}}</ref>  and
* [[Protein arginine methyltransferase 5]].<ref name = pmid12718890/>
{{Div col end}}

==Model organisms==
[[Model organism]]s have been used in the study of SUPT5H function. A conditional [[knockout mouse]] line called ''Supt5<sup>tm2a(KOMP)Wtsi</sup>'' was generated at the [[Wellcome Trust Sanger Institute]].<ref name="mgp_reference">{{cite journal |title=The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice |author=Gerdin AK |year=2010 |journal=Acta Ophthalmologica|volume=88 |pages=925–7|doi=10.1111/j.1755-3768.2010.4142.x }}</ref> Male and female animals underwent a standardized [[phenotypic screen]]<ref name="IMPCsearch_ref">{{cite web |url=http://www.mousephenotype.org/data/search?q=Supt5#fq=*:*&facet=gene |title=International Mouse Phenotyping Consortium}}</ref> to determine the effects of deletion.<ref name="pmid21677750">{{cite journal | vauthors = Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A | title = A conditional knockout resource for the genome-wide study of mouse gene function | journal = Nature | volume = 474 | issue = 7351 | pages = 337–42 | date = Jun 2011 | pmid = 21677750 | pmc = 3572410 | doi = 10.1038/nature10163 }}</ref><ref name="mouse_library">{{cite journal | vauthors = Dolgin E | title = Mouse library set to be knockout | journal = Nature | volume = 474 | issue = 7351 | pages = 262–3 | date = Jun 2011 | pmid = 21677718 | doi = 10.1038/474262a }}</ref><ref name="mouse_for_all_reasons">{{cite journal | vauthors = Collins FS, Rossant J, Wurst W | title = A mouse for all reasons | journal = Cell | volume = 128 | issue = 1 | pages = 9–13 | date = Jan 2007 | pmid = 17218247 | doi = 10.1016/j.cell.2006.12.018 }}</ref><ref name="pmid23870131">{{cite journal | vauthors = White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP | title = Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes | journal = Cell | volume = 154 | issue = 2 | pages = 452–64 | date = Jul 2013 | pmid = 23870131 | doi = 10.1016/j.cell.2013.06.022 | pmc=3717207}}</ref> Additional screens performed:  - In-depth immunological phenotyping<ref name="iii_ref">{{cite web |url= http://www.immunophenotyping.org/data/search?keys=Supt5&field_gene_construct_tid=All |title=Infection and Immunity Immunophenotyping (3i) Consortium}}</ref> 
{| class="wikitable sortable collapsible collapsed" border="1" cellpadding="2" style="float: left;" |
|+ ''Supt5'' knockout mouse phenotype
|-
! Characteristic!! Phenotype
|-
| colspan=2; style="text-align: center;" | All data available at.<ref name="IMPCsearch_ref"/><ref name="iii_ref" />

|-
| Peripheral blood leukocytes 6 Weeks || bgcolor="#488ED3"|Normal

|-
| ''[[Haematology]]'' 6 Weeks || bgcolor="#488ED3"|Normal

|-
| Homozygous viability at P14 || bgcolor="#C40000"|Abnormal

|-
| [[Recessive]] lethal study || bgcolor="#C40000"|Abnormal

|-
| Body weight || bgcolor="#488ED3"|Normal

|-
| Neurological assessment || bgcolor="#488ED3"|Normal

|-
| Grip strength || bgcolor="#488ED3"|Normal

|-
| [[Dysmorphology]] || bgcolor="#488ED3"|Normal

|-
| [[Indirect calorimetry]] || bgcolor="#488ED3"|Normal

|-
| [[Glucose tolerance test]] || bgcolor="#488ED3"|Normal

|-
| [[Auditory brainstem response]] || bgcolor="#488ED3"|Normal

|-
| [[Dual-energy X-ray absorptiometry|DEXA]] || bgcolor="#488ED3"|Normal

|-
| [[Radiography]] || bgcolor="#488ED3"|Normal

|-
| Eye morphology || bgcolor="#488ED3"|Normal

|-
| [[Clinical chemistry]] || bgcolor="#488ED3"|Normal

|-
| ''[[Haematology]]'' 16 Weeks || bgcolor="#488ED3"|Normal

|-
| Peripheral blood leukocytes 16 Weeks || bgcolor="#488ED3"|Normal

|-
| Heart weight || bgcolor="#488ED3"|Normal

|-
| ''[[Salmonella]]'' infection || bgcolor="#488ED3"|Normal

|-
| Cytotoxic T Cell Function || bgcolor="#488ED3"|Normal

|-
| Spleen Immunophenotyping || bgcolor="#488ED3"|Normal

|-
| Mesenteric Lymph Node Immunophenotyping || bgcolor="#488ED3"|Normal

|-
| Bone Marrow Immunophenotyping || bgcolor="#488ED3"|Normal

|-
| Epidermal Immune Composition || bgcolor="#488ED3"|Normal

|-
|}
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== References ==
{{reflist}}

== Further reading ==
{{Refbegin | 2}}
* {{cite journal | vauthors = Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA | title = A "double adaptor" method for improved shotgun library construction | journal = Analytical Biochemistry | volume = 236 | issue = 1 | pages = 107–13 | date = Apr 1996 | pmid = 8619474 | doi = 10.1006/abio.1996.0138 }}
* {{cite journal | vauthors = Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA | title = Large-scale concatenation cDNA sequencing | journal = Genome Research | volume = 7 | issue = 4 | pages = 353–8 | date = Apr 1997 | pmid = 9110174 | pmc = 139146 | doi = 10.1101/gr.7.4.353 }}
* {{cite journal | vauthors = Stachora AA, Schäfer RE, Pohlmeier M, Maier G, Ponstingl H | title = Human Supt5h protein, a putative modulator of chromatin structure, is reversibly phosphorylated in mitosis | journal = FEBS Letters | volume = 409 | issue = 1 | pages = 74–8 | date = Jun 1997 | pmid = 9199507 | doi = 10.1016/S0014-5793(97)00486-9 }}
* {{cite journal | vauthors = Wada T, Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog GA, Winston F, Buratowski S, Handa H | title = DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs | journal = Genes & Development | volume = 12 | issue = 3 | pages = 343–56 | date = Feb 1998 | pmid = 9450929 | pmc = 316480 | doi = 10.1101/gad.12.3.343 }}
* {{cite journal | vauthors = Wu-Baer F, Lane WS, Gaynor RB | title = Role of the human homolog of the yeast transcription factor SPT5 in HIV-1 Tat-activation | journal = Journal of Molecular Biology | volume = 277 | issue = 2 | pages = 179–97 | date = Mar 1998 | pmid = 9514752 | doi = 10.1006/jmbi.1997.1601 }}
* {{cite journal | vauthors = Wada T, Takagi T, Yamaguchi Y, Watanabe D, Handa H | title = Evidence that P-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro | journal = The EMBO Journal | volume = 17 | issue = 24 | pages = 7395–403 | date = Dec 1998 | pmid = 9857195 | pmc = 1171084 | doi = 10.1093/emboj/17.24.7395 }}
* {{cite journal | vauthors = Yamaguchi Y, Wada T, Watanabe D, Takagi T, Hasegawa J, Handa H | title = Structure and function of the human transcription elongation factor DSIF | journal = The Journal of Biological Chemistry | volume = 274 | issue = 12 | pages = 8085–92 | date = Mar 1999 | pmid = 10075709 | doi = 10.1074/jbc.274.12.8085 }}
* {{cite journal | vauthors = Yamaguchi Y, Takagi T, Wada T, Yano K, Furuya A, Sugimoto S, Hasegawa J, Handa H | title = NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation | journal = Cell | volume = 97 | issue = 1 | pages = 41–51 | date = Apr 1999 | pmid = 10199401 | doi = 10.1016/S0092-8674(00)80713-8 }}
* {{cite journal | vauthors = Parada CA, Roeder RG | title = A novel RNA polymerase II-containing complex potentiates Tat-enhanced HIV-1 transcription | journal = The EMBO Journal | volume = 18 | issue = 13 | pages = 3688–701 | date = Jul 1999 | pmid = 10393184 | pmc = 1171446 | doi = 10.1093/emboj/18.13.3688 }}
* {{cite journal | vauthors = Wen Y, Shatkin AJ | title = Transcription elongation factor hSPT5 stimulates mRNA capping | journal = Genes & Development | volume = 13 | issue = 14 | pages = 1774–9 | date = Jul 1999 | pmid = 10421630 | pmc = 316881 | doi = 10.1101/gad.13.14.1774 }}
* {{cite journal | vauthors = Kim JB, Yamaguchi Y, Wada T, Handa H, Sharp PA | title = Tat-SF1 protein associates with RAP30 and human SPT5 proteins | journal = Molecular and Cellular Biology | volume = 19 | issue = 9 | pages = 5960–8 | date = Sep 1999 | pmid = 10454543 | pmc = 84462 | doi =  10.1128/mcb.19.9.5960}}
* {{cite journal | vauthors = Ivanov D, Kwak YT, Guo J, Gaynor RB | title = Domains in the SPT5 protein that modulate its transcriptional regulatory properties | journal = Molecular and Cellular Biology | volume = 20 | issue = 9 | pages = 2970–83 | date = May 2000 | pmid = 10757782 | pmc = 85557 | doi = 10.1128/MCB.20.9.2970-2983.2000 }}
* {{cite journal | vauthors = Wada T, Orphanides G, Hasegawa J, Kim DK, Shima D, Yamaguchi Y, Fukuda A, Hisatake K, Oh S, Reinberg D, Handa H | title = FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between P-TEFb and TFIIH | journal = Molecular Cell | volume = 5 | issue = 6 | pages = 1067–72 | date = Jun 2000 | pmid = 10912001 | doi = 10.1016/S1097-2765(00)80272-5 }}
* {{cite journal | vauthors = Ping YH, Rana TM | title = DSIF and NELF interact with RNA polymerase II elongation complex and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA polymerase II and DSIF during transcription elongation | journal = The Journal of Biological Chemistry | volume = 276 | issue = 16 | pages = 12951–8 | date = Apr 2001 | pmid = 11112772 | doi = 10.1074/jbc.M006130200 }}
* {{cite journal | vauthors = Kim JB, Sharp PA | title = Positive transcription elongation factor B phosphorylates hSPT5 and RNA polymerase II carboxyl-terminal domain independently of cyclin-dependent kinase-activating kinase | journal = The Journal of Biological Chemistry | volume = 276 | issue = 15 | pages = 12317–23 | date = Apr 2001 | pmid = 11145967 | doi = 10.1074/jbc.M010908200 }}
* {{cite journal | vauthors = Renner DB, Yamaguchi Y, Wada T, Handa H, Price DH | title = A highly purified RNA polymerase II elongation control system | journal = The Journal of Biological Chemistry | volume = 276 | issue = 45 | pages = 42601–9 | date = Nov 2001 | pmid = 11553615 | doi = 10.1074/jbc.M104967200 }}
* {{cite journal | vauthors = Lavoie SB, Albert AL, Handa H, Vincent M, Bensaude O | title = The peptidyl-prolyl isomerase Pin1 interacts with hSpt5 phosphorylated by Cdk9 | journal = Journal of Molecular Biology | volume = 312 | issue = 4 | pages = 675–85 | date = Sep 2001 | pmid = 11575923 | doi = 10.1006/jmbi.2001.4991 }}
* {{cite journal | vauthors = Bourgeois CF, Kim YK, Churcher MJ, West MJ, Karn J | title = Spt5 cooperates with human immunodeficiency virus type 1 Tat by preventing premature RNA release at terminator sequences | journal = Molecular and Cellular Biology | volume = 22 | issue = 4 | pages = 1079–93 | date = Feb 2002 | pmid = 11809800 | pmc = 134635 | doi = 10.1128/MCB.22.4.1079-1093.2002 }}
* {{cite journal | vauthors = Yamaguchi Y, Inukai N, Narita T, Wada T, Handa H | title = Evidence that negative elongation factor represses transcription elongation through binding to a DRB sensitivity-inducing factor/RNA polymerase II complex and RNA | journal = Molecular and Cellular Biology | volume = 22 | issue = 9 | pages = 2918–27 | date = May 2002 | pmid = 11940650 | pmc = 133766 | doi = 10.1128/MCB.22.9.2918-2927.2002 }}
{{Refend}}

{{PDB Gallery|geneid=6829}}