PPP1R8

Protein phosphatase 1, regulatory (inhibitor) subunit 8
File:PBB Protein PPP1R8 image.jpg PDB rendering based on 2jpe.
Available structures PDB Ortholog search: Template:Homologene2PDBe PDBe, Template:Homologene2uniprot RCSB List of PDB id codes 2jpe​
Identifiers
Symbols	PPP1R8 ; ARD1; ARD-1; NIPP-1; NIPP1; PRO2047
External IDs	Template:OMIM5 Template:MGI HomoloGene: 8555
Gene ontology Molecular function Template:GNF GO Template:GNF GO Template:GNF GO Template:GNF GO Template:GNF GO Template:GNF GO Template:GNF GO Template:GNF GO Template:GNF GO Cellular component Template:GNF GO Template:GNF GO Biological process Template:GNF GO Template:GNF GO Template:GNF GO Template:GNF GO Template:GNF GO Template:GNF GO
RNA expression pattern
File:PBB GE PPP1R8 207830 s at tn.png
More reference expression data
Orthologs
Template:GNF Ortholog box
Species	Human	Mouse
Entrez	n/a	n/a
Ensembl	n/a	n/a
UniProt	n/a	n/a
RefSeq (mRNA)	n/a	n/a
RefSeq (protein)	n/a	n/a
Location (UCSC)	n/a	n/a
PubMed search	n/a	n/a

Protein phosphatase 1, regulatory (inhibitor) subunit 8, also known as PPP1R8, is a human gene.

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.

Further reading

• Van Eynde A, Wera S, Beullens M; et al. (1996). "Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing". J. Biol. Chem. 270 (47): 28068–74. PMID 7499293.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Wang M, Cohen SN (1994). "ard-1: a human gene that reverses the effects of temperature-sensitive and deletion mutations in the Escherichia coli rne gene and encodes an activity producing RNase E-like cleavages". Proc. Natl. Acad. Sci. U.S.A. 91 (22): 10591–5. PMID 7524097.
• Mishima K, Tsuchiya M, Nightingale MS; et al. (1993). "ARD 1, a 64-kDa guanine nucleotide-binding protein with a carboxyl-terminal ADP-ribosylation factor domain". J. Biol. Chem. 268 (12): 8801–7. PMID 8473324.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Claverie-Martin F, Wang M, Cohen SN (1997). "ARD-1 cDNA from human cells encodes a site-specific single-strand endoribonuclease that functionally resembles Escherichia coli RNase E.". J. Biol. Chem. 272 (21): 13823–8. PMID 9153239.CS1 maint: Multiple names: authors list (link)
• Vulsteke V, Beullens M, Waelkens E; et al. (1998). "Properties and phosphorylation sites of baculovirus-expressed nuclear inhibitor of protein phosphatase-1 (NIPP-1)". J. Biol. Chem. 272 (52): 32972–8. PMID 9407077.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Van Eynde A, Pérez-Callejón E, Schoenmakers E; et al. (1999). "Organization and alternate splice products of the gene encoding nuclear inhibitor of protein phosphatase-1 (NIPP-1)". Eur. J. Biochem. 261 (1): 291–300. PMID 10103062.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Jin Q, Beullens M, Jagiello I; et al. (1999). "Mapping of the RNA-binding and endoribonuclease domains of NIPP1, a nuclear targeting subunit of protein phosphatase 1". Biochem. J. 342 ( Pt 1): 13–9. PMID 10432294.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Chang AC, Sohlberg B, Trinkle-Mulcahy L; et al. (2000). "Alternative splicing regulates the production of ARD-1 endoribonuclease and NIPP-1, an inhibitor of protein phosphatase-1, as isoforms encoded by the same gene". Gene. 240 (1): 45–55. PMID 10564811.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Boudrez A, Beullens M, Groenen P; et al. (2000). "NIPP1-mediated interaction of protein phosphatase-1 with CDC5L, a regulator of pre-mRNA splicing and mitotic entry". J. Biol. Chem. 275 (33): 25411–7. doi:10.1074/jbc.M001676200. PMID 10827081.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Jagiello I, Van Eynde A, Vulsteke V; et al. (2001). "Nuclear and subnuclear targeting sequences of the protein phosphatase-1 regulator NIPP1". J. Cell. Sci. 113 Pt 21: 3761–8. PMID 11034904.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Beullens M, Vulsteke V, Van Eynde A; et al. (2001). "The C-terminus of NIPP1 (nuclear inhibitor of protein phosphatase-1) contains a novel binding site for protein phosphatase-1 that is controlled by tyrosine phosphorylation and RNA binding". Biochem. J. 352 Pt 3: 651–8. PMID 11104670.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Trinkle-Mulcahy L, Sleeman JE, Lamond AI (2002). "Dynamic targeting of protein phosphatase 1 within the nuclei of living mammalian cells". J. Cell. Sci. 114 (Pt 23): 4219–28. PMID 11739654.CS1 maint: Multiple names: authors list (link)
• Beullens M, Bollen M (2002). "The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly". J. Biol. Chem. 277 (22): 19855–60. doi:10.1074/jbc.M200847200. PMID 11909864.
• Boudrez A, Beullens M, Waelkens E; et al. (2002). "Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1". J. Biol. Chem. 277 (35): 31834–41. doi:10.1074/jbc.M204427200. PMID 12105215.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Strausberg RL, Feingold EA, Grouse LH; et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Ammosova T, Jerebtsova M, Beullens M; et al. (2003). "Nuclear protein phosphatase-1 regulates HIV-1 transcription". J. Biol. Chem. 278 (34): 32189–94. doi:10.1074/jbc.M300521200. PMID 12788939.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Jin Q, van Eynde A, Beullens M; et al. (2003). "The protein phosphatase-1 (PP1) regulator, nuclear inhibitor of PP1 (NIPP1), interacts with the polycomb group protein, embryonic ectoderm development (EED), and functions as a transcriptional repressor". J. Biol. Chem. 278 (33): 30677–85. doi:10.1074/jbc.M302273200. PMID 12788942.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Vulsteke V, Beullens M, Boudrez A; et al. (2004). "Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1". J. Biol. Chem. 279 (10): 8642–7. doi:10.1074/jbc.M311466200. PMID 14699119.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Colland F, Jacq X, Trouplin V; et al. (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMID 15231748.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)
• Gerhard DS, Wagner L, Feingold EA; et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334.CS1 maint: Explicit use of et al. (link) CS1 maint: Multiple names: authors list (link)