View source for POLR3G

{{Infobox_gene}}

'''Polymerase (RNA) III (DNA directed) polypeptide G (32kD)''' is a [[protein]] that in humans is encoded by the POLR3G [[gene]].
<ref name="entrez">
{{cite web
| title = Entrez Gene: Polymerase (RNA) III (DNA directed) polypeptide G (32kD)
| url = https://www.ncbi.nlm.nih.gov/gene/10622
| accessdate = 2014-04-01 <!-- T09:30:46.781513-08:00 --> 
}}</ref>

==Model organisms==
[[Model organism]]s have been used in the study of POLR3G function. A conditional [[knockout mouse]] line called ''Polr3g<sup>tm1a(EUCOMM)Wtsi</sup>'' was generated at the [[Wellcome Trust Sanger Institute]].<ref name="mgp_reference">{{cite journal |title=The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice |author=Gerdin AK |year=2010 |journal=Acta Ophthalmologica|volume=88 |pages=925–7|doi=10.1111/j.1755-3768.2010.4142.x }}</ref> Male and female animals underwent a standardized [[phenotypic screen]]<ref name="IMPCsearch_ref">{{cite web |url=http://www.mousephenotype.org/data/search?q=Polr3g#fq=*:*&facet=gene |title=International Mouse Phenotyping Consortium}}</ref> to determine the effects of deletion.<ref name="pmid21677750">{{cite journal | vauthors = Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A | title = A conditional knockout resource for the genome-wide study of mouse gene function | journal = Nature | volume = 474 | issue = 7351 | pages = 337–42 | date = Jun 2011 | pmid = 21677750 | pmc = 3572410 | doi = 10.1038/nature10163 }}</ref><ref name="mouse_library">{{cite journal | vauthors = Dolgin E | title = Mouse library set to be knockout | journal = Nature | volume = 474 | issue = 7351 | pages = 262–3 | date = Jun 2011 | pmid = 21677718 | doi = 10.1038/474262a }}</ref><ref name="mouse_for_all_reasons">{{cite journal | vauthors = Collins FS, Rossant J, Wurst W | title = A mouse for all reasons | journal = Cell | volume = 128 | issue = 1 | pages = 9–13 | date = Jan 2007 | pmid = 17218247 | doi = 10.1016/j.cell.2006.12.018 }}</ref><ref name="pmid23870131">{{cite journal | vauthors = White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, ((Sanger Institute Mouse Genetics Project)), Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP | title = Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes | journal = Cell | volume = 154 | issue = 2 | pages = 452–64 | year = 2013 | pmid = 23870131 | doi = 10.1016/j.cell.2013.06.022 | pmc=3717207}}</ref> Additional screens performed:  - In-depth immunological phenotyping<ref name="iii_ref">{{cite web |url= http://www.immunophenotyping.org/data/search?keys=Polr3g&field_gene_construct_tid=All |title=Infection and Immunity Immunophenotyping (3i) Consortium}}</ref> 
{| class="wikitable sortable collapsible collapsed" border="1" cellpadding="2" style="float: left;" |
|+ ''Polr3g'' knockout mouse phenotype
|-
! Characteristic!! Phenotype
|-
| colspan=2; style="text-align: center;" | All data available at.<ref name="IMPCsearch_ref"/><ref name="iii_ref" />

|-
| Insulin || bgcolor="#488ED3"|Normal

|-
| Homozygous viability at P14 || bgcolor="#C40000"|Abnormal

|-
| [[Recessive]] lethal study || bgcolor="#C40000"|Abnormal

|-
| Body weight || bgcolor="#488ED3"|Normal

|-
| Neurological assessment || bgcolor="#488ED3"|Normal

|-
| Grip strength || bgcolor="#488ED3"|Normal

|-
| [[Dysmorphology]] || bgcolor="#488ED3"|Normal

|-
| [[Indirect calorimetry]] || bgcolor="#488ED3"|Normal

|-
| [[Glucose tolerance test]] || bgcolor="#488ED3"|Normal

|-
| [[Auditory brainstem response]] || bgcolor="#488ED3"|Normal

|-
| [[Dual-energy X-ray absorptiometry|DEXA]] || bgcolor="#488ED3"|Normal

|-
| [[Radiography]] || bgcolor="#488ED3"|Normal

|-
| Eye morphology || bgcolor="#488ED3"|Normal

|-
| [[Clinical chemistry]] || bgcolor="#488ED3"|Normal

|-
| ''[[Haematology]]'' 16 Weeks || bgcolor="#488ED3"|Normal

|-
| Peripheral blood leukocytes 16 Weeks || bgcolor="#488ED3"|Normal

|-
| Heart weight || bgcolor="#488ED3"|Normal

|-
| Cytotoxic T Cell Function || bgcolor="#488ED3"|Normal

|-
| Spleen Immunophenotyping || bgcolor="#488ED3"|Normal

|-
| Mesenteric Lymph Node Immunophenotyping || bgcolor="#488ED3"|Normal

|-
| Bone Marrow Immunophenotyping || bgcolor="#488ED3"|Normal

|-
| Epidermal Immune Composition || bgcolor="#488ED3"|Normal

|-
| Influenza Challenge || bgcolor="#488ED3"|Normal

|-
|}''<br />
<br />''

==References==
{{reflist}}

== Further reading ==

{{refbegin | 2}}
*{{Cite journal
 | pmid = 19609254
| year = 2009
| author1 = Ablasser
| first1 = A
| title = RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate
| journal = Nature Immunology
| volume = 10
| issue = 10
| pages = 1065–72
| last2 = Bauernfeind
| first2 = F
| last3 = Hartmann
| first3 = G
| last4 = Latz
| first4 = E
| last5 = Fitzgerald
| first5 = K. A.
| last6 = Hornung
| first6 = V
| doi = 10.1038/ni.1779
| pmc = 3878616
| url = https://infoscience.epfl.ch/record/198826/files/nihms527360.pdf
}}
*{{Cite journal
 | pmid = 19631370
| year = 2009
| author1 = Chiu
| first1 = Y. H.
| title = RNA polymerase III detects cytosolic DNA and induces type I interferons through the RIG-I pathway
| journal = Cell
| volume = 138
| issue = 3
| pages = 576–91
| last2 = MacMillan
| first2 = J. B.
| last3 = Chen
| first3 = Z. J.
| doi = 10.1016/j.cell.2009.06.015
| pmc = 2747301
}}
*{{Cite journal
 | pmid = 21810746
| year = 2011
| author1 = Chantarangsu
| first1 = S
| title = Genome-wide association study identifies variations in 6p21.3 associated with nevirapine-induced rash
| journal = Clinical Infectious Diseases
| volume = 53
| issue = 4
| pages = 341–8
| last2 = Mushiroda
| first2 = T
| last3 = Mahasirimongkol
| first3 = S
| last4 = Kiertiburanakul
| first4 = S
| last5 = Sungkanuparph
| first5 = S
| last6 = Manosuthi
| first6 = W
| last7 = Tantisiriwat
| first7 = W
| last8 = Charoenyingwattana
| first8 = A
| last9 = Sura
| first9 = T
| last10 = Takahashi
| first10 = A
| last11 = Kubo
| first11 = M
| last12 = Kamatani
| first12 = N
| last13 = Chantratita
| first13 = W
| last14 = Nakamura
| first14 = Y
| doi = 10.1093/cid/cir403
}}
*{{Cite journal
 | pmid = 21898682
| year = 2011
| author1 = Wong
| first1 = R. C.
| title = A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells
| journal = Stem Cells
| volume = 29
| issue = 10
| pages = 1517–27
| last2 = Pollan
| first2 = S
| last3 = Fong
| first3 = H
| last4 = Ibrahim
| first4 = A
| last5 = Smith
| first5 = E. L.
| last6 = Ho
| first6 = M
| last7 = Laslett
| first7 = A. L.
| last8 = Donovan
| first8 = P. J.
| doi = 10.1002/stem.714
}}
*{{Cite journal
 | pmid = 1403646
| year = 1992
| author1 = Jang
| first1 = K. L.
| title = The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC
| journal = Journal of Acquired Immune Deficiency Syndromes
| volume = 5
| issue = 11
| pages = 1142–7
| last2 = Collins
| first2 = M. K.
| last3 = Latchman
| first3 = D. S.
}}
*{{Cite journal
 | pmid = 9171375
| year = 1997
| author1 = Wang
| first1 = Z
| title = Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation
| journal = Genes & Development
| volume = 11
| issue = 10
| pages = 1315–26
| last2 = Roeder
| first2 = R. G.
 | doi=10.1101/gad.11.10.1315
}}
*{{Cite journal
 | pmid = 12391170
| year = 2002
| author1 = Hu
| first1 = P
| title = Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits
| journal = Molecular and Cellular Biology
| volume = 22
| issue = 22
| pages = 8044–55
| last2 = Wu
| first2 = S
| last3 = Sun
| first3 = Y
| last4 = Yuan
| first4 = C. C.
| last5 = Kobayashi
| first5 = R
| last6 = Myers
| first6 = M. P.
| last7 = Hernandez
| first7 = N
| pmc = 134740
 | doi=10.1128/MCB.22.22.8044-8055.2002
}}

{{refend}}

[[Category:Human proteins]]


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