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'''Lectins''' are carbohydrate-binding [[proteins]] or [[glycoproteins]] which are highly specific for their sugar [[moieties]].  
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{{SI}}
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'''Lectins''' are sugar-binding [[proteins]] which are highly specific for their sugar [[moieties]]. They typically play a role in biological recognition phenomena involving cells and proteins. For example, some bacteria use lectins to attach themselves to the cells of the host organism during infection.
 +
 
[[Image:Hemagglutinin_lateral.jpg|thumb|lateral hemagglutinine]]
 
[[Image:Hemagglutinin_lateral.jpg|thumb|lateral hemagglutinine]]
 +
 
==Etymology==
 
==Etymology==
 
The name ‘lectin’ is derived from the Latin word ''legere'', meaning ‘to select’.
 
The name ‘lectin’ is derived from the Latin word ''legere'', meaning ‘to select’.
  
 
==History==
 
==History==
Although they were first discovered more than 100 years ago in plants, they are now known to be present throughout nature.  
+
Although they were first discovered more than 100 years ago in plants, they are now known to be present throughout nature.
  
 
It is generally believed that the earliest description of such a [[hemagglutinin]] was by [[Peter Hermann Stillmark]] in his doctoral thesis presented in 1888 to the University of [[Dorpat]], (one of the oldest universities in czarist [[Russia]]). This [[hemagglutinin]], which was also highly toxic, was isolated by Stillmark from seeds of the [[Ricinus|castor tree (Ricinus communis)]] and was named [[ricin]].
 
It is generally believed that the earliest description of such a [[hemagglutinin]] was by [[Peter Hermann Stillmark]] in his doctoral thesis presented in 1888 to the University of [[Dorpat]], (one of the oldest universities in czarist [[Russia]]). This [[hemagglutinin]], which was also highly toxic, was isolated by Stillmark from seeds of the [[Ricinus|castor tree (Ricinus communis)]] and was named [[ricin]].
  
 
==Biological functions==
 
==Biological functions==
Most of the lectins are basically non-enzymic in action and non-immune in origin. Lectins occur ubiquitously in nature. They may bind carbohydrate moiety as such free in solution or carbohydrate moiety which is a part of protein/particulate body. They agglutinate cells and/or precipitates glycoconjugates.
+
Most of the lectins are basically non-enzymic in action and non-immune in origin. Lectins occur ubiquitously in nature. They may bind to a soluble carbohydrate or to a carbohydrate moiety which is a part of a glycoprotein or glycolipid. They typically agglutinate certain animal cells and/or precipitate glycoconjugates.
  
 
  [[Image:gs4 sugar all.png|thumb|An oligosaccharide (shown in grey) bound in the binding site of a plant lectin (''Griffonia simplicifolia'' isolectin IV in complex with the Lewis b blood group determinant). Only a part of the oligosaccharide (central, in grey) is shown for clarity.]]
 
  [[Image:gs4 sugar all.png|thumb|An oligosaccharide (shown in grey) bound in the binding site of a plant lectin (''Griffonia simplicifolia'' isolectin IV in complex with the Lewis b blood group determinant). Only a part of the oligosaccharide (central, in grey) is shown for clarity.]]
  
 
===Function in animals===
 
===Function in animals===
While the function of lectins in plants is believed to be the binding of [[glycoprotein]]s on the surface of cells, their role in animals also includes the binding of soluble extracellular and intercellular glycoproteins.  
+
While the function of lectins in plants is believed to be the binding of [[glycoprotein]]s on the surface of parasitic cells, their role in animals also includes the binding of soluble extracellular and intercellular glycoproteins.  
  
 
For example, there are lectins found on the surface of mammalian liver cells that specifically recognize [[galactose]] residues. It is believed that these cell-surface receptors are responsible for the removal of certain glycoproteins from the circulatory system.  
 
For example, there are lectins found on the surface of mammalian liver cells that specifically recognize [[galactose]] residues. It is believed that these cell-surface receptors are responsible for the removal of certain glycoproteins from the circulatory system.  
  
Another example is the [[mannose-6-phosphate]] receptor that recognizes hydrolytic enzymes containing this residue and subsequently targets these proteins for delivery to the [[lysosome]]s. (one defect in this particular system is know as [[I-cell disease]].)
+
Another example is the [[mannose-6-phosphate]] receptor that recognizes hydrolytic enzymes containing this residue and subsequently targets these proteins for delivery to the [[lysosome]]s. (one defect in this particular system is known as [[I-cell disease]].)
 
 
They serve many different biological functions from the regulation of [[cell adhesion]] to glycoprotein synthesis and the control of protein levels in the [[blood]].
 
  
Lectins are also known to play important roles in the [[immune system]] by recognising carbohydrates that are found exclusively on [[pathogen]]s, or that are inaccessible on host cells. Examples are the lectin [[mannan-binding lectin pathway|complement activation pathway]] and [[Mannose binding lectin]].
+
Lectins serve many different biological functions from the regulation of [[cell adhesion]] to glycoprotein synthesis and the control of protein levels in the [[blood]].  
  
 +
Lectins are also known to play important roles in the [[immune system]] by recognizing carbohydrates that are found exclusively on [[pathogen]]s, or that are inaccessible on host cells. Examples are the lectin [[mannan-binding lectin pathway|complement activation pathway]] and [[Mannose binding lectin]].
  
 
===Function in plants===
 
===Function in plants===
The real function of lectins in plants is still to be found - they are not necessary for [[rhizobia]] binding as mentioned above (ruled out with lectin-knockout [[transgene]]) and cell adhesion function as their sole purpose in plants also questionable.  
+
The function of lectins in plants is still uncertain.  Once thought to be necessary for [[rhizobia]] binding, this proposed function was ruled out through lectin-knockout [[transgene]] studies.
  
Large presence in seeds (from which lectins are usually isolated) decreases with growth is suggesting a great role in plant's [[germination]] and perhaps in the seed's survival itself.
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The large concentration of lectins in plant seeds decreases with growth, and suggests a role in plant [[germination]] and perhaps in the seed's survival itself.
  
==Use in medicine and technology==
+
==Use in science, medicine and technology==
  
===Clinical use in blood typing===
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===Use in medicine and medical research===
 
Purified lectins are important in a clinical setting because they are used for [[blood typing]]. Some of the glycolipids and glycoproteins on an individual's red blood cells can be identified by lectins.  
 
Purified lectins are important in a clinical setting because they are used for [[blood typing]]. Some of the glycolipids and glycoproteins on an individual's red blood cells can be identified by lectins.  
 
* A lectin from ''[[Dolichos]] biflorus'' is used to identify cells that belong to the A1 blood group.  
 
* A lectin from ''[[Dolichos]] biflorus'' is used to identify cells that belong to the A1 blood group.  
 
* A lectin from ''[[Ulex]] europaeus'' is used to identify the H blood group antigen.
 
* A lectin from ''[[Ulex]] europaeus'' is used to identify the H blood group antigen.
 
* A lectin from ''[[Vicia]] graminea'' is used to identify the N blood group antigen.
 
* A lectin from ''[[Vicia]] graminea'' is used to identify the N blood group antigen.
 +
[[PHA-L]], a lectin from the [[kidney bean]], is used by neuroscientists to trace the path of [[efferent]] [[axon]]s. This usage is called the [[anterograde labeling method]].<ref name="Carlson">{{cite book
 +
| first= Neil R.
 +
| last= Carlson
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| year= 2007
 +
| month=
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| title= Physiology of Behavior, 9th ed.
 +
| pages= 144
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| location= Boston
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| publisher=Pearson Education, Inc.
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| id= ISBN 0-205-46724-5
 +
}}</ref>
  
 
===Use in studying carbohydrate recognition by proteins===
 
===Use in studying carbohydrate recognition by proteins===
Lectins from legume plants have been widely used as model systems to understand the molecular basis of how proteins recognize carbohydrates, because they are relatively easy to obtain and have a wide variety of sugar specificities. The many [[crystal structures]] of legume lectins have led to a detailed insight of the atomic interactions between carbohydrates and proteins.
+
Lectins from legume plants, such as [[PHA]] or [[concanavalin A]], have been widely used as model systems to understand the molecular basis of how proteins recognize carbohydrates, because they are relatively easy to obtain and have a wide variety of sugar specificities. The many [[crystal structures]] of legume lectins have led to a detailed insight of the atomic interactions between carbohydrates and proteins.
  
 
===Use in biochemical warfare===
 
===Use in biochemical warfare===
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* One is a lectin that binds cell surface galactosyl residues and enables the protein to enter cells.  
 
* One is a lectin that binds cell surface galactosyl residues and enables the protein to enter cells.  
 
* The second domain is an N-[[glycosidase]] that cleaves nucleobases from ribosomal RNA resulting in inhibition of protein synthesis and cell death.
 
* The second domain is an N-[[glycosidase]] that cleaves nucleobases from ribosomal RNA resulting in inhibition of protein synthesis and cell death.
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 +
  
 
==See also==
 
==See also==
Line 52: Line 69:
  
 
==References==
 
==References==
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<references/>
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* {{cite journal |author=Loris R, Hamelryck T, Bouckaert J, Wyns L |title=Legume lectin structure |journal=Biochim. Biophys. Acta |volume=1383 |issue=1 |pages=9-36 |year=1998 |pmid=9546043 |url=http://www.binf.ku.dk/~thamelry/publications/bba_review.pdf}}
 
* {{cite journal |author=Loris R, Hamelryck T, Bouckaert J, Wyns L |title=Legume lectin structure |journal=Biochim. Biophys. Acta |volume=1383 |issue=1 |pages=9-36 |year=1998 |pmid=9546043 |url=http://www.binf.ku.dk/~thamelry/publications/bba_review.pdf}}
  
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[[Category:Glycoproteins]]
 
[[Category:Glycoproteins]]
  
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[[cs:Lektin]]
 
[[de:Lektin]]
 
[[de:Lektin]]
 
[[fr:Lectine]]
 
[[fr:Lectine]]
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[[he:לקטין]]
 
[[nl:Lectine]]
 
[[nl:Lectine]]
 
[[ja:レクチン]]
 
[[ja:レクチン]]
 
[[no:Lektiner]]
 
[[no:Lektiner]]
 
[[pt:Lectina]]
 
[[pt:Lectina]]
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[[ru:Лектины]]
 
[[zh:凝集素]]
 
[[zh:凝集素]]
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{{WH}}
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{{WS}}
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{{jb1}}

Latest revision as of 16:49, 9 August 2012

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Lectins are sugar-binding proteins which are highly specific for their sugar moieties. They typically play a role in biological recognition phenomena involving cells and proteins. For example, some bacteria use lectins to attach themselves to the cells of the host organism during infection.

lateral hemagglutinine

Etymology

The name ‘lectin’ is derived from the Latin word legere, meaning ‘to select’.

History

Although they were first discovered more than 100 years ago in plants, they are now known to be present throughout nature.

It is generally believed that the earliest description of such a hemagglutinin was by Peter Hermann Stillmark in his doctoral thesis presented in 1888 to the University of Dorpat, (one of the oldest universities in czarist Russia). This hemagglutinin, which was also highly toxic, was isolated by Stillmark from seeds of the castor tree (Ricinus communis) and was named ricin.

Biological functions

Most of the lectins are basically non-enzymic in action and non-immune in origin. Lectins occur ubiquitously in nature. They may bind to a soluble carbohydrate or to a carbohydrate moiety which is a part of a glycoprotein or glycolipid. They typically agglutinate certain animal cells and/or precipitate glycoconjugates.

An oligosaccharide (shown in grey) bound in the binding site of a plant lectin (Griffonia simplicifolia isolectin IV in complex with the Lewis b blood group determinant). Only a part of the oligosaccharide (central, in grey) is shown for clarity.

Function in animals

While the function of lectins in plants is believed to be the binding of glycoproteins on the surface of parasitic cells, their role in animals also includes the binding of soluble extracellular and intercellular glycoproteins.

For example, there are lectins found on the surface of mammalian liver cells that specifically recognize galactose residues. It is believed that these cell-surface receptors are responsible for the removal of certain glycoproteins from the circulatory system.

Another example is the mannose-6-phosphate receptor that recognizes hydrolytic enzymes containing this residue and subsequently targets these proteins for delivery to the lysosomes. (one defect in this particular system is known as I-cell disease.)

Lectins serve many different biological functions from the regulation of cell adhesion to glycoprotein synthesis and the control of protein levels in the blood.

Lectins are also known to play important roles in the immune system by recognizing carbohydrates that are found exclusively on pathogens, or that are inaccessible on host cells. Examples are the lectin complement activation pathway and Mannose binding lectin.

Function in plants

The function of lectins in plants is still uncertain. Once thought to be necessary for rhizobia binding, this proposed function was ruled out through lectin-knockout transgene studies.

The large concentration of lectins in plant seeds decreases with growth, and suggests a role in plant germination and perhaps in the seed's survival itself.

Use in science, medicine and technology

Use in medicine and medical research

Purified lectins are important in a clinical setting because they are used for blood typing. Some of the glycolipids and glycoproteins on an individual's red blood cells can be identified by lectins.

  • A lectin from Dolichos biflorus is used to identify cells that belong to the A1 blood group.
  • A lectin from Ulex europaeus is used to identify the H blood group antigen.
  • A lectin from Vicia graminea is used to identify the N blood group antigen.

PHA-L, a lectin from the kidney bean, is used by neuroscientists to trace the path of efferent axons. This usage is called the anterograde labeling method.[1]

Use in studying carbohydrate recognition by proteins

Lectins from legume plants, such as PHA or concanavalin A, have been widely used as model systems to understand the molecular basis of how proteins recognize carbohydrates, because they are relatively easy to obtain and have a wide variety of sugar specificities. The many crystal structures of legume lectins have led to a detailed insight of the atomic interactions between carbohydrates and proteins.

Use in biochemical warfare

One example of the powerful biological attributes of lectins is the biochemical warfare agent ricin. Ricin is isolated from seeds of the castor oil plant and is a protein that comprises two domains,

  • One is a lectin that binds cell surface galactosyl residues and enables the protein to enter cells.
  • The second domain is an N-glycosidase that cleaves nucleobases from ribosomal RNA resulting in inhibition of protein synthesis and cell death.


See also

References

  1. Carlson, Neil R. (2007). Physiology of Behavior, 9th ed. Boston: Pearson Education, Inc. p. 144. ISBN 0-205-46724-5.
  • Sharon, N., Lis, H. Lectins, Second Edition (2003) Kluwer Academic

External links

  • World of Lectin links maintained by Thorkild C. Bøg-Hansen
  • Ni Y, Tizard I (1996). "Lectin-carbohydrate interaction in the immune system". Vet Immunol Immunopathol. 55 (1–3): 205–23. PMID 9014318.
  • EY Laboratories, Inc World's largest lectin manufacturer.

cs:Lektin de:Lektin he:לקטין nl:Lectine no:Lektiner


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