Helicobacter pylori infection diagnostic tests: Difference between revisions
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===Rapid Urease Testing (RUT)=== | ===Rapid Urease Testing (RUT)=== | ||
*Identifies active H.pylori infection through the organism's urease activity. | *Identifies active H.pylori infection through the organism's urease activity. | ||
*''' | *'''Procedure''' | ||
:*The gastric biopsies obtained are placed into an agar gel or on a reaction strip containing urea, a buffer,and a pH-sensitive indicator. | :*The gastric biopsies obtained are placed into an agar gel or on a reaction strip containing urea, a buffer,and a pH-sensitive indicator. | ||
:*The urea is metabolized to ammonia and bicarbonate in the presence of H.pylori's urease leading to a pH increase in the microenvironment of the organism. | :*The urea is metabolized to ammonia and bicarbonate in the presence of H.pylori's urease leading to a pH increase in the microenvironment of the organism. |
Revision as of 13:10, 17 January 2017
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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1] Associate Editor(s)-in-Chief: Yamuna Kondapally, M.B.B.S[2]
Overview
Endoscopic Diagnostic Tests
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Endoscopic diagnostic tests are biopsy-based diagnostic methods for H.pylori infection. These include:
- Histology
- Rapid urease testing
- Culture
- Polymerase chain reaction
Histology
- Histology for H.pylori detection depends on:
- Site, number, and size of gastric biopsies
- Method of staining
- Level of experience of the examining pathologist
- Advantage of histology over other diagnostic studies is its ability to detect the pathological changes associated with H.pylori infection such as inflammation, atrophy, intestinal metaplasia and malignancy.
- Multiple biopsies are required for accurate diagnosis as the prevalence and density of H.pylori varies throughout the stomach. Therefore a minimum of three biopsies are taken from different sites. They are:
- Angularis
- Greater curvature of the corpus
- Greater curvature of the antrum
- The sensitivity of histology is greatly effected by the use of medications such as bismuth, antibiotics, and PPI.
Rapid Urease Testing (RUT)
- Identifies active H.pylori infection through the organism's urease activity.
- Procedure
- The gastric biopsies obtained are placed into an agar gel or on a reaction strip containing urea, a buffer,and a pH-sensitive indicator.
- The urea is metabolized to ammonia and bicarbonate in the presence of H.pylori's urease leading to a pH increase in the microenvironment of the organism.
- A change in color of the pH sensitive indicator signifies the presence of the active infection.
- The sensitivity of the RUT decreases due to medications such as bismuth-containing compounds, antibiotics, or PPIs which reduce the density and/or urease activity of H.pylori.
- It is recommended that biopsies are taken from two sites due to patchy distribution of H.pylori infection after antibiotics use. The sites include:
- The body at the gastric angularis
- Greater curvature of the antrum
- PPIs are withheld for 1-2 wk before performance of RUT as they reduce the sensitivity of test.
Culture
- Culture is not as sensitive as RUT or histology.
- Highly specific method for identifying active H.pylori infection.
- It is also used to determine antibiotic sensitivities and resistance.
Polymerase Chain Reaction (PCR)
- PCR is highly specific and more sensitive than other biopsy-based diagnostic techniques.
- It is also used to identify mutations associated with antimicrobial resistance.
- This method is not standardized across laboratories
For more information on endoscopic diagnostic methods please click here