CRSP3: Difference between revisions

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{{Infobox_gene}}
{{PBB_Controls
'''Mediator of RNA polymerase II transcription subunit 23''' is an [[enzyme]] that in humans is encoded by the ''MED23'' [[gene]].<ref name="pmid9989412">{{cite journal | vauthors = Ryu S, Zhou S, Ladurner AG, Tjian R | title = The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1 | journal = Nature | volume = 397 | issue = 6718 | pages = 446–50 | date = Feb 1999 | pmid = 9989412 | pmc =  | doi = 10.1038/17141 }}</ref><ref name="entrez">{{cite web | title = Entrez Gene: CRSP3 cofactor required for Sp1 transcriptional activation, subunit 3, 130kDa| url = https://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9439| accessdate = }}</ref>
| update_page = yes
| require_manual_inspection = no
| update_protein_box = yes
| update_summary = no
| update_citations = yes
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<!-- The GNF_Protein_box is automatically maintained by Protein Box Bot.  See Template:PBB_Controls to Stop updates. -->
== Function ==
{{GNF_Protein_box
| image =
| image_source =
| PDB =
| Name = Cofactor required for Sp1 transcriptional activation, subunit 3, 130kDa
| HGNCid = 2372
| Symbol = CRSP3
| AltSymbols =; CRSP130; CRSP133; DKFZp434H0117; DRIP130; MED23; SUR2
| OMIM = 605042
| ECnumber = 
| Homologene = 3552
| MGIid = 1917458
| GeneAtlas_image1 = PBB_GE_CRSP3_218846_at_tn.png
| Function = {{GNF_GO|id=GO:0003713 |text = transcription coactivator activity}} {{GNF_GO|id=GO:0004872 |text = receptor activity}} {{GNF_GO|id=GO:0030528 |text = transcription regulator activity}}
| Component = {{GNF_GO|id=GO:0005667 |text = transcription factor complex}}
| Process = {{GNF_GO|id=GO:0006350 |text = transcription}} {{GNF_GO|id=GO:0006357 |text = regulation of transcription from RNA polymerase II promoter}} {{GNF_GO|id=GO:0006367 |text = transcription initiation from RNA polymerase II promoter}}
| Orthologs = {{GNF_Ortholog_box
    | Hs_EntrezGene = 9439
    | Hs_Ensembl = ENSG00000112282
    | Hs_RefseqProtein = NP_004821
    | Hs_RefseqmRNA = NM_004830
    | Hs_GenLoc_db = 
    | Hs_GenLoc_chr = 6
    | Hs_GenLoc_start = 131936801
    | Hs_GenLoc_end = 131991062
    | Hs_Uniprot = Q9ULK4
    | Mm_EntrezGene = 70208
    | Mm_Ensembl = ENSMUSG00000019984
    | Mm_RefseqmRNA = NM_027347
    | Mm_RefseqProtein = NP_081623
    | Mm_GenLoc_db = 
    | Mm_GenLoc_chr = 10
    | Mm_GenLoc_start = 24559463
    | Mm_GenLoc_end = 24602876
    | Mm_Uniprot = 
  }}
}}
'''Cofactor required for Sp1 transcriptional activation, subunit 3, 130kDa''', also known as '''CRSP3''', is a human [[gene]].<ref name="entrez">{{cite web | title = Entrez Gene: CRSP3 cofactor required for Sp1 transcriptional activation, subunit 3, 130kDa| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9439| accessdate = }}</ref>


<!-- The PBB_Summary template is automatically maintained by Protein Box Bot.  See Template:PBB_Controls to Stop updates. -->
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a [[metastasis suppressor]]. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.<ref name="entrez"/>
{{PBB_Summary
| section_title =
| summary_text = The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a [[metastasis suppressor]]. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.<ref name="entrez">{{cite web | title = Entrez Gene: CRSP3 cofactor required for Sp1 transcriptional activation, subunit 3, 130kDa| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9439| accessdate = }}</ref>
}}


==References==
== Interactions ==
{{reflist|2}}
 
==Further reading==
CRSP3 has been shown to [[Protein-protein interaction|interact]] with [[Estrogen receptor alpha]],<ref name=pmid11867769>{{cite journal | vauthors = Kang YK, Guermah M, Yuan CX, Roeder RG | title = The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 99 | issue = 5 | pages = 2642–7 | date = Mar 2002 | pmid = 11867769 | pmc = 122401 | doi = 10.1073/pnas.261715899 }}</ref> [[CEBPB]]<ref name=pmid14759369>{{cite journal | vauthors = Mo X, Kowenz-Leutz E, Xu H, Leutz A | title = Ras induces mediator complex exchange on C/EBP beta | journal = Molecular Cell | volume = 13 | issue = 2 | pages = 241–50 | date = Jan 2004 | pmid = 14759369 | doi = 10.1016/S1097-2765(03)00521-5 }}</ref> and [[Cyclin-dependent kinase 8]].<ref name=pmid11867769/><ref name=pmid11416138>{{cite journal | vauthors = Wang G, Cantin GT, Stevens JL, Berk AJ | title = Characterization of mediator complexes from HeLa cell nuclear extract | journal = Molecular and Cellular Biology | volume = 21 | issue = 14 | pages = 4604–13 | date = Jul 2001 | pmid = 11416138 | pmc = 87123 | doi = 10.1128/MCB.21.14.4604-4613.2001 }}</ref>
 
== References ==
{{reflist}}
 
==External links==
* {{UCSC gene info|MED23}}
 
== Further reading ==
{{refbegin | 2}}
{{refbegin | 2}}
{{PBB_Further_reading
* {{cite journal | vauthors = Rachez C, Lemon BD, Suldan Z, Bromleigh V, Gamble M, Näär AM, Erdjument-Bromage H, Tempst P, Freedman LP | title = Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex | journal = Nature | volume = 398 | issue = 6730 | pages = 824–8 | date = Apr 1999 | pmid = 10235266 | doi = 10.1038/19783 }}
| citations =
* {{cite journal | vauthors = Näär AM, Beaurang PA, Zhou S, Abraham S, Solomon W, Tjian R | title = Composite co-activator ARC mediates chromatin-directed transcriptional activation | journal = Nature | volume = 398 | issue = 6730 | pages = 828–32 | date = Apr 1999 | pmid = 10235267 | doi = 10.1038/19789 }}
*{{cite journal  | author=Ryu S, Zhou S, Ladurner AG, Tjian R |title=The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1. |journal=Nature |volume=397 |issue= 6718 |pages= 446-50 |year= 1999 |pmid= 9989412 |doi= 10.1038/17141 }}
* {{cite journal | vauthors = Boyer TG, Martin ME, Lees E, Ricciardi RP, Berk AJ | title = Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein | journal = Nature | volume = 399 | issue = 6733 | pages = 276–9 | date = May 1999 | pmid = 10353252 | doi = 10.1038/20466 }}
*{{cite journal | author=Rachez C, Lemon BD, Suldan Z, ''et al.'' |title=Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. |journal=Nature |volume=398 |issue= 6730 |pages= 824-8 |year= 1999 |pmid= 10235266 |doi= 10.1038/19783 }}
* {{cite journal | vauthors = Nagase T, Ishikawa K, Kikuno R, Hirosawa M, Nomura N, Ohara O | title = Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro | journal = DNA Research | volume = 6 | issue = 5 | pages = 337–45 | date = Oct 1999 | pmid = 10574462 | doi = 10.1093/dnares/6.5.337 }}
*{{cite journal | author=Näär AM, Beaurang PA, Zhou S, ''et al.'' |title=Composite co-activator ARC mediates chromatin-directed transcriptional activation. |journal=Nature |volume=398 |issue= 6730 |pages= 828-32 |year= 1999 |pmid= 10235267 |doi= 10.1038/19789 }}
* {{cite journal | vauthors = Ko L, Cardona GR, Chin WW | title = Thyroid hormone receptor-binding protein, an LXXLL motif-containing protein, functions as a general coactivator | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 97 | issue = 11 | pages = 6212–7 | date = May 2000 | pmid = 10823961 | pmc = 18584 | doi = 10.1073/pnas.97.11.6212 }}
*{{cite journal | author=Boyer TG, Martin ME, Lees E, ''et al.'' |title=Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein. |journal=Nature |volume=399 |issue= 6733 |pages= 276-9 |year= 1999 |pmid= 10353252 |doi= 10.1038/20466 }}
* {{cite journal | vauthors = Hartley JL, Temple GF, Brasch MA | title = DNA cloning using in vitro site-specific recombination | journal = Genome Research | volume = 10 | issue = 11 | pages = 1788–95 | date = Nov 2000 | pmid = 11076863 | pmc = 310948 | doi = 10.1101/gr.143000 }}
*{{cite journal | author=Nagase T, Ishikawa K, Kikuno R, ''et al.'' |title=Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. |journal=DNA Res. |volume=6 |issue= 5 |pages= 337-45 |year= 2000 |pmid= 10574462 |doi= }}
* {{cite journal | vauthors = Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A | title = Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs | journal = Genome Research | volume = 11 | issue = 3 | pages = 422–35 | date = Mar 2001 | pmid = 11230166 | pmc = 311072 | doi = 10.1101/gr.GR1547R }}
*{{cite journal | author=Ko L, Cardona GR, Chin WW |title=Thyroid hormone receptor-binding protein, an LXXLL motif-containing protein, functions as a general coactivator. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=97 |issue= 11 |pages= 6212-7 |year= 2000 |pmid= 10823961 |doi= }}
* {{cite journal | vauthors = Stevens JL, Cantin GT, Wang G, Shevchenko A, Shevchenko A, Berk AJ | title = Transcription control by E1A and MAP kinase pathway via Sur2 mediator subunit | journal = Science | volume = 296 | issue = 5568 | pages = 755–8 | date = Apr 2002 | pmid = 11934987 | doi = 10.1126/science.1068943 }}
*{{cite journal | author=Hartley JL, Temple GF, Brasch MA |title=DNA cloning using in vitro site-specific recombination. |journal=Genome Res. |volume=10 |issue= 11 |pages= 1788-95 |year= 2001 |pmid= 11076863 |doi= }}
* {{cite journal | vauthors = Asada S, Choi Y, Yamada M, Wang SC, Hung MC, Qin J, Uesugi M | title = External control of Her2 expression and cancer cell growth by targeting a Ras-linked coactivator | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 99 | issue = 20 | pages = 12747–52 | date = Oct 2002 | pmid = 12242338 | pmc = 130531 | doi = 10.1073/pnas.202162199 }}
*{{cite journal | author=Wiemann S, Weil B, Wellenreuther R, ''et al.'' |title=Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. |journal=Genome Res. |volume=11 |issue= 3 |pages= 422-35 |year= 2001 |pmid= 11230166 |doi= 10.1101/gr.154701 }}
* {{cite journal | vauthors = Nakayama M, Kikuno R, Ohara O | title = Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs | journal = Genome Research | volume = 12 | issue = 11 | pages = 1773–84 | date = Nov 2002 | pmid = 12421765 | pmc = 187542 | doi = 10.1101/gr.406902 }}
*{{cite journal | author=Stevens JL, Cantin GT, Wang G, ''et al.'' |title=Transcription control by E1A and MAP kinase pathway via Sur2 mediator subunit. |journal=Science |volume=296 |issue= 5568 |pages= 755-8 |year= 2002 |pmid= 11934987 |doi= 10.1126/science.1068943 }}
* {{cite journal | vauthors = Goldberg SF, Miele ME, Hatta N, Takata M, Paquette-Straub C, Freedman LP, Welch DR | title = Melanoma metastasis suppression by chromosome 6: evidence for a pathway regulated by CRSP3 and TXNIP | journal = Cancer Research | volume = 63 | issue = 2 | pages = 432–40 | date = Jan 2003 | pmid = 12543799 | doi =  }}
*{{cite journal | author=Asada S, Choi Y, Yamada M, ''et al.'' |title=External control of Her2 expression and cancer cell growth by targeting a Ras-linked coactivator. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 20 |pages= 12747-52 |year= 2002 |pmid= 12242338 |doi= 10.1073/pnas.202162199 }}
* {{cite journal | vauthors = Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower CS, Conaway RC, Conaway JW | title = A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex | journal = The Journal of Biological Chemistry | volume = 278 | issue = 50 | pages = 49671–4 | date = Dec 2003 | pmid = 14576168 | doi = 10.1074/jbc.C300444200 }}
*{{cite journal | author=Nakayama M, Kikuno R, Ohara O |title=Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs. |journal=Genome Res. |volume=12 |issue= 11 |pages= 1773-84 |year= 2003 |pmid= 12421765 |doi= 10.1101/gr.406902 }}
* {{cite journal | vauthors = Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW | title = A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2 | journal = The Journal of Biological Chemistry | volume = 279 | issue = 7 | pages = 5846–51 | date = Feb 2004 | pmid = 14638676 | doi = 10.1074/jbc.M312523200 }}
*{{cite journal | author=Strausberg RL, Feingold EA, Grouse LH, ''et al.'' |title=Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. |journal=Proc. Natl. Acad. Sci. U.S.A. |volume=99 |issue= 26 |pages= 16899-903 |year= 2003 |pmid= 12477932 |doi= 10.1073/pnas.242603899 }}
* {{cite journal | vauthors = Mo X, Kowenz-Leutz E, Xu H, Leutz A | title = Ras induces mediator complex exchange on C/EBP beta | journal = Molecular Cell | volume = 13 | issue = 2 | pages = 241–50 | date = Jan 2004 | pmid = 14759369 | doi = 10.1016/S1097-2765(03)00521-5 }}
*{{cite journal  | author=Goldberg SF, Miele ME, Hatta N, ''et al.'' |title=Melanoma metastasis suppression by chromosome 6: evidence for a pathway regulated by CRSP3 and TXNIP. |journal=Cancer Res. |volume=63 |issue= 2 |pages= 432-40 |year= 2003 |pmid= 12543799 |doi=  }}
* {{cite journal | vauthors = Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC | title = A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology | journal = Molecular Cell | volume = 14 | issue = 5 | pages = 685–91 | date = Jun 2004 | pmid = 15175163 | doi = 10.1016/j.molcel.2004.05.006 }}
*{{cite journal | author=Mungall AJ, Palmer SA, Sims SK, ''et al.'' |title=The DNA sequence and analysis of human chromosome 6. |journal=Nature |volume=425 |issue= 6960 |pages= 805-11 |year= 2003 |pmid= 14574404 |doi= 10.1038/nature02055 }}
* {{cite journal | vauthors = Wada O, Oishi H, Takada I, Yanagisawa J, Yano T, Kato S | title = BRCA1 function mediates a TRAP/DRIP complex through direct interaction with TRAP220 | journal = Oncogene | volume = 23 | issue = 35 | pages = 6000–5 | date = Aug 2004 | pmid = 15208681 | doi = 10.1038/sj.onc.1207786 }}
*{{cite journal  | author=Sato S, Tomomori-Sato C, Banks CA, ''et al.'' |title=A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex. |journal=J. Biol. Chem. |volume=278 |issue= 50 |pages= 49671-4 |year= 2004 |pmid= 14576168 |doi= 10.1074/jbc.C300444200 }}
* {{cite journal | vauthors = Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A | title = From ORFeome to biology: a functional genomics pipeline | journal = Genome Research | volume = 14 | issue = 10B | pages = 2136–44 | date = Oct 2004 | pmid = 15489336 | pmc = 528930 | doi = 10.1101/gr.2576704 }}
*{{cite journal | author=Tomomori-Sato C, Sato S, Parmely TJ, ''et al.'' |title=A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. |journal=J. Biol. Chem. |volume=279 |issue= 7 |pages= 5846-51 |year= 2004 |pmid= 14638676 |doi= 10.1074/jbc.M312523200 }}
*{{cite journal | author=Mo X, Kowenz-Leutz E, Xu H, Leutz A |title=Ras induces mediator complex exchange on C/EBP beta. |journal=Mol. Cell |volume=13 |issue= 2 |pages= 241-50 |year= 2004 |pmid= 14759369 |doi= }}
*{{cite journal | author=Sato S, Tomomori-Sato C, Parmely TJ, ''et al.'' |title=A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. |journal=Mol. Cell |volume=14 |issue= 5 |pages= 685-91 |year= 2004 |pmid= 15175163 |doi= 10.1016/j.molcel.2004.05.006 }}
*{{cite journal | author=Wada O, Oishi H, Takada I, ''et al.'' |title=BRCA1 function mediates a TRAP/DRIP complex through direct interaction with TRAP220. |journal=Oncogene |volume=23 |issue= 35 |pages= 6000-5 |year= 2004 |pmid= 15208681 |doi= 10.1038/sj.onc.1207786 }}
*{{cite journal | author=Wiemann S, Arlt D, Huber W, ''et al.'' |title=From ORFeome to biology: a functional genomics pipeline. |journal=Genome Res. |volume=14 |issue= 10B |pages= 2136-44 |year= 2004 |pmid= 15489336 |doi= 10.1101/gr.2576704 }}
}}
{{refend}}
{{refend}}


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Latest revision as of 10:03, 30 August 2017

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Identifiers
Aliases
External IDsGeneCards: [1]
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

n/a

n/a

RefSeq (protein)

n/a

n/a

Location (UCSC)n/an/a
PubMed searchn/an/a
Wikidata
View/Edit Human

Mediator of RNA polymerase II transcription subunit 23 is an enzyme that in humans is encoded by the MED23 gene.[1][2]

Function

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.[2]

Interactions

CRSP3 has been shown to interact with Estrogen receptor alpha,[3] CEBPB[4] and Cyclin-dependent kinase 8.[3][5]

References

  1. Ryu S, Zhou S, Ladurner AG, Tjian R (Feb 1999). "The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1". Nature. 397 (6718): 446–50. doi:10.1038/17141. PMID 9989412.
  2. 2.0 2.1 "Entrez Gene: CRSP3 cofactor required for Sp1 transcriptional activation, subunit 3, 130kDa".
  3. 3.0 3.1 Kang YK, Guermah M, Yuan CX, Roeder RG (Mar 2002). "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro". Proceedings of the National Academy of Sciences of the United States of America. 99 (5): 2642–7. doi:10.1073/pnas.261715899. PMC 122401. PMID 11867769.
  4. Mo X, Kowenz-Leutz E, Xu H, Leutz A (Jan 2004). "Ras induces mediator complex exchange on C/EBP beta". Molecular Cell. 13 (2): 241–50. doi:10.1016/S1097-2765(03)00521-5. PMID 14759369.
  5. Wang G, Cantin GT, Stevens JL, Berk AJ (Jul 2001). "Characterization of mediator complexes from HeLa cell nuclear extract". Molecular and Cellular Biology. 21 (14): 4604–13. doi:10.1128/MCB.21.14.4604-4613.2001. PMC 87123. PMID 11416138.

External links

Further reading