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[[Image:Cresol red.png|right|150px|thumb|chemical structure of cresol red]]
{{Chembox new
|  Name = Cresol Red
|  ImageFile = Cresol Red.svg
<!-- | ImageSize = 200px  -->
|  IUPACName =
| Section1 = {{Chembox Identifiers
|   CASNo = 1733-12-6
|   SMILES = Cc1cc(ccc1O)/C(=C/2C=CC(=O)C(=C2)C)c3ccccc3OS(O)=O
  }}
| Section2 = {{Chembox Properties
|  Formula = C<sub>21</sub>H<sub>17</sub>NaO<sub>5</sub>S
|  MolarMass = 404.41 g/mol
|  Density =
|  MeltingPt =
|  BoilingPt =
  }}
}}
{{pH_indicator_template|indicator_name=Cresol  Red|low_pH=7.2|high_pH=8.8|low_pH_color=yellow|high_pH_color=#800080}}
{{SI}}


[[Image:Agarose gel dyes.jpg|right|150px]]
{{EH}}


'''Cresol red''' is a pH indicator and [[Agarose_gel_loading_dye#Colour|molecular weight marker for agarose gels]]. Unlike [[bromophenol blue]] and [[xylene cyanol]], cresol red does not inhibit Taq polymerase in PCRs.
'''Cresol Red''' (full name: o-Cresolsulfonephthalein) is a triarylmethane dye frequently used for monitoring the pH in aquaria.


== Agarose molecular weight marker ==
Cresol Red can be used in many molecular reactions instead of water. For example restriction digestion or PCR.
advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue


* molecular weight 404 g/mol
Cresol Red can also be used as a color marker to monitor the process of [[agarose gel electrophoresis]] and [[polyacrylamide gel electrophoresis]]. In a 1% agarose gel it runs approximately at the size of a 125 base pair (bp) [[DNA]] molecule (it depends on the concentration of buffer and other component). [[Bromophenol blue]] and [[xylene cyanol]] can also be used for this purpose.
* in 1% agarose at ~1000 bp (between xylene cyanol 5000bp and bromophenol blue 500bp)
* in 3% agarose at >100bp (xylene cyanol 300bp)


== pH indicator ==
==References==
* amber - acidic pH
<references/>
* yellow - neutral
* red - alkaline


== External links ==
* [[Image:3stars.png]] [http://www.wendychao.com/science/protocols/loading_buffer/ Wendy Chao's loading dye recipe and discussion]
* [http://molbiol.ru/eng/protocol/01_03.html#a5 Cresol Red solution calculator]
* [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=73160&loc=ec_rcs Cresol red at Pubchem]
* [http://www.sigmaaldrich.com/catalog/ProductDetail.do?N4=114480|Aldrich&N5=Product%20No.|BRAND_KEY&F=SPEC Cresol Red sodium salt - Sigma catalogue]
* [http://www.sigmaaldrich.com/life-science/cell-biology/hematology-and-histology/hematology-and-histology-products.html?TablePage=17895485 Stains and Dyes Letter C of the Sigma Aldrich online catalogue showing different Cresol varieties]
* [http://en.wikipedia.org/wiki/Cresol_Red Cresol Red at Wikipedia]


[[Category:Material]] [[Category:Chemical]] [[Category:agarose gel electrophoresis]]
{{SIB}}
 
[[Category:Triarylmethane dyes]]
 
[[de:Kresolrot]]
[[fr:Rouge de crésol]]
[[it:Rosso cresolo]]
[[ja:クレゾールレッド]]
[[pl:Czerwień krezolowa]]
[[zh:甲酚紅]]
 
 
{{WH}}
{{WS}}

Revision as of 21:00, 9 July 2009

Template:Chembox new Template:PH indicator template

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Cresol Red (full name: o-Cresolsulfonephthalein) is a triarylmethane dye frequently used for monitoring the pH in aquaria.

Cresol Red can be used in many molecular reactions instead of water. For example restriction digestion or PCR.

Cresol Red can also be used as a color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. In a 1% agarose gel it runs approximately at the size of a 125 base pair (bp) DNA molecule (it depends on the concentration of buffer and other component). Bromophenol blue and xylene cyanol can also be used for this purpose.

References


Template:SIB

de:Kresolrot it:Rosso cresolo


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