Cresol Red: Difference between revisions

Revision as of 20:47, 9 July 2009

chemical structure of cresol red

Cresol red is a pH indicator and molecular weight marker for agarose gels. Unlike bromophenol blue and xylene cyanol, cresol red does not inhibit Taq polymerase in PCRs.

advantage: much less shadow on EtBr pictures unlike xylene cyanol and bromophenol blue

molecular weight 404 g/mol
in 1% agarose at ~1000 bp (between xylene cyanol 5000bp and bromophenol blue 500bp)
in 3% agarose at >100bp (xylene cyanol 300bp)

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(No difference)