SARAF (TMEM66) is a negative regulator of the [[ICRAC|store-operated calcium channel]] (SOCE) into cells. SARAF is an [[endoplasmic reticulum]] (ER) membrane resident protein that associates with [[STIM1]], to facilitate the inactivation of SOCE. SARAF plays a key role in shaping [[cytoplasmic]] [[calcium in biology|calcium]] signals and determining the content of the major intracellular Ca<sup>2+</sup> stores in the cell. By doing so it is likely to be important in protecting cells from calcium overfilling.<ref name=Palty_2012>{{cite journal | vauthors = Palty R, Raveh A, Kaminsky I, Meller R, Reuveny E | title = SARAF Inactivates the Store Operated Calcium Entry Machinery to Prevent Excess Calcium Refilling | journal = Cell | year = 2012 | volume = 149 | doi = 10.1016/j.cell.2012.01.055 | pmid=22464749 | pages=425–38}}</ref>
SARAF (TMEM66) is a negative regulator of the [[Calcium release activated channel|store-operated calcium channel]] (SOCE) into cells. SARAF is an [[endoplasmic reticulum]] (ER) membrane resident protein that associates with [[STIM1]], to facilitate the inactivation of SOCE. SARAF plays a key role in shaping [[cytoplasmic]] [[calcium in biology|calcium]] signals and determining the content of the major intracellular Ca<sup>2+</sup> stores in the cell. By doing so it is likely to be important in protecting cells from calcium overfilling.<ref name=Palty_2012>{{cite journal | vauthors = Palty R, Raveh A, Kaminsky I, Meller R, Reuveny E | title = SARAF Inactivates the Store Operated Calcium Entry Machinery to Prevent Excess Calcium Refilling | journal = Cell | year = 2012 | volume = 149 | doi = 10.1016/j.cell.2012.01.055 | pmid=22464749 | pages=425–38}}</ref>
SARAF is a protein that in humans is encoded by the SARAFgene, formerly known as TMEM66 (transmembrane protein 66).[1][2]
Function
SARAF (TMEM66) is a negative regulator of the store-operated calcium channel (SOCE) into cells. SARAF is an endoplasmic reticulum (ER) membrane resident protein that associates with STIM1, to facilitate the inactivation of SOCE. SARAF plays a key role in shaping cytoplasmiccalcium signals and determining the content of the major intracellular Ca2+ stores in the cell. By doing so it is likely to be important in protecting cells from calcium overfilling.[3]
↑Palty R, Raveh A, Kaminsky I, Meller R, Reuveny E (2012). "SARAF Inactivates the Store Operated Calcium Entry Machinery to Prevent Excess Calcium Refilling". Cell. 149: 425–38. doi:10.1016/j.cell.2012.01.055. PMID22464749.
Further reading
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID9373149.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID14702039.
Otsuki T, Ota T, Nishikawa T, et al. (2007). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries". DNA Res. 12 (2): 117–26. doi:10.1093/dnares/12.2.117. PMID16303743.
