Nitrate reductase test: Difference between revisions
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Latest revision as of 14:19, 20 August 2012
The nitrate reductase test is a test to differentiate between bacteria based on their ability or inability to reduce nitrate (NO3) to nitrite (NO2) using anaerobic respiration.
Procedure:
- Inoculate nitrate broth with an isolate and incubate for 48 hours.
- Add 10-15 drops each of sulfanilic acid and N,N-dimethyl-1-naphthylamine. If the bacterium produces nitrate reductase, the broth will turn a deep red within 5 minutes at this step.
- If no color change is observed, then the result is inconclusive. Add a small amount of zinc to the broth. If the solution remains colorless, then both nitrate and nitrite reductase are present. If the solution turns red, nitrate reductase is not present.