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{{Infobox_gene}}
{{Infobox_gene}}
'''Pyruvate kinase isozymes M1/M2''' (PKM1/M2), also known as '''pyruvate kinase muscle isozyme''' (PKM), '''pyruvate kinase type K''', '''cytosolic thyroid hormone-binding protein''' (CTHBP),  '''thyroid hormone-binding protein 1''' (THBP1), or '''opa-interacting protein 3''' (OIP3), is an [[enzyme]] that in humans is encoded by the ''PKM2'' [[gene]].<ref name="pmid3818670">{{cite journal | vauthors = Kitagawa S, Obata T, Hasumura S, Pastan I, Cheng SY | title = A cellular 3,3',5-triiodo-L-thyronine binding protein from a human carcinoma cell line. Purification and characterization | journal = The Journal of Biological Chemistry | volume = 262 | issue = 8 | pages = 3903–8 | date = Mar 1987 | pmid = 3818670 | doi =  }}</ref><ref name="pmid2838416">{{cite journal | vauthors = Tsutsumi H, Tani K, Fujii H, Miwa S | title = Expression of L- and M-type pyruvate kinase in human tissues | journal = Genomics | volume = 2 | issue = 1 | pages = 86–9 | date = Jan 1988 | pmid = 2838416 | doi = 10.1016/0888-7543(88)90112-7 }}</ref><ref name="pmid2854097">{{cite journal | vauthors = Tani K, Yoshida MC, Satoh H, Mitamura K, Noguchi T, Tanaka T, Fujii H, Miwa S | title = Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma | journal = Gene | volume = 73 | issue = 2 | pages = 509–16 | date = Dec 1988 | pmid = 2854097 | doi = 10.1016/0378-1119(88)90515-X }}</ref><ref name="pmid2267632">{{cite journal | vauthors = Popescu NC, Cheng SY | title = Chromosomal localization of the gene for a human cytosolic thyroid hormone binding protein homologous to the subunit of pyruvate kinase, subtype M2 | journal = Somatic Cell and Molecular Genetics | volume = 16 | issue = 6 | pages = 593–8 | date = Nov 1990 | pmid = 2267632 | doi = 10.1007/BF01233100 }}</ref>
'''Pyruvate kinase isozymes M1/M2''' (PKM1/M2), also known as '''pyruvate kinase muscle isozyme''' (PKM), '''pyruvate kinase type K''', '''cytosolic thyroid hormone-binding protein''' (CTHBP),  '''thyroid hormone-binding protein 1''' (THBP1), or '''opa-interacting protein 3''' (OIP3), is an [[enzyme]] that in humans is encoded by the ''PKM2'' [[gene]].<ref name="pmid3818670">{{cite journal | vauthors = Kitagawa S, Obata T, Hasumura S, Pastan I, Cheng SY | title = A cellular 3,3',5-triiodo-L-thyronine binding protein from a human carcinoma cell line. Purification and characterization | journal = The Journal of Biological Chemistry | volume = 262 | issue = 8 | pages = 3903–8 | date = March 1987 | pmid = 3818670 | doi =  }}</ref><ref name="pmid2838416">{{cite journal | vauthors = Tsutsumi H, Tani K, Fujii H, Miwa S | title = Expression of L- and M-type pyruvate kinase in human tissues | journal = Genomics | volume = 2 | issue = 1 | pages = 86–9 | date = January 1988 | pmid = 2838416 | doi = 10.1016/0888-7543(88)90112-7 }}</ref><ref name="pmid2854097">{{cite journal | vauthors = Tani K, Yoshida MC, Satoh H, Mitamura K, Noguchi T, Tanaka T, Fujii H, Miwa S | title = Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma | journal = Gene | volume = 73 | issue = 2 | pages = 509–16 | date = December 1988 | pmid = 2854097 | doi = 10.1016/0378-1119(88)90515-X }}</ref><ref name="pmid2267632">{{cite journal | vauthors = Popescu NC, Cheng SY | title = Chromosomal localization of the gene for a human cytosolic thyroid hormone binding protein homologous to the subunit of pyruvate kinase, subtype M2 | journal = Somatic Cell and Molecular Genetics | volume = 16 | issue = 6 | pages = 593–8 | date = November 1990 | pmid = 2267632 | doi = 10.1007/BF01233100 }}</ref>


PKM2 is an [[isoenzyme]] of the [[glycolysis|glycolytic]] [[enzyme]] [[pyruvate kinase]]. Depending upon the different metabolic functions of the tissues, different isoenzymes of pyruvate kinase are expressed. PKM2 is expressed in some differentiated tissues, such as [[lung]], [[fat]] tissue, [[retina]], and [[pancreatic islet]]s, as well as in all cells with a high rate of [[DNA synthesis|nucleic acid synthesis]], such as normal proliferating cells, [[embryonic cell]]s, and especially [[tumor]] cells.<ref name="Corcoran">{{cite journal | vauthors = Corcoran E, Phelan JJ, Fottrell PF | title = Purification and properties of pyruvate kinase from human lung | journal = Biochimica et Biophysica Acta | volume = 446 | issue = 1 | pages = 96–104 | date = Sep 1976 | pmid = 974119 | doi = 10.1016/0005-2795(76)90101-x }}</ref><ref name="Tolle">{{cite journal | vauthors = Tolle SW, Dyson RD, Newburgh RW, Cardenas JM | title = Pyruvate kinase isozymes in neurons, glia, neuroblastoma, and glioblastoma | journal = Journal of Neurochemistry | volume = 27 | issue = 6 | pages = 1355–1360 | date = Dec 1976 | pmid = 1003209 | doi = 10.1111/j.1471-4159.1976.tb02615.x }}</ref><ref name="Reinacher">{{cite journal | vauthors = Reinacher M, Eigenbrodt E | title = Immunohistological demonstration of the same type of pyruvate kinase isoenzyme (M2-Pk) in tumors of chicken and rat | journal = Virchows Archiv B | volume = 37 | issue = 1 | pages = 79–88 | year = 1981 | pmid = 6116351 | doi = 10.1007/BF02892557 }}</ref><ref name="Schering">{{cite journal | vauthors = Schering B, Eigenbrodt E, Linder D, Schoner W | title = Purification and properties of pyruvate kinase type M2 from rat lung | journal = Biochimica et Biophysica Acta | volume = 717 | issue = 2 | pages = 337–347 | date = Aug 1982 | pmid = 7115773 | doi = 10.1016/0304-4165(82)90188-X }}</ref><ref name="[[Mac Donald]]">{{cite journal | vauthors = MacDonald MJ, Chang CM | title = Pancreatic islets contain the M2 isoenzyme of pyruvate kinase. Its phosphorylation has no effect on enzyme activity | journal = Molecular and Cellular Biochemistry | volume = 68 | issue = 2 | pages = 115–120 | date = Oct 1985 | pmid = 3908905 | doi = 10.1007/bf00219375 }}</ref><ref name="Brinck">{{cite journal | vauthors = Brinck U, Eigenbrodt E, Oehmke M, Mazurek S, Fischer G | title = L- and M2-pyruvate kinase expression in renal cell carcinomas and their metastases | journal = Virchows Archiv | volume = 424 | issue = 2 | pages = 177–185 | year = 1994 | pmid = 8180780 | doi = 10.1007/BF00193498 }}</ref><ref name="Steinberg">{{cite journal | vauthors = Steinberg P, Klingelhöffer A, Schäfer A, Wüst G, Weisse G, Oesch F, Eigenbrodt E | title = Expression of pyruvate kinase M2 in preneoplastic hepatic foci of N-nitrosomorpholine-treated rats | journal = Virchows Archiv | volume = 434 | issue = 3 | pages = 213–220 | date = Mar 1999 | pmid = 10190300 | doi = 10.1007/s004280050330 }}</ref>
PKM2 is an [[isoenzyme]] of the [[glycolysis|glycolytic]] [[enzyme]] [[pyruvate kinase]]. Depending upon the different metabolic functions of the tissues, different isoenzymes of pyruvate kinase are expressed. PKM2 is expressed in some differentiated tissues, such as [[lung]], [[fat]] tissue, [[retina]], and [[pancreatic islet]]s, as well as in all cells with a high rate of [[DNA synthesis|nucleic acid synthesis]], such as normal proliferating cells, [[embryonic cell]]s, and especially [[tumor]] cells.<ref name="Corcoran">{{cite journal | vauthors = Corcoran E, Phelan JJ, Fottrell PF | title = Purification and properties of pyruvate kinase from human lung | journal = Biochimica et Biophysica Acta | volume = 446 | issue = 1 | pages = 96–104 | date = September 1976 | pmid = 974119 | doi = 10.1016/0005-2795(76)90101-x }}</ref><ref name="Tolle">{{cite journal | vauthors = Tolle SW, Dyson RD, Newburgh RW, Cardenas JM | title = Pyruvate kinase isozymes in neurons, glia, neuroblastoma, and glioblastoma | journal = Journal of Neurochemistry | volume = 27 | issue = 6 | pages = 1355–60 | date = December 1976 | pmid = 1003209 | doi = 10.1111/j.1471-4159.1976.tb02615.x }}</ref><ref name="Reinacher">{{cite journal | vauthors = Reinacher M, Eigenbrodt E | title = Immunohistological demonstration of the same type of pyruvate kinase isoenzyme (M2-Pk) in tumors of chicken and rat | journal = Virchows Archiv. B, Cell Pathology Including Molecular Pathology | volume = 37 | issue = 1 | pages = 79–88 | year = 1981 | pmid = 6116351 | doi = 10.1007/BF02892557 }}</ref><ref name="Schering">{{cite journal | vauthors = Schering B, Eigenbrodt E, Linder D, Schoner W | title = Purification and properties of pyruvate kinase type M2 from rat lung | journal = Biochimica et Biophysica Acta | volume = 717 | issue = 2 | pages = 337–47 | date = August 1982 | pmid = 7115773 | doi = 10.1016/0304-4165(82)90188-X }}</ref><ref name="[[Mac Donald]]">{{cite journal | vauthors = MacDonald MJ, Chang CM | title = Pancreatic islets contain the M2 isoenzyme of pyruvate kinase. Its phosphorylation has no effect on enzyme activity | journal = Molecular and Cellular Biochemistry | volume = 68 | issue = 2 | pages = 115–20 | date = October 1985 | pmid = 3908905 | doi = 10.1007/bf00219375 }}</ref><ref name="Brinck">{{cite journal | vauthors = Brinck U, Eigenbrodt E, Oehmke M, Mazurek S, Fischer G | title = L- and M2-pyruvate kinase expression in renal cell carcinomas and their metastases | journal = Virchows Archiv | volume = 424 | issue = 2 | pages = 177–85 | year = 1994 | pmid = 8180780 | doi = 10.1007/BF00193498 }}</ref><ref name="Steinberg">{{cite journal | vauthors = Steinberg P, Klingelhöffer A, Schäfer A, Wüst G, Weisse G, Oesch F, Eigenbrodt E | title = Expression of pyruvate kinase M2 in preneoplastic hepatic foci of N-nitrosomorpholine-treated rats | journal = Virchows Archiv | volume = 434 | issue = 3 | pages = 213–20 | date = March 1999 | pmid = 10190300 | doi = 10.1007/s004280050330 }}</ref>


== Structure ==
== Structure ==


Two isozymes are encoded by the ''PKM'' gene: PKM1 and PKM2. The M-gene consists of 12 [[exon]]s and 11 [[intron]]s. PKM1 and PKM2 are different [[alternative splicing|splicing]] products of the M-gene (exon 9 for PKM1 and exon 10 for PKM2) and solely differ in 23 amino acids within a 56-amino acid stretch (aa 378-434) at their [[C-terminal|carboxy terminus]].<ref name="Noguchi">{{cite journal | vauthors = Noguchi T, Inoue H, Tanaka T | title = The M1- and M2-type isozymes of rat pyruvate kinase are produced from the same gene by alternative RNA splicing | journal = The Journal of Biological Chemistry | volume = 261 | issue = 29 | pages = 13807–13812 | date = Oct 1986 | pmid = 3020052 }}</ref><ref name="pmid15996096">{{cite journal | vauthors = Dombrauckas JD, Santarsiero BD, Mesecar AD | title = Structural basis for tumor pyruvate kinase M2 allosteric regulation and catalysis | journal = Biochemistry | volume = 44 | issue = 27 | pages = 9417–29 | date = Jul 2005 | pmid = 15996096 | doi = 10.1021/bi0474923 }}</ref>
Two isozymes are encoded by the ''PKM'' gene: PKM1 and PKM2. The M-gene consists of 12 [[exon]]s and 11 [[intron]]s. PKM1 and PKM2 are different [[alternative splicing|splicing]] products of the M-gene (exon 9 for PKM1 and exon 10 for PKM2) and solely differ in 23 amino acids within a 56-amino acid stretch (aa 378-434) at their [[C-terminal|carboxy terminus]].<ref name="Noguchi">{{cite journal | vauthors = Noguchi T, Inoue H, Tanaka T | title = The M1- and M2-type isozymes of rat pyruvate kinase are produced from the same gene by alternative RNA splicing | journal = The Journal of Biological Chemistry | volume = 261 | issue = 29 | pages = 13807–12 | date = October 1986 | pmid = 3020052 }}</ref><ref name="pmid15996096">{{cite journal | vauthors = Dombrauckas JD, Santarsiero BD, Mesecar AD | title = Structural basis for tumor pyruvate kinase M2 allosteric regulation and catalysis | journal = Biochemistry | volume = 44 | issue = 27 | pages = 9417–29 | date = July 2005 | pmid = 15996096 | doi = 10.1021/bi0474923 }}</ref>


== Function ==
== Function ==
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[[Pyruvate kinase]] [[catalyze]]s the last step within [[glycolysis]], the de[[phosphorylation]] of [[phosphoenolpyruvate]] to [[pyruvate]], and is responsible for net [[Adenosine triphosphate|ATP]] production within the glycolytic sequence. In contrast to [[mitochondria]]l [[Cellular respiration|respiration]], energy regeneration by pyruvate kinase is independent from oxygen supply and allows survival of the organs under [[Tumor hypoxia|hypoxic]] conditions often found in solid tumors.<ref name="pmid15591417">{{cite journal | vauthors = Vaupel P, Harrison L | title = Tumor hypoxia: causative factors, compensatory mechanisms, and cellular response | journal = The Oncologist | volume = 9 Suppl 5 | issue =  | pages = 4–9 | year = 2004 | pmid = 15591417 | doi = 10.1634/theoncologist.9-90005-4 }}</ref>
[[Pyruvate kinase]] [[catalyze]]s the last step within [[glycolysis]], the de[[phosphorylation]] of [[phosphoenolpyruvate]] to [[pyruvate]], and is responsible for net [[Adenosine triphosphate|ATP]] production within the glycolytic sequence. In contrast to [[mitochondria]]l [[Cellular respiration|respiration]], energy regeneration by pyruvate kinase is independent from oxygen supply and allows survival of the organs under [[Tumor hypoxia|hypoxic]] conditions often found in solid tumors.<ref name="pmid15591417">{{cite journal | vauthors = Vaupel P, Harrison L | title = Tumor hypoxia: causative factors, compensatory mechanisms, and cellular response | journal = The Oncologist | volume = 9 Suppl 5 | issue =  | pages = 4–9 | year = 2004 | pmid = 15591417 | doi = 10.1634/theoncologist.9-90005-4 }}</ref>


The involvement of this enzyme in a variety of [[signal transduction|pathways]], [[protein–protein interaction]]s, and nuclear transport suggests its potential to perform multiple nonglycolytic functions with diverse implications, although multidimensional role of this protein is as yet not fully explored. However, a functional role in [[angiogenesis]] the so-called process of blood vessel formation by interaction and regulation of [[Jmjd8]] has been shown.<ref name="pmid20857498">{{cite journal | vauthors = Gupta V, Bamezai RN | title = Human pyruvate kinase M2: a multifunctional protein | journal = Protein Science | volume = 19 | issue = 11 | pages = 2031–44 | date = Nov 2010 | pmid = 20857498 | pmc = 3005776 | doi = 10.1002/pro.505 }}</ref><ref name="Boeckel2016">{{cite journal | vauthors = Boeckel JN, Dimmeler S | title = JMJD8 Regulates Angiogenic Sprouting and Cellular Metabolism by Interacting With Pyruvate Kinase M2 in Endothelial Cells|journal =  Arteriosclerosis Thrombosis and Vascular Biology | year = 2016 | volume = Epub ahead of print| doi = 10.1161/ATVBAHA.116.307695 | pages=1425–1433}}</ref>
The involvement of this enzyme in a variety of [[signal transduction|pathways]], [[protein–protein interaction]]s, and nuclear transport suggests its potential to perform multiple nonglycolytic functions with diverse implications, although multidimensional role of this protein is as yet not fully explored. However, a functional role in [[angiogenesis]] the so-called process of blood vessel formation by interaction and regulation of [[Jmjd8]] has been shown.<ref name="pmid20857498">{{cite journal | vauthors = Gupta V, Bamezai RN | title = Human pyruvate kinase M2: a multifunctional protein | journal = Protein Science | volume = 19 | issue = 11 | pages = 2031–44 | date = November 2010 | pmid = 20857498 | pmc = 3005776 | doi = 10.1002/pro.505 }}</ref><ref name="Boeckel2016">{{cite journal | vauthors = Boeckel JN, Derlet A, Glaser SF, Luczak A, Lucas T, Heumüller AW, Krüger M, Zehendner CM, Kaluza D, Doddaballapur A, Ohtani K, Treguer K, Dimmeler S | title = JMJD8 Regulates Angiogenic Sprouting and Cellular Metabolism by Interacting With Pyruvate Kinase M2 in Endothelial Cells | journal = Arteriosclerosis, Thrombosis, and Vascular Biology | volume = 36 | issue = 7 | pages = 1425–33 | date = July 2016 | pmid = 27199445 | doi = 10.1161/ATVBAHA.116.307695 }}</ref>


== Localization ==
== Localization ==
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=== Tissue ===
=== Tissue ===


The PKM1 isozyme is expressed in organs that are strongly dependent upon a high rate of energy regeneration, such as [[muscle]] and [[brain]].<ref name="Eigenbrodt1980">{{cite journal | vauthors = Eigenbrodt E, Glossmann H | title = Glycolysis – one of the keys to cancer|journal = Trends Pharmacol. Sci. | year = 1980 | volume = 1 | issue = 2 | pages = 240–245| doi = 10.1016/0165-6147(80)90009-7 }}</ref><ref name="Eigenbrodt1992">{{cite journal | vauthors = Eigenbrodt E, Reinacher M, Scheefers-Borchel U, Scheefers H, Friis R | title = Double role for pyruvate kinase type M2 in the expansion of phosphometabolite pools found in tumor cells | journal = Critical Reviews in Oncogenesis | volume = 3 | issue = 1–2 | pages = 91–115 | year = 1992 | pmid = 1532331 }}</ref><ref name="Mazurek2005">{{cite journal | vauthors = Mazurek S, Boschek CB, Hugo F, Eigenbrodt E | title = Pyruvate kinase type M2 and its role in tumor growth and spreading | journal = Seminars in Cancer Biology | volume = 15 | issue = 4 | pages = 300–8 | date = Aug 2005 | pmid = 15908230 | doi = 10.1016/j.semcancer.2005.04.009 }}</ref>
The PKM1 isozyme is expressed in organs that are strongly dependent upon a high rate of energy regeneration, such as [[muscle]] and [[brain]].<ref name="Eigenbrodt1980">{{cite journal | vauthors = Eigenbrodt E, Glossmann H | title = Glycolysis – one of the keys to cancer|journal = Trends Pharmacol. Sci. | year = 1980 | volume = 1 | issue = 2 | pages = 240–245| doi = 10.1016/0165-6147(80)90009-7 }}</ref><ref name="Eigenbrodt1992">{{cite journal | vauthors = Eigenbrodt E, Reinacher M, Scheefers-Borchel U, Scheefers H, Friis R | title = Double role for pyruvate kinase type M2 in the expansion of phosphometabolite pools found in tumor cells | journal = Critical Reviews in Oncogenesis | volume = 3 | issue = 1-2 | pages = 91–115 | year = 1992 | pmid = 1532331 }}</ref><ref name="Mazurek2005">{{cite journal | vauthors = Mazurek S, Boschek CB, Hugo F, Eigenbrodt E | title = Pyruvate kinase type M2 and its role in tumor growth and spreading | journal = Seminars in Cancer Biology | volume = 15 | issue = 4 | pages = 300–8 | date = August 2005 | pmid = 15908230 | doi = 10.1016/j.semcancer.2005.04.009 }}</ref>


=== Subcellular ===
=== Subcellular ===


PKM2 is a [[cytosol]]ic enzyme that is associated with other glycolytic enzymes, i.e., [[hexokinase]], [[glyceraldehyde 3-phosphate dehydrogenase|glyceraldehyde 3-P dehydrogenase]], [[phosphoglycerate kinase]], [[phosphoglyceromutase]], [[enolase]], and [[lactate dehydrogenase]] within a so-called glycolytic enzyme complex.<ref name="Mazurek2005"/><ref name="Zwerschke">{{cite journal | vauthors = Zwerschke W, Mazurek S, Massimi P, Banks L, Eigenbrodt E, Jansen-Dürr P | title = Modulation of type M2 pyruvate kinase activity by the human papillomavirus type 16 E7 oncoprotein | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 96 | issue = 4 | pages = 1291–6 | date = Feb 1999 | pmid = 9990017 | pmc = 15456 | doi = 10.1073/pnas.96.4.1291 }}</ref><ref name="Mazurek2001">{{cite journal | vauthors = Mazurek S, Zwerschke W, Jansen-Dürr P, Eigenbrodt E | title = Metabolic cooperation between different oncogenes during cell transformation: interaction between activated ras and HPV-16 E7 | journal = Oncogene | volume = 20 | issue = 47 | pages = 6891–8 | date = Oct 2001 | pmid = 11687968 | doi = 10.1038/sj.onc.1204792 }}</ref><ref name="christofk2008]">{{cite journal | vauthors = Christofk HR, Vander Heiden MG, Wu N, Asara JM, Cantley LC | title = Pyruvate kinase M2 is a phosphotyrosine-binding protein | journal = Nature | volume = 452 | issue = 7184 | pages = 181–6 | date = Mar 2008 | pmid = 18337815 | doi = 10.1038/nature06667 }}</ref>
PKM2 is a [[cytosol]]ic enzyme that is associated with other glycolytic enzymes, i.e., [[hexokinase]], [[glyceraldehyde 3-phosphate dehydrogenase|glyceraldehyde 3-P dehydrogenase]], [[phosphoglycerate kinase]], [[phosphoglyceromutase]], [[enolase]], and [[lactate dehydrogenase]] within a so-called glycolytic enzyme complex.<ref name="Mazurek2005"/><ref name="Zwerschke">{{cite journal | vauthors = Zwerschke W, Mazurek S, Massimi P, Banks L, Eigenbrodt E, Jansen-Dürr P | title = Modulation of type M2 pyruvate kinase activity by the human papillomavirus type 16 E7 oncoprotein | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 96 | issue = 4 | pages = 1291–6 | date = February 1999 | pmid = 9990017 | pmc = 15456 | doi = 10.1073/pnas.96.4.1291 }}</ref><ref name="Mazurek2001">{{cite journal | vauthors = Mazurek S, Zwerschke W, Jansen-Dürr P, Eigenbrodt E | title = Metabolic cooperation between different oncogenes during cell transformation: interaction between activated ras and HPV-16 E7 | journal = Oncogene | volume = 20 | issue = 47 | pages = 6891–8 | date = October 2001 | pmid = 11687968 | doi = 10.1038/sj.onc.1204792 }}</ref><ref name="christofk2008]">{{cite journal | vauthors = Christofk HR, Vander Heiden MG, Wu N, Asara JM, Cantley LC | title = Pyruvate kinase M2 is a phosphotyrosine-binding protein | journal = Nature | volume = 452 | issue = 7184 | pages = 181–6 | date = March 2008 | pmid = 18337815 | doi = 10.1038/nature06667 }}</ref>


However, PKM2 contains an inducible [[nuclear localization signal]] in its C-terminal domain. The role of PKM2 within the [[Cell nucleus|nucleus]] is complex, since pro-proliferative but also pro-[[apoptotic]] stimuli have been described.  On the one hand, nuclear PKM2 was found to participate in the phosphorylation of [[histone]] 1 by direct phosphate transfer from PEP to histone 1. On the other hand, nuclear translocation of PKM2 induced by a [[somatostatin]] analogue, H<sub>2</sub>O<sub>2</sub>, or UV light has been linked with [[caspase]]-independent programmed cell death.<ref name="Ignacak">{{cite journal | vauthors = Ignacak J, Stachurska MB | title = The dual activity of pyruvate kinase type M2 from chromatin extracts of neoplastic cells | journal = Comparative Biochemistry and Physiology B | volume = 134 | issue = 3 | pages = 425–33 | date = Mar 2003 | pmid = 12628374 | doi = 10.1016/S1096-4959(02)00283-X }}</ref><ref name="Hoshino">{{cite journal | vauthors = Hoshino A, Hirst JA, Fujii H | title = Regulation of cell proliferation by interleukin-3-induced nuclear translocation of pyruvate kinase | journal = The Journal of Biological Chemistry | volume = 282 | issue = 24 | pages = 17706–11 | date = Jun 2007 | pmid = 17446165 | doi = 10.1074/jbc.M700094200 }}</ref><ref name="Stetak">{{cite journal | vauthors = Steták A, Veress R, Ovádi J, Csermely P, Kéri G, Ullrich A | title = Nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death | journal = Cancer Research | volume = 67 | issue = 4 | pages = 1602–8 | date = Feb 2007 | pmid = 17308100 | doi = 10.1158/0008-5472.CAN-06-2870 }}</ref>
However, PKM2 contains an inducible [[nuclear localization signal]] in its C-terminal domain. The role of PKM2 within the [[Cell nucleus|nucleus]] is complex, since pro-proliferative but also pro-[[apoptotic]] stimuli have been described.  On the one hand, nuclear PKM2 was found to participate in the phosphorylation of [[histone]] 1 by direct phosphate transfer from PEP to histone 1. On the other hand, nuclear translocation of PKM2 induced by a [[somatostatin]] analogue, H<sub>2</sub>O<sub>2</sub>, or UV light has been linked with [[caspase]]-independent programmed cell death.<ref name="Ignacak">{{cite journal | vauthors = Ignacak J, Stachurska MB | title = The dual activity of pyruvate kinase type M2 from chromatin extracts of neoplastic cells | journal = Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology | volume = 134 | issue = 3 | pages = 425–33 | date = March 2003 | pmid = 12628374 | doi = 10.1016/S1096-4959(02)00283-X }}</ref><ref name="Hoshino">{{cite journal | vauthors = Hoshino A, Hirst JA, Fujii H | title = Regulation of cell proliferation by interleukin-3-induced nuclear translocation of pyruvate kinase | journal = The Journal of Biological Chemistry | volume = 282 | issue = 24 | pages = 17706–11 | date = June 2007 | pmid = 17446165 | doi = 10.1074/jbc.M700094200 }}</ref><ref name="Stetak">{{cite journal | vauthors = Steták A, Veress R, Ovádi J, Csermely P, Kéri G, Ullrich A | title = Nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death | journal = Cancer Research | volume = 67 | issue = 4 | pages = 1602–8 | date = February 2007 | pmid = 17308100 | doi = 10.1158/0008-5472.CAN-06-2870 }}</ref>


== Clinical significance ==
== Clinical significance ==
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=== Bi-functional role within tumors ===
=== Bi-functional role within tumors ===


PKM2 is expressed in most human tumors.<ref name="Reinacher"/><ref name="Brinck"/><ref name="Steinberg"/> Initially, a switch from PKM1 to PKM2 expression during [[tumorigenesis]] was discussed.<ref name="Christofk2008b">{{cite journal | vauthors = Christofk HR, Vander Heiden MG, Harris MH, Ramanathan A, Gerszten RE, Wei R, Fleming MD, Schreiber SL, Cantley LC | title = The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth | journal = Nature | volume = 452 | issue = 7184 | pages = 230–3 | date = Mar 2008 | pmid = 18337823 | doi = 10.1038/nature06734 }}</ref> These conclusions, however, were the result of misinterpretation of [[western blot]]s that had used PKM1-expressing mouse muscle as the sole non-cancer tissue. In clinical cancer samples, solely an up-regulation of PKM2, but no cancer specificity, could be confirmed.<ref name="pmid21789790">{{cite journal | vauthors = Bluemlein K, Grüning NM, Feichtinger RG, Lehrach H, Kofler B, Ralser M | title = No evidence for a shift in pyruvate kinase PKM1 to PKM2 expression during tumorigenesis | journal = Oncotarget | volume = 2 | issue = 5 | pages = 393–400 | date = May 2011 | pmid = 21789790 | pmc = 3248187 | doi = 10.18632/oncotarget.278}}</ref>
PKM2 is expressed in most human tumors.<ref name="Reinacher"/><ref name="Brinck"/><ref name="Steinberg"/> Initially, a switch from PKM1 to PKM2 expression during [[tumorigenesis]] was discussed.<ref name="Christofk2008b">{{cite journal | vauthors = Christofk HR, Vander Heiden MG, Harris MH, Ramanathan A, Gerszten RE, Wei R, Fleming MD, Schreiber SL, Cantley LC | title = The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth | journal = Nature | volume = 452 | issue = 7184 | pages = 230–3 | date = March 2008 | pmid = 18337823 | doi = 10.1038/nature06734 }}</ref> These conclusions, however, were the result of misinterpretation of [[western blot]]s that had used PKM1-expressing mouse muscle as the sole non-cancer tissue. In clinical cancer samples, solely an up-regulation of PKM2, but no cancer specificity, could be confirmed.<ref name="pmid21789790">{{cite journal | vauthors = Bluemlein K, Grüning NM, Feichtinger RG, Lehrach H, Kofler B, Ralser M | title = No evidence for a shift in pyruvate kinase PKM1 to PKM2 expression during tumorigenesis | journal = Oncotarget | volume = 2 | issue = 5 | pages = 393–400 | date = May 2011 | pmid = 21789790 | pmc = 3248187 | doi = 10.18632/oncotarget.278 }}</ref>


In contrast to the closely homologous PKM1, which always occurs in a highly active [[tetramer]]ic form and which is not [[allosteric]]ally regulated, PKM2 may occur in a tetrameric form but also in a [[Protein dimer|dimer]]ic form.  The '''tetrameric form of PKM2''' has a high affinity to its substrate phosphoenolpyruvate (PEP), and is highly active at physiological PEP concentrations. When PKM2 is mainly in the highly active tetrameric form, which is the case in differentiated tissues and most normal proliferating cells, glucose is converted to pyruvate under the production of energy. Meanwhile, the '''dimeric form of PKM2''' is characterized by a low affinity to its substrate PEP and is nearly inactive at physiological PEP concentrations. When PKM2 is mainly in the less active dimeric form, which is the case in tumor cells, all glycolytic intermediates above pyruvate kinase accumulate and are channelled into synthetic processes, which branch off from glycolytic intermediates such as nucleic acid-, phospholipid-, and amino acid synthesis.<ref name="Eigenbrodt1980"/><ref name="Eigenbrodt1992"/><ref name="Mazurek2005"/> [[Nucleic acids]], [[phospholipids]], and [[amino acids]] are important cell building-blocks, which are greatly needed by highly proliferating cells, such as tumor cells.
In contrast to the closely homologous PKM1, which always occurs in a highly active [[tetramer]]ic form and which is not [[allosteric]]ally regulated, PKM2 may occur in a tetrameric form but also in a [[Protein dimer|dimer]]ic form.  The '''tetrameric form of PKM2''' has a high affinity to its substrate phosphoenolpyruvate (PEP), and is highly active at physiological PEP concentrations. When PKM2 is mainly in the highly active tetrameric form, which is the case in differentiated tissues and most normal proliferating cells, glucose is converted to pyruvate under the production of energy. Meanwhile, the '''dimeric form of PKM2''' is characterized by a low affinity to its substrate PEP and is nearly inactive at physiological PEP concentrations. When PKM2 is mainly in the less active dimeric form, which is the case in tumor cells, all glycolytic intermediates above pyruvate kinase accumulate and are channelled into synthetic processes, which branch off from glycolytic intermediates such as nucleic acid-, phospholipid-, and amino acid synthesis.<ref name="Eigenbrodt1980"/><ref name="Eigenbrodt1992"/><ref name="Mazurek2005"/> [[Nucleic acids]], [[phospholipids]], and [[amino acids]] are important cell building-blocks, which are greatly needed by highly proliferating cells, such as tumor cells.
Line 36: Line 36:
Due to the key position of pyruvate kinase within glycolysis, the tetramer:dimer ratio of PKM2 determines whether glucose carbons are converted to pyruvate and lactate under the production of energy (tetrameric form) or channelled into synthetic processes (dimeric form).<ref name="Eigenbrodt1980"/><ref name="Eigenbrodt1992"/><ref name="Mazurek2005"/>
Due to the key position of pyruvate kinase within glycolysis, the tetramer:dimer ratio of PKM2 determines whether glucose carbons are converted to pyruvate and lactate under the production of energy (tetrameric form) or channelled into synthetic processes (dimeric form).<ref name="Eigenbrodt1980"/><ref name="Eigenbrodt1992"/><ref name="Mazurek2005"/>


In tumor cells, PKM2 is mainly in the dimeric form and has, therefore, been termed [[Tumor M2-PK]]. The quantification of Tumor M2-PK in plasma and stool is a tool for early detection of tumors and follow-up studies during therapy. The dimerization of PKM2 in tumor cells is induced by direct interaction of PKM2 with different [[oncoprotein]]s (pp60v-src, HPV-16 E7, and A-Raf).<ref name="Zwerschke"/><ref name="Mazurek2001"/><ref name="Oude Weernink">{{cite journal | vauthors = Oude Weernink PA, Rijksen G, Staal GE | title = Phosphorylation of pyruvate kinase and glycolytic metabolism in three human glioma cell lines | journal = Tumour Biology | volume = 12 | issue = 6 | pages = 339–352 | year = 1991 | pmid = 1798909 | doi = 10.1159/000217735 }}</ref><ref name="Eigenbrodt1998">{{cite book |vauthors=Eigenbrodt E, Mazurek S, Friis RR | title = Double role of pyruvate kinase type M2 in the regulation of phosphometabolite pools. In: Bannasch P, Kanduc D, Papa S, Tager JM (eds) | journal = Cell growth and Oncogenesis | year = 1998 | publisher = Birkhäuser Verlag | location = Basel/Switzerland | volume = | pages = 15–30 | isbn = 3-7643-5727-4 | doi=10.1007/978-3-0348-8950-6_2}}</ref><ref name="Mazurek2007">{{cite journal | vauthors = Mazurek S, Drexler HC, Troppmair J, Eigenbrodt E, Rapp UR | title = Regulation of pyruvate kinase type M2 by A-Raf: a possible glycolytic stop or go mechanism | journal = Anticancer Research | volume = 27 | issue = 6B | pages = 3963–3971 | year = 2007 | pmid = 18225557 }}</ref>  The physiological function of the interaction between PKM2 and HERC1 as well as between PKM2 and PKCdelta is unknown).<ref name="Garcia">{{cite journal | vauthors = Garcia-Gonzalo FR, Cruz C, Muñoz P, Mazurek S, Eigenbrodt E, Ventura F, Bartrons R, Rosa JL | title = Interaction between HERC1 and M2-type pyruvate kinase | journal = FEBS Letters | volume = 539 | issue = 1–3 | pages = 78–84 | date = Mar 2003 | pmid = 12650930 | doi = 10.1016/S0014-5793(03)00205-9 }}</ref><ref name="Siwko">{{cite journal | vauthors = Siwko S, Mochly-Rosen D | title = Use of a novel method to find substrates of protein kinase C delta identifies M2 pyruvate kinase | journal = The International Journal of Biochemistry & Cell Biology | volume = 39 | issue = 5 | pages = 978–87 | year = 2007 | pmid = 17337233 | pmc = 1931518 | doi = 10.1016/j.biocel.2007.01.018 }}</ref>
In tumor cells, PKM2 is mainly in the dimeric form and has, therefore, been termed [[Tumor M2-PK]]. The quantification of Tumor M2-PK in plasma and stool is a tool for early detection of tumors and follow-up studies during therapy. The dimerization of PKM2 in tumor cells is induced by direct interaction of PKM2 with different [[oncoprotein]]s (pp60v-src, HPV-16 E7, and A-Raf).<ref name="Zwerschke"/><ref name="Mazurek2001"/><ref name="Oude Weernink">{{cite journal | vauthors = Oude Weernink PA, Rijksen G, Staal GE | title = Phosphorylation of pyruvate kinase and glycolytic metabolism in three human glioma cell lines | journal = Tumour Biology | volume = 12 | issue = 6 | pages = 339–52 | year = 1991 | pmid = 1798909 | doi = 10.1159/000217735 }}</ref><ref name="Eigenbrodt1998">{{cite book |vauthors=Eigenbrodt E, Mazurek S, Friis RR | title = Double role of pyruvate kinase type M2 in the regulation of phosphometabolite pools. In: Bannasch P, Kanduc D, Papa S, Tager JM (eds) | journal = Cell growth and Oncogenesis | year = 1998 | publisher = Birkhäuser Verlag | location = Basel/Switzerland | volume = | pages = 15–30 | isbn = 3-7643-5727-4 | doi=10.1007/978-3-0348-8950-6_2}}</ref><ref name="Mazurek2007">{{cite journal | vauthors = Mazurek S, Drexler HC, Troppmair J, Eigenbrodt E, Rapp UR | title = Regulation of pyruvate kinase type M2 by A-Raf: a possible glycolytic stop or go mechanism | journal = Anticancer Research | volume = 27 | issue = 6B | pages = 3963–71 | year = 2007 | pmid = 18225557 }}</ref>  The physiological function of the interaction between PKM2 and HERC1 as well as between PKM2 and PKCdelta is unknown).<ref name="Garcia">{{cite journal | vauthors = Garcia-Gonzalo FR, Cruz C, Muñoz P, Mazurek S, Eigenbrodt E, Ventura F, Bartrons R, Rosa JL | title = Interaction between HERC1 and M2-type pyruvate kinase | journal = FEBS Letters | volume = 539 | issue = 1-3 | pages = 78–84 | date = March 2003 | pmid = 12650930 | doi = 10.1016/S0014-5793(03)00205-9 }}</ref><ref name="Siwko">{{cite journal | vauthors = Siwko S, Mochly-Rosen D | title = Use of a novel method to find substrates of protein kinase C delta identifies M2 pyruvate kinase | journal = The International Journal of Biochemistry & Cell Biology | volume = 39 | issue = 5 | pages = 978–87 | year = 2007 | pmid = 17337233 | pmc = 1931518 | doi = 10.1016/j.biocel.2007.01.018 }}</ref>


However, the tetramer:dimer ratio of PKM2 is not stationary value. High levels of the glycolytic intermediate [[fructose 1,6-bisphosphate]] induce the re-association of the dimeric form of PKM2 to the tetrameric form. As a consequence, glucose is converted to pyruvate and [[lactic acid|lactate]] with the production of energy until fructose 1,6-bisphosphate levels drop below a critical value to allow dissociation to the dimeric form. This regulation is termed ''metabolic budget system''.<ref name="Eigenbrodt1992"/><ref name ="Mazurek2005"/><ref name="Ashizawa">{{cite journal | vauthors = Ashizawa K, Willingham MC, Liang CM, Cheng SY | title = In vivo regulation of monomer-tetramer conversion of pyruvate kinase subtype M2 by glucose is mediated via fructose 1,6-bisphosphate | journal = The Journal of Biological Chemistry | volume = 266 | issue = 25 | pages = 16842–16846 | date = Sep 1991 | pmid = 1885610 }}</ref> Another activator of PKM2 is the amino acid [[serine]].<ref name="Eigenbrodt1992"/> The thyroid hormone 3,3´,5-triiodi-L-tyhronine ([[Triiodothyronine|T3]]) binds to the [[monomer]]ic form of PKM2 and prevents its association to the tetrameric form.<ref name="Kato">{{cite journal | vauthors = Kato H, Fukuda T, Parkison C, McPhie P, Cheng SY | title = Cytosolic thyroid hormone-binding protein is a monomer of pyruvate kinase | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 86 | issue = 20 | pages = 7861–7865 | date = Oct 1989 | pmid = 2813362 | pmc = 298171 | doi = 10.1073/pnas.86.20.7861 }}</ref>
However, the tetramer:dimer ratio of PKM2 is not stationary value. High levels of the glycolytic intermediate [[fructose 1,6-bisphosphate]] induce the re-association of the dimeric form of PKM2 to the tetrameric form. As a consequence, glucose is converted to pyruvate and [[lactic acid|lactate]] with the production of energy until fructose 1,6-bisphosphate levels drop below a critical value to allow dissociation to the dimeric form. This regulation is termed ''metabolic budget system''.<ref name="Eigenbrodt1992"/><ref name ="Mazurek2005"/><ref name="Ashizawa">{{cite journal | vauthors = Ashizawa K, Willingham MC, Liang CM, Cheng SY | title = In vivo regulation of monomer-tetramer conversion of pyruvate kinase subtype M2 by glucose is mediated via fructose 1,6-bisphosphate | journal = The Journal of Biological Chemistry | volume = 266 | issue = 25 | pages = 16842–6 | date = September 1991 | pmid = 1885610 }}</ref> Another activator of PKM2 is the amino acid [[serine]].<ref name="Eigenbrodt1992"/> The thyroid hormone 3,3´,5-triiodi-L-tyhronine ([[Triiodothyronine|T3]]) binds to the [[monomer]]ic form of PKM2 and prevents its association to the tetrameric form.<ref name="Kato">{{cite journal | vauthors = Kato H, Fukuda T, Parkison C, McPhie P, Cheng SY | title = Cytosolic thyroid hormone-binding protein is a monomer of pyruvate kinase | journal = Proceedings of the National Academy of Sciences of the United States of America | volume = 86 | issue = 20 | pages = 7861–5 | date = October 1989 | pmid = 2813362 | pmc = 298171 | doi = 10.1073/pnas.86.20.7861 }}</ref>


In tumor cells, the increased rate of lactate production in the presence of oxygen is termed the [[Warburg effect]]. Genetic manipulation of cancer cells so that they produce adult PKM1 instead of PKM2 reverses the Warburg effect and reduces the growth rate of these modified cancer cells.<ref name="Christofk2008b" /> Accordingly, cotransfection of NIH 3T3 cells with gag-A-Raf and a kinase dead mutant of PKM2 reduced colony whereas cotransfection with gag-A-Raf and [[wild type]] PKM2 led to a doubling of focus formation.<ref name="LeMellay">{{cite journal | vauthors = Le Mellay V, Houben R, Troppmair J, Hagemann C, Mazurek S, Frey U, Beigel J, Weber C, Benz R, Eigenbrodt E, Rapp UR | title = Regulation of glycolysis by Raf protein serine/threonine kinases | journal = Advances in Enzyme Regulation | volume = 42 | issue =  | pages = 317–32 | year = 2002 | pmid = 12123723 | doi = 10.1016/S0065-2571(01)00036-X }}</ref>
In tumor cells, the increased rate of lactate production in the presence of oxygen is termed the [[Warburg effect (oncology)|Warburg effect]]. Genetic manipulation of cancer cells so that they produce adult PKM1 instead of PKM2 reverses the Warburg effect and reduces the growth rate of these modified cancer cells.<ref name="Christofk2008b" /> Accordingly, cotransfection of NIH 3T3 cells with gag-A-Raf and a kinase dead mutant of PKM2 reduced colony whereas cotransfection with gag-A-Raf and [[wild type]] PKM2 led to a doubling of focus formation.<ref name="LeMellay">{{cite journal | vauthors = Le Mellay V, Houben R, Troppmair J, Hagemann C, Mazurek S, Frey U, Beigel J, Weber C, Benz R, Eigenbrodt E, Rapp UR | title = Regulation of glycolysis by Raf protein serine/threonine kinases | journal = Advances in Enzyme Regulation | volume = 42 | issue =  | pages = 317–32 | year = 2002 | pmid = 12123723 | doi = 10.1016/S0065-2571(01)00036-X }}</ref>
 
The dimeric form of PKM2 has been observed to have protein kinase activity in tumor cells. It is able to bind to and phosphorylate the histone H3 of chromatin in cancer cells, thereby having a role in the regulation of gene expression.<ref name=":02">{{cite journal | vauthors = Yang W, Xia Y, Hawke D, Li X, Liang J, Xing D, Aldape K, Hunter T, Alfred Yung WK, Lu Z | title = PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis | journal = Cell | volume = 150 | issue = 4 | pages = 685–96 | date = August 2012 | pmid = 22901803 | doi = 10.1016/j.cell.2012.07.018 | url = https://www.sciencedirect.com/science/article/pii/S0092867412008823 }}</ref> This modification of histone H3 and the resulting involvement in gene expression regulation can be a cause of tumor cell proliferation.<ref name=":02" />
 
The pyruvate kinase activity of PKM2 can be promoted by SAICAR (succinylaminoimidazolecarboxamide ribose-5′-phosphate), an intermediate in purine biosynthesis. In cancer cells, glucose starvation leads to a rise in SAICAR levels and the subsequent stimulation of pyruvate kinase activity of PKM2. This allows for the completion of the glycolytic pathway to produce pyruvate and, therefore, survival under glucose deprivation.<ref name=":0">{{cite journal | vauthors = Keller KE, Tan IS, Lee YS | title = SAICAR stimulates pyruvate kinase isoform M2 and promotes cancer cell survival in glucose-limited conditions | journal = Science | volume = 338 | issue = 6110 | pages = 1069–72 | date = November 2012 | pmid = 23086999 | pmc = 3527123 | doi = 10.1126/science.1224409 }}</ref> In addition, an abundance of SAICAR can modify glucose absorption and lactate production in cancer cells.<ref name=":0" /> However, it has been shown that SAICAR binding also sufficiently stimulates the protein kinase activity of PKM2 in tumor cells.<ref name=":1">{{cite journal | vauthors = Keller KE, Doctor ZM, Dwyer ZW, Lee YS | title = SAICAR induces protein kinase activity of PKM2 that is necessary for sustained proliferative signaling of cancer cells | language = English | journal = Molecular Cell | volume = 53 | issue = 5 | pages = 700–9 | date = March 2014 | pmid = 24606918 | doi = 10.1016/j.molcel.2014.02.015 | url = https://www.cell.com/molecular-cell/fulltext/S1097-2765(14)00160-9?_returnURL=https://linkinghub.elsevier.com/retrieve/pii/S1097276514001609?showall=true }}</ref> In turn, the SAICAR-PKM2 complex can potentially phosphorylate a number of other protein kinases using PEP as the phosphate donor. Many of these proteins contribute to the regulation of cancer cell proliferation. Specifically, PKM2 can be a component in mitogen-activated protein kinase (MAPK) signaling, which is associated with increased cell proliferation if functioning improperly. This provides a potential link between SAICAR-activated PKM2 and cancer cell growth.<ref name=":1" />


=== Natural mutations and carcinogenesis===
=== Natural mutations and carcinogenesis===


Two [[missense mutation]]s, H391Y and K422R, of PKM2 were found in cells from [[Bloom syndrome]] patients prone to developing cancer. Results show that, despite the presence of mutations in the inter-subunit contact domain, the K422R and H391Y mutant proteins maintained their homotetrameric structure, similar to the wild-type protein, but showed a loss of activity of 75 and 20%, respectively. Interestingly, H391Y showed a 6-fold increase in affinity for its substrate phosphoenolpyruvate and behaved like a non-allosteric protein with compromised cooperative binding. However, the affinity for phosphoenolpyruvate was lost significantly in K422R. Unlike K422R, H391Y showed enhanced thermal stability, stability over a range of [[pH]] values, a lesser effect of the allosteric inhibitor Phe, and resistance toward structural alteration upon binding of the activator (fructose 1,6-bisphosphate) and inhibitor (Phe). Both mutants showed a slight shift in the pH optimum from 7.4 to 7.0.<ref name="pmid19265196">{{cite journal | vauthors = Akhtar K, Gupta V, Koul A, Alam N, Bhat R, Bamezai RN | title = Differential behavior of missense mutations in the intersubunit contact domain of the human pyruvate kinase M2 isozyme | journal = The Journal of Biological Chemistry | volume = 284 | issue = 18 | pages = 11971–81 | date = May 2009 | pmid = 19265196 | pmc = 2673266 | doi = 10.1074/jbc.M808761200 }}</ref> The co-expression of homotetrameric wild type and mutant PKM2 in the cellular milieu resulting in the interaction between the two at the monomer level was substantiated further by in vitro experiments. The cross-monomer interaction significantly altered the oligomeric state of PKM2 by favoring dimerisation and heterotetramerization. In silico study provided an added support in showing that hetero-oligomerization was energetically favorable. The hetero-oligomeric populations of PKM2 showed altered activity and affinity, and their expression resulted in an increased growth rate of Escherichia coli as well as mammalian cells, along with an increased rate of [[polyploidy]]. These features are known to be essential to tumor progression.<ref name="pmid20304929">{{cite journal | vauthors = Gupta V, Kalaiarasan P, Faheem M, Singh N, Iqbal MA, Bamezai RN | title = Dominant negative mutations affect oligomerization of human pyruvate kinase M2 isozyme and promote cellular growth and polyploidy | journal = The Journal of Biological Chemistry | volume = 285 | issue = 22 | pages = 16864–73 | date = May 2010 | pmid = 20304929 | pmc = 2878009 | doi = 10.1074/jbc.M109.065029 }}</ref>
Two [[missense mutation]]s, H391Y and K422R, of PKM2 were found in cells from [[Bloom syndrome]] patients prone to developing cancer. Results show that, despite the presence of mutations in the inter-subunit contact domain, the K422R and H391Y mutant proteins maintained their homotetrameric structure, similar to the wild-type protein, but showed a loss of activity of 75 and 20%, respectively. H391Y showed a 6-fold increase in affinity for its substrate phosphoenolpyruvate and behaved like a non-allosteric protein with compromised cooperative binding. However, the affinity for phosphoenolpyruvate was lost significantly in K422R. Unlike K422R, H391Y showed enhanced thermal stability, stability over a range of [[pH]] values, a lesser effect of the allosteric inhibitor Phe, and resistance toward structural alteration upon binding of the activator (fructose 1,6-bisphosphate) and inhibitor (Phe). Both mutants showed a slight shift in the pH optimum from 7.4 to 7.0.<ref name="pmid19265196">{{cite journal | vauthors = Akhtar K, Gupta V, Koul A, Alam N, Bhat R, Bamezai RN | title = Differential behavior of missense mutations in the intersubunit contact domain of the human pyruvate kinase M2 isozyme | journal = The Journal of Biological Chemistry | volume = 284 | issue = 18 | pages = 11971–81 | date = May 2009 | pmid = 19265196 | pmc = 2673266 | doi = 10.1074/jbc.M808761200 }}</ref> The co-expression of homotetrameric wild type and mutant PKM2 in the cellular milieu resulting in the interaction between the two at the monomer level was substantiated further by in vitro experiments. The cross-monomer interaction significantly altered the oligomeric state of PKM2 by favoring dimerisation and heterotetramerization. In silico study provided an added support in showing that hetero-oligomerization was energetically favorable. The hetero-oligomeric populations of PKM2 showed altered activity and affinity, and their expression resulted in an increased growth rate of Escherichia coli as well as mammalian cells, along with an increased rate of [[polyploidy]]. These features are known to be essential to tumor progression.<ref name="pmid20304929">{{cite journal | vauthors = Gupta V, Kalaiarasan P, Faheem M, Singh N, Iqbal MA, Bamezai RN | title = Dominant negative mutations affect oligomerization of human pyruvate kinase M2 isozyme and promote cellular growth and polyploidy | journal = The Journal of Biological Chemistry | volume = 285 | issue = 22 | pages = 16864–73 | date = May 2010 | pmid = 20304929 | pmc = 2878009 | doi = 10.1074/jbc.M109.065029 }}</ref>


Further, cells stably expressing exogenous wild- or mutant-PKM2 (K422R or H391Y) or co-expressing both wild and mutant (PKM2-K422R or PKM2-H391Y), were assessed for cancer metabolism and tumorigenic potential. Interestingly, cells co-expressing PKM2 and mutant (K422R or H391Y) showed significantly aggressive cancer metabolism, compared to cells expressing either wild or mutant PKM2 independently. A similar trend was observed for oxidative endurance, tumorigenic potential, cellular proliferation and tumor growth. These observations signify the dominant negative nature of these mutations. Remarkably, PKM2-H391Y co-expressed cells showed a maximal effect on all the studied parameters. Such a dominant negative impaired function of PKM2 in tumor development is not known; also evidencing for the first time the possible predisposition of BS patients with impaired PKM2 activity to cancer, and the importance of studying genetic variations in PKM2 in future to understand their relevance in cancer in general.<ref>{{cite journal | vauthors = Iqbal MA, Siddiqui FA, Chaman N, Gupta V, Kumar B, Gopinath P, Bamezai RN | year = 2014 | title = Missense mutations in pyruvate kinase M2 promote cancer metabolism, oxidative endurance, anchorage independence and tumor growth in a dominant negative manner | journal = J Biol Chem | volume = 289| issue = | pages = 8098–105| doi = 10.1074/jbc.M113.515742 | pmid = 24492614 | pmc=3961641}}</ref>
Further, cells stably expressing exogenous wild- or mutant-PKM2 (K422R or H391Y) or co-expressing both wild and mutant (PKM2-K422R or PKM2-H391Y), were assessed for cancer metabolism and tumorigenic potential. Cells co-expressing PKM2 and mutant (K422R or H391Y) showed significantly aggressive cancer metabolism, compared to cells expressing either wild or mutant PKM2 independently. A similar trend was observed for oxidative endurance, tumorigenic potential, cellular proliferation and tumor growth. These observations signify the dominant negative nature of these mutations. Remarkably, PKM2-H391Y co-expressed cells showed a maximal effect on all the studied parameters. Such a dominant negative impaired function of PKM2 in tumor development is not known; also evidencing for the first time the possible predisposition of BS patients with impaired PKM2 activity to cancer, and the importance of studying genetic variations in PKM2 in future to understand their relevance in cancer in general.<ref>{{cite journal | vauthors = Iqbal MA, Siddiqui FA, Chaman N, Gupta V, Kumar B, Gopinath P, Bamezai RN | title = Missense mutations in pyruvate kinase M2 promote cancer metabolism, oxidative endurance, anchorage independence, and tumor growth in a dominant negative manner | journal = The Journal of Biological Chemistry | volume = 289 | issue = 12 | pages = 8098–105 | date = March 2014 | pmid = 24492614 | pmc = 3961641 | doi = 10.1074/jbc.M113.515742 }}</ref>


=== Regulatory circuits ===
=== Regulatory circuits ===


Cancer cells are characterized by a reprogramming of energy metabolism. Over the last decade, understanding of the metabolic changes that occur in cancer has increased dramatically, and there is great interest in targeting metabolism for cancer therapy. PKM2 plays a key role in modulating glucose metabolism to support cell proliferation. PKM2, like other PK isoforms, catalyzes the last energy-generating step in glycolysis, but is unique in its capacity to be regulated. PKM2 is regulated on several cellular levels, including gene expression, alternative splicing and [[post-translational modification]]. In addition, PKM2 is regulated by key metabolic intermediates and interacts with more than twenty different proteins. Hence, this isoenzyme is an important regulator of glycolysis and additional functions in other novel roles that have recently emerged. Recent evidence indicates that intervening in the complex regulatory network of PKM2 has severe consequences on tumor cell proliferation, indicating the potential of this enzyme as a target for tumor therapy.<ref>{{cite journal | vauthors = Gupta V, Wellen KE, Mazurek S, Bamezai RN | year = 2013 | title = Pyruvate Kinase M2: Regulatory Circuits and Potential for Therapeutic Intervention | url = | journal = Curr Pharm Des. | volume = 20| issue = | pages = 2595–606| pmid = 23859618 | doi=10.2174/13816128113199990484}}</ref>
Cancer cells are characterized by a reprogramming of energy metabolism. Over the last decade, understanding of the metabolic changes that occur in cancer has increased dramatically, and there is great interest in targeting metabolism for cancer therapy. PKM2 plays a key role in modulating glucose metabolism to support cell proliferation. PKM2, like other PK isoforms, catalyzes the last energy-generating step in glycolysis, but is unique in its capacity to be regulated. PKM2 is regulated on several cellular levels, including gene expression, alternative splicing and [[post-translational modification]]. In addition, PKM2 is regulated by key metabolic intermediates and interacts with more than twenty different proteins. Hence, this isoenzyme is an important regulator of glycolysis and additional functions in other novel roles that have recently emerged. Recent evidence indicates that intervening in the complex regulatory network of PKM2 has severe consequences on tumor cell proliferation, indicating the potential of this enzyme as a target for tumor therapy.<ref>{{cite journal | vauthors = Gupta V, Wellen KE, Mazurek S, Bamezai RN | title = Pyruvate kinase M2: regulatory circuits and potential for therapeutic intervention | journal = Current Pharmaceutical Design | volume = 20 | issue = 15 | pages = 2595–606 | year = 2013 | pmid = 23859618 | doi = 10.2174/13816128113199990484 }}</ref>


=== Bacterial pathogenesis ===
=== Bacterial pathogenesis ===


With the [[yeast two-hybrid]] system, gonococcal Opa proteins were found to interact with PKM2. The results suggest that direct molecular interaction with the host metabolic enzyme PKM2 is required for the acquisition of pyruvate and for gonococcal growth and survival.<ref name="williams">{{cite journal | vauthors = Williams JM, Chen GC, Zhu L, Rest RF | title = Using the yeast two-hybrid system to identify human epithelial cell proteins that bind gonococcal Opa proteins: intracellular gonococci bind pyruvate kinase via their Opa proteins and require host pyruvate for growth | journal = Molecular Microbiology | volume = 27 | issue = 1 | pages = 171–86 | date = Jan 1998 | pmid = 9466265 | doi = 10.1046/j.1365-2958.1998.00670.x }}</ref>
With the [[yeast two-hybrid]] system, gonococcal Opa proteins were found to interact with PKM2. The results suggest that direct molecular interaction with the host metabolic enzyme PKM2 is required for the acquisition of pyruvate and for gonococcal growth and survival.<ref name="williams">{{cite journal | vauthors = Williams JM, Chen GC, Zhu L, Rest RF | title = Using the yeast two-hybrid system to identify human epithelial cell proteins that bind gonococcal Opa proteins: intracellular gonococci bind pyruvate kinase via their Opa proteins and require host pyruvate for growth | journal = Molecular Microbiology | volume = 27 | issue = 1 | pages = 171–86 | date = January 1998 | pmid = 9466265 | doi = 10.1046/j.1365-2958.1998.00670.x }}</ref>


== Interactive pathway map ==
== Interactive pathway map ==
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== External links ==
== External links ==
* {{MeshName|Pyruvate+kinase}}
* {{MeshName|Pyruvate+kinase}}
* {{cite web | url = http://www.metabolic-database.com/html/m2-pk.html | title = Pyruvate kinase isoenzyme type M2 (M2-PK) | accessdate = 2008-03-22 | author =  Erich Eigenbrodt | authorlink = |author2=Sybille Mazurek  | work = Tumor metabolome database | publisher = | pages = | archiveurl = | archivedate = | quote = }}
* {{cite web | url = http://www.metabolic-database.com/html/m2-pk.html | title = Pyruvate kinase isoenzyme type M2 (M2-PK) | access-date = 2008-03-22 | author =  Erich Eigenbrodt | authorlink = |author2=Sybille Mazurek  | work = Tumor metabolome database | publisher = | pages = | archive-url = | archive-date = | quote = }}


{{PDB_Gallery|geneid=5315}}
{{PDB_Gallery|geneid=5315}}
{{Glycolysis enzymes}}
{{Glycolysis enzymes}}
{{portal bar|Metabolism}}


[[Category:Metabolism]]
[[Category:Metabolism]]
[[Category:Enzymes]]
[[Category:Enzymes]]

Latest revision as of 12:46, 9 January 2019

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Pyruvate kinase isozymes M1/M2 (PKM1/M2), also known as pyruvate kinase muscle isozyme (PKM), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an enzyme that in humans is encoded by the PKM2 gene.[1][2][3][4]

PKM2 is an isoenzyme of the glycolytic enzyme pyruvate kinase. Depending upon the different metabolic functions of the tissues, different isoenzymes of pyruvate kinase are expressed. PKM2 is expressed in some differentiated tissues, such as lung, fat tissue, retina, and pancreatic islets, as well as in all cells with a high rate of nucleic acid synthesis, such as normal proliferating cells, embryonic cells, and especially tumor cells.[5][6][7][8][9][10][11]

Structure

Two isozymes are encoded by the PKM gene: PKM1 and PKM2. The M-gene consists of 12 exons and 11 introns. PKM1 and PKM2 are different splicing products of the M-gene (exon 9 for PKM1 and exon 10 for PKM2) and solely differ in 23 amino acids within a 56-amino acid stretch (aa 378-434) at their carboxy terminus.[12][13]

Function

Pyruvate kinase catalyzes the last step within glycolysis, the dephosphorylation of phosphoenolpyruvate to pyruvate, and is responsible for net ATP production within the glycolytic sequence. In contrast to mitochondrial respiration, energy regeneration by pyruvate kinase is independent from oxygen supply and allows survival of the organs under hypoxic conditions often found in solid tumors.[14]

The involvement of this enzyme in a variety of pathways, protein–protein interactions, and nuclear transport suggests its potential to perform multiple nonglycolytic functions with diverse implications, although multidimensional role of this protein is as yet not fully explored. However, a functional role in angiogenesis the so-called process of blood vessel formation by interaction and regulation of Jmjd8 has been shown.[15][16]

Localization

Tissue

The PKM1 isozyme is expressed in organs that are strongly dependent upon a high rate of energy regeneration, such as muscle and brain.[17][18][19]

Subcellular

PKM2 is a cytosolic enzyme that is associated with other glycolytic enzymes, i.e., hexokinase, glyceraldehyde 3-P dehydrogenase, phosphoglycerate kinase, phosphoglyceromutase, enolase, and lactate dehydrogenase within a so-called glycolytic enzyme complex.[19][20][21][22]

However, PKM2 contains an inducible nuclear localization signal in its C-terminal domain. The role of PKM2 within the nucleus is complex, since pro-proliferative but also pro-apoptotic stimuli have been described. On the one hand, nuclear PKM2 was found to participate in the phosphorylation of histone 1 by direct phosphate transfer from PEP to histone 1. On the other hand, nuclear translocation of PKM2 induced by a somatostatin analogue, H2O2, or UV light has been linked with caspase-independent programmed cell death.[23][24][25]

Clinical significance

Bi-functional role within tumors

PKM2 is expressed in most human tumors.[7][10][11] Initially, a switch from PKM1 to PKM2 expression during tumorigenesis was discussed.[26] These conclusions, however, were the result of misinterpretation of western blots that had used PKM1-expressing mouse muscle as the sole non-cancer tissue. In clinical cancer samples, solely an up-regulation of PKM2, but no cancer specificity, could be confirmed.[27]

In contrast to the closely homologous PKM1, which always occurs in a highly active tetrameric form and which is not allosterically regulated, PKM2 may occur in a tetrameric form but also in a dimeric form. The tetrameric form of PKM2 has a high affinity to its substrate phosphoenolpyruvate (PEP), and is highly active at physiological PEP concentrations. When PKM2 is mainly in the highly active tetrameric form, which is the case in differentiated tissues and most normal proliferating cells, glucose is converted to pyruvate under the production of energy. Meanwhile, the dimeric form of PKM2 is characterized by a low affinity to its substrate PEP and is nearly inactive at physiological PEP concentrations. When PKM2 is mainly in the less active dimeric form, which is the case in tumor cells, all glycolytic intermediates above pyruvate kinase accumulate and are channelled into synthetic processes, which branch off from glycolytic intermediates such as nucleic acid-, phospholipid-, and amino acid synthesis.[17][18][19] Nucleic acids, phospholipids, and amino acids are important cell building-blocks, which are greatly needed by highly proliferating cells, such as tumor cells.

Due to the key position of pyruvate kinase within glycolysis, the tetramer:dimer ratio of PKM2 determines whether glucose carbons are converted to pyruvate and lactate under the production of energy (tetrameric form) or channelled into synthetic processes (dimeric form).[17][18][19]

In tumor cells, PKM2 is mainly in the dimeric form and has, therefore, been termed Tumor M2-PK. The quantification of Tumor M2-PK in plasma and stool is a tool for early detection of tumors and follow-up studies during therapy. The dimerization of PKM2 in tumor cells is induced by direct interaction of PKM2 with different oncoproteins (pp60v-src, HPV-16 E7, and A-Raf).[20][21][28][29][30] The physiological function of the interaction between PKM2 and HERC1 as well as between PKM2 and PKCdelta is unknown).[31][32]

However, the tetramer:dimer ratio of PKM2 is not stationary value. High levels of the glycolytic intermediate fructose 1,6-bisphosphate induce the re-association of the dimeric form of PKM2 to the tetrameric form. As a consequence, glucose is converted to pyruvate and lactate with the production of energy until fructose 1,6-bisphosphate levels drop below a critical value to allow dissociation to the dimeric form. This regulation is termed metabolic budget system.[18][19][33] Another activator of PKM2 is the amino acid serine.[18] The thyroid hormone 3,3´,5-triiodi-L-tyhronine (T3) binds to the monomeric form of PKM2 and prevents its association to the tetrameric form.[34]

In tumor cells, the increased rate of lactate production in the presence of oxygen is termed the Warburg effect. Genetic manipulation of cancer cells so that they produce adult PKM1 instead of PKM2 reverses the Warburg effect and reduces the growth rate of these modified cancer cells.[26] Accordingly, cotransfection of NIH 3T3 cells with gag-A-Raf and a kinase dead mutant of PKM2 reduced colony whereas cotransfection with gag-A-Raf and wild type PKM2 led to a doubling of focus formation.[35]

The dimeric form of PKM2 has been observed to have protein kinase activity in tumor cells. It is able to bind to and phosphorylate the histone H3 of chromatin in cancer cells, thereby having a role in the regulation of gene expression.[36] This modification of histone H3 and the resulting involvement in gene expression regulation can be a cause of tumor cell proliferation.[36]

The pyruvate kinase activity of PKM2 can be promoted by SAICAR (succinylaminoimidazolecarboxamide ribose-5′-phosphate), an intermediate in purine biosynthesis. In cancer cells, glucose starvation leads to a rise in SAICAR levels and the subsequent stimulation of pyruvate kinase activity of PKM2. This allows for the completion of the glycolytic pathway to produce pyruvate and, therefore, survival under glucose deprivation.[37] In addition, an abundance of SAICAR can modify glucose absorption and lactate production in cancer cells.[37] However, it has been shown that SAICAR binding also sufficiently stimulates the protein kinase activity of PKM2 in tumor cells.[38] In turn, the SAICAR-PKM2 complex can potentially phosphorylate a number of other protein kinases using PEP as the phosphate donor. Many of these proteins contribute to the regulation of cancer cell proliferation. Specifically, PKM2 can be a component in mitogen-activated protein kinase (MAPK) signaling, which is associated with increased cell proliferation if functioning improperly. This provides a potential link between SAICAR-activated PKM2 and cancer cell growth.[38]

Natural mutations and carcinogenesis

Two missense mutations, H391Y and K422R, of PKM2 were found in cells from Bloom syndrome patients prone to developing cancer. Results show that, despite the presence of mutations in the inter-subunit contact domain, the K422R and H391Y mutant proteins maintained their homotetrameric structure, similar to the wild-type protein, but showed a loss of activity of 75 and 20%, respectively. H391Y showed a 6-fold increase in affinity for its substrate phosphoenolpyruvate and behaved like a non-allosteric protein with compromised cooperative binding. However, the affinity for phosphoenolpyruvate was lost significantly in K422R. Unlike K422R, H391Y showed enhanced thermal stability, stability over a range of pH values, a lesser effect of the allosteric inhibitor Phe, and resistance toward structural alteration upon binding of the activator (fructose 1,6-bisphosphate) and inhibitor (Phe). Both mutants showed a slight shift in the pH optimum from 7.4 to 7.0.[39] The co-expression of homotetrameric wild type and mutant PKM2 in the cellular milieu resulting in the interaction between the two at the monomer level was substantiated further by in vitro experiments. The cross-monomer interaction significantly altered the oligomeric state of PKM2 by favoring dimerisation and heterotetramerization. In silico study provided an added support in showing that hetero-oligomerization was energetically favorable. The hetero-oligomeric populations of PKM2 showed altered activity and affinity, and their expression resulted in an increased growth rate of Escherichia coli as well as mammalian cells, along with an increased rate of polyploidy. These features are known to be essential to tumor progression.[40]

Further, cells stably expressing exogenous wild- or mutant-PKM2 (K422R or H391Y) or co-expressing both wild and mutant (PKM2-K422R or PKM2-H391Y), were assessed for cancer metabolism and tumorigenic potential. Cells co-expressing PKM2 and mutant (K422R or H391Y) showed significantly aggressive cancer metabolism, compared to cells expressing either wild or mutant PKM2 independently. A similar trend was observed for oxidative endurance, tumorigenic potential, cellular proliferation and tumor growth. These observations signify the dominant negative nature of these mutations. Remarkably, PKM2-H391Y co-expressed cells showed a maximal effect on all the studied parameters. Such a dominant negative impaired function of PKM2 in tumor development is not known; also evidencing for the first time the possible predisposition of BS patients with impaired PKM2 activity to cancer, and the importance of studying genetic variations in PKM2 in future to understand their relevance in cancer in general.[41]

Regulatory circuits

Cancer cells are characterized by a reprogramming of energy metabolism. Over the last decade, understanding of the metabolic changes that occur in cancer has increased dramatically, and there is great interest in targeting metabolism for cancer therapy. PKM2 plays a key role in modulating glucose metabolism to support cell proliferation. PKM2, like other PK isoforms, catalyzes the last energy-generating step in glycolysis, but is unique in its capacity to be regulated. PKM2 is regulated on several cellular levels, including gene expression, alternative splicing and post-translational modification. In addition, PKM2 is regulated by key metabolic intermediates and interacts with more than twenty different proteins. Hence, this isoenzyme is an important regulator of glycolysis and additional functions in other novel roles that have recently emerged. Recent evidence indicates that intervening in the complex regulatory network of PKM2 has severe consequences on tumor cell proliferation, indicating the potential of this enzyme as a target for tumor therapy.[42]

Bacterial pathogenesis

With the yeast two-hybrid system, gonococcal Opa proteins were found to interact with PKM2. The results suggest that direct molecular interaction with the host metabolic enzyme PKM2 is required for the acquisition of pyruvate and for gonococcal growth and survival.[43]

Interactive pathway map

Click on genes, proteins and metabolites below to link to respective articles. [§ 1]

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Glycolysis and Gluconeogenesis edit
  1. The interactive pathway map can be edited at WikiPathways: "GlycolysisGluconeogenesis_WP534".

See also

References

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  2. Tsutsumi H, Tani K, Fujii H, Miwa S (January 1988). "Expression of L- and M-type pyruvate kinase in human tissues". Genomics. 2 (1): 86–9. doi:10.1016/0888-7543(88)90112-7. PMID 2838416.
  3. Tani K, Yoshida MC, Satoh H, Mitamura K, Noguchi T, Tanaka T, Fujii H, Miwa S (December 1988). "Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma". Gene. 73 (2): 509–16. doi:10.1016/0378-1119(88)90515-X. PMID 2854097.
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External links