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- 20:02, 14 November 2014 Jesus Hernandez talk contribs uploaded File:Entamoeba histolytica11.jpeg (Preserved using polyvinyl alcohol (PVA), this fixed trichrome-stained specimen of a liver tissue aspirate revealed a parasitic Entamoeba histolytica trophozoite. Note the prominent single round nucleus with its diffuse, centrally-placed karyosome, and ...)