Edman degradation

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Overview

Edman degradation, developed by Pehr Edman, is a method of sequencing amino acids in a peptide. In this method, the amino-terminal residue is labeled and cleaved from the peptide without disrupting the peptide bonds between other amino acid residues.

Mechanism for Edman Degradation

Phenylisothiocyanate is reacted with an uncharged terminal amino group, under mildly alkaline conditions, to form a phenylthiocarbamoyl derivative. Then, under acidic conditions, this derivative of the terminal amino acid is cleaved as a thiazolinone derivative. The thiazolinone amino acid is then selectively extracted into an organic solvent and treated with acid to form the more stable phenylthiohydantoin (PTH)- amino acid derivative that can be identified by using chromatography or electrophoresis. This procedure can then be repeated again to identify the next amino acid. A major drawback to this technique is that the peptides being sequenced in this manner cannot have more than 50 to 60 residues. This is because the Edman degradation reaction is not 100% efficient, meaning that the cleavage step does not occur every time. However, this problem can be resolved by cleaving large peptides into smaller peptides before proceeding with the reaction. It is able to accurately sequence up to 30 amino acids with 98% efficiency per amino acid. An advantage of the Edman degradation is that it only uses 10 - 100 picomoles of peptide for the sequencing process. Edman degradation reaction is automated to speed up the process.

Bergmann Degradation

An older related method for the stepwise degradation of polypeptides involving azides is the Bergmann Degradation .

References

Cost Effectiveness of Edman degradation

| group5 = Clinical Trials Involving Edman degradation | list5 = Ongoing Trials on Edman degradation at Clinical Trials.gov • Trial results on Edman degradation • Clinical Trials on Edman degradation at Google

| group6 = Guidelines / Policies / Government Resources (FDA/CDC) Regarding Edman degradation | list6 = US National Guidelines Clearinghouse on Edman degradation • NICE Guidance on Edman degradation • NHS PRODIGY Guidance • FDA on Edman degradation • CDC on Edman degradation

| group7 = Textbook Information on Edman degradation | list7 = Books and Textbook Information on Edman degradation

| group8 = Pharmacology Resources on Edman degradation | list8 = AND (Dose)}} Dosing of Edman degradation • AND (drug interactions)}} Drug interactions with Edman degradation • AND (side effects)}} Side effects of Edman degradation • AND (Allergy)}} Allergic reactions to Edman degradation • AND (overdose)}} Overdose information on Edman degradation • AND (carcinogenicity)}} Carcinogenicity information on Edman degradation • AND (pregnancy)}} Edman degradation in pregnancy • AND (pharmacokinetics)}} Pharmacokinetics of Edman degradation •

| group9 = Genetics, Pharmacogenomics, and Proteinomics of Edman degradation | list9 = AND (pharmacogenomics)}} Genetics of Edman degradation • AND (pharmacogenomics)}} Pharmacogenomics of Edman degradation • AND (proteomics)}} Proteomics of Edman degradation

| group10 = Newstories on Edman degradation | list10 = Edman degradation in the news • Be alerted to news on Edman degradation • News trends on Edman degradation</small>

| group11 = Commentary on Edman degradation | list11 = Blogs on Edman degradation

| group12 = Patient Resources on Edman degradation | list12 = Patient resources on Edman degradation • Discussion groups on Edman degradation • Patient Handouts on Edman degradation • Directions to Hospitals Treating Edman degradation • Risk calculators and risk factors for Edman degradation

| group13 = Healthcare Provider Resources on Edman degradation | list13 = Symptoms of Edman degradation • Causes & Risk Factors for Edman degradation • Diagnostic studies for Edman degradation • Treatment of Edman degradation

| group14 = Continuing Medical Education (CME) Programs on Edman degradation | list14 = CME Programs on Edman degradation

| group15 = International Resources on Edman degradation | list15 = Edman degradation en Espanol • Edman degradation en Francais

| group16 = Business Resources on Edman degradation | list16 = Edman degradation in the Marketplace • Patents on Edman degradation

| group17 = Informatics Resources on Edman degradation | list17 = List of terms related to Edman degradation

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