Aplastic anemia diagnostic study of choice: Difference between revisions
Nazia Fuad (talk | contribs) |
Nazia Fuad (talk | contribs) |
||
| Line 25: | Line 25: | ||
** [[Flow cytometry]] for assessment of cell surface CD59 on peripheral blood red blood cells or [[Neutrophil|neutrophils]]. | ** [[Flow cytometry]] for assessment of cell surface CD59 on peripheral blood red blood cells or [[Neutrophil|neutrophils]]. | ||
** [[Cytogenetics|Cytogenetic]] and molecular testing of [[bone marrow]] | ** [[Cytogenetics|Cytogenetic]] and molecular testing of [[bone marrow]] | ||
** Hemoglobin electrophoresis and blood-group testing | |||
** Biochemical profile | |||
** Serology | |||
** Fluorescence-activated cell sorter (FACS) profiling | |||
** Fluorescent-labeled inactive toxin aerolysin (FLAER) testing | |||
** Diepoxybutane incubation | |||
** Histocompatibility testing | |||
* '''In''' '''children''' with AA genetic testing should be performed to find out inherited genetic abnormalities. | * '''In''' '''children''' with AA genetic testing should be performed to find out inherited genetic abnormalities. | ||
* Some are [[Fanconi anemia]], [[dyskeratosis congenita]] and short telomere syndromes | * Some are [[Fanconi anemia]], [[dyskeratosis congenita]] and short telomere syndromes | ||
Revision as of 16:29, 21 August 2018
|
Aplastic anemia Microchapters |
|
Diagnosis |
|---|
|
Treatment |
|
Case Studies |
|
Aplastic anemia diagnostic study of choice On the Web |
|
American Roentgen Ray Society Images of Aplastic anemia diagnostic study of choice |
|
Risk calculators and risk factors for Aplastic anemia diagnostic study of choice |
Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1]; Associate Editor(s)-in-Chief: Nazia Fuad M.D.
Overview
Bone marrow biopsy is the gold standard test for the diagnosis of aplastic anemia, These findings on performing bone marrow biopsy are confirmatory for aplastic anemiaHypoplasia with <20% cellularity, normal maturation of all cell line, fat cells and stroma in bone marrow space. Residual hematopoietic cells are morphologically normal and hematopoiesis is not megaloblastic.
Diagnostic Study of Choice
Study of choice
Bone marrow biopsy is the gold standard test for the diagnosis of aplastic anemia[1]
Diagnostic results
The following findings on performing bone marrow biopsy are confirmatory for aplastic anemia:
- Hypoplasia with <20% cellularity
- Normal maturation of all cell line
- Fat cells and stroma in bone marrow space
- Residual hematopoietic cells are morphologically normal
- Hematopoiesis is not megaloblastic
Sequence of Diagnostic Studies
Investigations must be performed in the following order:[2]
- In adults with AA, these tests should be done to detect coexistent disorders, such as paroxysmal nocturnal hemoglobinuria, myelodysplastic syndrome, or acute leukemia:
- Flow cytometry for assessment of cell surface CD59 on peripheral blood red blood cells or neutrophils.
- Cytogenetic and molecular testing of bone marrow
- Hemoglobin electrophoresis and blood-group testing
- Biochemical profile
- Serology
- Fluorescence-activated cell sorter (FACS) profiling
- Fluorescent-labeled inactive toxin aerolysin (FLAER) testing
- Diepoxybutane incubation
- Histocompatibility testing
- In children with AA genetic testing should be performed to find out inherited genetic abnormalities.
- Some are Fanconi anemia, dyskeratosis congenita and short telomere syndromes
References
- ↑ Dezern AE, Brodsky RA (April 2011). "Clinical management of aplastic anemia". Expert Rev Hematol. 4 (2): 221–30. doi:10.1586/ehm.11.11. PMC 3138728. PMID 21495931.
- ↑ D'Andrea AD, Grompe M (January 2003). "The Fanconi anaemia/BRCA pathway". Nat. Rev. Cancer. 3 (1): 23–34. doi:10.1038/nrc970. PMID 12509764.