Trichomoniasis laboratory findings: Difference between revisions

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===Culture===
===Culture===
Historically, culture has been the gold standard for diagnosis of trichomoniasis.


===Nucleic Acid Amplification Tests===
===Nucleic Acid Amplification Tests===

Revision as of 22:34, 4 February 2016

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Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1] Associate Editor(s)-in-Chief: Maliha Shakil, M.D. [2]

Overview

Laboratory tests used in the diagnosis of trichomoniasis include saline microscopy, culture, and nucleic acid amplification tests (NAATs).[1]

Laboratory Findings

Microscopy

Trichomoniasis is diagnosed by visually observing the trichomonads via a microscope. In women, the examiner collects the specimen during a pelvic examination by inserting a speculum into the vagina and then using a cotton-tipped applicator to collect the sample. The sample is then placed onto a microscopic slide and sent to a laboratory to be analyzed. Trichomoniasis has been difficult to diagnose due to the poor sensitivity of the tests.[2]

Findings on microscopy suggestive of trichomoniasis include:[3][4]

  • Characteristic "tumbling" motility of protozoa
  • Leukocytes


Trichomonas vaginalis Pap smear

Two trophozoites of Trichomonas vaginalis obtained from in vitro culture. Smear was stained with Giemsa.[5]

Culture

Historically, culture has been the gold standard for diagnosis of trichomoniasis.

Nucleic Acid Amplification Tests

References

  1. Trichomonasias . Wikipedia.https://en.wikipedia.org/wiki/Trichomoniasis Accessed on February 4, 2016
  2. Andrea SB, Chapin KC (2011). "Comparison of Aptima Trichomonas vaginalis Transcription-Mediated Amplification Assay and BD Affirm VPIII for Detection of T. vaginalis in Symptomatic Women: Performance Parameters and Epidemiological Implications". J Clin Microbiol. 49 (3): 866–9. doi:10.1128/JCM.02367-10. PMID 21248097. Lay summary.
  3. Hobbs MM, Seña AC (2013). "Modern diagnosis of Trichomonas vaginalis infection". Sex Transm Infect. 89 (6): 434–8. doi:10.1136/sextrans-2013-051057. PMC 3787709. PMID 23633669.
  4. Schwebke JR, Burgess D (2004). "Trichomoniasis". Clin Microbiol Rev. 17 (4): 794–803, table of contents. doi:10.1128/CMR.17.4.794-803.2004. PMC 523559. PMID 15489349.
  5. "DPDx - Trichomoniasis". Retrieved 2012-12-27.

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