Uncompetitive inhibitor

Uncompetitive inhibition takes place when an enzyme inhibitor binds only to the complex formed between the enzyme and the substrate (the E-S complex).

This reduction in the effective concentration of the E-S complex increases the enzyme's apparent affinity for the substrate through Le Chatelier's principle (K_m is lowered) and decreases the maximum enzyme activity (V_max), as it takes longer for the substrate or product to leave the active site. Uncompetitive inhibition works best when substrate concentration is high.

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